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7MFD

Autoinhibited BRAF:(14-3-3)2:MEK complex with the BRAF RBD resolved

Functional Information from GO Data
ChainGOidnamespacecontents
A0000165biological_processMAPK cascade
A0001934biological_processpositive regulation of protein phosphorylation
A0002318biological_processmyeloid progenitor cell differentiation
A0004672molecular_functionprotein kinase activity
A0004674molecular_functionprotein serine/threonine kinase activity
A0004708molecular_functionMAP kinase kinase activity
A0004709molecular_functionMAP kinase kinase kinase activity
A0005509molecular_functioncalcium ion binding
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0005634cellular_componentnucleus
A0005737cellular_componentcytoplasm
A0005739cellular_componentmitochondrion
A0005829cellular_componentcytosol
A0005886cellular_componentplasma membrane
A0006338biological_processchromatin remodeling
A0006468biological_processprotein phosphorylation
A0007165biological_processsignal transduction
A0007173biological_processepidermal growth factor receptor signaling pathway
A0008542biological_processvisual learning
A0009887biological_processanimal organ morphogenesis
A0010628biological_processpositive regulation of gene expression
A0010764biological_processnegative regulation of fibroblast migration
A0010828biological_processpositive regulation of glucose transmembrane transport
A0016079biological_processsynaptic vesicle exocytosis
A0016310biological_processphosphorylation
A0030154biological_processcell differentiation
A0030878biological_processthyroid gland development
A0031267molecular_functionsmall GTPase binding
A0031434molecular_functionmitogen-activated protein kinase kinase binding
A0033077biological_processT cell differentiation in thymus
A0033138biological_processpositive regulation of peptidyl-serine phosphorylation
A0034446biological_processsubstrate adhesion-dependent cell spreading
A0035019biological_processsomatic stem cell population maintenance
A0036211biological_processprotein modification process
A0042127biological_processregulation of cell population proliferation
A0042802molecular_functionidentical protein binding
A0043005cellular_componentneuron projection
A0043066biological_processnegative regulation of apoptotic process
A0043149biological_processstress fiber assembly
A0043231cellular_componentintracellular membrane-bounded organelle
A0043367biological_processCD4-positive, alpha-beta T cell differentiation
A0043368biological_processpositive T cell selection
A0043369biological_processCD4-positive or CD8-positive, alpha-beta T cell lineage commitment
A0043434biological_processresponse to peptide hormone
A0043524biological_processnegative regulation of neuron apoptotic process
A0044024molecular_functionhistone H2AS1 kinase activity
A0044297cellular_componentcell body
A0044877molecular_functionprotein-containing complex binding
A0045580biological_processregulation of T cell differentiation
A0046632biological_processalpha-beta T cell differentiation
A0046872molecular_functionmetal ion binding
A0048538biological_processthymus development
A0048679biological_processregulation of axon regeneration
A0048680biological_processpositive regulation of axon regeneration
A0050772biological_processpositive regulation of axonogenesis
A0050852biological_processT cell receptor signaling pathway
A0051496biological_processpositive regulation of stress fiber assembly
A0051591biological_processresponse to cAMP
A0060291biological_processlong-term synaptic potentiation
A0060323biological_processhead morphogenesis
A0060324biological_processface development
A0070371biological_processERK1 and ERK2 cascade
A0070374biological_processpositive regulation of ERK1 and ERK2 cascade
A0071277biological_processcellular response to calcium ion
A0071466biological_processcellular response to xenobiotic stimulus
A0072577biological_processendothelial cell apoptotic process
A0090150biological_processestablishment of protein localization to membrane
A0097110molecular_functionscaffold protein binding
A0098793cellular_componentpresynapse
A0098794cellular_componentpostsynapse
A0098978cellular_componentglutamatergic synapse
A0099170biological_processpostsynaptic modulation of chemical synaptic transmission
A0106310molecular_functionprotein serine kinase activity
A1900026biological_processpositive regulation of substrate adhesion-dependent cell spreading
A1902531biological_processregulation of intracellular signal transduction
A1990090biological_processcellular response to nerve growth factor stimulus
A2000301biological_processnegative regulation of synaptic vesicle exocytosis
A2000352biological_processnegative regulation of endothelial cell apoptotic process
B0000165biological_processMAPK cascade
B0000187biological_processobsolete activation of MAPK activity
B0004672molecular_functionprotein kinase activity
B0004674molecular_functionprotein serine/threonine kinase activity
B0004708molecular_functionMAP kinase kinase activity
B0004712molecular_functionprotein serine/threonine/tyrosine kinase activity
B0004713molecular_functionprotein tyrosine kinase activity
B0005078molecular_functionMAP-kinase scaffold activity
B0005515molecular_functionprotein binding
B0005524molecular_functionATP binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005769cellular_componentearly endosome
B0005770cellular_componentlate endosome
B0005783cellular_componentendoplasmic reticulum
B0005794cellular_componentGolgi apparatus
B0005813cellular_componentcentrosome
B0005816cellular_componentspindle pole body
B0005829cellular_componentcytosol
B0005856cellular_componentcytoskeleton
B0005886cellular_componentplasma membrane
B0005925cellular_componentfocal adhesion
B0006468biological_processprotein phosphorylation
B0006935biological_processchemotaxis
B0007165biological_processsignal transduction
B0007507biological_processheart development
B0008285biological_processnegative regulation of cell population proliferation
B0010628biological_processpositive regulation of gene expression
B0014044biological_processSchwann cell development
B0016020cellular_componentmembrane
B0016310biological_processphosphorylation
B0021697biological_processcerebellar cortex formation
B0030182biological_processneuron differentiation
B0030216biological_processkeratinocyte differentiation
B0030295molecular_functionprotein kinase activator activity
B0030878biological_processthyroid gland development
B0032872biological_processregulation of stress-activated MAPK cascade
B0035987biological_processendodermal cell differentiation
B0038133biological_processERBB2-ERBB3 signaling pathway
B0042552biological_processmyelination
B0043410biological_processpositive regulation of MAPK cascade
B0043539molecular_functionprotein serine/threonine kinase activator activity
B0044342biological_processtype B pancreatic cell proliferation
B0045893biological_processpositive regulation of DNA-templated transcription
B0048009biological_processinsulin-like growth factor receptor signaling pathway
B0048538biological_processthymus development
B0048679biological_processregulation of axon regeneration
B0048870biological_processcell motility
B0050772biological_processpositive regulation of axonogenesis
B0060020biological_processBergmann glial cell differentiation
B0060324biological_processface development
B0060425biological_processlung morphogenesis
B0060440biological_processtrachea formation
B0060502biological_processepithelial cell proliferation involved in lung morphogenesis
B0060674biological_processplacenta blood vessel development
B0060711biological_processlabyrinthine layer development
B0070371biological_processERK1 and ERK2 cascade
B0070374biological_processpositive regulation of ERK1 and ERK2 cascade
B0071902biological_processpositive regulation of protein serine/threonine kinase activity
B0090170biological_processregulation of Golgi inheritance
B0090398biological_processcellular senescence
B0097110molecular_functionscaffold protein binding
B0106310molecular_functionprotein serine kinase activity
B1903226biological_processpositive regulation of endodermal cell differentiation
B2000641biological_processregulation of early endosome to late endosome transport
C0000122biological_processnegative regulation of transcription by RNA polymerase II
C0001525biological_processangiogenesis
C0003016biological_processrespiratory system process
C0003723molecular_functionRNA binding
C0005515molecular_functionprotein binding
C0005615cellular_componentextracellular space
C0005634cellular_componentnucleus
C0005654cellular_componentnucleoplasm
C0005737cellular_componentcytoplasm
C0005829cellular_componentcytosol
C0005925cellular_componentfocal adhesion
C0006468biological_processprotein phosphorylation
C0006605biological_processprotein targeting
C0007165biological_processsignal transduction
C0008039biological_processsynaptic target recognition
C0008104biological_processprotein localization
C0019901molecular_functionprotein kinase binding
C0019904molecular_functionprotein domain specific binding
C0030324biological_processlung development
C0031625molecular_functionubiquitin protein ligase binding
C0031647biological_processregulation of protein stability
C0031982cellular_componentvesicle
C0035148biological_processtube formation
C0042149biological_processcellular response to glucose starvation
C0042470cellular_componentmelanosome
C0042802molecular_functionidentical protein binding
C0043066biological_processnegative regulation of apoptotic process
C0043067biological_processregulation of programmed cell death
C0044325molecular_functiontransmembrane transporter binding
C0045296molecular_functioncadherin binding
C0045824biological_processnegative regulation of innate immune response
C0050815molecular_functionphosphoserine residue binding
C0051683biological_processestablishment of Golgi localization
C0070062cellular_componentextracellular exosome
C0070371biological_processERK1 and ERK2 cascade
C0070372biological_processregulation of ERK1 and ERK2 cascade
C0072562cellular_componentblood microparticle
C0090128biological_processregulation of synapse maturation
C0090168biological_processGolgi reassembly
C0098686cellular_componenthippocampal mossy fiber to CA3 synapse
C0098978cellular_componentglutamatergic synapse
C0140297molecular_functionDNA-binding transcription factor binding
C0140311molecular_functionprotein sequestering activity
C1900181biological_processnegative regulation of protein localization to nucleus
C1904262biological_processnegative regulation of TORC1 signaling
D0000122biological_processnegative regulation of transcription by RNA polymerase II
D0001525biological_processangiogenesis
D0003016biological_processrespiratory system process
D0003723molecular_functionRNA binding
D0005515molecular_functionprotein binding
D0005615cellular_componentextracellular space
D0005634cellular_componentnucleus
D0005654cellular_componentnucleoplasm
D0005737cellular_componentcytoplasm
D0005829cellular_componentcytosol
D0005925cellular_componentfocal adhesion
D0006468biological_processprotein phosphorylation
D0006605biological_processprotein targeting
D0007165biological_processsignal transduction
D0008039biological_processsynaptic target recognition
D0008104biological_processprotein localization
D0019901molecular_functionprotein kinase binding
D0019904molecular_functionprotein domain specific binding
D0030324biological_processlung development
D0031625molecular_functionubiquitin protein ligase binding
D0031647biological_processregulation of protein stability
D0031982cellular_componentvesicle
D0035148biological_processtube formation
D0042149biological_processcellular response to glucose starvation
D0042470cellular_componentmelanosome
D0042802molecular_functionidentical protein binding
D0043066biological_processnegative regulation of apoptotic process
D0043067biological_processregulation of programmed cell death
D0044325molecular_functiontransmembrane transporter binding
D0045296molecular_functioncadherin binding
D0045824biological_processnegative regulation of innate immune response
D0050815molecular_functionphosphoserine residue binding
D0051683biological_processestablishment of Golgi localization
D0070062cellular_componentextracellular exosome
D0070371biological_processERK1 and ERK2 cascade
D0070372biological_processregulation of ERK1 and ERK2 cascade
D0072562cellular_componentblood microparticle
D0090128biological_processregulation of synapse maturation
D0090168biological_processGolgi reassembly
D0098686cellular_componenthippocampal mossy fiber to CA3 synapse
D0098978cellular_componentglutamatergic synapse
D0140297molecular_functionDNA-binding transcription factor binding
D0140311molecular_functionprotein sequestering activity
D1900181biological_processnegative regulation of protein localization to nucleus
D1904262biological_processnegative regulation of TORC1 signaling
Functional Information from PROSITE/UniProt
site_idPS00107
Number of Residues21
DetailsPROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGSGSFGTVYkGkwhgd.............VAVK
ChainResidueDetails
AILE463-LYS483
BLEU74-LYS97

site_idPS00108
Number of Residues13
DetailsPROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. IiHrDLKsnNIFL
ChainResidueDetails
AILE572-LEU584
BILE186-VAL198

site_idPS00479
Number of Residues46
DetailsZF_DAG_PE_1 Zinc finger phorbol-ester/DAG-type signature. HnFvrktfftlaf.CdfCrklLfqgfr.....CqtCgykfHqrCstevplm..C
ChainResidueDetails
AHIS235-CYS280

site_idPS00796
Number of Residues11
Details1433_1 14-3-3 proteins signature 1. RNLLSVAYKNV
ChainResidueDetails
CARG41-VAL51

site_idPS00797
Number of Residues20
Details1433_2 14-3-3 proteins signature 2. YKDSTLIMQLLRDNLTLWTS
ChainResidueDetails
CTYR211-SER230

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsSITE: Interaction with phosphoserine on interacting protein => ECO:0000250|UniProtKB:P63103
ChainResidueDetails
CARG56
CARG127
DARG56
DARG127

site_idSWS_FT_FI2
Number of Residues2
DetailsMOD_RES: N-acetylmethionine => ECO:0000269|Ref.8, ECO:0007744|PubMed:22223895, ECO:0007744|PubMed:22814378, ECO:0007744|PubMed:25944712
ChainResidueDetails
CMET1
DMET1
BSER150
BLYS192
BASP208

site_idSWS_FT_FI3
Number of Residues4
DetailsMOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861
ChainResidueDetails
CLYS3
CLYS68
DLYS3
DLYS68
ACYS264
AHIS269
ACYS272
ACYS280

site_idSWS_FT_FI4
Number of Residues2
DetailsMOD_RES: Phosphoserine; by PKA and PKB/AKT1 => ECO:0000269|PubMed:11956222, ECO:0000269|PubMed:12865427, ECO:0000269|PubMed:15883165, ECO:0000269|PubMed:16376338
ChainResidueDetails
CSER58
DSER58

site_idSWS_FT_FI5
Number of Residues2
DetailsMOD_RES: Phosphoserine; by MAPK8 => ECO:0000269|PubMed:15071501, ECO:0000269|PubMed:15696159
ChainResidueDetails
CSER184
DSER184

site_idSWS_FT_FI6
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231
ChainResidueDetails
CSER207
DSER207

site_idSWS_FT_FI7
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P63102
ChainResidueDetails
CSER210
DSER210

site_idSWS_FT_FI8
Number of Residues2
DetailsMOD_RES: Phosphothreonine; by CK1 => ECO:0000269|PubMed:31024343, ECO:0000269|PubMed:9360956, ECO:0007744|PubMed:20068231
ChainResidueDetails
CTHR232
DTHR232

site_idSWS_FT_FI9
Number of Residues1
DetailsMOD_RES: Phosphothreonine; by MAPK1 => ECO:0000250|UniProtKB:Q01986
ChainResidueDetails
BTHR292

site_idSWS_FT_FI10
Number of Residues1
DetailsMOD_RES: Phosphoserine; by PAK => ECO:0000269|PubMed:16129686
ChainResidueDetails
BSER298

site_idSWS_FT_FI11
Number of Residues1
DetailsMOD_RES: Phosphothreonine => ECO:0000269|Ref.8
ChainResidueDetails
ATHR396

site_idSWS_FT_FI12
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0000269|Ref.8
ChainResidueDetails
ASER399

site_idSWS_FT_FI13
Number of Residues1
DetailsMOD_RES: Phosphothreonine => ECO:0000269|Ref.8, ECO:0007744|PubMed:16964243, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163
ChainResidueDetails
ATHR401

site_idSWS_FT_FI14
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163
ChainResidueDetails
ASER446

site_idSWS_FT_FI15
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648
ChainResidueDetails
ASER447

site_idSWS_FT_FI16
Number of Residues1
DetailsMOD_RES: Omega-N-methylarginine; by PRMT5 => ECO:0000269|PubMed:21917714
ChainResidueDetails
AARG671

site_idSWS_FT_FI17
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0000269|Ref.8, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163
ChainResidueDetails
ASEP729

site_idSWS_FT_FI18
Number of Residues1
DetailsMOD_RES: Phosphothreonine; by MAPK1 => ECO:0000269|PubMed:19710016
ChainResidueDetails
ATHR753

site_idSWS_FT_FI19
Number of Residues2
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:23907581
ChainResidueDetails
ALYS578

224201

PDB entries from 2024-08-28

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