7DSX

Structure of a human NHE1-CHP1 complex under pH 7.5, bound by cariporide

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0006812	biological_process	monoatomic cation transport
A	0006814	biological_process	sodium ion transport
A	0006885	biological_process	regulation of pH
A	0015297	molecular_function	antiporter activity
A	0015385	molecular_function	sodium:proton antiporter activity
A	0016020	cellular_component	membrane
A	0055085	biological_process	transmembrane transport
A	1902600	biological_process	proton transmembrane transport
B	0006812	biological_process	monoatomic cation transport
B	0006814	biological_process	sodium ion transport
B	0006885	biological_process	regulation of pH
B	0015297	molecular_function	antiporter activity
B	0015385	molecular_function	sodium:proton antiporter activity
B	0016020	cellular_component	membrane
B	0055085	biological_process	transmembrane transport
B	1902600	biological_process	proton transmembrane transport
C	0000139	cellular_component	Golgi membrane
C	0001578	biological_process	microtubule bundle formation
C	0001933	biological_process	negative regulation of protein phosphorylation
C	0004860	molecular_function	protein kinase inhibitor activity
C	0005509	molecular_function	calcium ion binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005783	cellular_component	endoplasmic reticulum
C	0005793	cellular_component	endoplasmic reticulum-Golgi intermediate compartment
C	0005856	cellular_component	cytoskeleton
C	0005886	cellular_component	plasma membrane
C	0005925	cellular_component	focal adhesion
C	0006469	biological_process	negative regulation of protein kinase activity
C	0006611	biological_process	protein export from nucleus
C	0006813	biological_process	potassium ion transport
C	0007264	biological_process	small GTPase-mediated signal transduction
C	0008017	molecular_function	microtubule binding
C	0010923	biological_process	negative regulation of phosphatase activity
C	0012505	cellular_component	endomembrane system
C	0015031	biological_process	protein transport
C	0015385	molecular_function	sodium:proton antiporter activity
C	0015459	molecular_function	potassium channel regulator activity
C	0015630	cellular_component	microtubule cytoskeleton
C	0019900	molecular_function	kinase binding
C	0022406	biological_process	membrane docking
C	0030133	cellular_component	transport vesicle
C	0031122	biological_process	cytoplasmic microtubule organization
C	0031397	biological_process	negative regulation of protein ubiquitination
C	0031953	biological_process	negative regulation of protein autophosphorylation
C	0032088	biological_process	negative regulation of NF-kappaB transcription factor activity
C	0032417	biological_process	positive regulation of sodium:proton antiporter activity
C	0035725	biological_process	sodium ion transmembrane transport
C	0042308	biological_process	negative regulation of protein import into nucleus
C	0045121	cellular_component	membrane raft
C	0046872	molecular_function	metal ion binding
C	0048306	molecular_function	calcium-dependent protein binding
C	0050821	biological_process	protein stabilization
C	0051222	biological_process	positive regulation of protein transport
C	0051453	biological_process	regulation of intracellular pH
C	0060050	biological_process	positive regulation of protein glycosylation
C	0061024	biological_process	membrane organization
C	0061025	biological_process	membrane fusion
C	0070062	cellular_component	extracellular exosome
C	0070885	biological_process	negative regulation of calcineurin-NFAT signaling cascade
C	0071073	biological_process	positive regulation of phospholipid biosynthetic process
C	0071468	biological_process	cellular response to acidic pH
C	0090314	biological_process	positive regulation of protein targeting to membrane
C	1990351	cellular_component	transporter complex
D	0000139	cellular_component	Golgi membrane
D	0001578	biological_process	microtubule bundle formation
D	0001933	biological_process	negative regulation of protein phosphorylation
D	0004860	molecular_function	protein kinase inhibitor activity
D	0005509	molecular_function	calcium ion binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005783	cellular_component	endoplasmic reticulum
D	0005793	cellular_component	endoplasmic reticulum-Golgi intermediate compartment
D	0005856	cellular_component	cytoskeleton
D	0005886	cellular_component	plasma membrane
D	0005925	cellular_component	focal adhesion
D	0006469	biological_process	negative regulation of protein kinase activity
D	0006611	biological_process	protein export from nucleus
D	0006813	biological_process	potassium ion transport
D	0007264	biological_process	small GTPase-mediated signal transduction
D	0008017	molecular_function	microtubule binding
D	0010923	biological_process	negative regulation of phosphatase activity
D	0012505	cellular_component	endomembrane system
D	0015031	biological_process	protein transport
D	0015385	molecular_function	sodium:proton antiporter activity
D	0015459	molecular_function	potassium channel regulator activity
D	0015630	cellular_component	microtubule cytoskeleton
D	0019900	molecular_function	kinase binding
D	0022406	biological_process	membrane docking
D	0030133	cellular_component	transport vesicle
D	0031122	biological_process	cytoplasmic microtubule organization
D	0031397	biological_process	negative regulation of protein ubiquitination
D	0031953	biological_process	negative regulation of protein autophosphorylation
D	0032088	biological_process	negative regulation of NF-kappaB transcription factor activity
D	0032417	biological_process	positive regulation of sodium:proton antiporter activity
D	0035725	biological_process	sodium ion transmembrane transport
D	0042308	biological_process	negative regulation of protein import into nucleus
D	0045121	cellular_component	membrane raft
D	0046872	molecular_function	metal ion binding
D	0048306	molecular_function	calcium-dependent protein binding
D	0050821	biological_process	protein stabilization
D	0051222	biological_process	positive regulation of protein transport
D	0051453	biological_process	regulation of intracellular pH
D	0060050	biological_process	positive regulation of protein glycosylation
D	0061024	biological_process	membrane organization
D	0061025	biological_process	membrane fusion
D	0070062	cellular_component	extracellular exosome
D	0070885	biological_process	negative regulation of calcineurin-NFAT signaling cascade
D	0071073	biological_process	positive regulation of phospholipid biosynthetic process
D	0071468	biological_process	cellular response to acidic pH
D	0090314	biological_process	positive regulation of protein targeting to membrane
D	1990351	cellular_component	transporter complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	10
Details	binding site for residue PGT B 1001

Chain	Residue
A	LEU332
B	PGT1002
A	PGT601
B	TRP108
B	ILE109
B	MET385
B	TRP386
B	VAL389
B	LEU393
B	PHE397

---

site_id	AC2
Number of Residues	7
Details	binding site for residue PGT B 1002

Chain	Residue
B	ILE105
B	VAL160
B	PHE164
B	TYR339
B	TRP386
B	PGT1001
B	PGT1004

---

site_id	AC3
Number of Residues	9
Details	binding site for residue HG0 B 1003

Chain	Residue
A	ASP95
B	SER158
B	ASP159
B	PHE162
B	LEU163
B	ASP267
B	VAL271
B	GLU346
B	LEU468

---

site_id	AC4
Number of Residues	6
Details	binding site for residue PGT B 1004

Chain	Residue
A	ILE105
A	PGT601
A	PGT602
B	PHE335
B	TYR339
B	PGT1002

---

site_id	AC5
Number of Residues	6
Details	binding site for residue PGT A 601

Chain	Residue
A	PHE335
A	TYR339
A	PGT603
B	ILE105
B	PGT1001
B	PGT1004

---

site_id	AC6
Number of Residues	10
Details	binding site for residue PGT A 602

Chain	Residue
A	TRP108
A	ILE109
A	MET385
A	TRP386
A	VAL389
A	LEU393
A	PHE397
A	PGT603
B	LEU332
B	PGT1004

---

site_id	AC7
Number of Residues	7
Details	binding site for residue PGT A 603

Chain	Residue
A	ILE105
A	VAL160
A	PHE164
A	TYR339
A	TRP386
A	PGT601
A	PGT602

---

site_id	AC8
Number of Residues	9
Details	binding site for residue HG0 A 604

Chain	Residue
A	SER158
A	ASP159
A	PHE162
A	LEU163
A	ASP267
A	VAL271
A	GLU346
A	LEU468
B	ASP95

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	44
Details	TRANSMEM: Helical; Name=1 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	VAL99-VAL121
C	ASP123
C	ASP125
C	ASP127
C	LYS129
C	GLU134
C	ASP164
C	ASP166
C	ASP168
C	GLU175
A	VAL99-VAL121
D	ASP127
D	LYS129
D	GLU134
D	ASP164
D	ASP166
D	ASP168
D	GLU175

---

site_id	SWS_FT_FI2
Number of Residues	60
Details	TOPO_DOM: Cytoplasmic => ECO:0000305

Chain	Residue	Details
B	ILE122-PRO130
A	THR323-VAL328
A	SER372-HIS373
A	PHE439-LEU444
B	LEU177-LEU186
B	HIS250-ASN252
B	THR323-VAL328
B	SER372-HIS373
B	PHE439-LEU444
A	ILE122-PRO130
A	LEU177-LEU186
A	HIS250-ASN252

---

site_id	SWS_FT_FI3
Number of Residues	34
Details	TRANSMEM: Helical; Name=2 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	GLU131-GLY148
A	GLU131-GLY148

---

site_id	SWS_FT_FI4
Number of Residues	72
Details	TOPO_DOM: Extracellular => ECO:0000305

Chain	Residue	Details
B	VAL149-SER158
A	TYR342-LEU350
A	GLY405-ASN410
A	ASP470-PRO475
B	GLY216-ILE222
B	GLU284-GLY287
B	TYR342-LEU350
B	GLY405-ASN410
B	ASP470-PRO475
A	VAL149-SER158
A	GLY216-ILE222
A	GLU284-GLY287

---

site_id	SWS_FT_FI5
Number of Residues	34
Details	TRANSMEM: Helical; Name=3 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	ASP159-PHE176
A	ASP159-PHE176

---

site_id	SWS_FT_FI6
Number of Residues	56
Details	TRANSMEM: Helical; Name=4 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	GLY187-GLY215
A	GLY187-GLY215

---

site_id	SWS_FT_FI7
Number of Residues	52
Details	TRANSMEM: Helical; Name=5 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	GLY223-ILE249
A	GLY223-ILE249

---

site_id	SWS_FT_FI8
Number of Residues	60
Details	TRANSMEM: Helical; Name=6 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	GLU253-TYR283
A	GLU253-TYR283

---

site_id	SWS_FT_FI9
Number of Residues	68
Details	TRANSMEM: Helical; Name=7 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	ILE288-PHE322
A	ILE288-PHE322

---

site_id	SWS_FT_FI10
Number of Residues	24
Details	TRANSMEM: Helical; Name=8 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	ILE329-ALA341
A	ILE329-ALA341

---

site_id	SWS_FT_FI11
Number of Residues	40
Details	TRANSMEM: Helical; Name=9 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	SER351-ILE371
A	SER351-ILE371

---

site_id	SWS_FT_FI12
Number of Residues	60
Details	TRANSMEM: Helical; Name=10 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	LYS374-ALA404
A	LYS374-ALA404

---

site_id	SWS_FT_FI13
Number of Residues	54
Details	TRANSMEM: Helical; Name=11 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	TRP411-LYS438
A	TRP411-LYS438

---

site_id	SWS_FT_FI14
Number of Residues	48
Details	TRANSMEM: Helical; Name=12 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	THR445-LEU469
A	THR445-LEU469

---

site_id	SWS_FT_FI15
Number of Residues	58
Details	TRANSMEM: Helical; Name=13 => ECO:0000269|PubMed:34108458, ECO:0007744|PDB:7DSW

Chain	Residue	Details
B	MET476-LEU505
A	MET476-LEU505

---

site_id	SWS_FT_FI16
Number of Residues	2
Details	SITE: Channel pore-lining => ECO:0000305

Chain	Residue	Details
B	PHE161
A	PHE161

---

site_id	SWS_FT_FI17
Number of Residues	2
Details	SITE: Not glycosylated

Chain	Residue	Details
B	ASN370
A	ASN370

---