Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

7B9K

Cryo-EM structure of the dihydrolipoyl transacetylase cubic core of the E. coli pyruvate dehydrogenase complex including lipoyl domains

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004738	molecular_function	pyruvate dehydrogenase activity
A	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
A	0006090	biological_process	pyruvate metabolic process
A	0006096	biological_process	glycolytic process
A	0016407	molecular_function	acetyltransferase activity
A	0016746	molecular_function	acyltransferase activity
A	0031405	molecular_function	lipoic acid binding
A	0042867	biological_process	pyruvate catabolic process
A	0045254	cellular_component	pyruvate dehydrogenase complex
B	0004738	molecular_function	pyruvate dehydrogenase activity
B	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
B	0006090	biological_process	pyruvate metabolic process
B	0006096	biological_process	glycolytic process
B	0016407	molecular_function	acetyltransferase activity
B	0016746	molecular_function	acyltransferase activity
B	0031405	molecular_function	lipoic acid binding
B	0042867	biological_process	pyruvate catabolic process
B	0045254	cellular_component	pyruvate dehydrogenase complex
C	0004738	molecular_function	pyruvate dehydrogenase activity
C	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
C	0006090	biological_process	pyruvate metabolic process
C	0006096	biological_process	glycolytic process
C	0016407	molecular_function	acetyltransferase activity
C	0016746	molecular_function	acyltransferase activity
C	0031405	molecular_function	lipoic acid binding
C	0042867	biological_process	pyruvate catabolic process
C	0045254	cellular_component	pyruvate dehydrogenase complex
D	0004738	molecular_function	pyruvate dehydrogenase activity
D	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
D	0006090	biological_process	pyruvate metabolic process
D	0006096	biological_process	glycolytic process
D	0016407	molecular_function	acetyltransferase activity
D	0016746	molecular_function	acyltransferase activity
D	0031405	molecular_function	lipoic acid binding
D	0042867	biological_process	pyruvate catabolic process
D	0045254	cellular_component	pyruvate dehydrogenase complex
E	0004738	molecular_function	pyruvate dehydrogenase activity
E	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
E	0006090	biological_process	pyruvate metabolic process
E	0006096	biological_process	glycolytic process
E	0016407	molecular_function	acetyltransferase activity
E	0016746	molecular_function	acyltransferase activity
E	0031405	molecular_function	lipoic acid binding
E	0042867	biological_process	pyruvate catabolic process
E	0045254	cellular_component	pyruvate dehydrogenase complex
F	0004738	molecular_function	pyruvate dehydrogenase activity
F	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
F	0006090	biological_process	pyruvate metabolic process
F	0006096	biological_process	glycolytic process
F	0016407	molecular_function	acetyltransferase activity
F	0016746	molecular_function	acyltransferase activity
F	0031405	molecular_function	lipoic acid binding
F	0042867	biological_process	pyruvate catabolic process
F	0045254	cellular_component	pyruvate dehydrogenase complex
G	0004738	molecular_function	pyruvate dehydrogenase activity
G	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
G	0006090	biological_process	pyruvate metabolic process
G	0006096	biological_process	glycolytic process
G	0016407	molecular_function	acetyltransferase activity
G	0016746	molecular_function	acyltransferase activity
G	0031405	molecular_function	lipoic acid binding
G	0042867	biological_process	pyruvate catabolic process
G	0045254	cellular_component	pyruvate dehydrogenase complex
H	0004738	molecular_function	pyruvate dehydrogenase activity
H	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
H	0006090	biological_process	pyruvate metabolic process
H	0006096	biological_process	glycolytic process
H	0016407	molecular_function	acetyltransferase activity
H	0016746	molecular_function	acyltransferase activity
H	0031405	molecular_function	lipoic acid binding
H	0042867	biological_process	pyruvate catabolic process
H	0045254	cellular_component	pyruvate dehydrogenase complex
I	0004738	molecular_function	pyruvate dehydrogenase activity
I	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
I	0006090	biological_process	pyruvate metabolic process
I	0006096	biological_process	glycolytic process
I	0016407	molecular_function	acetyltransferase activity
I	0016746	molecular_function	acyltransferase activity
I	0031405	molecular_function	lipoic acid binding
I	0042867	biological_process	pyruvate catabolic process
I	0045254	cellular_component	pyruvate dehydrogenase complex
J	0004738	molecular_function	pyruvate dehydrogenase activity
J	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
J	0006090	biological_process	pyruvate metabolic process
J	0006096	biological_process	glycolytic process
J	0016407	molecular_function	acetyltransferase activity
J	0016746	molecular_function	acyltransferase activity
J	0031405	molecular_function	lipoic acid binding
J	0042867	biological_process	pyruvate catabolic process
J	0045254	cellular_component	pyruvate dehydrogenase complex
K	0004738	molecular_function	pyruvate dehydrogenase activity
K	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
K	0006090	biological_process	pyruvate metabolic process
K	0006096	biological_process	glycolytic process
K	0016407	molecular_function	acetyltransferase activity
K	0016746	molecular_function	acyltransferase activity
K	0031405	molecular_function	lipoic acid binding
K	0042867	biological_process	pyruvate catabolic process
K	0045254	cellular_component	pyruvate dehydrogenase complex
L	0004738	molecular_function	pyruvate dehydrogenase activity
L	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
L	0006090	biological_process	pyruvate metabolic process
L	0006096	biological_process	glycolytic process
L	0016407	molecular_function	acetyltransferase activity
L	0016746	molecular_function	acyltransferase activity
L	0031405	molecular_function	lipoic acid binding
L	0042867	biological_process	pyruvate catabolic process
L	0045254	cellular_component	pyruvate dehydrogenase complex
M	0004738	molecular_function	pyruvate dehydrogenase activity
M	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
M	0005515	molecular_function	protein binding
M	0005737	cellular_component	cytoplasm
M	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
M	0006090	biological_process	pyruvate metabolic process
M	0006096	biological_process	glycolytic process
M	0016407	molecular_function	acetyltransferase activity
M	0016746	molecular_function	acyltransferase activity
M	0031405	molecular_function	lipoic acid binding
M	0042867	biological_process	pyruvate catabolic process
M	0045254	cellular_component	pyruvate dehydrogenase complex
N	0004738	molecular_function	pyruvate dehydrogenase activity
N	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
N	0005515	molecular_function	protein binding
N	0005737	cellular_component	cytoplasm
N	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
N	0006090	biological_process	pyruvate metabolic process
N	0006096	biological_process	glycolytic process
N	0016407	molecular_function	acetyltransferase activity
N	0016746	molecular_function	acyltransferase activity
N	0031405	molecular_function	lipoic acid binding
N	0042867	biological_process	pyruvate catabolic process
N	0045254	cellular_component	pyruvate dehydrogenase complex
O	0004738	molecular_function	pyruvate dehydrogenase activity
O	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
O	0005515	molecular_function	protein binding
O	0005737	cellular_component	cytoplasm
O	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
O	0006090	biological_process	pyruvate metabolic process
O	0006096	biological_process	glycolytic process
O	0016407	molecular_function	acetyltransferase activity
O	0016746	molecular_function	acyltransferase activity
O	0031405	molecular_function	lipoic acid binding
O	0042867	biological_process	pyruvate catabolic process
O	0045254	cellular_component	pyruvate dehydrogenase complex
P	0004738	molecular_function	pyruvate dehydrogenase activity
P	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
P	0005515	molecular_function	protein binding
P	0005737	cellular_component	cytoplasm
P	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
P	0006090	biological_process	pyruvate metabolic process
P	0006096	biological_process	glycolytic process
P	0016407	molecular_function	acetyltransferase activity
P	0016746	molecular_function	acyltransferase activity
P	0031405	molecular_function	lipoic acid binding
P	0042867	biological_process	pyruvate catabolic process
P	0045254	cellular_component	pyruvate dehydrogenase complex
Q	0004738	molecular_function	pyruvate dehydrogenase activity
Q	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
Q	0005515	molecular_function	protein binding
Q	0005737	cellular_component	cytoplasm
Q	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
Q	0006090	biological_process	pyruvate metabolic process
Q	0006096	biological_process	glycolytic process
Q	0016407	molecular_function	acetyltransferase activity
Q	0016746	molecular_function	acyltransferase activity
Q	0031405	molecular_function	lipoic acid binding
Q	0042867	biological_process	pyruvate catabolic process
Q	0045254	cellular_component	pyruvate dehydrogenase complex
R	0004738	molecular_function	pyruvate dehydrogenase activity
R	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
R	0005515	molecular_function	protein binding
R	0005737	cellular_component	cytoplasm
R	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
R	0006090	biological_process	pyruvate metabolic process
R	0006096	biological_process	glycolytic process
R	0016407	molecular_function	acetyltransferase activity
R	0016746	molecular_function	acyltransferase activity
R	0031405	molecular_function	lipoic acid binding
R	0042867	biological_process	pyruvate catabolic process
R	0045254	cellular_component	pyruvate dehydrogenase complex
S	0004738	molecular_function	pyruvate dehydrogenase activity
S	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
S	0005515	molecular_function	protein binding
S	0005737	cellular_component	cytoplasm
S	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
S	0006090	biological_process	pyruvate metabolic process
S	0006096	biological_process	glycolytic process
S	0016407	molecular_function	acetyltransferase activity
S	0016746	molecular_function	acyltransferase activity
S	0031405	molecular_function	lipoic acid binding
S	0042867	biological_process	pyruvate catabolic process
S	0045254	cellular_component	pyruvate dehydrogenase complex
T	0004738	molecular_function	pyruvate dehydrogenase activity
T	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
T	0005515	molecular_function	protein binding
T	0005737	cellular_component	cytoplasm
T	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
T	0006090	biological_process	pyruvate metabolic process
T	0006096	biological_process	glycolytic process
T	0016407	molecular_function	acetyltransferase activity
T	0016746	molecular_function	acyltransferase activity
T	0031405	molecular_function	lipoic acid binding
T	0042867	biological_process	pyruvate catabolic process
T	0045254	cellular_component	pyruvate dehydrogenase complex
U	0004738	molecular_function	pyruvate dehydrogenase activity
U	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
U	0005515	molecular_function	protein binding
U	0005737	cellular_component	cytoplasm
U	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
U	0006090	biological_process	pyruvate metabolic process
U	0006096	biological_process	glycolytic process
U	0016407	molecular_function	acetyltransferase activity
U	0016746	molecular_function	acyltransferase activity
U	0031405	molecular_function	lipoic acid binding
U	0042867	biological_process	pyruvate catabolic process
U	0045254	cellular_component	pyruvate dehydrogenase complex
V	0004738	molecular_function	pyruvate dehydrogenase activity
V	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
V	0005515	molecular_function	protein binding
V	0005737	cellular_component	cytoplasm
V	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
V	0006090	biological_process	pyruvate metabolic process
V	0006096	biological_process	glycolytic process
V	0016407	molecular_function	acetyltransferase activity
V	0016746	molecular_function	acyltransferase activity
V	0031405	molecular_function	lipoic acid binding
V	0042867	biological_process	pyruvate catabolic process
V	0045254	cellular_component	pyruvate dehydrogenase complex
W	0004738	molecular_function	pyruvate dehydrogenase activity
W	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
W	0005515	molecular_function	protein binding
W	0005737	cellular_component	cytoplasm
W	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
W	0006090	biological_process	pyruvate metabolic process
W	0006096	biological_process	glycolytic process
W	0016407	molecular_function	acetyltransferase activity
W	0016746	molecular_function	acyltransferase activity
W	0031405	molecular_function	lipoic acid binding
W	0042867	biological_process	pyruvate catabolic process
W	0045254	cellular_component	pyruvate dehydrogenase complex
X	0004738	molecular_function	pyruvate dehydrogenase activity
X	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
X	0005515	molecular_function	protein binding
X	0005737	cellular_component	cytoplasm
X	0006086	biological_process	acetyl-CoA biosynthetic process from pyruvate
X	0006090	biological_process	pyruvate metabolic process
X	0006096	biological_process	glycolytic process
X	0016407	molecular_function	acetyltransferase activity
X	0016746	molecular_function	acyltransferase activity
X	0031405	molecular_function	lipoic acid binding
X	0042867	biological_process	pyruvate catabolic process
X	0045254	cellular_component	pyruvate dehydrogenase complex

Functional Information from PROSITE/UniProt

site_id	PS00189
Number of Residues	30
Details	LIPOYL 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. GdkVeaeQSLitVEGdKASmeVpspqaGiV

Chain	Residue	Details
A	GLY25-VAL54
A	GLY128-VAL157
A	GLY229-VAL258

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	48
Details	ACT_SITE: ACT_SITE => ECO:0000255

Chain	Residue	Details
A	CYS547
E	ASP607
F	CYS547
F	ASP607
G	CYS547
G	ASP607
H	CYS547
H	ASP607
I	CYS547
I	ASP607
J	CYS547
A	ASP607
J	ASP607
K	CYS547
K	ASP607
L	CYS547
L	ASP607
M	CYS547
M	ASP607
N	CYS547
N	ASP607
O	CYS547
B	CYS547
O	ASP607
P	CYS547
P	ASP607
Q	CYS547
Q	ASP607
R	CYS547
R	ASP607
S	CYS547
S	ASP607
T	CYS547
B	ASP607
T	ASP607
U	CYS547
U	ASP607
V	CYS547
V	ASP607
W	CYS547
W	ASP607
X	CYS547
X	ASP607
C	CYS547
C	ASP607
D	CYS547
D	ASP607
E	CYS547

site_id	SWS_FT_FI2
Number of Residues	24
Details	ACT_SITE:

Chain	Residue	Details
A	HIS603
J	HIS603
K	HIS603
L	HIS603
M	HIS603
N	HIS603
O	HIS603
P	HIS603
Q	HIS603
R	HIS603
S	HIS603
B	HIS603
T	HIS603
U	HIS603
V	HIS603
W	HIS603
X	HIS603
C	HIS603
D	HIS603
E	HIS603
F	HIS603
G	HIS603
H	HIS603
I	HIS603

site_id	SWS_FT_FI3
Number of Residues	24
Details	MOD_RES: N6-lipoyllysine => ECO:0000255\|PROSITE-ProRule:PRU01066, ECO:0000269\|PubMed:6345153, ECO:0000269\|PubMed:6821375

Chain	Residue	Details
A	LYS41
J	LYS41
K	LYS41
L	LYS41
M	LYS41
N	LYS41
O	LYS41
P	LYS41
Q	LYS41
R	LYS41
S	LYS41
B	LYS41
T	LYS41
U	LYS41
V	LYS41
W	LYS41
X	LYS41
C	LYS41
D	LYS41
E	LYS41
F	LYS41
G	LYS41
H	LYS41
I	LYS41

site_id	SWS_FT_FI4
Number of Residues	24
Details	MOD_RES: N6-lipoyllysine => ECO:0000255\|PROSITE-ProRule:PRU01066, ECO:0000269\|PubMed:2121129, ECO:0000269\|PubMed:6345153

Chain	Residue	Details
A	LYS144
J	LYS144
K	LYS144
L	LYS144
M	LYS144
N	LYS144
O	LYS144
P	LYS144
Q	LYS144
R	LYS144
S	LYS144
B	LYS144
T	LYS144
U	LYS144
V	LYS144
W	LYS144
X	LYS144
C	LYS144
D	LYS144
E	LYS144
F	LYS144
G	LYS144
H	LYS144
I	LYS144

site_id	SWS_FT_FI5
Number of Residues	24
Details	MOD_RES: N6-lipoyllysine => ECO:0000255\|PROSITE-ProRule:PRU01066, ECO:0000269\|PubMed:6345153

Chain	Residue	Details
A	LA2245
J	LA2245
K	LA2245
L	LA2245
M	LA2245
N	LA2245
O	LA2245
P	LA2245
Q	LA2245
R	LA2245
S	LA2245
B	LA2245
T	LA2245
U	LA2245
V	LA2245
W	LA2245
X	LA2245
C	LA2245
D	LA2245
E	LA2245
F	LA2245
G	LA2245
H	LA2245
I	LA2245

site_id	SWS_FT_FI6
Number of Residues	24
Details	MOD_RES: N6-acetyllysine => ECO:0000269\|PubMed:18723842

Chain	Residue	Details
A	LYS396
J	LYS396
K	LYS396
L	LYS396
M	LYS396
N	LYS396
O	LYS396
P	LYS396
Q	LYS396
R	LYS396
S	LYS396
B	LYS396
T	LYS396
U	LYS396
V	LYS396
W	LYS396
X	LYS396
C	LYS396
D	LYS396
E	LYS396
F	LYS396
G	LYS396
H	LYS396
I	LYS396

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)