6XZ1

Conjugate of the HECT domain of HUWE1 with ubiquitin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004842	molecular_function	ubiquitin-protein transferase activity
B	0004842	molecular_function	ubiquitin-protein transferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	3
Details	binding site for residue PO4 A 4401

Chain	Residue
A	ARG4142
A	TYR4143
A	HIS4208

---

site_id	AC2
Number of Residues	4
Details	binding site for residue PO4 A 4402

Chain	Residue
A	ARG4283
A	HOH4518
B	LYS4263
B	ARG4286

---

site_id	AC3
Number of Residues	5
Details	binding site for residue AYE C 101

Chain	Residue
A	CYS4341
A	ALA4374
C	GLY75
A	GLN4056
A	THR4340

---

site_id	AC4
Number of Residues	3
Details	binding site for residue PO4 B 4401

Chain	Residue
B	HIS4035
B	SER4105
B	HOH4517

---

site_id	AC5
Number of Residues	3
Details	binding site for residue PO4 B 4402

Chain	Residue
B	ARG4142
B	TYR4143
B	HIS4208

---

site_id	AC6
Number of Residues	4
Details	binding site for residue PO4 D 102

Chain	Residue
B	ARG4334
B	LEU4373
D	ASP39
D	GLN40

---

site_id	AC7
Number of Residues	10
Details	binding site for Di-peptide AYE D 101 and GLY D 75

Chain	Residue
B	GLN4056
B	LEU4061
B	ALA4338
B	HIS4339
B	THR4340
B	CYS4341
B	PHE4371
B	LEU4373
B	ALA4374
D	ARG74

---

site_id	AC8
Number of Residues	13
Details	binding site for Di-peptide AYE D 101 and CYS B 4341

Chain	Residue
B	GLN4056
B	ALA4058
B	GLY4060
B	LEU4061
B	ARG4063
B	GLY4316
B	MET4317
B	HIS4339
B	THR4340
B	PHE4342
B	ASN4343
B	ALA4374
D	GLY75

---

Functional Information from PROSITE/UniProt

site_id	PS00299
Number of Residues	26
Details	UBIQUITIN_1 Ubiquitin domain signature. KakIqDkegIPpdqQrLIFaGkqleD

Chain	Residue	Details
C	LYS27-ASP52

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	SITE: Interacts with activating enzyme

Chain	Residue	Details
C	ARG54
C	ARG72
D	ARG54
D	ARG72

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	SITE: Essential for function

Chain	Residue	Details
C	HIS68
D	HIS68

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine; by PINK1 => ECO:0000269|PubMed:24660806, ECO:0000269|PubMed:24751536, ECO:0000269|PubMed:24784582, ECO:0000269|PubMed:25527291

Chain	Residue	Details
C	SER65
D	SER65

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: (Microbial infection) ADP-ribosylthreonine => ECO:0000269|PubMed:32330457

Chain	Residue	Details
C	THR66
D	THR66

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603

Chain	Residue	Details
C	LYS6
D	LYS6

---

site_id	SWS_FT_FI6
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:18719106

Chain	Residue	Details
C	LYS63
D	LYS63

---

site_id	SWS_FT_FI7
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16443603, ECO:0000269|PubMed:16543144

Chain	Residue	Details
C	LYS11
C	LYS48
D	LYS11
D	LYS48

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000305|PubMed:15466860

Chain	Residue	Details
C	LYS27
D	LYS27

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:16543144, ECO:0000269|PubMed:25752573, ECO:0000269|PubMed:25752577, ECO:0000269|PubMed:34239127

Chain	Residue	Details
C	LYS29
D	LYS29

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:25752577

Chain	Residue	Details
C	LYS33
D	LYS33

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