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7P8P

Crystal structure of Fhit covalently bound to a nucleotide

Functional Information from GO Data
ChainGOidnamespacecontents
A0000166molecular_functionnucleotide binding
A0001650cellular_componentfibrillar center
A0003824molecular_functioncatalytic activity
A0005515molecular_functionprotein binding
A0005634cellular_componentnucleus
A0005737cellular_componentcytoplasm
A0005739cellular_componentmitochondrion
A0005829cellular_componentcytosol
A0005886cellular_componentplasma membrane
A0006163biological_processpurine nucleotide metabolic process
A0006915biological_processapoptotic process
A0015964biological_processdiadenosine triphosphate catabolic process
A0016740molecular_functiontransferase activity
A0016787molecular_functionhydrolase activity
A0031625molecular_functionubiquitin protein ligase binding
A0032435biological_processnegative regulation of proteasomal ubiquitin-dependent protein catabolic process
A0042802molecular_functionidentical protein binding
A0043530molecular_functionadenosine 5'-monophosphoramidase activity
A0047352molecular_functionadenylylsulfate-ammonia adenylyltransferase activity
A0047627molecular_functionadenylylsulfatase activity
A0047710molecular_functionbis(5'-adenosyl)-triphosphatase activity
A0072332biological_processintrinsic apoptotic signaling pathway by p53 class mediator
B0000166molecular_functionnucleotide binding
B0001650cellular_componentfibrillar center
B0003824molecular_functioncatalytic activity
B0005515molecular_functionprotein binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005829cellular_componentcytosol
B0005886cellular_componentplasma membrane
B0006163biological_processpurine nucleotide metabolic process
B0006915biological_processapoptotic process
B0015964biological_processdiadenosine triphosphate catabolic process
B0016740molecular_functiontransferase activity
B0016787molecular_functionhydrolase activity
B0031625molecular_functionubiquitin protein ligase binding
B0032435biological_processnegative regulation of proteasomal ubiquitin-dependent protein catabolic process
B0042802molecular_functionidentical protein binding
B0043530molecular_functionadenosine 5'-monophosphoramidase activity
B0047352molecular_functionadenylylsulfate-ammonia adenylyltransferase activity
B0047627molecular_functionadenylylsulfatase activity
B0047710molecular_functionbis(5'-adenosyl)-triphosphatase activity
B0072332biological_processintrinsic apoptotic signaling pathway by p53 class mediator
C0000166molecular_functionnucleotide binding
C0001650cellular_componentfibrillar center
C0003824molecular_functioncatalytic activity
C0005515molecular_functionprotein binding
C0005634cellular_componentnucleus
C0005737cellular_componentcytoplasm
C0005739cellular_componentmitochondrion
C0005829cellular_componentcytosol
C0005886cellular_componentplasma membrane
C0006163biological_processpurine nucleotide metabolic process
C0006915biological_processapoptotic process
C0015964biological_processdiadenosine triphosphate catabolic process
C0016740molecular_functiontransferase activity
C0016787molecular_functionhydrolase activity
C0031625molecular_functionubiquitin protein ligase binding
C0032435biological_processnegative regulation of proteasomal ubiquitin-dependent protein catabolic process
C0042802molecular_functionidentical protein binding
C0043530molecular_functionadenosine 5'-monophosphoramidase activity
C0047352molecular_functionadenylylsulfate-ammonia adenylyltransferase activity
C0047627molecular_functionadenylylsulfatase activity
C0047710molecular_functionbis(5'-adenosyl)-triphosphatase activity
C0072332biological_processintrinsic apoptotic signaling pathway by p53 class mediator
D0000166molecular_functionnucleotide binding
D0001650cellular_componentfibrillar center
D0003824molecular_functioncatalytic activity
D0005515molecular_functionprotein binding
D0005634cellular_componentnucleus
D0005737cellular_componentcytoplasm
D0005739cellular_componentmitochondrion
D0005829cellular_componentcytosol
D0005886cellular_componentplasma membrane
D0006163biological_processpurine nucleotide metabolic process
D0006915biological_processapoptotic process
D0015964biological_processdiadenosine triphosphate catabolic process
D0016740molecular_functiontransferase activity
D0016787molecular_functionhydrolase activity
D0031625molecular_functionubiquitin protein ligase binding
D0032435biological_processnegative regulation of proteasomal ubiquitin-dependent protein catabolic process
D0042802molecular_functionidentical protein binding
D0043530molecular_functionadenosine 5'-monophosphoramidase activity
D0047352molecular_functionadenylylsulfate-ammonia adenylyltransferase activity
D0047627molecular_functionadenylylsulfatase activity
D0047710molecular_functionbis(5'-adenosyl)-triphosphatase activity
D0072332biological_processintrinsic apoptotic signaling pathway by p53 class mediator
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsACT_SITE: Tele-AMP-histidine intermediate => ECO:0000269|PubMed:15182206, ECO:0000269|PubMed:9323207
ChainResidueDetails
AASN96
BASN96
CASN96
DASN96

site_idSWS_FT_FI2
Number of Residues4
DetailsBINDING: BINDING => ECO:0000269|PubMed:9261067, ECO:0007744|PDB:3FIT
ChainResidueDetails
ACYS8
BCYS8
CCYS8
DCYS8

site_idSWS_FT_FI3
Number of Residues16
DetailsBINDING: BINDING => ECO:0000269|PubMed:9323207, ECO:0007744|PDB:5FIT
ChainResidueDetails
BASN27
BGLN83
BGLY89
BHIS98
CASN27
CGLN83
CGLY89
CHIS98
DASN27
DGLN83
DGLY89
DHIS98
AASN27
AGLN83
AGLY89
AHIS98

site_idSWS_FT_FI4
Number of Residues4
DetailsSITE: Important for induction of apoptosis => ECO:0000269|PubMed:16407838
ChainResidueDetails
ATYR114
BTYR114
CTYR114
DTYR114

site_idSWS_FT_FI5
Number of Residues4
DetailsMOD_RES: Phosphotyrosine; by SRC => ECO:0000269|PubMed:15007172
ChainResidueDetails
ATYR114
BTYR114
CTYR114
DTYR114

site_idSWS_FT_FI6
Number of Residues4
DetailsMOD_RES: Phosphotyrosine => ECO:0000269|PubMed:15007172
ChainResidueDetails
ATYR145
BTYR145
CTYR145
DTYR145

Catalytic Information from CSA
site_idMCSA1
Number of Residues4
DetailsM-CSA 101
ChainResidueDetails
AGLN83electrostatic stabiliser, hydrogen bond donor
AHIS94electrostatic stabiliser, increase electrophilicity, increase nucleophilicity
AASN96metal ligand, nucleofuge, nucleophile
AHIS98electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor

site_idMCSA2
Number of Residues4
DetailsM-CSA 101
ChainResidueDetails
BGLN83electrostatic stabiliser, hydrogen bond donor
BHIS94electrostatic stabiliser, increase electrophilicity, increase nucleophilicity
BASN96metal ligand, nucleofuge, nucleophile
BHIS98electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor

site_idMCSA3
Number of Residues4
DetailsM-CSA 101
ChainResidueDetails
CGLN83electrostatic stabiliser, hydrogen bond donor
CHIS94electrostatic stabiliser, increase electrophilicity, increase nucleophilicity
CASN96metal ligand, nucleofuge, nucleophile
CHIS98electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor

site_idMCSA4
Number of Residues4
DetailsM-CSA 101
ChainResidueDetails
DGLN83electrostatic stabiliser, hydrogen bond donor
DHIS94electrostatic stabiliser, increase electrophilicity, increase nucleophilicity
DASN96metal ligand, nucleofuge, nucleophile
DHIS98electrostatic stabiliser, hydrogen bond donor, proton acceptor, proton donor

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PDB entries from 2024-06-12

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