7QNA

Cryo-EM structure of human full-length alpha4beta3gamma2 GABA(A)R in complex with GABA and nanobody Nb25

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004888	molecular_function	transmembrane signaling receptor activity
A	0004890	molecular_function	GABA-A receptor activity
A	0005216	molecular_function	monoatomic ion channel activity
A	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
A	0006811	biological_process	monoatomic ion transport
A	0006821	biological_process	chloride transport
A	0007214	biological_process	gamma-aminobutyric acid signaling pathway
A	0016020	cellular_component	membrane
A	0034220	biological_process	monoatomic ion transmembrane transport
B	0004888	molecular_function	transmembrane signaling receptor activity
B	0004890	molecular_function	GABA-A receptor activity
B	0005216	molecular_function	monoatomic ion channel activity
B	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
B	0006811	biological_process	monoatomic ion transport
B	0016020	cellular_component	membrane
B	0034220	biological_process	monoatomic ion transmembrane transport
C	0004888	molecular_function	transmembrane signaling receptor activity
C	0004890	molecular_function	GABA-A receptor activity
C	0005216	molecular_function	monoatomic ion channel activity
C	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
C	0006811	biological_process	monoatomic ion transport
C	0006821	biological_process	chloride transport
C	0007214	biological_process	gamma-aminobutyric acid signaling pathway
C	0016020	cellular_component	membrane
C	0034220	biological_process	monoatomic ion transmembrane transport
D	0004888	molecular_function	transmembrane signaling receptor activity
D	0004890	molecular_function	GABA-A receptor activity
D	0005216	molecular_function	monoatomic ion channel activity
D	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
D	0006811	biological_process	monoatomic ion transport
D	0016020	cellular_component	membrane
D	0034220	biological_process	monoatomic ion transmembrane transport
E	0004888	molecular_function	transmembrane signaling receptor activity
E	0004890	molecular_function	GABA-A receptor activity
E	0005216	molecular_function	monoatomic ion channel activity
E	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
E	0006811	biological_process	monoatomic ion transport
E	0016020	cellular_component	membrane
E	0034220	biological_process	monoatomic ion transmembrane transport

Functional Information from PROSITE/UniProt

site_id	PS00236
Number of Residues	15
Details	NEUROTR_ION_CHANNEL Neurotransmitter-gated ion-channels signature. CqLqLhnFPmDehsC

Chain	Residue	Details
C	CYS151-CYS165
B	CYS136-CYS150
A	CYS172-CYS186

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	233
Details	TOPO_DOM: Extracellular => ECO:0000305

Chain	Residue	Details
C	GLN1-GLY234
B	ARG269-LYS279
D	GLN1-TYR220
D	ARG269-LYS279
E	GLN1-TYR220
E	ARG269-LYS279

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site_id	SWS_FT_FI2
Number of Residues	66
Details	TRANSMEM: Helical => ECO:0000305

Chain	Residue	Details
C	TYR235-ILE257
E	ALA246-LEU268
E	ALA280-VAL302
E	TRP426-VAL447
C	ALA261-ALA283
C	ALA295-LEU317
B	TRP426-VAL447
D	PHE221-ILE242
D	ALA246-LEU268
D	ALA280-VAL302
D	TRP426-VAL447
E	PHE221-ILE242

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site_id	SWS_FT_FI3
Number of Residues	3
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
C	ASN13
C	ASN90
C	ASN208
D	ASN303-ARG425
E	ASN243-ASP245
E	ASN303-ARG425

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site_id	SWS_FT_FI4
Number of Residues	6
Details	BINDING: BINDING => ECO:0000269|PubMed:24909990

Chain	Residue	Details
D	PHE200
E	ASP95
E	PHE200
B	ASP95
B	PHE200
D	ASP95

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site_id	SWS_FT_FI5
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:24909990, ECO:0007744|PDB:4COF

Chain	Residue	Details
B	GLU155
D	GLU155
E	GLU155

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site_id	SWS_FT_FI6
Number of Residues	9
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:24909990

Chain	Residue	Details
B	ASN8
B	ASN80
B	ASN149
D	ASN8
D	ASN80
D	ASN149
E	ASN8
E	ASN80
E	ASN149

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