Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6VZR

Engineered TTLL6 bound to the initiation analog

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0036211	biological_process	protein modification process
B	0036211	biological_process	protein modification process
C	0036211	biological_process	protein modification process
D	0036211	biological_process	protein modification process

Functional Information from PDB Data

site_id	AC1
Number of Residues	30
Details	binding site for residue 2TI A 601

Chain	Residue
A	ARG68
A	SER266
A	LYS283
A	ASP346
A	GLU359
A	VAL360
A	ASN361
A	ILE362
A	SER363
A	PRO364
A	SER365
A	TYR69
A	ASP373
A	LYS377
A	ADP602
A	GOL605
A	MG610
A	MG611
A	HOH710
A	HOH716
A	HOH730
A	HOH734
A	ALA179
C	LYS194
A	ARG180
A	ARG219
A	LEU239
A	ARG241
A	ASN264
A	TYR265

site_id	AC2
Number of Residues	26
Details	binding site for residue ADP A 602

Chain	Residue
A	LYS125
A	ILE172
A	LYS174
A	ALA179
A	ARG180
A	GLY181
A	ILE184
A	GLN202
A	LEU203
A	TYR204
A	ILE205
A	LYS215
A	ASP217
A	LEU262
A	THR263
A	ASN264
A	ASP346
A	LEU348
A	LEU358
A	GLU359
A	2TI601
A	MG610
A	MG611
A	HOH730
A	HOH734
A	HOH761

site_id	AC3
Number of Residues	3
Details	binding site for residue GOL A 603

Chain	Residue
A	ASP142
A	ASP309
A	LYS313

site_id	AC4
Number of Residues	5
Details	binding site for residue GOL A 604

Chain	Residue
A	ARG68
A	TYR69
A	ASP70
A	SER367
A	THR368

site_id	AC5
Number of Residues	9
Details	binding site for residue GOL A 605

Chain	Residue
A	ARG180
A	TYR265
A	LYS283
A	SER365
A	SER367
A	ASP369
A	ASP373
A	LYS377
A	2TI601

site_id	AC6
Number of Residues	6
Details	binding site for residue GOL A 606

Chain	Residue
A	TYR265
A	PHE274
A	GLN276
A	LYS283
A	SER370
A	ASP373

site_id	AC7
Number of Residues	6
Details	binding site for residue GOL A 607

Chain	Residue
A	TYR235
A	GLU237
A	LYS285
A	GLU375
A	VAL376
A	SER379

site_id	AC8
Number of Residues	2
Details	binding site for residue GOL A 608

Chain	Residue
A	LEU203
A	HOH769

site_id	AC9
Number of Residues	5
Details	binding site for residue GOL A 609

Chain	Residue
A	PRO229
A	LEU230
A	HIS319
A	TYR448
A	PHE460

site_id	AD1
Number of Residues	6
Details	binding site for residue MG A 610

Chain	Residue
A	GLU359
A	ASN361
A	2TI601
A	ADP602
A	MG611
A	HOH734

site_id	AD2
Number of Residues	6
Details	binding site for residue MG A 611

Chain	Residue
A	GLU359
A	2TI601
A	ADP602
A	MG610
A	HOH730
A	ASP346

site_id	AD3
Number of Residues	5
Details	binding site for residue GOL A 612

Chain	Residue
A	ASP299
A	GLU301
A	GLN302
A	ARG305
A	HOH767

site_id	AD4
Number of Residues	25
Details	binding site for residue 2TI B 601

Chain	Residue
B	ARG68
B	TYR69
B	GLY178
B	ALA179
B	ARG180
B	ARG219
B	LEU239
B	ARG241
B	ASN264
B	TYR265
B	SER266
B	LYS283
B	ASP346
B	GLU359
B	ASN361
B	ILE362
B	SER363
B	SER365
B	ASP373
B	LYS377
B	ADP602
B	MG606
B	MG607
B	HOH712
D	LYS194

site_id	AD5
Number of Residues	23
Details	binding site for residue ADP B 602

Chain	Residue
B	LYS125
B	ILE172
B	LYS174
B	ALA179
B	ARG180
B	GLY181
B	ILE184
B	GLN202
B	LEU203
B	TYR204
B	ILE205
B	LYS215
B	ASP217
B	LEU262
B	THR263
B	ASN264
B	ASP346
B	LEU348
B	LEU358
B	GLU359
B	2TI601
B	MG606
B	MG607

site_id	AD6
Number of Residues	4
Details	binding site for residue GOL B 603

Chain	Residue
B	ARG59
B	GLN77
B	GLY80
B	LEU81

site_id	AD7
Number of Residues	5
Details	binding site for residue GOL B 604

Chain	Residue
B	GLU301
B	ARG305
B	ARG445
B	GOL605
B	HOH734

site_id	AD8
Number of Residues	6
Details	binding site for residue GOL B 605

Chain	Residue
B	SER287
B	ASN290
B	SER291
B	LYS294
B	GLU301
B	GOL604

site_id	AD9
Number of Residues	6
Details	binding site for residue MG B 606

Chain	Residue
B	ARG241
B	ASN264
B	ASP346
B	GLU359
B	2TI601
B	ADP602

site_id	AE1
Number of Residues	4
Details	binding site for residue MG B 607

Chain	Residue
B	GLU359
B	ASN361
B	2TI601
B	ADP602

site_id	AE2
Number of Residues	2
Details	binding site for residue GOL B 609

Chain	Residue
B	ARG148
B	TYR162

site_id	AE3
Number of Residues	3
Details	binding site for residue GOL B 610

Chain	Residue
B	LEU203
B	TYR204
B	SER206

site_id	AE4
Number of Residues	5
Details	binding site for residue GOL B 611

Chain	Residue
B	TYR265
B	PHE274
B	GLN276
B	SER370
B	HOH704

site_id	AE5
Number of Residues	29
Details	binding site for residue 2TI C 601

Chain	Residue
A	LYS194
C	ARG68
C	TYR69
C	ALA179
C	ARG180
C	ARG219
C	LEU239
C	ARG241
C	ASN264
C	TYR265
C	SER266
C	LYS283
C	ASP346
C	GLU359
C	VAL360
C	ASN361
C	ILE362
C	SER363
C	PRO364
C	SER365
C	ASP373
C	LYS377
C	ADP602
C	MG608
C	MG609
C	HOH719
C	HOH725
C	HOH733
C	HOH765

site_id	AE6
Number of Residues	25
Details	binding site for residue ADP C 602

Chain	Residue
C	LYS125
C	ILE172
C	LYS174
C	ALA179
C	ARG180
C	GLY181
C	ILE184
C	GLN202
C	LEU203
C	TYR204
C	ILE205
C	LYS215
C	ASP217
C	LEU262
C	THR263
C	ASN264
C	ASP346
C	LEU348
C	LEU358
C	GLU359
C	2TI601
C	MG608
C	MG609
C	HOH733
C	HOH768

site_id	AE7
Number of Residues	3
Details	binding site for residue GOL C 603

Chain	Residue
C	ARG59
C	GLN77
C	LEU81

site_id	AE8
Number of Residues	3
Details	binding site for residue GOL C 604

Chain	Residue
C	GLU301
C	ARG305
C	ARG445

site_id	AE9
Number of Residues	4
Details	binding site for residue GOL C 605

Chain	Residue
C	SER291
C	TYR292
C	THR295
C	HIS296

site_id	AF1
Number of Residues	4
Details	binding site for residue GOL C 606

Chain	Residue
C	GLN78
C	ASP378
C	SER379
C	HOH723

site_id	AF2
Number of Residues	7
Details	binding site for residue GOL C 607

Chain	Residue
C	SER206
C	LYS207
C	PRO208
C	ILE210
C	TYR247
C	HIS249
C	ARG351

site_id	AF3
Number of Residues	6
Details	binding site for residue MG C 608

Chain	Residue
C	ASP346
C	GLU359
C	2TI601
C	ADP602
C	MG609
C	HOH733

site_id	AF4
Number of Residues	6
Details	binding site for residue MG C 609

Chain	Residue
C	GLU359
C	ASN361
C	2TI601
C	ADP602
C	MG608
C	HOH725

site_id	AF5
Number of Residues	5
Details	binding site for residue GOL C 610

Chain	Residue
C	ARG68
C	TYR69
C	ASP70
C	THR368
C	HOH713

site_id	AF6
Number of Residues	2
Details	binding site for residue GOL C 611

Chain	Residue
C	LEU203
C	HOH763

site_id	AF7
Number of Residues	2
Details	binding site for residue GOL C 612

Chain	Residue
C	ASP212
C	TYR292

site_id	AF8
Number of Residues	4
Details	binding site for residue GOL C 613

Chain	Residue
C	GLU437
C	CYS441
C	ARG445
C	LEU446

site_id	AF9
Number of Residues	28
Details	binding site for residue 2TI D 601

Chain	Residue
B	LYS194
D	ARG68
D	TYR69
D	CYS123
D	ALA179
D	ARG180
D	ARG219
D	LEU239
D	ARG241
D	ASN264
D	TYR265
D	SER266
D	LYS283
D	ASP346
D	GLU359
D	VAL360
D	ASN361
D	ILE362
D	SER363
D	PRO364
D	SER365
D	ASP373
D	LYS377
D	ADP602
D	MG604
D	MG605
D	HOH710
D	HOH717

site_id	AG1
Number of Residues	25
Details	binding site for residue ADP D 602

Chain	Residue
D	LYS125
D	ILE172
D	LYS174
D	ALA179
D	ARG180
D	GLY181
D	ILE184
D	GLN202
D	LEU203
D	TYR204
D	ILE205
D	LYS215
D	ASP217
D	LEU262
D	THR263
D	ASN264
D	ASP346
D	LEU348
D	LEU358
D	GLU359
D	2TI601
D	MG604
D	MG605
D	HOH715
D	HOH717

site_id	AG2
Number of Residues	6
Details	binding site for residue GOL D 603

Chain	Residue
D	ARG68
D	TYR69
D	ASP70
D	SER367
D	THR368
D	HOH727

site_id	AG3
Number of Residues	5
Details	binding site for residue MG D 604

Chain	Residue
D	GLU359
D	ASN361
D	2TI601
D	ADP602
D	HOH717

site_id	AG4
Number of Residues	6
Details	binding site for residue MG D 605

Chain	Residue
D	ARG241
D	ASN264
D	ASP346
D	GLU359
D	2TI601
D	ADP602

site_id	AG5
Number of Residues	3
Details	binding site for residue GOL D 606

Chain	Residue
D	GLU301
D	ARG305
D	ARG445

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:32747782, ECO:0007744\|PDB:6VZT

Chain	Residue	Details
A	LYS174
C	GLN202
C	LYS215
C	THR263
D	LYS174
D	GLN202
D	LYS215
D	THR263
A	GLN202
A	LYS215
A	THR263
B	LYS174
B	GLN202
B	LYS215
B	THR263
C	LYS174

site_id	SWS_FT_FI2
Number of Residues	8
Details	BINDING: BINDING => ECO:0000305\|PubMed:32747782

Chain	Residue	Details
A	ARG180
A	ILE362
B	ARG180
B	ILE362
C	ARG180
C	ILE362
D	ARG180
D	ILE362

site_id	SWS_FT_FI3
Number of Residues	20
Details	BINDING: BINDING => ECO:0000305\|PubMed:32747782, ECO:0007744\|PDB:6VZU, ECO:0007744\|PDB:6VZW

Chain	Residue	Details
A	ARG241
B	LYS377
C	ARG241
C	TYR265
C	SER266
C	LYS283
C	LYS377
D	ARG241
D	TYR265
D	SER266
D	LYS283
A	TYR265
D	LYS377
A	SER266
A	LYS283
A	LYS377
B	ARG241
B	TYR265
B	SER266
B	LYS283

site_id	SWS_FT_FI4
Number of Residues	12
Details	BINDING: BINDING => ECO:0000269\|PubMed:32747782, ECO:0007744\|PDB:6VZU

Chain	Residue	Details
A	ASP346
D	ASP346
D	GLU359
D	ASN361
A	GLU359
A	ASN361
B	ASP346
B	GLU359
B	ASN361
C	ASP346
C	GLU359
C	ASN361

site_id	SWS_FT_FI5
Number of Residues	4
Details	SITE: Essential for specifying alpha-elongation versus initiation step of the polyglutamylase activity => ECO:0000269\|PubMed:32747782

Chain	Residue	Details
A	ARG180
B	ARG180
C	ARG180
D	ARG180

site_id	SWS_FT_FI6
Number of Residues	4
Details	SITE: Important for specifying alpha-elongation versus initiation step of the polyglutamylase activity => ECO:0000269\|PubMed:32747782

Chain	Residue	Details
A	ILE362
B	ILE362
C	ILE362
D	ILE362

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)