7B9K

Cryo-EM structure of the dihydrolipoyl transacetylase cubic core of the E. coli pyruvate dehydrogenase complex including lipoyl domains

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
A	0005515	molecular_function	protein binding
A	0005737	cellular_component	cytoplasm
A	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
A	0031405	molecular_function	lipoic acid binding
A	0042867	biological_process	pyruvate catabolic process
A	0045254	cellular_component	pyruvate dehydrogenase complex
A	0097532	biological_process	stress response to acid chemical
B	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
B	0016740	molecular_function	transferase activity
B	0016746	molecular_function	acyltransferase activity
B	0031405	molecular_function	lipoic acid binding
B	0042867	biological_process	pyruvate catabolic process
B	0045254	cellular_component	pyruvate dehydrogenase complex
B	0097532	biological_process	stress response to acid chemical
C	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
C	0005515	molecular_function	protein binding
C	0005737	cellular_component	cytoplasm
C	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
C	0031405	molecular_function	lipoic acid binding
C	0042867	biological_process	pyruvate catabolic process
C	0045254	cellular_component	pyruvate dehydrogenase complex
C	0097532	biological_process	stress response to acid chemical
D	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
D	0016740	molecular_function	transferase activity
D	0016746	molecular_function	acyltransferase activity
D	0031405	molecular_function	lipoic acid binding
D	0042867	biological_process	pyruvate catabolic process
D	0045254	cellular_component	pyruvate dehydrogenase complex
D	0097532	biological_process	stress response to acid chemical
E	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
E	0005515	molecular_function	protein binding
E	0005737	cellular_component	cytoplasm
E	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
E	0016740	molecular_function	transferase activity
E	0016746	molecular_function	acyltransferase activity
E	0031405	molecular_function	lipoic acid binding
E	0042867	biological_process	pyruvate catabolic process
E	0045254	cellular_component	pyruvate dehydrogenase complex
E	0097532	biological_process	stress response to acid chemical
F	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
F	0005515	molecular_function	protein binding
F	0005737	cellular_component	cytoplasm
F	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
F	0016740	molecular_function	transferase activity
F	0016746	molecular_function	acyltransferase activity
F	0031405	molecular_function	lipoic acid binding
F	0042867	biological_process	pyruvate catabolic process
F	0045254	cellular_component	pyruvate dehydrogenase complex
F	0097532	biological_process	stress response to acid chemical
G	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
G	0005515	molecular_function	protein binding
G	0005737	cellular_component	cytoplasm
G	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
G	0016740	molecular_function	transferase activity
G	0016746	molecular_function	acyltransferase activity
G	0031405	molecular_function	lipoic acid binding
G	0042867	biological_process	pyruvate catabolic process
G	0045254	cellular_component	pyruvate dehydrogenase complex
G	0097532	biological_process	stress response to acid chemical
H	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
H	0016740	molecular_function	transferase activity
H	0016746	molecular_function	acyltransferase activity
H	0031405	molecular_function	lipoic acid binding
H	0042867	biological_process	pyruvate catabolic process
H	0045254	cellular_component	pyruvate dehydrogenase complex
H	0097532	biological_process	stress response to acid chemical
I	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
I	0005515	molecular_function	protein binding
I	0005737	cellular_component	cytoplasm
I	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
I	0016740	molecular_function	transferase activity
I	0016746	molecular_function	acyltransferase activity
I	0031405	molecular_function	lipoic acid binding
I	0042867	biological_process	pyruvate catabolic process
I	0045254	cellular_component	pyruvate dehydrogenase complex
I	0097532	biological_process	stress response to acid chemical
J	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
J	0005515	molecular_function	protein binding
J	0005737	cellular_component	cytoplasm
J	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
J	0016740	molecular_function	transferase activity
J	0016746	molecular_function	acyltransferase activity
J	0031405	molecular_function	lipoic acid binding
J	0042867	biological_process	pyruvate catabolic process
J	0045254	cellular_component	pyruvate dehydrogenase complex
J	0097532	biological_process	stress response to acid chemical
K	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
K	0005515	molecular_function	protein binding
K	0005737	cellular_component	cytoplasm
K	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
K	0016740	molecular_function	transferase activity
K	0016746	molecular_function	acyltransferase activity
K	0031405	molecular_function	lipoic acid binding
K	0042867	biological_process	pyruvate catabolic process
K	0045254	cellular_component	pyruvate dehydrogenase complex
K	0097532	biological_process	stress response to acid chemical
L	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
L	0005515	molecular_function	protein binding
L	0005737	cellular_component	cytoplasm
L	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
L	0016740	molecular_function	transferase activity
L	0016746	molecular_function	acyltransferase activity
L	0031405	molecular_function	lipoic acid binding
L	0042867	biological_process	pyruvate catabolic process
L	0045254	cellular_component	pyruvate dehydrogenase complex
L	0097532	biological_process	stress response to acid chemical
M	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
M	0005515	molecular_function	protein binding
M	0005737	cellular_component	cytoplasm
M	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
M	0016740	molecular_function	transferase activity
M	0016746	molecular_function	acyltransferase activity
M	0031405	molecular_function	lipoic acid binding
M	0042867	biological_process	pyruvate catabolic process
M	0045254	cellular_component	pyruvate dehydrogenase complex
M	0097532	biological_process	stress response to acid chemical
N	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
N	0005515	molecular_function	protein binding
N	0005737	cellular_component	cytoplasm
N	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
N	0016740	molecular_function	transferase activity
N	0016746	molecular_function	acyltransferase activity
N	0031405	molecular_function	lipoic acid binding
N	0042867	biological_process	pyruvate catabolic process
N	0045254	cellular_component	pyruvate dehydrogenase complex
N	0097532	biological_process	stress response to acid chemical
O	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
O	0005515	molecular_function	protein binding
O	0005737	cellular_component	cytoplasm
O	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
O	0016740	molecular_function	transferase activity
O	0016746	molecular_function	acyltransferase activity
O	0031405	molecular_function	lipoic acid binding
O	0042867	biological_process	pyruvate catabolic process
O	0045254	cellular_component	pyruvate dehydrogenase complex
O	0097532	biological_process	stress response to acid chemical
P	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
P	0005515	molecular_function	protein binding
P	0005737	cellular_component	cytoplasm
P	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
P	0016740	molecular_function	transferase activity
P	0016746	molecular_function	acyltransferase activity
P	0031405	molecular_function	lipoic acid binding
P	0042867	biological_process	pyruvate catabolic process
P	0045254	cellular_component	pyruvate dehydrogenase complex
P	0097532	biological_process	stress response to acid chemical
Q	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
Q	0005515	molecular_function	protein binding
Q	0005737	cellular_component	cytoplasm
Q	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
Q	0016740	molecular_function	transferase activity
Q	0016746	molecular_function	acyltransferase activity
Q	0031405	molecular_function	lipoic acid binding
Q	0042867	biological_process	pyruvate catabolic process
Q	0045254	cellular_component	pyruvate dehydrogenase complex
Q	0097532	biological_process	stress response to acid chemical
R	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
R	0005515	molecular_function	protein binding
R	0005737	cellular_component	cytoplasm
R	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
R	0016740	molecular_function	transferase activity
R	0016746	molecular_function	acyltransferase activity
R	0031405	molecular_function	lipoic acid binding
R	0042867	biological_process	pyruvate catabolic process
R	0045254	cellular_component	pyruvate dehydrogenase complex
R	0097532	biological_process	stress response to acid chemical
S	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
S	0005515	molecular_function	protein binding
S	0005737	cellular_component	cytoplasm
S	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
S	0016740	molecular_function	transferase activity
S	0016746	molecular_function	acyltransferase activity
S	0031405	molecular_function	lipoic acid binding
S	0042867	biological_process	pyruvate catabolic process
S	0045254	cellular_component	pyruvate dehydrogenase complex
S	0097532	biological_process	stress response to acid chemical
T	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
T	0005515	molecular_function	protein binding
T	0005737	cellular_component	cytoplasm
T	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
T	0016740	molecular_function	transferase activity
T	0016746	molecular_function	acyltransferase activity
T	0031405	molecular_function	lipoic acid binding
T	0042867	biological_process	pyruvate catabolic process
T	0045254	cellular_component	pyruvate dehydrogenase complex
T	0097532	biological_process	stress response to acid chemical
U	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
U	0005515	molecular_function	protein binding
U	0005737	cellular_component	cytoplasm
U	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
U	0016740	molecular_function	transferase activity
U	0016746	molecular_function	acyltransferase activity
U	0031405	molecular_function	lipoic acid binding
U	0042867	biological_process	pyruvate catabolic process
U	0045254	cellular_component	pyruvate dehydrogenase complex
U	0097532	biological_process	stress response to acid chemical
V	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
V	0005515	molecular_function	protein binding
V	0005737	cellular_component	cytoplasm
V	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
V	0016740	molecular_function	transferase activity
V	0016746	molecular_function	acyltransferase activity
V	0031405	molecular_function	lipoic acid binding
V	0042867	biological_process	pyruvate catabolic process
V	0045254	cellular_component	pyruvate dehydrogenase complex
V	0097532	biological_process	stress response to acid chemical
W	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
W	0005515	molecular_function	protein binding
W	0005737	cellular_component	cytoplasm
W	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
W	0016740	molecular_function	transferase activity
W	0016746	molecular_function	acyltransferase activity
W	0031405	molecular_function	lipoic acid binding
W	0042867	biological_process	pyruvate catabolic process
W	0045254	cellular_component	pyruvate dehydrogenase complex
W	0097532	biological_process	stress response to acid chemical
X	0004742	molecular_function	dihydrolipoyllysine-residue acetyltransferase activity
X	0005515	molecular_function	protein binding
X	0005737	cellular_component	cytoplasm
X	0006086	biological_process	pyruvate decarboxylation to acetyl-CoA
X	0016740	molecular_function	transferase activity
X	0016746	molecular_function	acyltransferase activity
X	0031405	molecular_function	lipoic acid binding
X	0042867	biological_process	pyruvate catabolic process
X	0045254	cellular_component	pyruvate dehydrogenase complex
X	0097532	biological_process	stress response to acid chemical

Functional Information from PROSITE/UniProt

site_id	PS00189
Number of Residues	30
Details	LIPOYL 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. GdkVeaeQSLitVEGdKASmeVpspqaGiV

Chain	Residue	Details
A	GLY25-VAL54
A	GLY128-VAL157
A	GLY229-VAL258

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	600
Details	Region: {"description":"Hydrophobic"}

Chain

Residue

Details

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site_id	SWS_FT_FI2
Number of Residues	48
Details	Active site: {"evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	24
Details	Active site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	24
Details	Modified residue: {"description":"N6-lipoyllysine","evidences":[{"source":"PROSITE-ProRule","id":"PRU01066","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"6345153","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	24
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"18723842","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---