7P47

Structure of the E3 ligase Smc5/Nse2 in complex with Ubc9-SUMO thioester mimetic

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000724	biological_process	double-strand break repair via homologous recombination
A	0008270	molecular_function	zinc ion binding
A	0019789	molecular_function	SUMO transferase activity
A	0030915	cellular_component	Smc5-Smc6 complex
C	0000022	biological_process	mitotic spindle elongation
C	0000794	cellular_component	condensed nuclear chromosome
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0016740	molecular_function	transferase activity
C	0016925	biological_process	protein sumoylation
C	0019789	molecular_function	SUMO transferase activity
C	0051301	biological_process	cell division
C	0061656	molecular_function	SUMO conjugating enzyme activity
D	0000794	cellular_component	condensed nuclear chromosome
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005940	cellular_component	septin ring
D	0016925	biological_process	protein sumoylation
D	0031386	molecular_function	protein tag activity
D	0042802	molecular_function	identical protein binding
D	0044389	molecular_function	ubiquitin-like protein ligase binding
E	0000794	cellular_component	condensed nuclear chromosome
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005940	cellular_component	septin ring
E	0016925	biological_process	protein sumoylation
E	0031386	molecular_function	protein tag activity
E	0042802	molecular_function	identical protein binding
E	0044389	molecular_function	ubiquitin-like protein ligase binding

Functional Information from PROSITE/UniProt

site_id	PS00183
Number of Residues	16
Details	UBC_1 Ubiquitin-conjugating (UBC) active site signature. YHPNVyps.GtICLsiL

Chain	Residue	Details
C	TYR82-LEU97

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17287358, ECO:0007744\|PubMed:17330950

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:17287358

site_id	SWS_FT_FI3
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins)