8CGV

Tiamulin bound to the 50S subunit

Functional Information from GO Data

Chain	GOid	namespace	contents
0	0000027	biological_process	ribosomal large subunit assembly
0	0000049	molecular_function	tRNA binding
0	0002181	biological_process	cytoplasmic translation
0	0003735	molecular_function	structural constituent of ribosome
0	0005737	cellular_component	cytoplasm
0	0005829	cellular_component	cytosol
0	0005840	cellular_component	ribosome
0	0006412	biological_process	translation
0	0022625	cellular_component	cytosolic large ribosomal subunit
0	0046677	biological_process	response to antibiotic
0	1990904	cellular_component	ribonucleoprotein complex
1	0002181	biological_process	cytoplasmic translation
1	0003735	molecular_function	structural constituent of ribosome
1	0005515	molecular_function	protein binding
1	0005737	cellular_component	cytoplasm
1	0005840	cellular_component	ribosome
1	0006412	biological_process	translation
1	0022625	cellular_component	cytosolic large ribosomal subunit
1	1990904	cellular_component	ribonucleoprotein complex
2	0002181	biological_process	cytoplasmic translation
2	0003735	molecular_function	structural constituent of ribosome
2	0005737	cellular_component	cytoplasm
2	0005840	cellular_component	ribosome
2	0006412	biological_process	translation
2	0022625	cellular_component	cytosolic large ribosomal subunit
2	1990904	cellular_component	ribonucleoprotein complex
3	0002181	biological_process	cytoplasmic translation
3	0003735	molecular_function	structural constituent of ribosome
3	0005737	cellular_component	cytoplasm
3	0005840	cellular_component	ribosome
3	0006412	biological_process	translation
3	0022625	cellular_component	cytosolic large ribosomal subunit
3	1990904	cellular_component	ribonucleoprotein complex
c	0000027	biological_process	ribosomal large subunit assembly
c	0002181	biological_process	cytoplasmic translation
c	0003723	molecular_function	RNA binding
c	0003735	molecular_function	structural constituent of ribosome
c	0005515	molecular_function	protein binding
c	0005737	cellular_component	cytoplasm
c	0005829	cellular_component	cytosol
c	0005840	cellular_component	ribosome
c	0006412	biological_process	translation
c	0008270	molecular_function	zinc ion binding
c	0015934	cellular_component	large ribosomal subunit
c	0016740	molecular_function	transferase activity
c	0019843	molecular_function	rRNA binding
c	0022625	cellular_component	cytosolic large ribosomal subunit
c	0032297	biological_process	negative regulation of DNA-templated DNA replication initiation
c	1990082	cellular_component	DnaA-L2 complex
c	1990904	cellular_component	ribonucleoprotein complex
d	0000027	biological_process	ribosomal large subunit assembly
d	0002181	biological_process	cytoplasmic translation
d	0003735	molecular_function	structural constituent of ribosome
d	0005515	molecular_function	protein binding
d	0005737	cellular_component	cytoplasm
d	0005829	cellular_component	cytosol
d	0005840	cellular_component	ribosome
d	0006412	biological_process	translation
d	0019843	molecular_function	rRNA binding
d	0022625	cellular_component	cytosolic large ribosomal subunit
d	1990904	cellular_component	ribonucleoprotein complex
e	0001070	molecular_function	RNA-binding transcription regulator activity
e	0002181	biological_process	cytoplasmic translation
e	0003677	molecular_function	DNA binding
e	0003735	molecular_function	structural constituent of ribosome
e	0005515	molecular_function	protein binding
e	0005737	cellular_component	cytoplasm
e	0005829	cellular_component	cytosol
e	0005840	cellular_component	ribosome
e	0006353	biological_process	DNA-templated transcription termination
e	0006412	biological_process	translation
e	0006417	biological_process	regulation of translation
e	0015934	cellular_component	large ribosomal subunit
e	0017148	biological_process	negative regulation of translation
e	0019843	molecular_function	rRNA binding
e	0022625	cellular_component	cytosolic large ribosomal subunit
e	0030371	molecular_function	translation repressor activity
e	0031555	biological_process	transcriptional attenuation
e	0042255	biological_process	ribosome assembly
e	0045892	biological_process	negative regulation of DNA-templated transcription
e	0046677	biological_process	response to antibiotic
e	0048027	molecular_function	mRNA 5'-UTR binding
e	0060698	molecular_function	endoribonuclease inhibitor activity
e	1990904	cellular_component	ribonucleoprotein complex
e	2000766	biological_process	negative regulation of cytoplasmic translation
g	0000027	biological_process	ribosomal large subunit assembly
g	0002181	biological_process	cytoplasmic translation
g	0003735	molecular_function	structural constituent of ribosome
g	0005737	cellular_component	cytoplasm
g	0005829	cellular_component	cytosol
g	0005840	cellular_component	ribosome
g	0006412	biological_process	translation
g	0019843	molecular_function	rRNA binding
g	0022625	cellular_component	cytosolic large ribosomal subunit
g	0046677	biological_process	response to antibiotic
g	0070180	molecular_function	large ribosomal subunit rRNA binding
g	1990904	cellular_component	ribonucleoprotein complex
h	0002181	biological_process	cytoplasmic translation
h	0003735	molecular_function	structural constituent of ribosome
h	0005515	molecular_function	protein binding
h	0005737	cellular_component	cytoplasm
h	0005829	cellular_component	cytosol
h	0005840	cellular_component	ribosome
h	0006412	biological_process	translation
h	0019843	molecular_function	rRNA binding
h	0022625	cellular_component	cytosolic large ribosomal subunit
h	0070180	molecular_function	large ribosomal subunit rRNA binding
h	1990904	cellular_component	ribonucleoprotein complex
i	0002181	biological_process	cytoplasmic translation
i	0003729	molecular_function	mRNA binding
i	0003735	molecular_function	structural constituent of ribosome
i	0005737	cellular_component	cytoplasm
i	0005829	cellular_component	cytosol
i	0005840	cellular_component	ribosome
i	0006412	biological_process	translation
i	0008270	molecular_function	zinc ion binding
i	0017148	biological_process	negative regulation of translation
i	0022625	cellular_component	cytosolic large ribosomal subunit
i	0048027	molecular_function	mRNA 5'-UTR binding
i	0070180	molecular_function	large ribosomal subunit rRNA binding
i	1990904	cellular_component	ribonucleoprotein complex
j	0002181	biological_process	cytoplasmic translation
j	0003735	molecular_function	structural constituent of ribosome
j	0005515	molecular_function	protein binding
j	0005737	cellular_component	cytoplasm
j	0005829	cellular_component	cytosol
j	0005840	cellular_component	ribosome
j	0006412	biological_process	translation
j	0015934	cellular_component	large ribosomal subunit
j	0019843	molecular_function	rRNA binding
j	0022625	cellular_component	cytosolic large ribosomal subunit
j	0070180	molecular_function	large ribosomal subunit rRNA binding
j	1990904	cellular_component	ribonucleoprotein complex
k	0002181	biological_process	cytoplasmic translation
k	0003735	molecular_function	structural constituent of ribosome
k	0005515	molecular_function	protein binding
k	0005737	cellular_component	cytoplasm
k	0005840	cellular_component	ribosome
k	0006412	biological_process	translation
k	0015934	cellular_component	large ribosomal subunit
k	0019843	molecular_function	rRNA binding
k	0022625	cellular_component	cytosolic large ribosomal subunit
k	1990904	cellular_component	ribonucleoprotein complex
l	0000027	biological_process	ribosomal large subunit assembly
l	0000049	molecular_function	tRNA binding
l	0002181	biological_process	cytoplasmic translation
l	0003735	molecular_function	structural constituent of ribosome
l	0005737	cellular_component	cytoplasm
l	0005840	cellular_component	ribosome
l	0006412	biological_process	translation
l	0019843	molecular_function	rRNA binding
l	0022625	cellular_component	cytosolic large ribosomal subunit
l	1990904	cellular_component	ribonucleoprotein complex
m	0000027	biological_process	ribosomal large subunit assembly
m	0002181	biological_process	cytoplasmic translation
m	0003735	molecular_function	structural constituent of ribosome
m	0005515	molecular_function	protein binding
m	0005737	cellular_component	cytoplasm
m	0005829	cellular_component	cytosol
m	0005840	cellular_component	ribosome
m	0006412	biological_process	translation
m	0022625	cellular_component	cytosolic large ribosomal subunit
m	1990904	cellular_component	ribonucleoprotein complex
n	0002181	biological_process	cytoplasmic translation
n	0003735	molecular_function	structural constituent of ribosome
n	0005737	cellular_component	cytoplasm
n	0005829	cellular_component	cytosol
n	0005840	cellular_component	ribosome
n	0006412	biological_process	translation
n	0008097	molecular_function	5S rRNA binding
n	0019843	molecular_function	rRNA binding
n	0022625	cellular_component	cytosolic large ribosomal subunit
n	1990904	cellular_component	ribonucleoprotein complex
o	0000027	biological_process	ribosomal large subunit assembly
o	0002181	biological_process	cytoplasmic translation
o	0003735	molecular_function	structural constituent of ribosome
o	0005737	cellular_component	cytoplasm
o	0005829	cellular_component	cytosol
o	0005840	cellular_component	ribosome
o	0006412	biological_process	translation
o	0019843	molecular_function	rRNA binding
o	0022625	cellular_component	cytosolic large ribosomal subunit
o	0070180	molecular_function	large ribosomal subunit rRNA binding
o	1990904	cellular_component	ribonucleoprotein complex
p	0000027	biological_process	ribosomal large subunit assembly
p	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
p	0002181	biological_process	cytoplasmic translation
p	0003729	molecular_function	mRNA binding
p	0003735	molecular_function	structural constituent of ribosome
p	0005737	cellular_component	cytoplasm
p	0005829	cellular_component	cytosol
p	0005840	cellular_component	ribosome
p	0006412	biological_process	translation
p	0017148	biological_process	negative regulation of translation
p	0019843	molecular_function	rRNA binding
p	0022625	cellular_component	cytosolic large ribosomal subunit
p	0070180	molecular_function	large ribosomal subunit rRNA binding
p	1990904	cellular_component	ribonucleoprotein complex
q	0002181	biological_process	cytoplasmic translation
q	0003723	molecular_function	RNA binding
q	0003735	molecular_function	structural constituent of ribosome
q	0005737	cellular_component	cytoplasm
q	0005829	cellular_component	cytosol
q	0005840	cellular_component	ribosome
q	0006412	biological_process	translation
q	0019843	molecular_function	rRNA binding
q	0022625	cellular_component	cytosolic large ribosomal subunit
q	1990904	cellular_component	ribonucleoprotein complex
r	0002181	biological_process	cytoplasmic translation
r	0003735	molecular_function	structural constituent of ribosome
r	0005515	molecular_function	protein binding
r	0005737	cellular_component	cytoplasm
r	0005829	cellular_component	cytosol
r	0005840	cellular_component	ribosome
r	0006412	biological_process	translation
r	0015934	cellular_component	large ribosomal subunit
r	0019843	molecular_function	rRNA binding
r	0022625	cellular_component	cytosolic large ribosomal subunit
r	0042255	biological_process	ribosome assembly
r	0042256	biological_process	cytosolic ribosome assembly
r	0046677	biological_process	response to antibiotic
r	0070180	molecular_function	large ribosomal subunit rRNA binding
r	1902626	biological_process	assembly of large subunit precursor of preribosome
r	1990904	cellular_component	ribonucleoprotein complex
s	0000027	biological_process	ribosomal large subunit assembly
s	0002181	biological_process	cytoplasmic translation
s	0003735	molecular_function	structural constituent of ribosome
s	0005515	molecular_function	protein binding
s	0005737	cellular_component	cytoplasm
s	0005840	cellular_component	ribosome
s	0006412	biological_process	translation
s	0019843	molecular_function	rRNA binding
s	0022625	cellular_component	cytosolic large ribosomal subunit
s	1990904	cellular_component	ribonucleoprotein complex
t	0000027	biological_process	ribosomal large subunit assembly
t	0002181	biological_process	cytoplasmic translation
t	0003723	molecular_function	RNA binding
t	0003735	molecular_function	structural constituent of ribosome
t	0005515	molecular_function	protein binding
t	0005737	cellular_component	cytoplasm
t	0005829	cellular_component	cytosol
t	0005840	cellular_component	ribosome
t	0006412	biological_process	translation
t	0019843	molecular_function	rRNA binding
t	0022625	cellular_component	cytosolic large ribosomal subunit
t	0070180	molecular_function	large ribosomal subunit rRNA binding
t	1990904	cellular_component	ribonucleoprotein complex
u	0000027	biological_process	ribosomal large subunit assembly
u	0002181	biological_process	cytoplasmic translation
u	0003723	molecular_function	RNA binding
u	0003735	molecular_function	structural constituent of ribosome
u	0005737	cellular_component	cytoplasm
u	0005829	cellular_component	cytosol
u	0005840	cellular_component	ribosome
u	0006412	biological_process	translation
u	0008097	molecular_function	5S rRNA binding
u	0009314	biological_process	response to radiation
u	0017148	biological_process	negative regulation of translation
u	0019843	molecular_function	rRNA binding
u	0022625	cellular_component	cytosolic large ribosomal subunit
u	1990904	cellular_component	ribonucleoprotein complex
v	0000027	biological_process	ribosomal large subunit assembly
v	0000049	molecular_function	tRNA binding
v	0001558	biological_process	regulation of cell growth
v	0002181	biological_process	cytoplasmic translation
v	0003735	molecular_function	structural constituent of ribosome
v	0005515	molecular_function	protein binding
v	0005737	cellular_component	cytoplasm
v	0005840	cellular_component	ribosome
v	0006412	biological_process	translation
v	0019843	molecular_function	rRNA binding
v	0022625	cellular_component	cytosolic large ribosomal subunit
v	0042256	biological_process	cytosolic ribosome assembly
v	0043022	molecular_function	ribosome binding
v	0090070	biological_process	positive regulation of ribosome biogenesis
v	1902626	biological_process	assembly of large subunit precursor of preribosome
v	1990904	cellular_component	ribonucleoprotein complex
w	0000027	biological_process	ribosomal large subunit assembly
w	0002181	biological_process	cytoplasmic translation
w	0003735	molecular_function	structural constituent of ribosome
w	0005515	molecular_function	protein binding
w	0005737	cellular_component	cytoplasm
w	0005829	cellular_component	cytosol
w	0005840	cellular_component	ribosome
w	0006412	biological_process	translation
w	0019843	molecular_function	rRNA binding
w	0022625	cellular_component	cytosolic large ribosomal subunit
w	1990904	cellular_component	ribonucleoprotein complex
x	0000027	biological_process	ribosomal large subunit assembly
x	0002181	biological_process	cytoplasmic translation
x	0003735	molecular_function	structural constituent of ribosome
x	0005737	cellular_component	cytoplasm
x	0005840	cellular_component	ribosome
x	0006412	biological_process	translation
x	0019843	molecular_function	rRNA binding
x	0022625	cellular_component	cytosolic large ribosomal subunit
x	1990904	cellular_component	ribonucleoprotein complex
y	0000027	biological_process	ribosomal large subunit assembly
y	0002181	biological_process	cytoplasmic translation
y	0003735	molecular_function	structural constituent of ribosome
y	0005737	cellular_component	cytoplasm
y	0005840	cellular_component	ribosome
y	0006412	biological_process	translation
y	0015934	cellular_component	large ribosomal subunit
y	0022625	cellular_component	cytosolic large ribosomal subunit
y	1990904	cellular_component	ribonucleoprotein complex
z	0000027	biological_process	ribosomal large subunit assembly
z	0000302	biological_process	response to reactive oxygen species
z	0002181	biological_process	cytoplasmic translation
z	0003735	molecular_function	structural constituent of ribosome
z	0005515	molecular_function	protein binding
z	0005737	cellular_component	cytoplasm
z	0005829	cellular_component	cytosol
z	0005840	cellular_component	ribosome
z	0006412	biological_process	translation
z	0009314	biological_process	response to radiation
z	0015934	cellular_component	large ribosomal subunit
z	0022625	cellular_component	cytosolic large ribosomal subunit
z	1990904	cellular_component	ribonucleoprotein complex

Functional Information from PROSITE/UniProt

site_id	PS00049
Number of Residues	27
Details	RIBOSOMAL_L14 Ribosomal protein L14 signature. AVVVrtkkgvrrp.DGsvirFdgNacVL

Chain	Residue	Details
j	ALA60-LEU86

---

site_id	PS00050
Number of Residues	16
Details	RIBOSOMAL_L23 Ribosomal protein L23 signature. KKAYVTLKegqnldfV

Chain	Residue	Details
s	LYS81-VAL96

---

site_id	PS00430
Number of Residues	66
Details	TONB_DEPENDENT_REC_1 TonB-dependent receptor (TBDR) proteins signature 1. mireerllkvlraphvsekastameksntivlkvakdatkaeikaavqklfevevevv.........................................................NTLVVKGK

Chain	Residue	Details
s	MET1-LYS66

---

site_id	PS00464
Number of Residues	25
Details	RIBOSOMAL_L22 Ribosomal protein L22 signature. KriMpRAkGRadrilkrtSHITVvV

Chain	Residue	Details
r	LYS83-VAL107

---

site_id	PS00467
Number of Residues	12
Details	RIBOSOMAL_L2 Ribosomal protein L2 signature. PtvRGTAmNPvD

Chain	Residue	Details
c	PRO218-ASP229

---

site_id	PS00474
Number of Residues	24
Details	RIBOSOMAL_L3 Ribosomal protein L3 signature. FadvkkvDvtGtSkGKGfaGtvkR

Chain	Residue	Details
d	PHE101-ARG124

---

site_id	PS00475
Number of Residues	31
Details	RIBOSOMAL_L15 Ribosomal protein L15 signature. KVILaGeVttp..VtVrglrVTkgAraaIeaaG

Chain	Residue	Details
k	LYS109-GLY139

---

site_id	PS00525
Number of Residues	9
Details	RIBOSOMAL_L6_1 Ribosomal protein L6 signature 1. PEpYKGKGV

Chain	Residue	Details
g	PRO154-VAL162

---

site_id	PS00579
Number of Residues	15
Details	RIBOSOMAL_L29 Ribosomal protein L29 signature. QSHLLKqVRRDVARV

Chain	Residue	Details
x	GLN39-VAL53

---

site_id	PS00582
Number of Residues	20
Details	RIBOSOMAL_L33 Ribosomal protein L33 signature. YtTtKNkrtkPekLElkKFD

Chain	Residue	Details
0	TYR21-ASP40

---

site_id	PS00586
Number of Residues	12
Details	RIBOSOMAL_L16_1 Ribosomal protein L16 signature 1. KRqGKIWIRVFP

Chain	Residue	Details
l	LYS58-PRO69

---

site_id	PS00634
Number of Residues	33
Details	RIBOSOMAL_L30 Ribosomal protein L30 signature. TLlgLgLrRigHtVeredtpairgMInaVsfMV

Chain	Residue	Details
y	THR23-VAL55

---

site_id	PS00651
Number of Residues	28
Details	RIBOSOMAL_L9 Ribosomal protein L9 signature. GslGdqvnVkaGYarNFLvpqgkAvpaT

Chain	Residue	Details
h	GLY13-THR40

---

site_id	PS00783
Number of Residues	23
Details	RIBOSOMAL_L13 Ribosomal protein L13 signature. VKGMLPkgpl.GRamfrkLkVYaG

Chain	Residue	Details
i	VAL105-GLY127

---

site_id	PS00784
Number of Residues	20
Details	RIBOSOMAL_L34 Ribosomal protein L34 signature. KRTFQpsvlkRnrsh.GFraR

Chain	Residue	Details
1	LYS2-ARG21

---

site_id	PS00828
Number of Residues	27
Details	RIBOSOMAL_L36 Ribosomal protein L36 signature. CrnCkiVkRdgvIrViCsaepKHkQrQ

Chain	Residue	Details
3	CYS11-GLN37

---

site_id	PS00831
Number of Residues	15
Details	RIBOSOMAL_L27 Ribosomal protein L27 signature. GsIIvRQRGtkfhaG

Chain	Residue	Details
v	GLY34-GLY48

---

site_id	PS00936
Number of Residues	27
Details	RIBOSOMAL_L35 Ribosomal protein L35 signature. KTVrGAaKRFkktgkggfkhkhanl.RH

Chain	Residue	Details
2	LYS5-HIS31

---

site_id	PS00937
Number of Residues	17
Details	RIBOSOMAL_L20 Ribosomal protein L20 signature. KrqfRqLWIARINaaaR

Chain	Residue	Details
p	LYS54-ARG70

---

site_id	PS01015
Number of Residues	16
Details	RIBOSOMAL_L19 Ribosomal protein L19 signature. VkRrGAVR.KAKLYYLR

Chain	Residue	Details
o	VAL86-ARG101

---

site_id	PS01108
Number of Residues	18
Details	RIBOSOMAL_L24 Ribosomal protein L24 signature. DDeViVLtGkdKGkr.GkV

Chain	Residue	Details
t	ASP8-VAL25

---

site_id	PS01167
Number of Residues	23
Details	RIBOSOMAL_L17 Ribosomal protein L17 signature. IkTTlpKaKelrrvvEpLITlAK

Chain	Residue	Details
m	ILE34-LYS56

---

site_id	PS01169
Number of Residues	23
Details	RIBOSOMAL_L21 Ribosomal protein L21 signature. VkivKfrrRKhyrkqqGHRQwfT

Chain	Residue	Details
q	VAL72-THR94

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:34403461, ECO:0000269|PubMed:34504068, ECO:0000312|PDB:7O19, ECO:0000312|PDB:7O1A, ECO:0000312|PDB:7O1C, ECO:0000312|PDB:7OIZ, ECO:0000312|PDB:7OJ0

Chain	Residue	Details
r	LYS90
h	LYS83
h	LYS112

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: (3R)-3-hydroxyarginine => ECO:0000269|PubMed:23103944

Chain	Residue	Details
l	4D481

---