7NSG

Structure of human excitatory amino acid transporter 3 (EAAT3) in complex with HIP-B

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005313	molecular_function	L-glutamate transmembrane transporter activity
A	0005314	molecular_function	high-affinity L-glutamate transmembrane transporter activity
A	0005515	molecular_function	protein binding
A	0005886	cellular_component	plasma membrane
A	0006836	biological_process	neurotransmitter transport
A	0006865	biological_process	amino acid transport
A	0007268	biological_process	chemical synaptic transmission
A	0010460	biological_process	positive regulation of heart rate
A	0015108	molecular_function	chloride transmembrane transporter activity
A	0015183	molecular_function	L-aspartate transmembrane transporter activity
A	0015293	molecular_function	symporter activity
A	0015501	molecular_function	glutamate:sodium symporter activity
A	0015813	biological_process	L-glutamate transmembrane transport
A	0016020	cellular_component	membrane
A	0016324	cellular_component	apical plasma membrane
A	0030424	cellular_component	axon
A	0030425	cellular_component	dendrite
A	0031901	cellular_component	early endosome membrane
A	0031902	cellular_component	late endosome membrane
A	0032279	cellular_component	asymmetric synapse
A	0033229	molecular_function	L-cysteine transmembrane transporter activity
A	0035633	biological_process	maintenance of blood-brain barrier
A	0042883	biological_process	L-cysteine transport
A	0043025	cellular_component	neuronal cell body
A	0043083	cellular_component	synaptic cleft
A	0043197	cellular_component	dendritic spine
A	0043198	cellular_component	dendritic shaft
A	0043204	cellular_component	perikaryon
A	0043679	cellular_component	axon terminus
A	0045177	cellular_component	apical part of cell
A	0045202	cellular_component	synapse
A	0048514	biological_process	blood vessel morphogenesis
A	0051938	biological_process	L-glutamate import
A	0055037	cellular_component	recycling endosome
A	0055038	cellular_component	recycling endosome membrane
A	0070062	cellular_component	extracellular exosome
A	0070633	biological_process	transepithelial transport
A	0070777	biological_process	D-aspartate transmembrane transport
A	0070778	biological_process	L-aspartate transmembrane transport
A	0070779	biological_process	D-aspartate import across plasma membrane
A	0071407	biological_process	obsolete cellular response to organic cyclic compound
A	0071944	cellular_component	cell periphery
A	0097386	cellular_component	glial cell projection
A	0097440	cellular_component	apical dendrite
A	0098657	biological_process	import into cell
A	0098712	biological_process	L-glutamate import across plasma membrane
A	0098793	cellular_component	presynapse
A	0099544	cellular_component	perisynaptic space
A	0140009	biological_process	L-aspartate import across plasma membrane
A	0140010	molecular_function	D-aspartate transmembrane transporter activity
A	0150002	cellular_component	distal dendrite
A	0150104	biological_process	transport across blood-brain barrier
A	1902476	biological_process	chloride transmembrane transport
A	1903712	biological_process	L-cysteine transmembrane transport
A	1990635	cellular_component	proximal dendrite
B	0005313	molecular_function	L-glutamate transmembrane transporter activity
B	0005314	molecular_function	high-affinity L-glutamate transmembrane transporter activity
B	0005515	molecular_function	protein binding
B	0005886	cellular_component	plasma membrane
B	0006836	biological_process	neurotransmitter transport
B	0006865	biological_process	amino acid transport
B	0007268	biological_process	chemical synaptic transmission
B	0010460	biological_process	positive regulation of heart rate
B	0015108	molecular_function	chloride transmembrane transporter activity
B	0015183	molecular_function	L-aspartate transmembrane transporter activity
B	0015293	molecular_function	symporter activity
B	0015501	molecular_function	glutamate:sodium symporter activity
B	0015813	biological_process	L-glutamate transmembrane transport
B	0016020	cellular_component	membrane
B	0016324	cellular_component	apical plasma membrane
B	0030424	cellular_component	axon
B	0030425	cellular_component	dendrite
B	0031901	cellular_component	early endosome membrane
B	0031902	cellular_component	late endosome membrane
B	0032279	cellular_component	asymmetric synapse
B	0033229	molecular_function	L-cysteine transmembrane transporter activity
B	0035633	biological_process	maintenance of blood-brain barrier
B	0042883	biological_process	L-cysteine transport
B	0043025	cellular_component	neuronal cell body
B	0043083	cellular_component	synaptic cleft
B	0043197	cellular_component	dendritic spine
B	0043198	cellular_component	dendritic shaft
B	0043204	cellular_component	perikaryon
B	0043679	cellular_component	axon terminus
B	0045177	cellular_component	apical part of cell
B	0045202	cellular_component	synapse
B	0048514	biological_process	blood vessel morphogenesis
B	0051938	biological_process	L-glutamate import
B	0055037	cellular_component	recycling endosome
B	0055038	cellular_component	recycling endosome membrane
B	0070062	cellular_component	extracellular exosome
B	0070633	biological_process	transepithelial transport
B	0070777	biological_process	D-aspartate transmembrane transport
B	0070778	biological_process	L-aspartate transmembrane transport
B	0070779	biological_process	D-aspartate import across plasma membrane
B	0071407	biological_process	obsolete cellular response to organic cyclic compound
B	0071944	cellular_component	cell periphery
B	0097386	cellular_component	glial cell projection
B	0097440	cellular_component	apical dendrite
B	0098657	biological_process	import into cell
B	0098712	biological_process	L-glutamate import across plasma membrane
B	0098793	cellular_component	presynapse
B	0099544	cellular_component	perisynaptic space
B	0140009	biological_process	L-aspartate import across plasma membrane
B	0140010	molecular_function	D-aspartate transmembrane transporter activity
B	0150002	cellular_component	distal dendrite
B	0150104	biological_process	transport across blood-brain barrier
B	1902476	biological_process	chloride transmembrane transport
B	1903712	biological_process	L-cysteine transmembrane transport
B	1990635	cellular_component	proximal dendrite
C	0005313	molecular_function	L-glutamate transmembrane transporter activity
C	0005314	molecular_function	high-affinity L-glutamate transmembrane transporter activity
C	0005515	molecular_function	protein binding
C	0005886	cellular_component	plasma membrane
C	0006836	biological_process	neurotransmitter transport
C	0006865	biological_process	amino acid transport
C	0007268	biological_process	chemical synaptic transmission
C	0010460	biological_process	positive regulation of heart rate
C	0015108	molecular_function	chloride transmembrane transporter activity
C	0015183	molecular_function	L-aspartate transmembrane transporter activity
C	0015293	molecular_function	symporter activity
C	0015501	molecular_function	glutamate:sodium symporter activity
C	0015813	biological_process	L-glutamate transmembrane transport
C	0016020	cellular_component	membrane
C	0016324	cellular_component	apical plasma membrane
C	0030424	cellular_component	axon
C	0030425	cellular_component	dendrite
C	0031901	cellular_component	early endosome membrane
C	0031902	cellular_component	late endosome membrane
C	0032279	cellular_component	asymmetric synapse
C	0033229	molecular_function	L-cysteine transmembrane transporter activity
C	0035633	biological_process	maintenance of blood-brain barrier
C	0042883	biological_process	L-cysteine transport
C	0043025	cellular_component	neuronal cell body
C	0043083	cellular_component	synaptic cleft
C	0043197	cellular_component	dendritic spine
C	0043198	cellular_component	dendritic shaft
C	0043204	cellular_component	perikaryon
C	0043679	cellular_component	axon terminus
C	0045177	cellular_component	apical part of cell
C	0045202	cellular_component	synapse
C	0048514	biological_process	blood vessel morphogenesis
C	0051938	biological_process	L-glutamate import
C	0055037	cellular_component	recycling endosome
C	0055038	cellular_component	recycling endosome membrane
C	0070062	cellular_component	extracellular exosome
C	0070633	biological_process	transepithelial transport
C	0070777	biological_process	D-aspartate transmembrane transport
C	0070778	biological_process	L-aspartate transmembrane transport
C	0070779	biological_process	D-aspartate import across plasma membrane
C	0071407	biological_process	obsolete cellular response to organic cyclic compound
C	0071944	cellular_component	cell periphery
C	0097386	cellular_component	glial cell projection
C	0097440	cellular_component	apical dendrite
C	0098657	biological_process	import into cell
C	0098712	biological_process	L-glutamate import across plasma membrane
C	0098793	cellular_component	presynapse
C	0099544	cellular_component	perisynaptic space
C	0140009	biological_process	L-aspartate import across plasma membrane
C	0140010	molecular_function	D-aspartate transmembrane transporter activity
C	0150002	cellular_component	distal dendrite
C	0150104	biological_process	transport across blood-brain barrier
C	1902476	biological_process	chloride transmembrane transport
C	1903712	biological_process	L-cysteine transmembrane transport
C	1990635	cellular_component	proximal dendrite

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue UR5 A 501

Chain	Residue
A	SER333
A	ASP444
A	ARG447
A	THR448
A	ASN451
A	UR8502

---

site_id	AC2
Number of Residues	7
Details	binding site for residue UR8 A 502

Chain	Residue
A	ARG447
A	THR448
A	ASN451
A	UR5501
A	THR370
A	PRO412
A	ASP444

---

site_id	AC3
Number of Residues	2
Details	binding site for residue Y01 A 503

Chain	Residue
A	VAL96
A	LEU465

---

site_id	AC4
Number of Residues	4
Details	binding site for residue PC1 A 504

Chain	Residue
A	VAL86
A	PHE99
A	LEU224
A	PC1505

---

site_id	AC5
Number of Residues	1
Details	binding site for residue PC1 A 505

Chain	Residue
A	PC1504

---

site_id	AC6
Number of Residues	2
Details	binding site for residue PC1 A 507

Chain	Residue
A	VAL114
A	SER398

---

site_id	AC7
Number of Residues	2
Details	binding site for residue PC1 A 508

Chain	Residue
A	TRP19
A	LEU65

---

site_id	AC8
Number of Residues	6
Details	binding site for residue UR5 B 501

Chain	Residue
B	SER333
B	ASP444
B	ARG447
B	THR448
B	ASN451
B	UR8502

---

site_id	AC9
Number of Residues	7
Details	binding site for residue UR8 B 502

Chain	Residue
B	THR370
B	PRO412
B	ASP444
B	ARG447
B	THR448
B	ASN451
B	UR5501

---

site_id	AD1
Number of Residues	2
Details	binding site for residue Y01 B 503

Chain	Residue
B	VAL96
B	LEU465

---

site_id	AD2
Number of Residues	4
Details	binding site for residue PC1 B 504

Chain	Residue
B	VAL86
B	PHE99
B	LEU224
B	PC1505

---

site_id	AD3
Number of Residues	1
Details	binding site for residue PC1 B 505

Chain	Residue
B	PC1504

---

site_id	AD4
Number of Residues	2
Details	binding site for residue PC1 B 507

Chain	Residue
B	VAL114
B	SER398

---

site_id	AD5
Number of Residues	2
Details	binding site for residue PC1 B 508

Chain	Residue
B	TRP19
B	LEU65

---

site_id	AD6
Number of Residues	6
Details	binding site for residue UR5 C 501

Chain	Residue
C	SER333
C	ASP444
C	ARG447
C	THR448
C	ASN451
C	UR8502

---

site_id	AD7
Number of Residues	8
Details	binding site for residue UR8 C 502

Chain	Residue
C	SER333
C	THR370
C	PRO412
C	ASP444
C	ARG447
C	THR448
C	ASN451
C	UR5501

---

site_id	AD8
Number of Residues	2
Details	binding site for residue Y01 C 503

Chain	Residue
C	VAL96
C	LEU465

---

site_id	AD9
Number of Residues	4
Details	binding site for residue PC1 C 504

Chain	Residue
C	VAL86
C	PHE99
C	LEU224
C	PC1505

---

site_id	AE1
Number of Residues	1
Details	binding site for residue PC1 C 505

Chain	Residue
C	PC1504

---

site_id	AE2
Number of Residues	2
Details	binding site for residue PC1 C 507

Chain	Residue
C	VAL114
C	SER398

---

site_id	AE3
Number of Residues	3
Details	binding site for residue PC1 C 508

Chain	Residue
B	PHE218
C	TRP19
C	LEU65

---

Functional Information from PROSITE/UniProt

site_id	PS00713
Number of Residues	15
Details	NA_DICARBOXYL_SYMP_1 Sodium:dicarboxylate symporter family signature 1. P.GEiLMrMLKLIIlP

Chain	Residue	Details
A	PRO55-PRO69

---

site_id	PS00714
Number of Residues	24
Details	NA_DICARBOXYL_SYMP_2 Sodium:dicarboxylate symporter family signature 2. PvGaTiNMDGTaLYeaVaaVFIAQ

Chain	Residue	Details
A	PRO360-GLN383

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	177
Details	Transmembrane: {"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	195
Details	Topological domain: {"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	90
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	69
Details	Transmembrane: {"description":"Helical; Name=4","evidences":[{"source":"UniProtKB","id":"P43003","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	81
Details	Transmembrane: {"description":"Helical; Name=5","evidences":[{"source":"UniProtKB","id":"P43003","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	63
Details	Transmembrane: {"description":"Helical; Name=6","evidences":[{"source":"UniProtKB","id":"P43003","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	189
Details	Intramembrane: {"description":"Discontinuously helical","evidences":[{"source":"UniProtKB","id":"P43003","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	78
Details	Transmembrane: {"description":"Helical; Name=7","evidences":[{"source":"UniProtKB","id":"P43003","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	63
Details	Transmembrane: {"description":"Helical; Name=8","evidences":[{"source":"UniProtKB","id":"P43003","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	39
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33658209","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6X2Z","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	12
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33658209","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6X2Z","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"6X3E","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33658209","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6X3E","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	3
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---