7Q3Y
Structure of full-length, monomeric, soluble somatic angiotensin I-converting enzyme showing the N- and C-terminal ellipsoid domains
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001666 | biological_process | response to hypoxia |
| A | 0001822 | biological_process | kidney development |
| A | 0001974 | biological_process | blood vessel remodeling |
| A | 0002003 | biological_process | angiotensin maturation |
| A | 0002019 | biological_process | regulation of renal output by angiotensin |
| A | 0002446 | biological_process | neutrophil mediated immunity |
| A | 0002474 | biological_process | antigen processing and presentation of peptide antigen via MHC class I |
| A | 0003081 | biological_process | regulation of systemic arterial blood pressure by renin-angiotensin |
| A | 0003084 | biological_process | positive regulation of systemic arterial blood pressure |
| A | 0004175 | molecular_function | endopeptidase activity |
| A | 0004180 | molecular_function | carboxypeptidase activity |
| A | 0004222 | molecular_function | metalloendopeptidase activity |
| A | 0005516 | molecular_function | calmodulin binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005615 | cellular_component | extracellular space |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005764 | cellular_component | lysosome |
| A | 0005768 | cellular_component | endosome |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006508 | biological_process | proteolysis |
| A | 0006518 | biological_process | peptide metabolic process |
| A | 0007283 | biological_process | spermatogenesis |
| A | 0007565 | biological_process | female pregnancy |
| A | 0008217 | biological_process | regulation of blood pressure |
| A | 0008233 | molecular_function | peptidase activity |
| A | 0008237 | molecular_function | metallopeptidase activity |
| A | 0008238 | molecular_function | exopeptidase activity |
| A | 0008240 | molecular_function | tripeptidyl-peptidase activity |
| A | 0008241 | molecular_function | peptidyl-dipeptidase activity |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0008584 | biological_process | male gonad development |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0009792 | biological_process | embryo development ending in birth or egg hatching |
| A | 0009897 | cellular_component | external side of plasma membrane |
| A | 0009925 | cellular_component | basal plasma membrane |
| A | 0010608 | biological_process | post-transcriptional regulation of gene expression |
| A | 0010629 | biological_process | negative regulation of gene expression |
| A | 0010814 | biological_process | substance P catabolic process |
| A | 0010815 | biological_process | bradykinin catabolic process |
| A | 0014910 | biological_process | regulation of smooth muscle cell migration |
| A | 0016020 | cellular_component | membrane |
| A | 0019229 | biological_process | regulation of vasoconstriction |
| A | 0030324 | biological_process | lung development |
| A | 0031100 | biological_process | animal organ regeneration |
| A | 0031404 | molecular_function | chloride ion binding |
| A | 0031434 | molecular_function | mitogen-activated protein kinase kinase binding |
| A | 0031526 | cellular_component | brush border membrane |
| A | 0031667 | biological_process | response to nutrient levels |
| A | 0031711 | molecular_function | bradykinin receptor binding |
| A | 0031982 | cellular_component | vesicle |
| A | 0032496 | biological_process | response to lipopolysaccharide |
| A | 0032943 | biological_process | mononuclear cell proliferation |
| A | 0034616 | biological_process | response to laminar fluid shear stress |
| A | 0038166 | biological_process | angiotensin-activated signaling pathway |
| A | 0042310 | biological_process | vasoconstriction |
| A | 0042445 | biological_process | hormone metabolic process |
| A | 0042447 | biological_process | hormone catabolic process |
| A | 0042755 | biological_process | eating behavior |
| A | 0043065 | biological_process | positive regulation of apoptotic process |
| A | 0043171 | biological_process | peptide catabolic process |
| A | 0045777 | biological_process | positive regulation of blood pressure |
| A | 0045907 | biological_process | positive regulation of vasoconstriction |
| A | 0046325 | biological_process | negative regulation of glucose import |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048167 | biological_process | regulation of synaptic plasticity |
| A | 0048286 | biological_process | lung alveolus development |
| A | 0050435 | biological_process | amyloid-beta metabolic process |
| A | 0050482 | biological_process | arachidonic acid secretion |
| A | 0050769 | biological_process | positive regulation of neurogenesis |
| A | 0051019 | molecular_function | mitogen-activated protein kinase binding |
| A | 0060047 | biological_process | heart contraction |
| A | 0060177 | biological_process | regulation of angiotensin metabolic process |
| A | 0060218 | biological_process | hematopoietic stem cell differentiation |
| A | 0060978 | biological_process | angiogenesis involved in coronary vascular morphogenesis |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0070573 | molecular_function | metallodipeptidase activity |
| A | 0071333 | biological_process | cellular response to glucose stimulus |
| A | 0071548 | biological_process | response to dexamethasone |
| A | 0071838 | biological_process | cell proliferation in bone marrow |
| A | 0086091 | biological_process | regulation of heart rate by cardiac conduction |
| A | 0090281 | biological_process | negative regulation of calcium ion import |
| A | 0097066 | biological_process | response to thyroid hormone |
| A | 0097225 | cellular_component | sperm midpiece |
| A | 0097746 | biological_process | blood vessel diameter maintenance |
| A | 1901363 | molecular_function | heterocyclic compound binding |
| A | 1902033 | biological_process | regulation of hematopoietic stem cell proliferation |
| A | 1903597 | biological_process | negative regulation of gap junction assembly |
| A | 1904045 | biological_process | cellular response to aldosterone |
| A | 2000170 | biological_process | positive regulation of peptidyl-cysteine S-nitrosylation |
Functional Information from PROSITE/UniProt
| site_id | PS00142 |
| Number of Residues | 10 |
| Details | ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. TVHHEMGHIQ |
| Chain | Residue | Details |
| A | THR358-GLN367 | |
| A | VAL956-GLN965 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton acceptor 1 => ECO:0000255|PROSITE-ProRule:PRU01355, ECO:0000269|PubMed:12540854, ECO:0000305|PubMed:16154999, ECO:0000305|PubMed:19773553 |
| Chain | Residue | Details |
| A | GLU362 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor 1 => ECO:0000255|PROSITE-ProRule:PRU01355 |
| Chain | Residue | Details |
| A | HIS491 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton acceptor 2 => ECO:0000255|PROSITE-ProRule:PRU01355, ECO:0000269|PubMed:12540854, ECO:0000305|PubMed:16154999, ECO:0000305|PubMed:19773553 |
| Chain | Residue | Details |
| A | GLU960 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 1 |
| Details | ACT_SITE: Proton donor 2 => ECO:0000255|PROSITE-ProRule:PRU01355 |
| Chain | Residue | Details |
| A | HIS1089 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01355, ECO:0000269|PubMed:16476442 |
| Chain | Residue | Details |
| A | TYR202 | |
| A | ARG500 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 3 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01355, ECO:0000269|PubMed:16476442, ECO:0000269|PubMed:20826823, ECO:0000269|PubMed:26403559, ECO:0007744|PDB:2OC2, ECO:0007744|PDB:3NXQ |
| Chain | Residue | Details |
| A | HIS361 | |
| A | HIS365 | |
| A | GLU389 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01355, ECO:0000269|PubMed:12540854 |
| Chain | Residue | Details |
| A | ARG762 | |
| A | TYR800 | |
| A | TRP1061 | |
| A | ARG1065 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01355, ECO:0000269|PubMed:12540854, ECO:0000269|PubMed:16476442, ECO:0000269|PubMed:23056909, ECO:0007744|PDB:4APH |
| Chain | Residue | Details |
| A | HIS959 | |
| A | HIS963 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01355, ECO:0000269|PubMed:12540854, ECO:0000269|PubMed:16476442, ECO:0000269|PubMed:23056909, ECO:0000269|PubMed:7961923, ECO:0007744|PDB:4APH |
| Chain | Residue | Details |
| A | GLU987 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU01355, ECO:0000269|PubMed:12540854, ECO:0000305|PubMed:11432860 |
| Chain | Residue | Details |
| A | ARG1098 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | SITE: Not glycosylated => ECO:0000269|PubMed:20826823 |
| Chain | Residue | Details |
| A | ASN494 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 1 |
| Details | SITE: Cleavage => ECO:0000269|PubMed:7499427, ECO:0000269|PubMed:8253769 |
| Chain | Residue | Details |
| A | ARG1137 |
| site_id | SWS_FT_FI13 |
| Number of Residues | 1 |
| Details | SITE: Not glycosylated => ECO:0000269|PubMed:9013598 |
| Chain | Residue | Details |
| A | ASN1196 |
| site_id | SWS_FT_FI14 |
| Number of Residues | 1 |
| Details | SITE: Cleavage => ECO:0000269|PubMed:10769174 |
| Chain | Residue | Details |
| A | ARG1203 |
| site_id | SWS_FT_FI15 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:20826823, ECO:0000305|PubMed:9013598 |
| Chain | Residue | Details |
| A | ASN9 |
| site_id | SWS_FT_FI16 |
| Number of Residues | 2 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16476442, ECO:0000269|PubMed:20826823, ECO:0000269|PubMed:9013598 |
| Chain | Residue | Details |
| A | ASN25 | |
| A | ASN117 |
| site_id | SWS_FT_FI17 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16476442, ECO:0000269|PubMed:20826823, ECO:0000269|PubMed:26403559, ECO:0007744|PDB:3NXQ |
| Chain | Residue | Details |
| A | ASN45 |
| site_id | SWS_FT_FI18 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:20826823, ECO:0000269|PubMed:9013598 |
| Chain | Residue | Details |
| A | ASN82 |
| site_id | SWS_FT_FI19 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:20826823 |
| Chain | Residue | Details |
| A | ASN131 |
| site_id | SWS_FT_FI20 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16476442 |
| Chain | Residue | Details |
| A | ASN289 |
| site_id | SWS_FT_FI21 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:20826823, ECO:0000269|PubMed:26403559, ECO:0007744|PDB:3NXQ |
| Chain | Residue | Details |
| A | ASN416 |
| site_id | SWS_FT_FI22 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16335952, ECO:0000269|PubMed:16476442, ECO:0000269|PubMed:20826823, ECO:0000269|PubMed:26403559, ECO:0000269|PubMed:9013598, ECO:0007744|PDB:3NXQ |
| Chain | Residue | Details |
| A | ASN480 |
| site_id | SWS_FT_FI23 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:23056909, ECO:0007744|PDB:4APH |
| Chain | Residue | Details |
| A | ASN648 |
| site_id | SWS_FT_FI24 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) (complex) asparagine => ECO:0000269|PubMed:16335952, ECO:0000269|PubMed:9013598 |
| Chain | Residue | Details |
| A | ASN666 |
| site_id | SWS_FT_FI25 |
| Number of Residues | 1 |
| Details | CARBOHYD: N-linked (GlcNAc...) (complex) asparagine => ECO:0000269|PubMed:16335952, ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:23056909, ECO:0000269|PubMed:9013598, ECO:0007744|PDB:4APH |
| Chain | Residue | Details |
| A | ASN685 |
| site_id | SWS_FT_FI26 |
| Number of Residues | 3 |
| Details | CARBOHYD: N-linked (GlcNAc...) asparagine; partial => ECO:0000269|PubMed:9013598 |
| Chain | Residue | Details |
| A | ASN731 | |
| A | ASN913 | |
| A | ASN1162 |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 8 |
| Details | M-CSA 170 |
| Chain | Residue | Details |
| A | HIS929 | electrostatic stabiliser, hydrogen bond acceptor |
| A | ALA930 | electrostatic stabiliser, hydrogen bond acceptor |
| A | HIS959 | metal ligand |
| A | GLU960 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, proton acceptor, proton donor |
| A | HIS963 | metal ligand |
| A | GLU987 | metal ligand |
| A | HIS1089 | electrostatic stabiliser, hydrogen bond donor |
| A | TYR1099 | electrostatic stabiliser, hydrogen bond donor |
