7YQ8
Cryo-EM structure of human topoisomerase II beta in complex with DNA and etoposide
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0000712 | biological_process | resolution of meiotic recombination intermediates |
| A | 0000792 | cellular_component | heterochromatin |
| A | 0000819 | biological_process | sister chromatid segregation |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003682 | molecular_function | chromatin binding |
| A | 0003916 | molecular_function | DNA topoisomerase activity |
| A | 0003918 | molecular_function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005730 | cellular_component | nucleolus |
| A | 0005829 | cellular_component | cytosol |
| A | 0006259 | biological_process | DNA metabolic process |
| A | 0006265 | biological_process | DNA topological change |
| A | 0016853 | molecular_function | isomerase activity |
| A | 0030183 | biological_process | B cell differentiation |
| A | 0043021 | molecular_function | ribonucleoprotein complex binding |
| A | 0045870 | biological_process | positive regulation of single stranded viral RNA replication via double stranded DNA intermediate |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0070301 | biological_process | cellular response to hydrogen peroxide |
| A | 0071318 | biological_process | cellular response to ATP |
| A | 0090398 | biological_process | cellular senescence |
| A | 1990904 | cellular_component | ribonucleoprotein complex |
| A | 2001034 | biological_process | positive regulation of double-strand break repair via nonhomologous end joining |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000712 | biological_process | resolution of meiotic recombination intermediates |
| B | 0000792 | cellular_component | heterochromatin |
| B | 0000819 | biological_process | sister chromatid segregation |
| B | 0003677 | molecular_function | DNA binding |
| B | 0003682 | molecular_function | chromatin binding |
| B | 0003916 | molecular_function | DNA topoisomerase activity |
| B | 0003918 | molecular_function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005730 | cellular_component | nucleolus |
| B | 0005829 | cellular_component | cytosol |
| B | 0006259 | biological_process | DNA metabolic process |
| B | 0006265 | biological_process | DNA topological change |
| B | 0016853 | molecular_function | isomerase activity |
| B | 0030183 | biological_process | B cell differentiation |
| B | 0043021 | molecular_function | ribonucleoprotein complex binding |
| B | 0045870 | biological_process | positive regulation of single stranded viral RNA replication via double stranded DNA intermediate |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0070301 | biological_process | cellular response to hydrogen peroxide |
| B | 0071318 | biological_process | cellular response to ATP |
| B | 0090398 | biological_process | cellular senescence |
| B | 1990904 | cellular_component | ribonucleoprotein complex |
| B | 2001034 | biological_process | positive regulation of double-strand break repair via nonhomologous end joining |
Functional Information from PROSITE/UniProt
| site_id | PS00107 |
| Number of Residues | 22 |
| Details | PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. VGGGNGALTWvTlfdqnn............AAKK |
| Chain | Residue | Details |
| A | VAL13-LYS34 |
| site_id | PS00177 |
| Number of Residues | 9 |
| Details | TOPOISOMERASE_II DNA topoisomerase II signature. LTEGDSAKS |
| Chain | Residue | Details |
| A | LEU480-SER488 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 234 |
| Details | Domain: {"description":"Toprim","evidences":[{"source":"PROSITE-ProRule","id":"PRU00995","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 18 |
| Details | Region: {"description":"Interaction with DNA"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 20 |
| Details | Motif: {"description":"Nuclear export signal"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Active site: {"description":"O-(5'-phospho-DNA)-tyrosine intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU01384","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"21778401","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00995","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00995","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"21778401","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 12 |
| Details | Site: {"description":"Interaction with DNA"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | Site: {"description":"Interaction with DNA","evidences":[{"source":"PROSITE-ProRule","id":"PRU00995","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | Site: {"description":"Transition state stabilizer"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | Site: {"description":"Important for DNA bending; intercalates between base pairs of target DNA"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 14 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI12 |
| Number of Residues | 4 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"25772364","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
