Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7C08

Crystal structure of S34Y mutant of yeast U2AF1 complex bound to 3' splice site RNA, 5'-UAGGU.

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000243	cellular_component	commitment complex
A	0000389	biological_process	mRNA 3'-splice site recognition
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0003676	molecular_function	nucleic acid binding
A	0003723	molecular_function	RNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005681	cellular_component	spliceosomal complex
A	0005829	cellular_component	cytosol
A	0006397	biological_process	mRNA processing
A	0008380	biological_process	RNA splicing
A	0030628	molecular_function	pre-mRNA 3'-splice site binding
A	0045292	biological_process	mRNA cis splicing, via spliceosome
A	0046872	molecular_function	metal ion binding
A	0071004	cellular_component	U2-type prespliceosome
A	0089701	cellular_component	U2AF complex
D	0000243	cellular_component	commitment complex
D	0000389	biological_process	mRNA 3'-splice site recognition
D	0000398	biological_process	mRNA splicing, via spliceosome
D	0003676	molecular_function	nucleic acid binding
D	0003723	molecular_function	RNA binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005681	cellular_component	spliceosomal complex
D	0005829	cellular_component	cytosol
D	0006397	biological_process	mRNA processing
D	0008380	biological_process	RNA splicing
D	0030628	molecular_function	pre-mRNA 3'-splice site binding
D	0045292	biological_process	mRNA cis splicing, via spliceosome
D	0046872	molecular_function	metal ion binding
D	0071004	cellular_component	U2-type prespliceosome
D	0089701	cellular_component	U2AF complex
G	0000243	cellular_component	commitment complex
G	0000389	biological_process	mRNA 3'-splice site recognition
G	0000398	biological_process	mRNA splicing, via spliceosome
G	0003676	molecular_function	nucleic acid binding
G	0003723	molecular_function	RNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005681	cellular_component	spliceosomal complex
G	0005829	cellular_component	cytosol
G	0006397	biological_process	mRNA processing
G	0008380	biological_process	RNA splicing
G	0030628	molecular_function	pre-mRNA 3'-splice site binding
G	0045292	biological_process	mRNA cis splicing, via spliceosome
G	0046872	molecular_function	metal ion binding
G	0071004	cellular_component	U2-type prespliceosome
G	0089701	cellular_component	U2AF complex
J	0000243	cellular_component	commitment complex
J	0000389	biological_process	mRNA 3'-splice site recognition
J	0000398	biological_process	mRNA splicing, via spliceosome
J	0003676	molecular_function	nucleic acid binding
J	0003723	molecular_function	RNA binding
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005681	cellular_component	spliceosomal complex
J	0005829	cellular_component	cytosol
J	0006397	biological_process	mRNA processing
J	0008380	biological_process	RNA splicing
J	0030628	molecular_function	pre-mRNA 3'-splice site binding
J	0045292	biological_process	mRNA cis splicing, via spliceosome
J	0046872	molecular_function	metal ion binding
J	0071004	cellular_component	U2-type prespliceosome
J	0089701	cellular_component	U2AF complex
M	0000243	cellular_component	commitment complex
M	0000389	biological_process	mRNA 3'-splice site recognition
M	0000398	biological_process	mRNA splicing, via spliceosome
M	0003676	molecular_function	nucleic acid binding
M	0003723	molecular_function	RNA binding
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005681	cellular_component	spliceosomal complex
M	0005829	cellular_component	cytosol
M	0006397	biological_process	mRNA processing
M	0008380	biological_process	RNA splicing
M	0030628	molecular_function	pre-mRNA 3'-splice site binding
M	0045292	biological_process	mRNA cis splicing, via spliceosome
M	0046872	molecular_function	metal ion binding
M	0071004	cellular_component	U2-type prespliceosome
M	0089701	cellular_component	U2AF complex
P	0000243	cellular_component	commitment complex
P	0000389	biological_process	mRNA 3'-splice site recognition
P	0000398	biological_process	mRNA splicing, via spliceosome
P	0003676	molecular_function	nucleic acid binding
P	0003723	molecular_function	RNA binding
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005681	cellular_component	spliceosomal complex
P	0005829	cellular_component	cytosol
P	0006397	biological_process	mRNA processing
P	0008380	biological_process	RNA splicing
P	0030628	molecular_function	pre-mRNA 3'-splice site binding
P	0045292	biological_process	mRNA cis splicing, via spliceosome
P	0046872	molecular_function	metal ion binding
P	0071004	cellular_component	U2-type prespliceosome
P	0089701	cellular_component	U2AF complex
S	0000243	cellular_component	commitment complex
S	0000389	biological_process	mRNA 3'-splice site recognition
S	0000398	biological_process	mRNA splicing, via spliceosome
S	0003676	molecular_function	nucleic acid binding
S	0003723	molecular_function	RNA binding
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005681	cellular_component	spliceosomal complex
S	0005829	cellular_component	cytosol
S	0006397	biological_process	mRNA processing
S	0008380	biological_process	RNA splicing
S	0030628	molecular_function	pre-mRNA 3'-splice site binding
S	0045292	biological_process	mRNA cis splicing, via spliceosome
S	0046872	molecular_function	metal ion binding
S	0071004	cellular_component	U2-type prespliceosome
S	0089701	cellular_component	U2AF complex
V	0000243	cellular_component	commitment complex
V	0000389	biological_process	mRNA 3'-splice site recognition
V	0000398	biological_process	mRNA splicing, via spliceosome
V	0003676	molecular_function	nucleic acid binding
V	0003723	molecular_function	RNA binding
V	0005515	molecular_function	protein binding
V	0005634	cellular_component	nucleus
V	0005681	cellular_component	spliceosomal complex
V	0005829	cellular_component	cytosol
V	0006397	biological_process	mRNA processing
V	0008380	biological_process	RNA splicing
V	0030628	molecular_function	pre-mRNA 3'-splice site binding
V	0045292	biological_process	mRNA cis splicing, via spliceosome
V	0046872	molecular_function	metal ion binding
V	0071004	cellular_component	U2-type prespliceosome
V	0089701	cellular_component	U2AF complex
Y	0000243	cellular_component	commitment complex
Y	0000389	biological_process	mRNA 3'-splice site recognition
Y	0000398	biological_process	mRNA splicing, via spliceosome
Y	0003676	molecular_function	nucleic acid binding
Y	0003723	molecular_function	RNA binding
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005681	cellular_component	spliceosomal complex
Y	0005829	cellular_component	cytosol
Y	0006397	biological_process	mRNA processing
Y	0008380	biological_process	RNA splicing
Y	0030628	molecular_function	pre-mRNA 3'-splice site binding
Y	0045292	biological_process	mRNA cis splicing, via spliceosome
Y	0046872	molecular_function	metal ion binding
Y	0071004	cellular_component	U2-type prespliceosome
Y	0089701	cellular_component	U2AF complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 301

Chain	Residue
A	CYS18
A	CYS27
A	CYS33
A	HIS37

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN A 302

Chain	Residue
A	CYS149
A	CYS157
A	CYS163
A	HIS167

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN D 301

Chain	Residue
D	CYS27
D	CYS33
D	HIS37
D	CYS18

site_id	AC4
Number of Residues	4
Details	binding site for residue ZN D 302

Chain	Residue
D	CYS149
D	CYS157
D	CYS163
D	HIS167

site_id	AC5
Number of Residues	4
Details	binding site for residue ZN G 301

Chain	Residue
G	CYS18
G	CYS27
G	CYS33
G	HIS37

site_id	AC6
Number of Residues	4
Details	binding site for residue ZN G 302

Chain	Residue
G	CYS149
G	CYS157
G	CYS163
G	HIS167

site_id	AC7
Number of Residues	4
Details	binding site for residue ZN J 301

Chain	Residue
J	CYS18
J	CYS27
J	CYS33
J	HIS37

site_id	AC8
Number of Residues	4
Details	binding site for residue ZN J 302

Chain	Residue
J	CYS149
J	CYS157
J	CYS163
J	HIS167

site_id	AC9
Number of Residues	4
Details	binding site for residue ZN M 301

Chain	Residue
M	CYS18
M	CYS27
M	CYS33
M	HIS37

site_id	AD1
Number of Residues	4
Details	binding site for residue ZN M 302

Chain	Residue
M	CYS149
M	CYS157
M	CYS163
M	HIS167

site_id	AD2
Number of Residues	4
Details	binding site for residue ZN P 301

Chain	Residue
P	CYS18
P	CYS27
P	CYS33
P	HIS37

site_id	AD3
Number of Residues	4
Details	binding site for residue ZN P 302

Chain	Residue
P	CYS149
P	CYS157
P	CYS163
P	HIS167

site_id	AD4
Number of Residues	4
Details	binding site for residue ZN S 301

Chain	Residue
S	CYS18
S	CYS27
S	CYS33
S	HIS37

site_id	AD5
Number of Residues	4
Details	binding site for residue ZN S 302

Chain	Residue
S	CYS149
S	CYS157
S	CYS163
S	HIS167

site_id	AD6
Number of Residues	4
Details	binding site for residue ZN V 301

Chain	Residue
V	CYS18
V	CYS27
V	CYS33
V	HIS37

site_id	AD7
Number of Residues	4
Details	binding site for residue ZN V 302

Chain	Residue
V	CYS149
V	CYS157
V	CYS163
V	HIS167

site_id	AD8
Number of Residues	4
Details	binding site for residue ZN Y 301

Chain	Residue
Y	CYS18
Y	CYS27
Y	CYS33
Y	HIS37

site_id	AD9
Number of Residues	4
Details	binding site for residue ZN Y 302

Chain	Residue
Y	CYS149
Y	CYS157
Y	CYS163
Y	HIS167

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	252
Details	ZN_FING: C3H1-type 1 => ECO:0000255\|PROSITE-ProRule:PRU00723

Chain	Residue	Details
A	GLU12-PRO40
D	GLU12-PRO40
G	GLU12-PRO40
J	GLU12-PRO40
M	GLU12-PRO40
P	GLU12-PRO40
S	GLU12-PRO40
V	GLU12-PRO40
Y	GLU12-PRO40

site_id	SWS_FT_FI2
Number of Residues	243
Details	ZN_FING: C3H1-type 2 => ECO:0000255\|PROSITE-ProRule:PRU00723

Chain	Residue	Details
A	ASP143-LYS170
D	ASP143-LYS170
G	ASP143-LYS170
J	ASP143-LYS170
M	ASP143-LYS170
P	ASP143-LYS170
S	ASP143-LYS170
V	ASP143-LYS170
Y	ASP143-LYS170

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)