8EJ4

Cryo-EM structure of the active NLRP3 inflammasome disk

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
K	0000922	cellular_component	spindle pole
K	0004672	molecular_function	protein kinase activity
K	0004674	molecular_function	protein serine/threonine kinase activity
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005737	cellular_component	cytoplasm
K	0005813	cellular_component	centrosome
K	0005815	cellular_component	microtubule organizing center
K	0005856	cellular_component	cytoskeleton
K	0005874	cellular_component	microtubule
K	0006468	biological_process	protein phosphorylation
K	0007346	biological_process	regulation of mitotic cell cycle
K	0032212	biological_process	positive regulation of telomere maintenance via telomerase
K	0035865	biological_process	cellular response to potassium ion
K	0046872	molecular_function	metal ion binding
K	0051225	biological_process	spindle assembly
K	0051973	biological_process	positive regulation of telomerase activity
K	0106310	molecular_function	protein serine kinase activity
K	0140677	molecular_function	molecular function activator activity
K	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
K	1904355	biological_process	positive regulation of telomere capping
L	0000922	cellular_component	spindle pole
L	0004672	molecular_function	protein kinase activity
L	0004674	molecular_function	protein serine/threonine kinase activity
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005737	cellular_component	cytoplasm
L	0005813	cellular_component	centrosome
L	0005815	cellular_component	microtubule organizing center
L	0005856	cellular_component	cytoskeleton
L	0005874	cellular_component	microtubule
L	0006468	biological_process	protein phosphorylation
L	0007346	biological_process	regulation of mitotic cell cycle
L	0032212	biological_process	positive regulation of telomere maintenance via telomerase
L	0035865	biological_process	cellular response to potassium ion
L	0046872	molecular_function	metal ion binding
L	0051225	biological_process	spindle assembly
L	0051973	biological_process	positive regulation of telomerase activity
L	0106310	molecular_function	protein serine kinase activity
L	0140677	molecular_function	molecular function activator activity
L	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
L	1904355	biological_process	positive regulation of telomere capping
M	0000922	cellular_component	spindle pole
M	0004672	molecular_function	protein kinase activity
M	0004674	molecular_function	protein serine/threonine kinase activity
M	0005515	molecular_function	protein binding
M	0005524	molecular_function	ATP binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005737	cellular_component	cytoplasm
M	0005813	cellular_component	centrosome
M	0005815	cellular_component	microtubule organizing center
M	0005856	cellular_component	cytoskeleton
M	0005874	cellular_component	microtubule
M	0006468	biological_process	protein phosphorylation
M	0007346	biological_process	regulation of mitotic cell cycle
M	0032212	biological_process	positive regulation of telomere maintenance via telomerase
M	0035865	biological_process	cellular response to potassium ion
M	0046872	molecular_function	metal ion binding
M	0051225	biological_process	spindle assembly
M	0051973	biological_process	positive regulation of telomerase activity
M	0106310	molecular_function	protein serine kinase activity
M	0140677	molecular_function	molecular function activator activity
M	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
M	1904355	biological_process	positive regulation of telomere capping
N	0000922	cellular_component	spindle pole
N	0004672	molecular_function	protein kinase activity
N	0004674	molecular_function	protein serine/threonine kinase activity
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005737	cellular_component	cytoplasm
N	0005813	cellular_component	centrosome
N	0005815	cellular_component	microtubule organizing center
N	0005856	cellular_component	cytoskeleton
N	0005874	cellular_component	microtubule
N	0006468	biological_process	protein phosphorylation
N	0007346	biological_process	regulation of mitotic cell cycle
N	0032212	biological_process	positive regulation of telomere maintenance via telomerase
N	0035865	biological_process	cellular response to potassium ion
N	0046872	molecular_function	metal ion binding
N	0051225	biological_process	spindle assembly
N	0051973	biological_process	positive regulation of telomerase activity
N	0106310	molecular_function	protein serine kinase activity
N	0140677	molecular_function	molecular function activator activity
N	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
N	1904355	biological_process	positive regulation of telomere capping
O	0000922	cellular_component	spindle pole
O	0004672	molecular_function	protein kinase activity
O	0004674	molecular_function	protein serine/threonine kinase activity
O	0005515	molecular_function	protein binding
O	0005524	molecular_function	ATP binding
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005737	cellular_component	cytoplasm
O	0005813	cellular_component	centrosome
O	0005815	cellular_component	microtubule organizing center
O	0005856	cellular_component	cytoskeleton
O	0005874	cellular_component	microtubule
O	0006468	biological_process	protein phosphorylation
O	0007346	biological_process	regulation of mitotic cell cycle
O	0032212	biological_process	positive regulation of telomere maintenance via telomerase
O	0035865	biological_process	cellular response to potassium ion
O	0046872	molecular_function	metal ion binding
O	0051225	biological_process	spindle assembly
O	0051973	biological_process	positive regulation of telomerase activity
O	0106310	molecular_function	protein serine kinase activity
O	0140677	molecular_function	molecular function activator activity
O	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
O	1904355	biological_process	positive regulation of telomere capping
P	0000922	cellular_component	spindle pole
P	0004672	molecular_function	protein kinase activity
P	0004674	molecular_function	protein serine/threonine kinase activity
P	0005515	molecular_function	protein binding
P	0005524	molecular_function	ATP binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005737	cellular_component	cytoplasm
P	0005813	cellular_component	centrosome
P	0005815	cellular_component	microtubule organizing center
P	0005856	cellular_component	cytoskeleton
P	0005874	cellular_component	microtubule
P	0006468	biological_process	protein phosphorylation
P	0007346	biological_process	regulation of mitotic cell cycle
P	0032212	biological_process	positive regulation of telomere maintenance via telomerase
P	0035865	biological_process	cellular response to potassium ion
P	0046872	molecular_function	metal ion binding
P	0051225	biological_process	spindle assembly
P	0051973	biological_process	positive regulation of telomerase activity
P	0106310	molecular_function	protein serine kinase activity
P	0140677	molecular_function	molecular function activator activity
P	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
P	1904355	biological_process	positive regulation of telomere capping
Q	0000922	cellular_component	spindle pole
Q	0004672	molecular_function	protein kinase activity
Q	0004674	molecular_function	protein serine/threonine kinase activity
Q	0005515	molecular_function	protein binding
Q	0005524	molecular_function	ATP binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005737	cellular_component	cytoplasm
Q	0005813	cellular_component	centrosome
Q	0005815	cellular_component	microtubule organizing center
Q	0005856	cellular_component	cytoskeleton
Q	0005874	cellular_component	microtubule
Q	0006468	biological_process	protein phosphorylation
Q	0007346	biological_process	regulation of mitotic cell cycle
Q	0032212	biological_process	positive regulation of telomere maintenance via telomerase
Q	0035865	biological_process	cellular response to potassium ion
Q	0046872	molecular_function	metal ion binding
Q	0051225	biological_process	spindle assembly
Q	0051973	biological_process	positive regulation of telomerase activity
Q	0106310	molecular_function	protein serine kinase activity
Q	0140677	molecular_function	molecular function activator activity
Q	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
Q	1904355	biological_process	positive regulation of telomere capping
R	0000922	cellular_component	spindle pole
R	0004672	molecular_function	protein kinase activity
R	0004674	molecular_function	protein serine/threonine kinase activity
R	0005515	molecular_function	protein binding
R	0005524	molecular_function	ATP binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005813	cellular_component	centrosome
R	0005815	cellular_component	microtubule organizing center
R	0005856	cellular_component	cytoskeleton
R	0005874	cellular_component	microtubule
R	0006468	biological_process	protein phosphorylation
R	0007346	biological_process	regulation of mitotic cell cycle
R	0032212	biological_process	positive regulation of telomere maintenance via telomerase
R	0035865	biological_process	cellular response to potassium ion
R	0046872	molecular_function	metal ion binding
R	0051225	biological_process	spindle assembly
R	0051973	biological_process	positive regulation of telomerase activity
R	0106310	molecular_function	protein serine kinase activity
R	0140677	molecular_function	molecular function activator activity
R	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
R	1904355	biological_process	positive regulation of telomere capping
S	0000922	cellular_component	spindle pole
S	0004672	molecular_function	protein kinase activity
S	0004674	molecular_function	protein serine/threonine kinase activity
S	0005515	molecular_function	protein binding
S	0005524	molecular_function	ATP binding
S	0005634	cellular_component	nucleus
S	0005654	cellular_component	nucleoplasm
S	0005737	cellular_component	cytoplasm
S	0005813	cellular_component	centrosome
S	0005815	cellular_component	microtubule organizing center
S	0005856	cellular_component	cytoskeleton
S	0005874	cellular_component	microtubule
S	0006468	biological_process	protein phosphorylation
S	0007346	biological_process	regulation of mitotic cell cycle
S	0032212	biological_process	positive regulation of telomere maintenance via telomerase
S	0035865	biological_process	cellular response to potassium ion
S	0046872	molecular_function	metal ion binding
S	0051225	biological_process	spindle assembly
S	0051973	biological_process	positive regulation of telomerase activity
S	0106310	molecular_function	protein serine kinase activity
S	0140677	molecular_function	molecular function activator activity
S	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
S	1904355	biological_process	positive regulation of telomere capping
T	0000922	cellular_component	spindle pole
T	0004672	molecular_function	protein kinase activity
T	0004674	molecular_function	protein serine/threonine kinase activity
T	0005515	molecular_function	protein binding
T	0005524	molecular_function	ATP binding
T	0005634	cellular_component	nucleus
T	0005654	cellular_component	nucleoplasm
T	0005737	cellular_component	cytoplasm
T	0005813	cellular_component	centrosome
T	0005815	cellular_component	microtubule organizing center
T	0005856	cellular_component	cytoskeleton
T	0005874	cellular_component	microtubule
T	0006468	biological_process	protein phosphorylation
T	0007346	biological_process	regulation of mitotic cell cycle
T	0032212	biological_process	positive regulation of telomere maintenance via telomerase
T	0035865	biological_process	cellular response to potassium ion
T	0046872	molecular_function	metal ion binding
T	0051225	biological_process	spindle assembly
T	0051973	biological_process	positive regulation of telomerase activity
T	0106310	molecular_function	protein serine kinase activity
T	0140677	molecular_function	molecular function activator activity
T	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
T	1904355	biological_process	positive regulation of telomere capping

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGRGQFSEVYrAaclldgvp..........VALK

Chain	Residue	Details
K	ILE40-LYS63

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VmHrDIKpaNVFI

Chain	Residue	Details
K	VAL157-ILE169

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	10
Details	ACT_SITE: Proton acceptor => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000255|PROSITE-ProRule:PRU10027

Chain	Residue	Details
K	ASP161
T	ASP161
L	ASP161
M	ASP161
N	ASP161
O	ASP161
P	ASP161
Q	ASP161
R	ASP161
S	ASP161

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	BINDING: BINDING => ECO:0000305|PubMed:19941817, ECO:0007744|PDB:2WQN

Chain	Residue	Details
K	ILE40
O	LYS63
P	ILE40
P	LYS63
Q	ILE40
Q	LYS63
R	ILE40
R	LYS63
S	ILE40
S	LYS63
T	ILE40
K	LYS63
T	LYS63
L	ILE40
L	LYS63
M	ILE40
M	LYS63
N	ILE40
N	LYS63
O	ILE40

---

site_id	SWS_FT_FI3
Number of Residues	10
Details	SITE: Autoinhibitory => ECO:0000269|PubMed:19941817

Chain	Residue	Details
K	TYR97
T	TYR97
L	TYR97
M	TYR97
N	TYR97
O	TYR97
P	TYR97
Q	TYR97
R	TYR97
S	TYR97

---

site_id	SWS_FT_FI4
Number of Residues	10
Details	MOD_RES: Phosphoserine; by NEK9 => ECO:0000269|PubMed:12840024, ECO:0000269|PubMed:26522158

Chain	Residue	Details
K	SER195
T	SER195
C	TYR143
C	TYR168
D	TYR136
D	TYR140
D	TYR143
D	TYR168
E	TYR136
E	TYR140
E	TYR143
L	SER195
E	TYR168
F	TYR136
F	TYR140
F	TYR143
F	TYR168
G	TYR136
G	TYR140
G	TYR143
G	TYR168
H	TYR136
M	SER195
H	TYR140
H	TYR143
H	TYR168
I	TYR136
I	TYR140
I	TYR143
I	TYR168
J	TYR136
J	TYR140
J	TYR143
N	SER195
J	TYR168
O	SER195
P	SER195
Q	SER195
R	SER195
S	SER195

---

site_id	SWS_FT_FI5
Number of Residues	20
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q8R4B8

Chain	Residue	Details
A	SER161
E	SER295
F	SER161
F	SER295
G	SER161
G	SER295
H	SER161
H	SER295
I	SER161
I	SER295
J	SER161
A	SER295
J	SER295
B	SER161
B	SER295
C	SER161
C	SER295
D	SER161
D	SER295
E	SER161

---

site_id	SWS_FT_FI6
Number of Residues	40
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:28943315

Chain	Residue	Details
A	SER163
C	SER334
C	SER728
C	SER975
D	SER163
D	SER334
D	SER728
D	SER975
E	SER163
E	SER334
E	SER728
A	SER334
E	SER975
F	SER163
F	SER334
F	SER728
F	SER975
G	SER163
G	SER334
G	SER728
G	SER975
H	SER163
A	SER728
H	SER334
H	SER728
H	SER975
I	SER163
I	SER334
I	SER728
I	SER975
J	SER163
J	SER334
J	SER728
A	SER975
J	SER975
B	SER163
B	SER334
B	SER728
B	SER975
C	SER163

---

site_id	SWS_FT_FI7
Number of Residues	10
Details	MOD_RES: Phosphoserine; by MAPK8 => ECO:0000269|PubMed:35114687

Chain	Residue	Details
A	SER198
J	SER198
B	SER198
C	SER198
D	SER198
E	SER198
F	SER198
G	SER198
H	SER198
I	SER198

---

site_id	SWS_FT_FI8
Number of Residues	10
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:35114687

Chain	Residue	Details
A	SER201
J	SER201
B	SER201
C	SER201
D	SER201
E	SER201
F	SER201
G	SER201
H	SER201
I	SER201

---

site_id	SWS_FT_FI9
Number of Residues	20
Details	MOD_RES: Phosphoserine; by CSNK1A1 => ECO:0000269|PubMed:34615873

Chain	Residue	Details
A	SER735
E	SER806
F	SER735
F	SER806
G	SER735
G	SER806
H	SER735
H	SER806
I	SER735
I	SER806
J	SER735
A	SER806
J	SER806
B	SER735
B	SER806
C	SER735
C	SER806
D	SER735
D	SER806
E	SER735

---

site_id	SWS_FT_FI10
Number of Residues	10
Details	MOD_RES: Phosphotyrosine => ECO:0000269|PubMed:27043286

Chain	Residue	Details
A	TYR861
J	TYR861
B	TYR861
C	TYR861
D	TYR861
E	TYR861
F	TYR861
G	TYR861
H	TYR861
I	TYR861

---

site_id	SWS_FT_FI11
Number of Residues	30
Details	LIPID: S-palmitoyl cysteine => ECO:0000269|PubMed:36586411

Chain	Residue	Details
A	CYS837
D	CYS837
D	CYS838
D	CYS844
E	CYS837
E	CYS838
E	CYS844
F	CYS837
F	CYS838
F	CYS844
G	CYS837
A	CYS838
G	CYS838
G	CYS844
H	CYS837
H	CYS838
H	CYS844
I	CYS837
I	CYS838
I	CYS844
J	CYS837
J	CYS838
A	CYS844
J	CYS844
B	CYS837
B	CYS838
B	CYS844
C	CYS837
C	CYS838
C	CYS844

---

site_id	SWS_FT_FI12
Number of Residues	10
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:26037928

Chain	Residue	Details
A	LYS689
J	LYS689
B	LYS689
C	LYS689
D	LYS689
E	LYS689
F	LYS689
G	LYS689
H	LYS689
I	LYS689

---

site_id	SWS_FT_FI13
Number of Residues	40
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:34615873

Chain	Residue	Details
C	LYS878
C	LYS927
C	LYS973
D	LYS878
D	LYS927
D	LYS973
E	LYS878
E	LYS927
A	LYS878
E	LYS973
F	LYS878
F	LYS927
F	LYS973
G	LYS878
G	LYS927
G	LYS973
A	LYS927
H	LYS878
H	LYS927
H	LYS973
I	LYS878
I	LYS927
I	LYS973
J	LYS878
J	LYS927
A	LYS973
J	LYS973
B	LYS878
B	LYS927
B	LYS973

---