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7FGW

Toxoplasma gondii dihydrofolate reductase thymidylate synthase (TgDHFR-TS) complexed with pyrimethamine, NADPH and dUMP

Functional Information from GO Data
ChainGOidnamespacecontents
A0004146molecular_functiondihydrofolate reductase activity
A0004799molecular_functionthymidylate synthase activity
A0005739cellular_componentmitochondrion
A0005829cellular_componentcytosol
A0006231biological_processdTMP biosynthetic process
A0006730biological_processone-carbon metabolic process
A0008168molecular_functionmethyltransferase activity
A0009165biological_processnucleotide biosynthetic process
A0016491molecular_functionoxidoreductase activity
A0016741molecular_functiontransferase activity, transferring one-carbon groups
A0032259biological_processmethylation
A0046654biological_processtetrahydrofolate biosynthetic process
B0004146molecular_functiondihydrofolate reductase activity
B0004799molecular_functionthymidylate synthase activity
B0005739cellular_componentmitochondrion
B0005829cellular_componentcytosol
B0006231biological_processdTMP biosynthetic process
B0006730biological_processone-carbon metabolic process
B0008168molecular_functionmethyltransferase activity
B0009165biological_processnucleotide biosynthetic process
B0016491molecular_functionoxidoreductase activity
B0016741molecular_functiontransferase activity, transferring one-carbon groups
B0032259biological_processmethylation
B0046654biological_processtetrahydrofolate biosynthetic process
C0004146molecular_functiondihydrofolate reductase activity
C0004799molecular_functionthymidylate synthase activity
C0005739cellular_componentmitochondrion
C0005829cellular_componentcytosol
C0006231biological_processdTMP biosynthetic process
C0006730biological_processone-carbon metabolic process
C0008168molecular_functionmethyltransferase activity
C0009165biological_processnucleotide biosynthetic process
C0016491molecular_functionoxidoreductase activity
C0016741molecular_functiontransferase activity, transferring one-carbon groups
C0032259biological_processmethylation
C0046654biological_processtetrahydrofolate biosynthetic process
D0004146molecular_functiondihydrofolate reductase activity
D0004799molecular_functionthymidylate synthase activity
D0005739cellular_componentmitochondrion
D0005829cellular_componentcytosol
D0006231biological_processdTMP biosynthetic process
D0006730biological_processone-carbon metabolic process
D0008168molecular_functionmethyltransferase activity
D0009165biological_processnucleotide biosynthetic process
D0016491molecular_functionoxidoreductase activity
D0016741molecular_functiontransferase activity, transferring one-carbon groups
D0032259biological_processmethylation
D0046654biological_processtetrahydrofolate biosynthetic process
Functional Information from PROSITE/UniProt
site_idPS00075
Number of Residues24
DetailsDHFR_1 Dihydrofolate reductase (DHFR) domain signature. GIGinngLPWphlttDfkhFsrvT
ChainResidueDetails
AGLY16-THR39

site_idPS00091
Number of Residues29
DetailsTHYMIDYLATE_SYNTHASE Thymidylate synthase active site. RrmLmtaWNpaaldema.....LpPCHllcQFyV
ChainResidueDetails
AARG469-VAL497

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsACT_SITE: ACT_SITE => ECO:0000250
ChainResidueDetails
ACYS489
BCYS489
CCYS489
DCYS489

site_idSWS_FT_FI2
Number of Residues56
DetailsBINDING: BINDING => ECO:0000250
ChainResidueDetails
AVAL8
AARG344
AHIS490
AGLN509
AASN521
AHIS551
BVAL8
BALA10
BGLY16
BASP31
BARG81
AALA10
BVAL102
BGLY152
BTYR157
BTHR172
BARG344
BHIS490
BGLN509
BASN521
BHIS551
CVAL8
AGLY16
CALA10
CGLY16
CASP31
CARG81
CVAL102
CGLY152
CTYR157
CTHR172
CARG344
CHIS490
AASP31
CGLN509
CASN521
CHIS551
DVAL8
DALA10
DGLY16
DASP31
DARG81
DVAL102
DGLY152
AARG81
DTYR157
DTHR172
DARG344
DHIS490
DGLN509
DASN521
DHIS551
AVAL102
AGLY152
ATYR157
ATHR172

220113

PDB entries from 2024-05-22

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