8EJ4

Cryo-EM structure of the active NLRP3 inflammasome disk

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
K	0000166	molecular_function	nucleotide binding
K	0000922	cellular_component	spindle pole
K	0004672	molecular_function	protein kinase activity
K	0004674	molecular_function	protein serine/threonine kinase activity
K	0005515	molecular_function	protein binding
K	0005524	molecular_function	ATP binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005737	cellular_component	cytoplasm
K	0005813	cellular_component	centrosome
K	0005815	cellular_component	microtubule organizing center
K	0005874	cellular_component	microtubule
K	0006468	biological_process	protein phosphorylation
K	0007346	biological_process	regulation of mitotic cell cycle
K	0016301	molecular_function	kinase activity
K	0016740	molecular_function	transferase activity
K	0032206	biological_process	positive regulation of telomere maintenance
K	0033044	biological_process	regulation of chromosome organization
K	0035865	biological_process	cellular response to potassium ion
K	0043549	biological_process	regulation of kinase activity
K	0046872	molecular_function	metal ion binding
K	0051225	biological_process	spindle assembly
K	0106310	molecular_function	protein serine kinase activity
K	0140677	molecular_function	molecular function activator activity
K	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
L	0000166	molecular_function	nucleotide binding
L	0000922	cellular_component	spindle pole
L	0004672	molecular_function	protein kinase activity
L	0004674	molecular_function	protein serine/threonine kinase activity
L	0005515	molecular_function	protein binding
L	0005524	molecular_function	ATP binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005737	cellular_component	cytoplasm
L	0005813	cellular_component	centrosome
L	0005815	cellular_component	microtubule organizing center
L	0005874	cellular_component	microtubule
L	0006468	biological_process	protein phosphorylation
L	0007346	biological_process	regulation of mitotic cell cycle
L	0016301	molecular_function	kinase activity
L	0016740	molecular_function	transferase activity
L	0032206	biological_process	positive regulation of telomere maintenance
L	0033044	biological_process	regulation of chromosome organization
L	0035865	biological_process	cellular response to potassium ion
L	0043549	biological_process	regulation of kinase activity
L	0046872	molecular_function	metal ion binding
L	0051225	biological_process	spindle assembly
L	0106310	molecular_function	protein serine kinase activity
L	0140677	molecular_function	molecular function activator activity
L	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
M	0000166	molecular_function	nucleotide binding
M	0000922	cellular_component	spindle pole
M	0004672	molecular_function	protein kinase activity
M	0004674	molecular_function	protein serine/threonine kinase activity
M	0005515	molecular_function	protein binding
M	0005524	molecular_function	ATP binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005737	cellular_component	cytoplasm
M	0005813	cellular_component	centrosome
M	0005815	cellular_component	microtubule organizing center
M	0005874	cellular_component	microtubule
M	0006468	biological_process	protein phosphorylation
M	0007346	biological_process	regulation of mitotic cell cycle
M	0016301	molecular_function	kinase activity
M	0016740	molecular_function	transferase activity
M	0032206	biological_process	positive regulation of telomere maintenance
M	0033044	biological_process	regulation of chromosome organization
M	0035865	biological_process	cellular response to potassium ion
M	0043549	biological_process	regulation of kinase activity
M	0046872	molecular_function	metal ion binding
M	0051225	biological_process	spindle assembly
M	0106310	molecular_function	protein serine kinase activity
M	0140677	molecular_function	molecular function activator activity
M	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
N	0000166	molecular_function	nucleotide binding
N	0000922	cellular_component	spindle pole
N	0004672	molecular_function	protein kinase activity
N	0004674	molecular_function	protein serine/threonine kinase activity
N	0005515	molecular_function	protein binding
N	0005524	molecular_function	ATP binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005737	cellular_component	cytoplasm
N	0005813	cellular_component	centrosome
N	0005815	cellular_component	microtubule organizing center
N	0005874	cellular_component	microtubule
N	0006468	biological_process	protein phosphorylation
N	0007346	biological_process	regulation of mitotic cell cycle
N	0016301	molecular_function	kinase activity
N	0016740	molecular_function	transferase activity
N	0032206	biological_process	positive regulation of telomere maintenance
N	0033044	biological_process	regulation of chromosome organization
N	0035865	biological_process	cellular response to potassium ion
N	0043549	biological_process	regulation of kinase activity
N	0046872	molecular_function	metal ion binding
N	0051225	biological_process	spindle assembly
N	0106310	molecular_function	protein serine kinase activity
N	0140677	molecular_function	molecular function activator activity
N	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
O	0000166	molecular_function	nucleotide binding
O	0000922	cellular_component	spindle pole
O	0004672	molecular_function	protein kinase activity
O	0004674	molecular_function	protein serine/threonine kinase activity
O	0005515	molecular_function	protein binding
O	0005524	molecular_function	ATP binding
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005737	cellular_component	cytoplasm
O	0005813	cellular_component	centrosome
O	0005815	cellular_component	microtubule organizing center
O	0005874	cellular_component	microtubule
O	0006468	biological_process	protein phosphorylation
O	0007346	biological_process	regulation of mitotic cell cycle
O	0016301	molecular_function	kinase activity
O	0016740	molecular_function	transferase activity
O	0032206	biological_process	positive regulation of telomere maintenance
O	0033044	biological_process	regulation of chromosome organization
O	0035865	biological_process	cellular response to potassium ion
O	0043549	biological_process	regulation of kinase activity
O	0046872	molecular_function	metal ion binding
O	0051225	biological_process	spindle assembly
O	0106310	molecular_function	protein serine kinase activity
O	0140677	molecular_function	molecular function activator activity
O	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
P	0000166	molecular_function	nucleotide binding
P	0000922	cellular_component	spindle pole
P	0004672	molecular_function	protein kinase activity
P	0004674	molecular_function	protein serine/threonine kinase activity
P	0005515	molecular_function	protein binding
P	0005524	molecular_function	ATP binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005737	cellular_component	cytoplasm
P	0005813	cellular_component	centrosome
P	0005815	cellular_component	microtubule organizing center
P	0005874	cellular_component	microtubule
P	0006468	biological_process	protein phosphorylation
P	0007346	biological_process	regulation of mitotic cell cycle
P	0016301	molecular_function	kinase activity
P	0016740	molecular_function	transferase activity
P	0032206	biological_process	positive regulation of telomere maintenance
P	0033044	biological_process	regulation of chromosome organization
P	0035865	biological_process	cellular response to potassium ion
P	0043549	biological_process	regulation of kinase activity
P	0046872	molecular_function	metal ion binding
P	0051225	biological_process	spindle assembly
P	0106310	molecular_function	protein serine kinase activity
P	0140677	molecular_function	molecular function activator activity
P	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
Q	0000166	molecular_function	nucleotide binding
Q	0000922	cellular_component	spindle pole
Q	0004672	molecular_function	protein kinase activity
Q	0004674	molecular_function	protein serine/threonine kinase activity
Q	0005515	molecular_function	protein binding
Q	0005524	molecular_function	ATP binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005737	cellular_component	cytoplasm
Q	0005813	cellular_component	centrosome
Q	0005815	cellular_component	microtubule organizing center
Q	0005874	cellular_component	microtubule
Q	0006468	biological_process	protein phosphorylation
Q	0007346	biological_process	regulation of mitotic cell cycle
Q	0016301	molecular_function	kinase activity
Q	0016740	molecular_function	transferase activity
Q	0032206	biological_process	positive regulation of telomere maintenance
Q	0033044	biological_process	regulation of chromosome organization
Q	0035865	biological_process	cellular response to potassium ion
Q	0043549	biological_process	regulation of kinase activity
Q	0046872	molecular_function	metal ion binding
Q	0051225	biological_process	spindle assembly
Q	0106310	molecular_function	protein serine kinase activity
Q	0140677	molecular_function	molecular function activator activity
Q	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
R	0000166	molecular_function	nucleotide binding
R	0000922	cellular_component	spindle pole
R	0004672	molecular_function	protein kinase activity
R	0004674	molecular_function	protein serine/threonine kinase activity
R	0005515	molecular_function	protein binding
R	0005524	molecular_function	ATP binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005813	cellular_component	centrosome
R	0005815	cellular_component	microtubule organizing center
R	0005874	cellular_component	microtubule
R	0006468	biological_process	protein phosphorylation
R	0007346	biological_process	regulation of mitotic cell cycle
R	0016301	molecular_function	kinase activity
R	0016740	molecular_function	transferase activity
R	0032206	biological_process	positive regulation of telomere maintenance
R	0033044	biological_process	regulation of chromosome organization
R	0035865	biological_process	cellular response to potassium ion
R	0043549	biological_process	regulation of kinase activity
R	0046872	molecular_function	metal ion binding
R	0051225	biological_process	spindle assembly
R	0106310	molecular_function	protein serine kinase activity
R	0140677	molecular_function	molecular function activator activity
R	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
S	0000166	molecular_function	nucleotide binding
S	0000922	cellular_component	spindle pole
S	0004672	molecular_function	protein kinase activity
S	0004674	molecular_function	protein serine/threonine kinase activity
S	0005515	molecular_function	protein binding
S	0005524	molecular_function	ATP binding
S	0005634	cellular_component	nucleus
S	0005654	cellular_component	nucleoplasm
S	0005737	cellular_component	cytoplasm
S	0005813	cellular_component	centrosome
S	0005815	cellular_component	microtubule organizing center
S	0005874	cellular_component	microtubule
S	0006468	biological_process	protein phosphorylation
S	0007346	biological_process	regulation of mitotic cell cycle
S	0016301	molecular_function	kinase activity
S	0016740	molecular_function	transferase activity
S	0032206	biological_process	positive regulation of telomere maintenance
S	0033044	biological_process	regulation of chromosome organization
S	0035865	biological_process	cellular response to potassium ion
S	0043549	biological_process	regulation of kinase activity
S	0046872	molecular_function	metal ion binding
S	0051225	biological_process	spindle assembly
S	0106310	molecular_function	protein serine kinase activity
S	0140677	molecular_function	molecular function activator activity
S	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly
T	0000166	molecular_function	nucleotide binding
T	0000922	cellular_component	spindle pole
T	0004672	molecular_function	protein kinase activity
T	0004674	molecular_function	protein serine/threonine kinase activity
T	0005515	molecular_function	protein binding
T	0005524	molecular_function	ATP binding
T	0005634	cellular_component	nucleus
T	0005654	cellular_component	nucleoplasm
T	0005737	cellular_component	cytoplasm
T	0005813	cellular_component	centrosome
T	0005815	cellular_component	microtubule organizing center
T	0005874	cellular_component	microtubule
T	0006468	biological_process	protein phosphorylation
T	0007346	biological_process	regulation of mitotic cell cycle
T	0016301	molecular_function	kinase activity
T	0016740	molecular_function	transferase activity
T	0032206	biological_process	positive regulation of telomere maintenance
T	0033044	biological_process	regulation of chromosome organization
T	0035865	biological_process	cellular response to potassium ion
T	0043549	biological_process	regulation of kinase activity
T	0046872	molecular_function	metal ion binding
T	0051225	biological_process	spindle assembly
T	0106310	molecular_function	protein serine kinase activity
T	0140677	molecular_function	molecular function activator activity
T	1900227	biological_process	positive regulation of NLRP3 inflammasome complex assembly

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGRGQFSEVYrAaclldgvp..........VALK

Chain	Residue	Details
K	ILE40-LYS63

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VmHrDIKpaNVFI

Chain	Residue	Details
K	VAL157-ILE169

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	700
Details	Domain: {"description":"FISNA","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	3160
Details	Domain: {"description":"NACHT","evidences":[{"source":"PROSITE-ProRule","id":"PRU00136","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	200
Details	Repeat: {"description":"LRR 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	210
Details	Repeat: {"description":"LRR 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	200
Details	Repeat: {"description":"LRR 3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	210
Details	Repeat: {"description":"LRR 4"}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	200
Details	Repeat: {"description":"LRR 5"}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	210
Details	Repeat: {"description":"LRR 6"}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	200
Details	Repeat: {"description":"LRR 7"}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	210
Details	Repeat: {"description":"LRR 8"}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	210
Details	Repeat: {"description":"LRR 9"}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	40
Details	Motif: {"description":"KFERQ-like motif 1","evidences":[{"source":"PubMed","id":"36586411","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	40
Details	Motif: {"description":"KFERQ-like motif 2","evidences":[{"source":"PubMed","id":"36586411","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	40
Details	Motif: {"description":"KFERQ-like motif 3","evidences":[{"source":"PubMed","id":"36586411","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	40
Details	Motif: {"description":"KFERQ-like motif 4","evidences":[{"source":"PubMed","id":"36586411","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"35254907","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36142182","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36442502","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7VTP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7ZGU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8EJ4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	80
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00136","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"17483456","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34687713","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35114687","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35254907","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36142182","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36442502","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7ALV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7PZC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7VTP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7ZGU","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"8EJ4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"PubMed","id":"34687713","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35114687","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35254907","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"36142182","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7ALV","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7PZC","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7VTP","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"7ZGU","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	40
Details	Modified residue: {"description":"Phosphotyrosine; by BTK","evidences":[{"source":"PubMed","id":"34554188","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	20
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"Q8R4B8","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	40
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"28943315","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI22
Number of Residues	10
Details	Modified residue: {"description":"Phosphoserine; by MAPK8","evidences":[{"source":"PubMed","id":"35114687","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI23
Number of Residues	10
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"35114687","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI24
Number of Residues	10
Details	Modified residue: {"description":"Phosphoserine; by LATS1 and LATS2","evidences":[{"source":"PubMed","id":"39173637","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI25
Number of Residues	20
Details	Modified residue: {"description":"Phosphoserine; by CSNK1A1","evidences":[{"source":"PubMed","id":"34615873","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI26
Number of Residues	10
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"27043286","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI27
Number of Residues	30
Details	Lipidation: {"description":"S-palmitoyl cysteine","evidences":[{"source":"PubMed","id":"36586411","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI28
Number of Residues	10
Details	Lipidation: {"description":"S-palmitoyl cysteine","evidences":[{"source":"PubMed","id":"39173637","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI29
Number of Residues	20
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"37575012","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI30
Number of Residues	40
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"34615873","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI31
Number of Residues	130
Details	Region: {"description":"NTE motif","evidences":[{"source":"PubMed","id":"19941817","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI32
Number of Residues	10
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI33
Number of Residues	90
Details	Binding site: {"evidences":[{"source":"PubMed","id":"19941817","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"2WQN","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI34
Number of Residues	10
Details	Site: {"description":"Autoinhibitory","evidences":[{"source":"PubMed","id":"19941817","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---