7B12

HUMAN IMMUNOPROTEASOME 20S PARTICLE IN COMPLEX WITH [2-(3-ethylphenyl)-1-[(2S)-3-phenyl-2-[(pyrazin-2-yl)formamido]propanamido]ethyl]boronic acid

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0005839	cellular_component	proteasome core complex
1	0051603	biological_process	proteolysis involved in protein catabolic process
2	0005839	cellular_component	proteasome core complex
2	0051603	biological_process	proteolysis involved in protein catabolic process
A	0000502	cellular_component	proteasome complex
A	0000932	cellular_component	P-body
A	0003723	molecular_function	RNA binding
A	0004175	molecular_function	endopeptidase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005839	cellular_component	proteasome core complex
A	0005840	cellular_component	ribosome
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0016363	cellular_component	nuclear matrix
A	0019773	cellular_component	proteasome core complex, alpha-subunit complex
A	0030016	cellular_component	myofibril
A	0030017	cellular_component	sarcomere
A	0035639	molecular_function	purine ribonucleoside triphosphate binding
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0050727	biological_process	regulation of inflammatory response
A	0051059	molecular_function	NF-kappaB binding
A	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
A	0051603	biological_process	proteolysis involved in protein catabolic process
A	0070062	cellular_component	extracellular exosome
B	0000502	cellular_component	proteasome complex
B	0000932	cellular_component	P-body
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005839	cellular_component	proteasome core complex
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0009615	biological_process	response to virus
B	0019773	cellular_component	proteasome core complex, alpha-subunit complex
B	0034774	cellular_component	secretory granule lumen
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0051603	biological_process	proteolysis involved in protein catabolic process
B	0070062	cellular_component	extracellular exosome
B	1904813	cellular_component	ficolin-1-rich granule lumen
C	0000502	cellular_component	proteasome complex
C	0000932	cellular_component	P-body
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005839	cellular_component	proteasome core complex
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0019773	cellular_component	proteasome core complex, alpha-subunit complex
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0043231	cellular_component	intracellular membrane-bounded organelle
C	0051603	biological_process	proteolysis involved in protein catabolic process
C	0070062	cellular_component	extracellular exosome
D	0000502	cellular_component	proteasome complex
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005839	cellular_component	proteasome core complex
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0019773	cellular_component	proteasome core complex, alpha-subunit complex
D	0042802	molecular_function	identical protein binding
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0051603	biological_process	proteolysis involved in protein catabolic process
D	0070062	cellular_component	extracellular exosome
D	0098794	cellular_component	postsynapse
E	0000502	cellular_component	proteasome complex
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005839	cellular_component	proteasome core complex
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0019773	cellular_component	proteasome core complex, alpha-subunit complex
E	0034774	cellular_component	secretory granule lumen
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0051603	biological_process	proteolysis involved in protein catabolic process
E	0070062	cellular_component	extracellular exosome
E	1904813	cellular_component	ficolin-1-rich granule lumen
F	0000502	cellular_component	proteasome complex
F	0001530	molecular_function	lipopolysaccharide binding
F	0002376	biological_process	immune system process
F	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005813	cellular_component	centrosome
F	0005829	cellular_component	cytosol
F	0005839	cellular_component	proteasome core complex
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0019773	cellular_component	proteasome core complex, alpha-subunit complex
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0051603	biological_process	proteolysis involved in protein catabolic process
F	0070062	cellular_component	extracellular exosome
G	0000502	cellular_component	proteasome complex
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005839	cellular_component	proteasome core complex
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0019773	cellular_component	proteasome core complex, alpha-subunit complex
G	0031625	molecular_function	ubiquitin protein ligase binding
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0045202	cellular_component	synapse
G	0051603	biological_process	proteolysis involved in protein catabolic process
G	0052548	biological_process	regulation of endopeptidase activity
G	0070062	cellular_component	extracellular exosome
H	0000502	cellular_component	proteasome complex
H	0002376	biological_process	immune system process
H	0004175	molecular_function	endopeptidase activity
H	0004298	molecular_function	threonine-type endopeptidase activity
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005839	cellular_component	proteasome core complex
H	0006508	biological_process	proteolysis
H	0019774	cellular_component	proteasome core complex, beta-subunit complex
H	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
H	0051603	biological_process	proteolysis involved in protein catabolic process
H	0070062	cellular_component	extracellular exosome
H	1990111	cellular_component	spermatoproteasome complex
H	2000116	biological_process	regulation of cysteine-type endopeptidase activity
i	0004298	molecular_function	threonine-type endopeptidase activity
i	0005839	cellular_component	proteasome core complex
i	0051603	biological_process	proteolysis involved in protein catabolic process
J	0000502	cellular_component	proteasome complex
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005839	cellular_component	proteasome core complex
J	0019774	cellular_component	proteasome core complex, beta-subunit complex
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0051603	biological_process	proteolysis involved in protein catabolic process
J	0070062	cellular_component	extracellular exosome
K	0000502	cellular_component	proteasome complex
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005839	cellular_component	proteasome core complex
K	0010243	biological_process	response to organonitrogen compound
K	0010498	biological_process	proteasomal protein catabolic process
K	0014070	biological_process	response to organic cyclic compound
K	0016020	cellular_component	membrane
K	0019774	cellular_component	proteasome core complex, beta-subunit complex
K	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
K	0051603	biological_process	proteolysis involved in protein catabolic process
K	0070062	cellular_component	extracellular exosome
L	0004298	molecular_function	threonine-type endopeptidase activity
L	0005839	cellular_component	proteasome core complex
L	0051603	biological_process	proteolysis involved in protein catabolic process
M	0005839	cellular_component	proteasome core complex
M	0051603	biological_process	proteolysis involved in protein catabolic process
N	0005839	cellular_component	proteasome core complex
N	0051603	biological_process	proteolysis involved in protein catabolic process
O	0000502	cellular_component	proteasome complex
O	0000932	cellular_component	P-body
O	0003723	molecular_function	RNA binding
O	0004175	molecular_function	endopeptidase activity
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005737	cellular_component	cytoplasm
O	0005829	cellular_component	cytosol
O	0005839	cellular_component	proteasome core complex
O	0005840	cellular_component	ribosome
O	0006511	biological_process	ubiquitin-dependent protein catabolic process
O	0016363	cellular_component	nuclear matrix
O	0019773	cellular_component	proteasome core complex, alpha-subunit complex
O	0030016	cellular_component	myofibril
O	0030017	cellular_component	sarcomere
O	0035639	molecular_function	purine ribonucleoside triphosphate binding
O	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
O	0050727	biological_process	regulation of inflammatory response
O	0051059	molecular_function	NF-kappaB binding
O	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
O	0051603	biological_process	proteolysis involved in protein catabolic process
O	0070062	cellular_component	extracellular exosome
P	0000502	cellular_component	proteasome complex
P	0000932	cellular_component	P-body
P	0005515	molecular_function	protein binding
P	0005576	cellular_component	extracellular region
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0005839	cellular_component	proteasome core complex
P	0006511	biological_process	ubiquitin-dependent protein catabolic process
P	0009615	biological_process	response to virus
P	0019773	cellular_component	proteasome core complex, alpha-subunit complex
P	0034774	cellular_component	secretory granule lumen
P	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
P	0051603	biological_process	proteolysis involved in protein catabolic process
P	0070062	cellular_component	extracellular exosome
P	1904813	cellular_component	ficolin-1-rich granule lumen
Q	0000502	cellular_component	proteasome complex
Q	0000932	cellular_component	P-body
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005839	cellular_component	proteasome core complex
Q	0006511	biological_process	ubiquitin-dependent protein catabolic process
Q	0019773	cellular_component	proteasome core complex, alpha-subunit complex
Q	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Q	0043231	cellular_component	intracellular membrane-bounded organelle
Q	0051603	biological_process	proteolysis involved in protein catabolic process
Q	0070062	cellular_component	extracellular exosome
r	0000502	cellular_component	proteasome complex
r	0005515	molecular_function	protein binding
r	0005634	cellular_component	nucleus
r	0005654	cellular_component	nucleoplasm
r	0005737	cellular_component	cytoplasm
r	0005829	cellular_component	cytosol
r	0005839	cellular_component	proteasome core complex
r	0006511	biological_process	ubiquitin-dependent protein catabolic process
r	0019773	cellular_component	proteasome core complex, alpha-subunit complex
r	0042802	molecular_function	identical protein binding
r	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
r	0051603	biological_process	proteolysis involved in protein catabolic process
r	0070062	cellular_component	extracellular exosome
r	0098794	cellular_component	postsynapse
s	0000502	cellular_component	proteasome complex
s	0005515	molecular_function	protein binding
s	0005576	cellular_component	extracellular region
s	0005634	cellular_component	nucleus
s	0005654	cellular_component	nucleoplasm
s	0005737	cellular_component	cytoplasm
s	0005829	cellular_component	cytosol
s	0005839	cellular_component	proteasome core complex
s	0006511	biological_process	ubiquitin-dependent protein catabolic process
s	0019773	cellular_component	proteasome core complex, alpha-subunit complex
s	0034774	cellular_component	secretory granule lumen
s	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
s	0051603	biological_process	proteolysis involved in protein catabolic process
s	0070062	cellular_component	extracellular exosome
s	1904813	cellular_component	ficolin-1-rich granule lumen
T	0000502	cellular_component	proteasome complex
T	0001530	molecular_function	lipopolysaccharide binding
T	0002376	biological_process	immune system process
T	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005654	cellular_component	nucleoplasm
T	0005737	cellular_component	cytoplasm
T	0005813	cellular_component	centrosome
T	0005829	cellular_component	cytosol
T	0005839	cellular_component	proteasome core complex
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
T	0019773	cellular_component	proteasome core complex, alpha-subunit complex
T	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
T	0051603	biological_process	proteolysis involved in protein catabolic process
T	0070062	cellular_component	extracellular exosome
U	0000502	cellular_component	proteasome complex
U	0005515	molecular_function	protein binding
U	0005634	cellular_component	nucleus
U	0005654	cellular_component	nucleoplasm
U	0005737	cellular_component	cytoplasm
U	0005829	cellular_component	cytosol
U	0005839	cellular_component	proteasome core complex
U	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0019773	cellular_component	proteasome core complex, alpha-subunit complex
U	0031625	molecular_function	ubiquitin protein ligase binding
U	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
U	0045202	cellular_component	synapse
U	0051603	biological_process	proteolysis involved in protein catabolic process
U	0052548	biological_process	regulation of endopeptidase activity
U	0070062	cellular_component	extracellular exosome
V	0000502	cellular_component	proteasome complex
V	0002376	biological_process	immune system process
V	0004175	molecular_function	endopeptidase activity
V	0004298	molecular_function	threonine-type endopeptidase activity
V	0005515	molecular_function	protein binding
V	0005634	cellular_component	nucleus
V	0005654	cellular_component	nucleoplasm
V	0005737	cellular_component	cytoplasm
V	0005829	cellular_component	cytosol
V	0005839	cellular_component	proteasome core complex
V	0006508	biological_process	proteolysis
V	0019774	cellular_component	proteasome core complex, beta-subunit complex
V	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
V	0051603	biological_process	proteolysis involved in protein catabolic process
V	0070062	cellular_component	extracellular exosome
V	1990111	cellular_component	spermatoproteasome complex
V	2000116	biological_process	regulation of cysteine-type endopeptidase activity
w	0004298	molecular_function	threonine-type endopeptidase activity
w	0005839	cellular_component	proteasome core complex
w	0051603	biological_process	proteolysis involved in protein catabolic process
X	0000502	cellular_component	proteasome complex
X	0005515	molecular_function	protein binding
X	0005634	cellular_component	nucleus
X	0005654	cellular_component	nucleoplasm
X	0005737	cellular_component	cytoplasm
X	0005829	cellular_component	cytosol
X	0005839	cellular_component	proteasome core complex
X	0019774	cellular_component	proteasome core complex, beta-subunit complex
X	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
X	0051603	biological_process	proteolysis involved in protein catabolic process
X	0070062	cellular_component	extracellular exosome
Y	0000502	cellular_component	proteasome complex
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005654	cellular_component	nucleoplasm
Y	0005737	cellular_component	cytoplasm
Y	0005829	cellular_component	cytosol
Y	0005839	cellular_component	proteasome core complex
Y	0010243	biological_process	response to organonitrogen compound
Y	0010498	biological_process	proteasomal protein catabolic process
Y	0014070	biological_process	response to organic cyclic compound
Y	0016020	cellular_component	membrane
Y	0019774	cellular_component	proteasome core complex, beta-subunit complex
Y	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Y	0051603	biological_process	proteolysis involved in protein catabolic process
Y	0070062	cellular_component	extracellular exosome
Z	0004298	molecular_function	threonine-type endopeptidase activity
Z	0005839	cellular_component	proteasome core complex
Z	0051603	biological_process	proteolysis involved in protein catabolic process

Functional Information from PROSITE/UniProt

site_id	PS00388
Number of Residues	23
Details	PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdlsaStFSPdGRvfQVEYAmkA

Chain	Residue	Details
G	TYR7-ALA29
D	TYR3-ALA25
B	TYR5-ALA27
A	PHE9-ALA31
C	TYR5-ALA27
F	TYR6-ALA28

---

site_id	PS00854
Number of Residues	48
Details	PROTEASOME_BETA_1 Proteasome beta-type subunits signature. IGIqGpdYVLVASDrvaasnivqmkddhd.KmfkmsekilllcvGeaGD

Chain	Residue	Details
K	ILE5-ASP52
L	LEU4-ASP51
H	MET4-ASP51
w	ALA4-ASP51
2	LEU12-ASP59
C	LEU35-ASP82
J	MET12-ASP59
1	LEU13-ASP60

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
G	LYS56
G	LYS205
G	LYS229
U	LYS56
U	LYS205
U	LYS229

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17323924, ECO:0007744|PubMed:23186163

Chain	Residue	Details
G	SER242
U	SER242
B	SER15
P	SER6
P	SER13
P	SER15

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	CARBOHYD: O-linked (GlcNAc) serine; alternate => ECO:0000250

Chain	Residue	Details
F	SER110
T	SER110
Q	LYS127
Q	LYS176

---

site_id	SWS_FT_FI4
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Chain	Residue	Details
F	LYS115
s	SER63
F	LYS208
T	LYS115
T	LYS208

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	CARBOHYD: O-linked (GlcNAc) serine => ECO:0000250

Chain	Residue	Details
E	SER198
s	SER198

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P17220

Chain	Residue	Details
B	TYR120
P	TYR120

---