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6ZXO

Crystal structure of His-tagged human thymidylate synthase (HT-hTS) in complex with FdUMP and Raltitrexed (Tomudex)

Functional Information from GO Data
ChainGOidnamespacecontents
A0000900molecular_functionmRNA regulatory element binding translation repressor activity
A0003729molecular_functionmRNA binding
A0004799molecular_functionthymidylate synthase activity
A0005542molecular_functionfolic acid binding
A0005634cellular_componentnucleus
A0005737cellular_componentcytoplasm
A0005739cellular_componentmitochondrion
A0005743cellular_componentmitochondrial inner membrane
A0005759cellular_componentmitochondrial matrix
A0005829cellular_componentcytosol
A0006206biological_processpyrimidine nucleobase metabolic process
A0006231biological_processdTMP biosynthetic process
A0006235biological_processdTTP biosynthetic process
A0006417biological_processregulation of translation
A0007623biological_processcircadian rhythm
A0008168molecular_functionmethyltransferase activity
A0009165biological_processnucleotide biosynthetic process
A0009410biological_processresponse to xenobiotic stimulus
A0009636biological_processresponse to toxic substance
A0014070biological_processresponse to organic cyclic compound
A0016741molecular_functiontransferase activity, transferring one-carbon groups
A0017148biological_processnegative regulation of translation
A0019860biological_processuracil metabolic process
A0032259biological_processmethylation
A0032570biological_processresponse to progesterone
A0033189biological_processresponse to vitamin A
A0034097biological_processresponse to cytokine
A0035999biological_processtetrahydrofolate interconversion
A0042803molecular_functionprotein homodimerization activity
A0045471biological_processresponse to ethanol
A0046653biological_processtetrahydrofolate metabolic process
A0046683biological_processresponse to organophosphorus
A0048589biological_processdevelopmental growth
A0051216biological_processcartilage development
A0051384biological_processresponse to glucocorticoid
A0051593biological_processresponse to folic acid
A0060574biological_processintestinal epithelial cell maturation
A0071897biological_processDNA biosynthetic process
A0097421biological_processliver regeneration
A1901363molecular_functionheterocyclic compound binding
A1990825molecular_functionsequence-specific mRNA binding
B0000900molecular_functionmRNA regulatory element binding translation repressor activity
B0003729molecular_functionmRNA binding
B0004799molecular_functionthymidylate synthase activity
B0005542molecular_functionfolic acid binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005743cellular_componentmitochondrial inner membrane
B0005759cellular_componentmitochondrial matrix
B0005829cellular_componentcytosol
B0006206biological_processpyrimidine nucleobase metabolic process
B0006231biological_processdTMP biosynthetic process
B0006235biological_processdTTP biosynthetic process
B0006417biological_processregulation of translation
B0007623biological_processcircadian rhythm
B0008168molecular_functionmethyltransferase activity
B0009165biological_processnucleotide biosynthetic process
B0009410biological_processresponse to xenobiotic stimulus
B0009636biological_processresponse to toxic substance
B0014070biological_processresponse to organic cyclic compound
B0016741molecular_functiontransferase activity, transferring one-carbon groups
B0017148biological_processnegative regulation of translation
B0019860biological_processuracil metabolic process
B0032259biological_processmethylation
B0032570biological_processresponse to progesterone
B0033189biological_processresponse to vitamin A
B0034097biological_processresponse to cytokine
B0035999biological_processtetrahydrofolate interconversion
B0042803molecular_functionprotein homodimerization activity
B0045471biological_processresponse to ethanol
B0046653biological_processtetrahydrofolate metabolic process
B0046683biological_processresponse to organophosphorus
B0048589biological_processdevelopmental growth
B0051216biological_processcartilage development
B0051384biological_processresponse to glucocorticoid
B0051593biological_processresponse to folic acid
B0060574biological_processintestinal epithelial cell maturation
B0071897biological_processDNA biosynthetic process
B0097421biological_processliver regeneration
B1901363molecular_functionheterocyclic compound binding
B1990825molecular_functionsequence-specific mRNA binding
C0000900molecular_functionmRNA regulatory element binding translation repressor activity
C0003729molecular_functionmRNA binding
C0004799molecular_functionthymidylate synthase activity
C0005542molecular_functionfolic acid binding
C0005634cellular_componentnucleus
C0005737cellular_componentcytoplasm
C0005739cellular_componentmitochondrion
C0005743cellular_componentmitochondrial inner membrane
C0005759cellular_componentmitochondrial matrix
C0005829cellular_componentcytosol
C0006206biological_processpyrimidine nucleobase metabolic process
C0006231biological_processdTMP biosynthetic process
C0006235biological_processdTTP biosynthetic process
C0006417biological_processregulation of translation
C0007623biological_processcircadian rhythm
C0008168molecular_functionmethyltransferase activity
C0009165biological_processnucleotide biosynthetic process
C0009410biological_processresponse to xenobiotic stimulus
C0009636biological_processresponse to toxic substance
C0014070biological_processresponse to organic cyclic compound
C0016741molecular_functiontransferase activity, transferring one-carbon groups
C0017148biological_processnegative regulation of translation
C0019860biological_processuracil metabolic process
C0032259biological_processmethylation
C0032570biological_processresponse to progesterone
C0033189biological_processresponse to vitamin A
C0034097biological_processresponse to cytokine
C0035999biological_processtetrahydrofolate interconversion
C0042803molecular_functionprotein homodimerization activity
C0045471biological_processresponse to ethanol
C0046653biological_processtetrahydrofolate metabolic process
C0046683biological_processresponse to organophosphorus
C0048589biological_processdevelopmental growth
C0051216biological_processcartilage development
C0051384biological_processresponse to glucocorticoid
C0051593biological_processresponse to folic acid
C0060574biological_processintestinal epithelial cell maturation
C0071897biological_processDNA biosynthetic process
C0097421biological_processliver regeneration
C1901363molecular_functionheterocyclic compound binding
C1990825molecular_functionsequence-specific mRNA binding
D0000900molecular_functionmRNA regulatory element binding translation repressor activity
D0003729molecular_functionmRNA binding
D0004799molecular_functionthymidylate synthase activity
D0005542molecular_functionfolic acid binding
D0005634cellular_componentnucleus
D0005737cellular_componentcytoplasm
D0005739cellular_componentmitochondrion
D0005743cellular_componentmitochondrial inner membrane
D0005759cellular_componentmitochondrial matrix
D0005829cellular_componentcytosol
D0006206biological_processpyrimidine nucleobase metabolic process
D0006231biological_processdTMP biosynthetic process
D0006235biological_processdTTP biosynthetic process
D0006417biological_processregulation of translation
D0007623biological_processcircadian rhythm
D0008168molecular_functionmethyltransferase activity
D0009165biological_processnucleotide biosynthetic process
D0009410biological_processresponse to xenobiotic stimulus
D0009636biological_processresponse to toxic substance
D0014070biological_processresponse to organic cyclic compound
D0016741molecular_functiontransferase activity, transferring one-carbon groups
D0017148biological_processnegative regulation of translation
D0019860biological_processuracil metabolic process
D0032259biological_processmethylation
D0032570biological_processresponse to progesterone
D0033189biological_processresponse to vitamin A
D0034097biological_processresponse to cytokine
D0035999biological_processtetrahydrofolate interconversion
D0042803molecular_functionprotein homodimerization activity
D0045471biological_processresponse to ethanol
D0046653biological_processtetrahydrofolate metabolic process
D0046683biological_processresponse to organophosphorus
D0048589biological_processdevelopmental growth
D0051216biological_processcartilage development
D0051384biological_processresponse to glucocorticoid
D0051593biological_processresponse to folic acid
D0060574biological_processintestinal epithelial cell maturation
D0071897biological_processDNA biosynthetic process
D0097421biological_processliver regeneration
D1901363molecular_functionheterocyclic compound binding
D1990825molecular_functionsequence-specific mRNA binding
E0000900molecular_functionmRNA regulatory element binding translation repressor activity
E0003729molecular_functionmRNA binding
E0004799molecular_functionthymidylate synthase activity
E0005542molecular_functionfolic acid binding
E0005634cellular_componentnucleus
E0005737cellular_componentcytoplasm
E0005739cellular_componentmitochondrion
E0005743cellular_componentmitochondrial inner membrane
E0005759cellular_componentmitochondrial matrix
E0005829cellular_componentcytosol
E0006206biological_processpyrimidine nucleobase metabolic process
E0006231biological_processdTMP biosynthetic process
E0006235biological_processdTTP biosynthetic process
E0006417biological_processregulation of translation
E0007623biological_processcircadian rhythm
E0008168molecular_functionmethyltransferase activity
E0009165biological_processnucleotide biosynthetic process
E0009410biological_processresponse to xenobiotic stimulus
E0009636biological_processresponse to toxic substance
E0014070biological_processresponse to organic cyclic compound
E0016741molecular_functiontransferase activity, transferring one-carbon groups
E0017148biological_processnegative regulation of translation
E0019860biological_processuracil metabolic process
E0032259biological_processmethylation
E0032570biological_processresponse to progesterone
E0033189biological_processresponse to vitamin A
E0034097biological_processresponse to cytokine
E0035999biological_processtetrahydrofolate interconversion
E0042803molecular_functionprotein homodimerization activity
E0045471biological_processresponse to ethanol
E0046653biological_processtetrahydrofolate metabolic process
E0046683biological_processresponse to organophosphorus
E0048589biological_processdevelopmental growth
E0051216biological_processcartilage development
E0051384biological_processresponse to glucocorticoid
E0051593biological_processresponse to folic acid
E0060574biological_processintestinal epithelial cell maturation
E0071897biological_processDNA biosynthetic process
E0097421biological_processliver regeneration
E1901363molecular_functionheterocyclic compound binding
E1990825molecular_functionsequence-specific mRNA binding
F0000900molecular_functionmRNA regulatory element binding translation repressor activity
F0003729molecular_functionmRNA binding
F0004799molecular_functionthymidylate synthase activity
F0005542molecular_functionfolic acid binding
F0005634cellular_componentnucleus
F0005737cellular_componentcytoplasm
F0005739cellular_componentmitochondrion
F0005743cellular_componentmitochondrial inner membrane
F0005759cellular_componentmitochondrial matrix
F0005829cellular_componentcytosol
F0006206biological_processpyrimidine nucleobase metabolic process
F0006231biological_processdTMP biosynthetic process
F0006235biological_processdTTP biosynthetic process
F0006417biological_processregulation of translation
F0007623biological_processcircadian rhythm
F0008168molecular_functionmethyltransferase activity
F0009165biological_processnucleotide biosynthetic process
F0009410biological_processresponse to xenobiotic stimulus
F0009636biological_processresponse to toxic substance
F0014070biological_processresponse to organic cyclic compound
F0016741molecular_functiontransferase activity, transferring one-carbon groups
F0017148biological_processnegative regulation of translation
F0019860biological_processuracil metabolic process
F0032259biological_processmethylation
F0032570biological_processresponse to progesterone
F0033189biological_processresponse to vitamin A
F0034097biological_processresponse to cytokine
F0035999biological_processtetrahydrofolate interconversion
F0042803molecular_functionprotein homodimerization activity
F0045471biological_processresponse to ethanol
F0046653biological_processtetrahydrofolate metabolic process
F0046683biological_processresponse to organophosphorus
F0048589biological_processdevelopmental growth
F0051216biological_processcartilage development
F0051384biological_processresponse to glucocorticoid
F0051593biological_processresponse to folic acid
F0060574biological_processintestinal epithelial cell maturation
F0071897biological_processDNA biosynthetic process
F0097421biological_processliver regeneration
F1901363molecular_functionheterocyclic compound binding
F1990825molecular_functionsequence-specific mRNA binding
Functional Information from PROSITE/UniProt
site_idPS00091
Number of Residues29
DetailsTHYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNprdlplma.....LpPCHalcQFyV
ChainResidueDetails
AARG175-VAL203
FARG175-VAL203

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues1
DetailsACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P0A884
ChainResidueDetails
FCYS195
BCYS195
CCYS195
DCYS195
ECYS195

site_idSWS_FT_FI2
Number of Residues2
DetailsBINDING: in other chain => ECO:0000250|UniProtKB:P0A884
ChainResidueDetails
FARG50
FASN226
BARG50
BASN226
CARG50
CASN226
DARG50
DASN226
EARG50
EASN226

site_idSWS_FT_FI3
Number of Residues1
DetailsBINDING: BINDING => ECO:0000250|UniProtKB:P45352
ChainResidueDetails
CARG175
DARG175
EARG175
FARG175
BARG175

site_idSWS_FT_FI4
Number of Residues3
DetailsBINDING: in other chain => ECO:0000250|UniProtKB:P45352
ChainResidueDetails
BHIS256
CCYS195
CARG215
CHIS256
DCYS195
DARG215
DHIS256
ECYS195
EARG215
EHIS256
BARG215
FCYS195
FARG215
FHIS256
BCYS195

site_idSWS_FT_FI5
Number of Residues2
DetailsBINDING: BINDING => ECO:0000250|UniProtKB:P0A884
ChainResidueDetails
CALA312
DASP218
DALA312
EASP218
EALA312
BASP218
BALA312
CASP218
FASP218
FALA312

site_idSWS_FT_FI6
Number of Residues1
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:23186163
ChainResidueDetails
BSER114
CSER114
DSER114
ESER114
FSER114

site_idSWS_FT_FI7
Number of Residues4
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733
ChainResidueDetails
FLYS287
FLYS292
FLYS308
BLYS287
BLYS292
BLYS308
CLYS287
CLYS292
CLYS308
DLYS287
DLYS292
DLYS308
ELYS287
ELYS292
ELYS308

219869

PDB entries from 2024-05-15

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