7Z8B

Structure of CRL7FBXW8 reveals coupling with CUL1-RBX1/ROC1 for multi-cullin-RING E3-catalyzed ubiquitin ligation

Functional Information from GO Data

Chain	GOid	namespace	contents
C	0000226	biological_process	microtubule cytoskeleton organization
C	0000281	biological_process	mitotic cytokinesis
C	0001570	biological_process	vasculogenesis
C	0001837	biological_process	epithelial to mesenchymal transition
C	0001890	biological_process	placenta development
C	0005515	molecular_function	protein binding
C	0005680	cellular_component	anaphase-promoting complex
C	0005737	cellular_component	cytoplasm
C	0005794	cellular_component	Golgi apparatus
C	0005813	cellular_component	centrosome
C	0005829	cellular_component	cytosol
C	0005856	cellular_component	cytoskeleton
C	0006508	biological_process	proteolysis
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0007030	biological_process	Golgi organization
C	0007088	biological_process	regulation of mitotic nuclear division
C	0008286	biological_process	insulin receptor signaling pathway
C	0016567	biological_process	protein ubiquitination
C	0031461	cellular_component	cullin-RING ubiquitin ligase complex
C	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0046627	biological_process	negative regulation of insulin receptor signaling pathway
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0050775	biological_process	positive regulation of dendrite morphogenesis
C	0160072	molecular_function	ubiquitin ligase complex scaffold activity
C	1990393	cellular_component	3M complex
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0005794	cellular_component	Golgi apparatus
F	0005814	cellular_component	centriole
F	0005829	cellular_component	cytosol
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0007030	biological_process	Golgi organization
F	0008283	biological_process	cell population proliferation
F	0008286	biological_process	insulin receptor signaling pathway
F	0016567	biological_process	protein ubiquitination
F	0019005	cellular_component	SCF ubiquitin ligase complex
F	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
F	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
F	0036064	cellular_component	ciliary basal body
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0045727	biological_process	positive regulation of translation
F	0046627	biological_process	negative regulation of insulin receptor signaling pathway
F	0048471	cellular_component	perinuclear region of cytoplasm
F	0050775	biological_process	positive regulation of dendrite morphogenesis
F	0060271	biological_process	cilium assembly
F	0060716	biological_process	labyrinthine layer blood vessel development
F	1990393	cellular_component	3M complex
F	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
F	2000059	biological_process	negative regulation of ubiquitin-dependent protein catabolic process
R	0000045	biological_process	autophagosome assembly
R	0000082	biological_process	G1/S transition of mitotic cell cycle
R	0000165	biological_process	MAPK cascade
R	0000209	biological_process	protein polyubiquitination
R	0000423	biological_process	mitophagy
R	0001837	biological_process	epithelial to mesenchymal transition
R	0004842	molecular_function	ubiquitin-protein transferase activity
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005654	cellular_component	nucleoplasm
R	0005737	cellular_component	cytoplasm
R	0005829	cellular_component	cytosol
R	0006281	biological_process	DNA repair
R	0006283	biological_process	transcription-coupled nucleotide-excision repair
R	0006366	biological_process	transcription by RNA polymerase II
R	0006368	biological_process	transcription elongation by RNA polymerase II
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0006513	biological_process	protein monoubiquitination
R	0006915	biological_process	apoptotic process
R	0006974	biological_process	DNA damage response
R	0006979	biological_process	response to oxidative stress
R	0007040	biological_process	lysosome organization
R	0007283	biological_process	spermatogenesis
R	0007346	biological_process	regulation of mitotic cell cycle
R	0008270	molecular_function	zinc ion binding
R	0008286	biological_process	insulin receptor signaling pathway
R	0010507	biological_process	negative regulation of autophagy
R	0010508	biological_process	positive regulation of autophagy
R	0016567	biological_process	protein ubiquitination
R	0016740	molecular_function	transferase activity
R	0019005	cellular_component	SCF ubiquitin ligase complex
R	0019221	biological_process	cytokine-mediated signaling pathway
R	0019788	molecular_function	NEDD8 transferase activity
R	0030163	biological_process	protein catabolic process
R	0030330	biological_process	DNA damage response, signal transduction by p53 class mediator
R	0030891	cellular_component	VCB complex
R	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
R	0031461	cellular_component	cullin-RING ubiquitin ligase complex
R	0031462	cellular_component	Cul2-RING ubiquitin ligase complex
R	0031463	cellular_component	Cul3-RING ubiquitin ligase complex
R	0031464	cellular_component	Cul4A-RING E3 ubiquitin ligase complex
R	0031465	cellular_component	Cul4B-RING E3 ubiquitin ligase complex
R	0031466	cellular_component	Cul5-RING ubiquitin ligase complex
R	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
R	0031625	molecular_function	ubiquitin protein ligase binding
R	0031669	biological_process	cellular response to nutrient levels
R	0032435	biological_process	negative regulation of proteasomal ubiquitin-dependent protein catabolic process
R	0032436	biological_process	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
R	0032480	biological_process	negative regulation of type I interferon production
R	0032481	biological_process	positive regulation of type I interferon production
R	0032869	biological_process	cellular response to insulin stimulus
R	0034198	biological_process	cellular response to amino acid starvation
R	0034450	molecular_function	ubiquitin-ubiquitin ligase activity
R	0034599	biological_process	cellular response to oxidative stress
R	0034644	biological_process	cellular response to UV
R	0035279	biological_process	miRNA-mediated gene silencing by mRNA destabilization
R	0038066	biological_process	p38MAPK cascade
R	0038202	biological_process	TORC1 signaling
R	0042110	biological_process	T cell activation
R	0042770	biological_process	signal transduction in response to DNA damage
R	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
R	0043124	biological_process	negative regulation of canonical NF-kappaB signal transduction
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0043687	biological_process	post-translational protein modification
R	0044877	molecular_function	protein-containing complex binding
R	0045116	biological_process	protein neddylation
R	0045727	biological_process	positive regulation of translation
R	0045732	biological_process	positive regulation of protein catabolic process
R	0045944	biological_process	positive regulation of transcription by RNA polymerase II
R	0046627	biological_process	negative regulation of insulin receptor signaling pathway
R	0046872	molecular_function	metal ion binding
R	0060090	molecular_function	molecular adaptor activity
R	0060337	biological_process	type I interferon-mediated signaling pathway
R	0061629	molecular_function	RNA polymerase II-specific DNA-binding transcription factor binding
R	0061630	molecular_function	ubiquitin protein ligase activity
R	0061663	molecular_function	NEDD8 ligase activity
R	0062197	biological_process	cellular response to chemical stress
R	0070294	biological_process	renal sodium ion absorption
R	0070936	biological_process	protein K48-linked ubiquitination
R	0071230	biological_process	cellular response to amino acid stimulus
R	0080008	cellular_component	Cul4-RING E3 ubiquitin ligase complex
R	0090090	biological_process	negative regulation of canonical Wnt signaling pathway
R	0090734	cellular_component	site of DNA damage
R	0097510	biological_process	base-excision repair, AP site formation via deaminated base removal
R	0097602	molecular_function	cullin family protein binding
R	0140627	biological_process	ubiquitin-dependent protein catabolic process via the C-end degron rule pathway
R	0160240	biological_process	RNA polymerase II transcription initiation surveillance
R	1900076	biological_process	regulation of cellular response to insulin stimulus
R	1901525	biological_process	negative regulation of mitophagy
R	1901797	biological_process	negative regulation of signal transduction by p53 class mediator
R	1902499	biological_process	positive regulation of protein autoubiquitination
R	1902883	biological_process	negative regulation of response to oxidative stress
R	1904037	biological_process	positive regulation of epithelial cell apoptotic process
R	1904262	biological_process	negative regulation of TORC1 signaling
R	1904263	biological_process	positive regulation of TORC1 signaling
R	2000059	biological_process	negative regulation of ubiquitin-dependent protein catabolic process
S	0000209	biological_process	protein polyubiquitination
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005654	cellular_component	nucleoplasm
S	0005737	cellular_component	cytoplasm
S	0005813	cellular_component	centrosome
S	0005829	cellular_component	cytosol
S	0006338	biological_process	chromatin remodeling
S	0006511	biological_process	ubiquitin-dependent protein catabolic process
S	0006513	biological_process	protein monoubiquitination
S	0006915	biological_process	apoptotic process
S	0008013	molecular_function	beta-catenin binding
S	0016567	biological_process	protein ubiquitination
S	0019005	cellular_component	SCF ubiquitin ligase complex
S	0019904	molecular_function	protein domain specific binding
S	0031146	biological_process	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
S	0031467	cellular_component	Cul7-RING ubiquitin ligase complex
S	0031519	cellular_component	PcG protein complex
S	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
S	0051457	biological_process	maintenance of protein location in nucleus
S	0061564	biological_process	axon development
S	0070936	biological_process	protein K48-linked ubiquitination
S	0097602	molecular_function	cullin family protein binding
S	0140677	molecular_function	molecular function activator activity
S	0160072	molecular_function	ubiquitin ligase complex scaffold activity
S	1904037	biological_process	positive regulation of epithelial cell apoptotic process
S	1990444	molecular_function	F-box domain binding
S	1990756	molecular_function	ubiquitin-like ligase-substrate adaptor activity
S	1990757	molecular_function	ubiquitin ligase activator activity

Functional Information from PROSITE/UniProt

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. AVAAyeDgFLNIWDL

Chain	Residue	Details
F	ALA272-LEU286

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692

Chain	Residue	Details
S	THR131

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site_id	SWS_FT_FI2
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) => ECO:0007744|PubMed:25114211

Chain	Residue	Details
S	LYS142
R	CYS45
R	CYS75
R	HIS77
R	HIS80
R	CYS94
R	ASP97

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site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:11961546, ECO:0000269|PubMed:38605244, ECO:0007744|PDB:1LDJ, ECO:0007744|PDB:1LDK, ECO:0007744|PDB:1U6G, ECO:0007744|PDB:2HYE, ECO:0007744|PDB:3DPL, ECO:0007744|PDB:3DQV, ECO:0007744|PDB:3RTR, ECO:0007744|PDB:4F52, ECO:0007744|PDB:4P5O, ECO:0007744|PDB:7Z8B, ECO:0007744|PDB:8Q7H, ECO:0007744|PDB:8RHZ

Chain	Residue	Details
R	CYS53
R	CYS56
R	CYS68
R	HIS82

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site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: BINDING => ECO:0000269|PubMed:11961546, ECO:0000269|PubMed:38605244, ECO:0007744|PDB:1LDJ, ECO:0007744|PDB:1LDK, ECO:0007744|PDB:1U6G, ECO:0007744|PDB:2HYE, ECO:0007744|PDB:3DQV, ECO:0007744|PDB:3RTR, ECO:0007744|PDB:4F52, ECO:0007744|PDB:4P5O, ECO:0007744|PDB:8Q7H, ECO:0007744|PDB:8RHZ

Chain	Residue	Details
R	CYS83

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site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
R	MET1

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site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: N-acetylalanine; in E3 ubiquitin-protein ligase RBX1, N-terminally processed => ECO:0000269|Ref.8, ECO:0007744|PubMed:19413330, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378

Chain	Residue	Details
R	ALA2

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site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
R	THR9

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