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7WTD

Cryo-EM structure of human pyruvate carboxylase with acetyl-CoA in the intermediate state 1

Functional Information from GO Data
ChainGOidnamespacecontents
A0003824molecular_functioncatalytic activity
A0004736molecular_functionpyruvate carboxylase activity
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0005737cellular_componentcytoplasm
A0005739cellular_componentmitochondrion
A0005759cellular_componentmitochondrial matrix
A0005829cellular_componentcytosol
A0006090biological_processpyruvate metabolic process
A0006094biological_processgluconeogenesis
A0006629biological_processlipid metabolic process
A0006734biological_processNADH metabolic process
A0006739biological_processNADP metabolic process
A0009374molecular_functionbiotin binding
A0010629biological_processnegative regulation of gene expression
A0016874molecular_functionligase activity
A0019074biological_processviral RNA genome packaging
A0019076biological_processviral release from host cell
A0042802molecular_functionidentical protein binding
A0044281biological_processsmall molecule metabolic process
A0044794biological_processpositive regulation by host of viral process
A0046872molecular_functionmetal ion binding
B0003824molecular_functioncatalytic activity
B0004736molecular_functionpyruvate carboxylase activity
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005759cellular_componentmitochondrial matrix
B0005829cellular_componentcytosol
B0006090biological_processpyruvate metabolic process
B0006094biological_processgluconeogenesis
B0006629biological_processlipid metabolic process
B0006734biological_processNADH metabolic process
B0006739biological_processNADP metabolic process
B0009374molecular_functionbiotin binding
B0010629biological_processnegative regulation of gene expression
B0016874molecular_functionligase activity
B0019074biological_processviral RNA genome packaging
B0019076biological_processviral release from host cell
B0042802molecular_functionidentical protein binding
B0044281biological_processsmall molecule metabolic process
B0044794biological_processpositive regulation by host of viral process
B0046872molecular_functionmetal ion binding
C0003824molecular_functioncatalytic activity
C0004736molecular_functionpyruvate carboxylase activity
C0005515molecular_functionprotein binding
C0005524molecular_functionATP binding
C0005737cellular_componentcytoplasm
C0005739cellular_componentmitochondrion
C0005759cellular_componentmitochondrial matrix
C0005829cellular_componentcytosol
C0006090biological_processpyruvate metabolic process
C0006094biological_processgluconeogenesis
C0006629biological_processlipid metabolic process
C0006734biological_processNADH metabolic process
C0006739biological_processNADP metabolic process
C0009374molecular_functionbiotin binding
C0010629biological_processnegative regulation of gene expression
C0016874molecular_functionligase activity
C0019074biological_processviral RNA genome packaging
C0019076biological_processviral release from host cell
C0042802molecular_functionidentical protein binding
C0044281biological_processsmall molecule metabolic process
C0044794biological_processpositive regulation by host of viral process
C0046872molecular_functionmetal ion binding
D0003824molecular_functioncatalytic activity
D0004736molecular_functionpyruvate carboxylase activity
D0005515molecular_functionprotein binding
D0005524molecular_functionATP binding
D0005737cellular_componentcytoplasm
D0005739cellular_componentmitochondrion
D0005759cellular_componentmitochondrial matrix
D0005829cellular_componentcytosol
D0006090biological_processpyruvate metabolic process
D0006094biological_processgluconeogenesis
D0006629biological_processlipid metabolic process
D0006734biological_processNADH metabolic process
D0006739biological_processNADP metabolic process
D0009374molecular_functionbiotin binding
D0010629biological_processnegative regulation of gene expression
D0016874molecular_functionligase activity
D0019074biological_processviral RNA genome packaging
D0019076biological_processviral release from host cell
D0042802molecular_functionidentical protein binding
D0044281biological_processsmall molecule metabolic process
D0044794biological_processpositive regulation by host of viral process
D0046872molecular_functionmetal ion binding
Functional Information from PROSITE/UniProt
site_idPS00188
Number of Residues18
DetailsBIOTIN Biotin-requiring enzymes attachment site. GQpLcvLsAMKMetvVtS
ChainResidueDetails
AGLY1134-SER1151

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsACT_SITE: ACT_SITE => ECO:0000250
ChainResidueDetails
AARG328
BARG328
CARG328
DARG328

site_idSWS_FT_FI2
Number of Residues12
DetailsBINDING: BINDING => ECO:0000250
ChainResidueDetails
ALYS152
DLYS152
DGLU236
DHIS271
AGLU236
AHIS271
BLYS152
BGLU236
BHIS271
CLYS152
CGLU236
CHIS271

site_idSWS_FT_FI3
Number of Residues12
DetailsBINDING:
ChainResidueDetails
AARG571
DARG571
DARG644
DTHR908
AARG644
ATHR908
BARG571
BARG644
BTHR908
CARG571
CARG644
CTHR908

site_idSWS_FT_FI4
Number of Residues12
DetailsBINDING: BINDING => ECO:0000269|PubMed:18297087
ChainResidueDetails
AASP572
DASP572
DHIS771
DHIS773
AHIS771
AHIS773
BASP572
BHIS771
BHIS773
CASP572
CHIS771
CHIS773

site_idSWS_FT_FI5
Number of Residues4
DetailsBINDING: via carbamate group => ECO:0000269|PubMed:18297087
ChainResidueDetails
ALYS741
BLYS741
CLYS741
DLYS741

site_idSWS_FT_FI6
Number of Residues68
DetailsMOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q05920
ChainResidueDetails
ALYS35
ALYS661
ALYS717
ALYS748
ALYS892
ALYS969
ALYS992
ALYS1061
ALYS1124
BLYS35
BLYS39
ALYS39
BLYS148
BLYS152
BLYS241
BLYS297
BLYS319
BLYS434
BLYS589
BLYS661
BLYS717
BLYS748
ALYS148
BLYS892
BLYS969
BLYS992
BLYS1061
BLYS1124
CLYS35
CLYS39
CLYS148
CLYS152
CLYS241
ALYS152
CLYS297
CLYS319
CLYS434
CLYS589
CLYS661
CLYS717
CLYS748
CLYS892
CLYS969
CLYS992
ALYS241
CLYS1061
CLYS1124
DLYS35
DLYS39
DLYS148
DLYS152
DLYS241
DLYS297
DLYS319
DLYS434
ALYS297
DLYS589
DLYS661
DLYS717
DLYS748
DLYS892
DLYS969
DLYS992
DLYS1061
DLYS1124
ALYS319
ALYS434
ALYS589

site_idSWS_FT_FI7
Number of Residues4
DetailsMOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:Q05920
ChainResidueDetails
ALYS79
BLYS79
CLYS79
DLYS79

site_idSWS_FT_FI8
Number of Residues4
DetailsMOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:Q05920
ChainResidueDetails
ALYS442
BLYS442
CLYS442
DLYS442

site_idSWS_FT_FI9
Number of Residues4
DetailsMOD_RES: N6-carboxylysine => ECO:0000269|PubMed:18297087
ChainResidueDetails
ALYS741
BLYS741
CLYS741
DLYS741

site_idSWS_FT_FI10
Number of Residues4
DetailsMOD_RES: Phosphothreonine => ECO:0000250|UniProtKB:P52873
ChainResidueDetails
ATHR1003
BTHR1003
CTHR1003
DTHR1003

site_idSWS_FT_FI11
Number of Residues4
DetailsMOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861
ChainResidueDetails
ALYS1090
BLYS1090
CLYS1090
DLYS1090

site_idSWS_FT_FI12
Number of Residues4
DetailsMOD_RES: N6-biotinyllysine => ECO:0000255|PROSITE-ProRule:PRU01066, ECO:0000269|PubMed:6548474, ECO:0000269|PubMed:7918683
ChainResidueDetails
ALYS1144
BLYS1144
CLYS1144
DLYS1144

227111

PDB entries from 2024-11-06

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