7C08
Crystal structure of S34Y mutant of yeast U2AF1 complex bound to 3' splice site RNA, 5'-UAGGU.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.506, 259.000, 94.822 |
| Unit cell angles | 90.00, 100.68, 90.00 |
Refinement procedure
| Resolution | 46.590 - 3.350 |
| R-factor | 0.2162 |
| Rwork | 0.214 |
| R-free | 0.25230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yh8 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.500 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.400 | 3.430 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.230 | 1.158 |
| Number of reflections | 64167 | 4510 |
| <I/σ(I)> | 8.3 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.1 |
| CC(1/2) | 0.971 | 0.742 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M NaCitrate, 10% PEG 35000 |
