6Y7T

Engineered conjugation of lysine-specific molecular tweezers with ExoS derived peptidic inhibitor enhance affinity towards target protein 14-3-3 through ditopic binding

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0001836	biological_process	release of cytochrome c from mitochondria
A	0003334	biological_process	keratinocyte development
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005615	cellular_component	extracellular space
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006469	biological_process	negative regulation of protein kinase activity
A	0006611	biological_process	protein export from nucleus
A	0007165	biological_process	signal transduction
A	0008104	biological_process	protein localization
A	0008426	molecular_function	protein kinase C inhibitor activity
A	0008630	biological_process	intrinsic apoptotic signaling pathway in response to DNA damage
A	0010482	biological_process	regulation of epidermal cell division
A	0010737	biological_process	protein kinase A signaling
A	0010839	biological_process	negative regulation of keratinocyte proliferation
A	0019901	molecular_function	protein kinase binding
A	0030216	biological_process	keratinocyte differentiation
A	0030307	biological_process	positive regulation of cell growth
A	0031424	biological_process	keratinization
A	0042802	molecular_function	identical protein binding
A	0043588	biological_process	skin development
A	0043616	biological_process	keratinocyte proliferation
A	0045296	molecular_function	cadherin binding
A	0045606	biological_process	positive regulation of epidermal cell differentiation
A	0045785	biological_process	positive regulation of cell adhesion
A	0045824	biological_process	negative regulation of innate immune response
A	0046827	biological_process	positive regulation of protein export from nucleus
A	0050815	molecular_function	phosphoserine residue binding
A	0051219	molecular_function	phosphoprotein binding
A	0051726	biological_process	regulation of cell cycle
A	0061436	biological_process	establishment of skin barrier
A	0070062	cellular_component	extracellular exosome
A	0072089	biological_process	stem cell proliferation
A	0140311	molecular_function	protein sequestering activity
A	1903077	biological_process	negative regulation of protein localization to plasma membrane
A	1903829	biological_process	positive regulation of protein localization
A	2000647	biological_process	negative regulation of stem cell proliferation
F	0000122	biological_process	negative regulation of transcription by RNA polymerase II
F	0001836	biological_process	release of cytochrome c from mitochondria
F	0003334	biological_process	keratinocyte development
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005615	cellular_component	extracellular space
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006469	biological_process	negative regulation of protein kinase activity
F	0006611	biological_process	protein export from nucleus
F	0007165	biological_process	signal transduction
F	0008104	biological_process	protein localization
F	0008426	molecular_function	protein kinase C inhibitor activity
F	0008630	biological_process	intrinsic apoptotic signaling pathway in response to DNA damage
F	0010482	biological_process	regulation of epidermal cell division
F	0010737	biological_process	protein kinase A signaling
F	0010839	biological_process	negative regulation of keratinocyte proliferation
F	0019901	molecular_function	protein kinase binding
F	0030216	biological_process	keratinocyte differentiation
F	0030307	biological_process	positive regulation of cell growth
F	0031424	biological_process	keratinization
F	0042802	molecular_function	identical protein binding
F	0043588	biological_process	skin development
F	0043616	biological_process	keratinocyte proliferation
F	0045296	molecular_function	cadherin binding
F	0045606	biological_process	positive regulation of epidermal cell differentiation
F	0045785	biological_process	positive regulation of cell adhesion
F	0045824	biological_process	negative regulation of innate immune response
F	0046827	biological_process	positive regulation of protein export from nucleus
F	0050815	molecular_function	phosphoserine residue binding
F	0051219	molecular_function	phosphoprotein binding
F	0051726	biological_process	regulation of cell cycle
F	0061436	biological_process	establishment of skin barrier
F	0070062	cellular_component	extracellular exosome
F	0072089	biological_process	stem cell proliferation
F	0140311	molecular_function	protein sequestering activity
F	1903077	biological_process	negative regulation of protein localization to plasma membrane
F	1903829	biological_process	positive regulation of protein localization
F	2000647	biological_process	negative regulation of stem cell proliferation
K	0000122	biological_process	negative regulation of transcription by RNA polymerase II
K	0001836	biological_process	release of cytochrome c from mitochondria
K	0003334	biological_process	keratinocyte development
K	0005515	molecular_function	protein binding
K	0005576	cellular_component	extracellular region
K	0005615	cellular_component	extracellular space
K	0005634	cellular_component	nucleus
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0006469	biological_process	negative regulation of protein kinase activity
K	0006611	biological_process	protein export from nucleus
K	0007165	biological_process	signal transduction
K	0008104	biological_process	protein localization
K	0008426	molecular_function	protein kinase C inhibitor activity
K	0008630	biological_process	intrinsic apoptotic signaling pathway in response to DNA damage
K	0010482	biological_process	regulation of epidermal cell division
K	0010737	biological_process	protein kinase A signaling
K	0010839	biological_process	negative regulation of keratinocyte proliferation
K	0019901	molecular_function	protein kinase binding
K	0030216	biological_process	keratinocyte differentiation
K	0030307	biological_process	positive regulation of cell growth
K	0031424	biological_process	keratinization
K	0042802	molecular_function	identical protein binding
K	0043588	biological_process	skin development
K	0043616	biological_process	keratinocyte proliferation
K	0045296	molecular_function	cadherin binding
K	0045606	biological_process	positive regulation of epidermal cell differentiation
K	0045785	biological_process	positive regulation of cell adhesion
K	0045824	biological_process	negative regulation of innate immune response
K	0046827	biological_process	positive regulation of protein export from nucleus
K	0050815	molecular_function	phosphoserine residue binding
K	0051219	molecular_function	phosphoprotein binding
K	0051726	biological_process	regulation of cell cycle
K	0061436	biological_process	establishment of skin barrier
K	0070062	cellular_component	extracellular exosome
K	0072089	biological_process	stem cell proliferation
K	0140311	molecular_function	protein sequestering activity
K	1903077	biological_process	negative regulation of protein localization to plasma membrane
K	1903829	biological_process	positive regulation of protein localization
K	2000647	biological_process	negative regulation of stem cell proliferation
P	0000122	biological_process	negative regulation of transcription by RNA polymerase II
P	0001836	biological_process	release of cytochrome c from mitochondria
P	0003334	biological_process	keratinocyte development
P	0005515	molecular_function	protein binding
P	0005576	cellular_component	extracellular region
P	0005615	cellular_component	extracellular space
P	0005634	cellular_component	nucleus
P	0005737	cellular_component	cytoplasm
P	0005829	cellular_component	cytosol
P	0006469	biological_process	negative regulation of protein kinase activity
P	0006611	biological_process	protein export from nucleus
P	0007165	biological_process	signal transduction
P	0008104	biological_process	protein localization
P	0008426	molecular_function	protein kinase C inhibitor activity
P	0008630	biological_process	intrinsic apoptotic signaling pathway in response to DNA damage
P	0010482	biological_process	regulation of epidermal cell division
P	0010737	biological_process	protein kinase A signaling
P	0010839	biological_process	negative regulation of keratinocyte proliferation
P	0019901	molecular_function	protein kinase binding
P	0030216	biological_process	keratinocyte differentiation
P	0030307	biological_process	positive regulation of cell growth
P	0031424	biological_process	keratinization
P	0042802	molecular_function	identical protein binding
P	0043588	biological_process	skin development
P	0043616	biological_process	keratinocyte proliferation
P	0045296	molecular_function	cadherin binding
P	0045606	biological_process	positive regulation of epidermal cell differentiation
P	0045785	biological_process	positive regulation of cell adhesion
P	0045824	biological_process	negative regulation of innate immune response
P	0046827	biological_process	positive regulation of protein export from nucleus
P	0050815	molecular_function	phosphoserine residue binding
P	0051219	molecular_function	phosphoprotein binding
P	0051726	biological_process	regulation of cell cycle
P	0061436	biological_process	establishment of skin barrier
P	0070062	cellular_component	extracellular exosome
P	0072089	biological_process	stem cell proliferation
P	0140311	molecular_function	protein sequestering activity
P	1903077	biological_process	negative regulation of protein localization to plasma membrane
P	1903829	biological_process	positive regulation of protein localization
P	2000647	biological_process	negative regulation of stem cell proliferation

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	binding site for residue NA A 301

Chain	Residue
A	HOH439
K	LYS9

---

site_id	AC2
Number of Residues	4
Details	binding site for residue GOL A 302

Chain	Residue
A	GLN93
A	ASP97
A	LEU131
A	ASP139

---

site_id	AC3
Number of Residues	9
Details	binding site for residue TWZ D 501

Chain	Residue
A	ARG224
D	GLN420
D	HOH602
F	TYR213
F	LYS214
F	HOH447
A	MET202
A	MET220
A	GLN221

---

site_id	AC4
Number of Residues	2
Details	binding site for residue NA F 301

Chain	Residue
F	LYS9
P	HOH454

---

site_id	AC5
Number of Residues	1
Details	binding site for residue NA K 301

Chain	Residue
A	LYS9

---

site_id	AC6
Number of Residues	2
Details	binding site for residue NA P 301

Chain	Residue
F	HOH448
P	LYS9

---

site_id	AC7
Number of Residues	4
Details	binding site for residue NA P 302

Chain	Residue
P	ARG3
P	ALA29
P	VAL30
P	GLU34

---

site_id	AC8
Number of Residues	3
Details	binding site for residue GOL P 303

Chain	Residue
P	GLN93
P	ASP97
P	ASP139

---

site_id	AC9
Number of Residues	9
Details	binding site for Di-peptide TWZ E 501 and GLN E 420

Chain	Residue
A	TYR213
A	LYS214
A	HOH442
E	GLY421
E	LEU422
F	MET202
F	MET220
F	GLN221
F	ARG224

---

Functional Information from PROSITE/UniProt

site_id	PS00796
Number of Residues	11
Details	1433_1 14-3-3 proteins signature 1. RNLLSVAYKNV

Chain	Residue	Details
A	ARG41-VAL51

---

site_id	PS00797
Number of Residues	20
Details	1433_2 14-3-3 proteins signature 2. YKDSTLIMQLLRDNLTLWTA

Chain	Residue	Details
A	TYR213-ALA232

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	SITE: Interaction with phosphoserine on interacting protein

Chain	Residue	Details
F	ARG56
F	ARG129
K	ARG56
K	ARG129
P	ARG56
P	ARG129
A	ARG56
A	ARG129

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231

Chain	Residue	Details
P	SER5
A	SER5
F	SER5
K	SER5

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER74
F	SER74
K	SER74
P	SER74

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:16964243, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:20068231

Chain	Residue	Details
A	SER248
F	SER248
K	SER248
P	SER248

---