6VZS
Engineered TTLL6 mutant bound to gamma-elongation analog
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-29 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9774 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.738, 108.648, 170.888 |
Unit cell angles | 90.00, 90.02, 90.00 |
Refinement procedure
Resolution | 45.840 - 2.660 |
R-factor | 0.2203 |
Rwork | 0.218 |
R-free | 0.25550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ylr |
RMSD bond length | 0.002 |
RMSD bond angle | 0.490 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.840 | 2.755 |
High resolution limit [Å] | 2.660 | 2.660 |
Rmerge | 0.046 | 0.328 |
Rmeas | 0.065 | 0.464 |
Rpim | 0.045 | 0.328 |
Number of reflections | 155180 | 15165 |
<I/σ(I)> | 8.69 | 2.03 |
Completeness [%] | 99.7 | 98.85 |
Redundancy | 2 | 2 |
CC(1/2) | 0.998 | 0.872 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 290 | 100 mM Sodium Citrate, pH 6.2, 200 mM MgCl2, 12 % Peg 20000 |