Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6VZS

Engineered TTLL6 mutant bound to gamma-elongation analog

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0036211	biological_process	protein modification process
B	0036211	biological_process	protein modification process
C	0036211	biological_process	protein modification process
D	0036211	biological_process	protein modification process

Functional Information from PDB Data

site_id	AC1
Number of Residues	25
Details	binding site for residue ADP A 601

Chain	Residue
A	LYS125
A	TYR204
A	ILE205
A	LYS215
A	ASP217
A	LEU262
A	THR263
A	ASN264
A	ASP346
A	LEU348
A	LEU358
A	ILE172
A	GLU359
A	S3A602
A	MG613
A	MG614
A	HOH711
A	HOH724
A	LYS174
A	ALA179
A	ARG180
A	GLY181
A	ILE184
A	GLN202
A	LEU203

site_id	AC2
Number of Residues	21
Details	binding site for residue S3A A 602

Chain	Residue
A	ARG180
A	ARG219
A	LEU239
A	ARG241
A	ASN264
A	TYR265
A	SER266
A	LYS283
A	ASP346
A	GLU359
A	ASN361
A	ILE362
A	SER363
A	SER365
A	ASP373
A	LYS377
A	ADP601
A	MG613
A	MG614
A	HOH724
C	LYS194

site_id	AC3
Number of Residues	3
Details	binding site for residue GOL A 603

Chain	Residue
A	CYS259
A	SER271
A	HOH731

site_id	AC4
Number of Residues	2
Details	binding site for residue GOL A 604

Chain	Residue
A	ARG148
A	HOH738

site_id	AC5
Number of Residues	5
Details	binding site for residue GOL A 605

Chain	Residue
A	ARG68
A	TYR69
A	ASP70
A	SER367
A	THR368

site_id	AC6
Number of Residues	6
Details	binding site for residue GOL A 606

Chain	Residue
A	SER266
A	LYS269
A	HIS270
A	HOH735
C	GLU192
C	GOL1201

site_id	AC7
Number of Residues	5
Details	binding site for residue GOL A 607

Chain	Residue
A	ASN290
A	GLU301
A	ARG305
A	ARG445
A	HOH717

site_id	AC8
Number of Residues	5
Details	binding site for residue GOL A 608

Chain	Residue
A	PRO175
A	ASP198
A	MET199
C	PRO175
C	MET199

site_id	AC9
Number of Residues	9
Details	binding site for residue GOL A 609

Chain	Residue
A	PHE214
A	PHE242
A	ALA243
A	ASN273
A	VAL275
A	SER280
A	GLY281
A	SER282
A	ARG284

site_id	AD1
Number of Residues	1
Details	binding site for residue GOL A 610

Chain	Residue
A	ARG351

site_id	AD2
Number of Residues	5
Details	binding site for residue GOL A 611

Chain	Residue
A	ASP309
A	LYS313
A	LYS455
A	TYR456
A	PHE459

site_id	AD3
Number of Residues	2
Details	binding site for residue GOL A 612

Chain	Residue
A	GLU237
A	SER287

site_id	AD4
Number of Residues	6
Details	binding site for residue MG A 613

Chain	Residue
A	ASP346
A	GLU359
A	ADP601
A	S3A602
A	MG614
A	HOH724

site_id	AD5
Number of Residues	6
Details	binding site for residue MG A 614

Chain	Residue
A	MG613
A	HOH740
A	GLU359
A	ASN361
A	ADP601
A	S3A602

site_id	AD6
Number of Residues	24
Details	binding site for residue ADP B 601

Chain	Residue
B	LYS125
B	ILE172
B	LYS174
B	ALA179
B	ARG180
B	GLY181
B	ILE184
B	GLN202
B	LEU203
B	TYR204
B	ILE205
B	LYS215
B	ASP217
B	LEU262
B	THR263
B	ASN264
B	ASP346
B	LEU348
B	LEU358
B	GLU359
B	S3A602
B	MG607
B	MG608
B	HOH707

site_id	AD7
Number of Residues	22
Details	binding site for residue S3A B 602

Chain	Residue
B	TYR69
B	ALA179
B	ARG180
B	ARG219
B	LEU239
B	ARG241
B	ASN264
B	TYR265
B	SER266
B	LYS283
B	ASP346
B	GLU359
B	ASN361
B	SER363
B	SER365
B	ASP373
B	LYS377
B	ADP601
B	MG607
B	MG608
B	HOH707
B	HOH716

site_id	AD8
Number of Residues	1
Details	binding site for residue GOL B 603

Chain	Residue
B	ARG351

site_id	AD9
Number of Residues	3
Details	binding site for residue GOL B 604

Chain	Residue
B	ILE210
B	ASP212
B	GLY213

site_id	AE1
Number of Residues	1
Details	binding site for residue GOL B 605

Chain	Residue
B	ASN236

site_id	AE2
Number of Residues	4
Details	binding site for residue GOL B 606

Chain	Residue
B	PRO147
B	ARG148
B	LEU203
B	TRP356

site_id	AE3
Number of Residues	6
Details	binding site for residue MG B 607

Chain	Residue
B	GLY178
B	ALA179
B	GLU359
B	ASN361
B	ADP601
B	S3A602

site_id	AE4
Number of Residues	5
Details	binding site for residue MG B 608

Chain	Residue
B	ASP346
B	GLU359
B	ADP601
B	S3A602
B	HOH707

site_id	AE5
Number of Residues	6
Details	binding site for residue GOL C 1201

Chain	Residue
A	ARG180
A	ARG182
A	GOL606
C	THR187
C	SER189
C	GLU192

site_id	AE6
Number of Residues	25
Details	binding site for residue ADP C 1202

Chain	Residue
C	LYS125
C	ILE172
C	LYS174
C	ALA179
C	ARG180
C	GLY181
C	ILE184
C	GLN202
C	LEU203
C	TYR204
C	ILE205
C	LYS215
C	ASP217
C	LEU262
C	THR263
C	ASN264
C	ASP346
C	LEU348
C	LEU358
C	GLU359
C	S3A1203
C	MG1221
C	MG1222
C	HOH1304
C	HOH1306

site_id	AE7
Number of Residues	27
Details	binding site for residue S3A C 1203

Chain	Residue
A	LYS194
A	HOH710
C	TYR69
C	ALA179
C	ARG180
C	ARG219
C	LEU239
C	ARG241
C	ASN264
C	TYR265
C	SER266
C	LYS283
C	ASP346
C	GLU359
C	ASN361
C	SER363
C	PRO364
C	SER365
C	ASP373
C	LYS377
C	ADP1202
C	GOL1204
C	MG1221
C	MG1222
C	HOH1301
C	HOH1304
C	HOH1324

site_id	AE8
Number of Residues	9
Details	binding site for residue GOL C 1204

Chain	Residue
A	LYS194
C	CYS123
C	ARG124
C	SER177
C	GLY178
C	VAL360
C	ILE362
C	S3A1203
C	HOH1302

site_id	AE9
Number of Residues	1
Details	binding site for residue GOL C 1205

Chain	Residue
C	TYR247

site_id	AF1
Number of Residues	8
Details	binding site for residue GOL C 1206

Chain	Residue
C	PHE214
C	ALA243
C	THR244
C	ASN273
C	VAL275
C	SER280
C	GLY281
C	SER282

site_id	AF2
Number of Residues	6
Details	binding site for residue GOL C 1207

Chain	Residue
C	ARG68
C	TYR69
C	ASP70
C	PHE366
C	SER367
C	THR368

site_id	AF3
Number of Residues	3
Details	binding site for residue GOL C 1208

Chain	Residue
C	GLN302
C	ILE303
C	LEU353

site_id	AF4
Number of Residues	5
Details	binding site for residue GOL C 1209

Chain	Residue
C	ASP350
C	LYS352
C	LYS354
C	TRP356
C	GOL1212

site_id	AF5
Number of Residues	2
Details	binding site for residue GOL C 1210

Chain	Residue
C	ARG351
C	GOL1215

site_id	AF6
Number of Residues	4
Details	binding site for residue GOL C 1212

Chain	Residue
C	LEU203
C	TYR204
C	GOL1209
C	GOL1216

site_id	AF7
Number of Residues	2
Details	binding site for residue GOL C 1213

Chain	Residue
A	PRO195
C	TYR97

site_id	AF8
Number of Residues	2
Details	binding site for residue GOL C 1214

Chain	Residue
C	ARG351
C	GOL1215

site_id	AF9
Number of Residues	7
Details	binding site for residue GOL C 1215

Chain	Residue
C	PRO208
C	PHE209
C	ILE210
C	ARG351
C	GOL1210
C	GOL1214
C	GOL1218

site_id	AG1
Number of Residues	5
Details	binding site for residue GOL C 1216

Chain	Residue
C	HIS144
C	PHE146
C	PRO147
C	ARG148
C	GOL1212

site_id	AG2
Number of Residues	4
Details	binding site for residue GOL C 1217

Chain	Residue
C	ARG305
C	GLY306
C	ASP309
C	LYS455

site_id	AG3
Number of Residues	3
Details	binding site for residue GOL C 1218

Chain	Residue
C	ILE210
C	ASP212
C	GOL1215

site_id	AG4
Number of Residues	4
Details	binding site for residue GOL C 1219

Chain	Residue
C	ALA278
C	LYS285
C	LEU372
C	GLU375

site_id	AG5
Number of Residues	2
Details	binding site for residue GOL C 1220

Chain	Residue
C	GLN78
C	ASP378

site_id	AG6
Number of Residues	6
Details	binding site for residue MG C 1221

Chain	Residue
C	ASP346
C	GLU359
C	ADP1202
C	S3A1203
C	MG1222
C	HOH1304

site_id	AG7
Number of Residues	6
Details	binding site for residue MG C 1222

Chain	Residue
C	GLU359
C	ASN361
C	ADP1202
C	S3A1203
C	MG1221
C	HOH1301

site_id	AG8
Number of Residues	23
Details	binding site for residue ADP D 601

Chain	Residue
D	LYS125
D	ILE172
D	LYS174
D	ALA179
D	ARG180
D	GLY181
D	ILE184
D	GLN202
D	LEU203
D	TYR204
D	ILE205
D	LYS215
D	ASP217
D	LEU262
D	THR263
D	ASN264
D	ASP346
D	LEU348
D	LEU358
D	GLU359
D	S3A602
D	MG604
D	MG605

site_id	AG9
Number of Residues	21
Details	binding site for residue S3A D 602

Chain	Residue
B	LYS194
D	TYR69
D	ALA179
D	ARG180
D	ARG219
D	LEU239
D	ARG241
D	ASN264
D	TYR265
D	SER266
D	LYS283
D	ASP346
D	GLU359
D	ASN361
D	SER363
D	SER365
D	LYS377
D	ADP601
D	MG604
D	MG605
D	HOH707

site_id	AH1
Number of Residues	2
Details	binding site for residue GOL D 603

Chain	Residue
D	ASP212
D	TYR292

site_id	AH2
Number of Residues	5
Details	binding site for residue MG D 604

Chain	Residue
D	ASN264
D	ASP346
D	GLU359
D	ADP601
D	S3A602

site_id	AH3
Number of Residues	4
Details	binding site for residue MG D 605

Chain	Residue
D	GLU359
D	ASN361
D	ADP601
D	S3A602

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:32747782, ECO:0007744\|PDB:6VZT

Chain	Residue	Details
A	LYS174
C	GLN202
C	LYS215
C	THR263
D	LYS174
D	GLN202
D	LYS215
D	THR263
A	GLN202
A	LYS215
A	THR263
B	LYS174
B	GLN202
B	LYS215
B	THR263
C	LYS174

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000305\|PubMed:32747782

Chain	Residue	Details
A	ARG180
A	ILE362
B	ARG180
B	ILE362
C	ARG180
C	ILE362
D	ARG180
D	ILE362

site_id	SWS_FT_FI3
Number of Residues	20
Details	BINDING: BINDING => ECO:0000305\|PubMed:32747782, ECO:0007744\|PDB:6VZU, ECO:0007744\|PDB:6VZW

Chain	Residue	Details
A	ARG241
B	LYS377
C	ARG241
C	TYR265
C	SER266
C	LYS283
C	LYS377
D	ARG241
D	TYR265
D	SER266
D	LYS283
A	TYR265
D	LYS377
A	SER266
A	LYS283
A	LYS377
B	ARG241
B	TYR265
B	SER266
B	LYS283

site_id	SWS_FT_FI4
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:32747782, ECO:0007744\|PDB:6VZU

Chain	Residue	Details
A	ASP346
D	ASP346
D	GLU359
D	ASN361
A	GLU359
A	ASN361
B	ASP346
B	GLU359
B	ASN361
C	ASP346
C	GLU359
C	ASN361

site_id	SWS_FT_FI5
Number of Residues	4
Details	SITE: Essential for specifying alpha-elongation versus initiation step of the polyglutamylase activity => ECO:0000269\|PubMed:32747782

Chain	Residue	Details
A	ARG180
B	ARG180
C	ARG180
D	ARG180

site_id	SWS_FT_FI6
Number of Residues	4
Details	SITE: Important for specifying alpha-elongation versus initiation step of the polyglutamylase activity => ECO:0000269\|PubMed:32747782

Chain	Residue	Details
A	ILE362
B	ILE362
C	ILE362
D	ILE362

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)