Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6VVO

Structure of the human clamp loader (Replication Factor C, RFC) bound to the sliding clamp (Proliferating Cell Nuclear Antigen, PCNA)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003689	molecular_function	DNA clamp loader activity
A	0005524	molecular_function	ATP binding
A	0005663	cellular_component	DNA replication factor C complex
A	0006260	biological_process	DNA replication
A	0006281	biological_process	DNA repair
A	0016887	molecular_function	ATP hydrolysis activity
B	0003677	molecular_function	DNA binding
B	0003689	molecular_function	DNA clamp loader activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005663	cellular_component	DNA replication factor C complex
B	0006260	biological_process	DNA replication
B	0006261	biological_process	DNA-templated DNA replication
B	0006281	biological_process	DNA repair
B	0016887	molecular_function	ATP hydrolysis activity
B	0017116	molecular_function	single-stranded DNA helicase activity
B	0019899	molecular_function	enzyme binding
B	0031390	cellular_component	Ctf18 RFC-like complex
B	0032508	biological_process	DNA duplex unwinding
B	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
C	0003677	molecular_function	DNA binding
C	0003689	molecular_function	DNA clamp loader activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005663	cellular_component	DNA replication factor C complex
C	0006260	biological_process	DNA replication
C	0006261	biological_process	DNA-templated DNA replication
C	0006281	biological_process	DNA repair
C	0016887	molecular_function	ATP hydrolysis activity
C	0017116	molecular_function	single-stranded DNA helicase activity
C	0019899	molecular_function	enzyme binding
C	0031390	cellular_component	Ctf18 RFC-like complex
C	0032508	biological_process	DNA duplex unwinding
C	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
D	0003677	molecular_function	DNA binding
D	0003689	molecular_function	DNA clamp loader activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005663	cellular_component	DNA replication factor C complex
D	0006260	biological_process	DNA replication
D	0006261	biological_process	DNA-templated DNA replication
D	0006271	biological_process	DNA strand elongation involved in DNA replication
D	0006281	biological_process	DNA repair
D	0016887	molecular_function	ATP hydrolysis activity
D	0017116	molecular_function	single-stranded DNA helicase activity
D	0031390	cellular_component	Ctf18 RFC-like complex
D	0031391	cellular_component	Elg1 RFC-like complex
D	0032508	biological_process	DNA duplex unwinding
D	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
E	0000731	biological_process	DNA synthesis involved in DNA repair
E	0003677	molecular_function	DNA binding
E	0003689	molecular_function	DNA clamp loader activity
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005663	cellular_component	DNA replication factor C complex
E	0006260	biological_process	DNA replication
E	0006261	biological_process	DNA-templated DNA replication
E	0006271	biological_process	DNA strand elongation involved in DNA replication
E	0006281	biological_process	DNA repair
E	0008094	molecular_function	ATP-dependent activity, acting on DNA
E	0016887	molecular_function	ATP hydrolysis activity
E	0017116	molecular_function	single-stranded DNA helicase activity
E	0031390	cellular_component	Ctf18 RFC-like complex
E	0032508	biological_process	DNA duplex unwinding
E	0046683	biological_process	response to organophosphorus
E	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
F	0000122	biological_process	negative regulation of transcription by RNA polymerase II
F	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
F	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
F	0000781	cellular_component	chromosome, telomeric region
F	0000785	cellular_component	chromatin
F	0001673	cellular_component	male germ cell nucleus
F	0003677	molecular_function	DNA binding
F	0003682	molecular_function	chromatin binding
F	0003684	molecular_function	damaged DNA binding
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005652	cellular_component	nuclear lamina
F	0005654	cellular_component	nucleoplasm
F	0005657	cellular_component	replication fork
F	0005813	cellular_component	centrosome
F	0006260	biological_process	DNA replication
F	0006272	biological_process	leading strand elongation
F	0006275	biological_process	regulation of DNA replication
F	0006281	biological_process	DNA repair
F	0006287	biological_process	base-excision repair, gap-filling
F	0006298	biological_process	mismatch repair
F	0006979	biological_process	response to oxidative stress
F	0007507	biological_process	heart development
F	0016604	cellular_component	nuclear body
F	0019899	molecular_function	enzyme binding
F	0019985	biological_process	translesion synthesis
F	0030331	molecular_function	nuclear estrogen receptor binding
F	0030337	molecular_function	DNA polymerase processivity factor activity
F	0030855	biological_process	epithelial cell differentiation
F	0030894	cellular_component	replisome
F	0030971	molecular_function	receptor tyrosine kinase binding
F	0031297	biological_process	replication fork processing
F	0032077	biological_process	positive regulation of deoxyribonuclease activity
F	0032139	molecular_function	dinucleotide insertion or deletion binding
F	0032355	biological_process	response to estradiol
F	0032405	molecular_function	MutLalpha complex binding
F	0033993	biological_process	response to lipid
F	0034644	biological_process	cellular response to UV
F	0035035	molecular_function	histone acetyltransferase binding
F	0042802	molecular_function	identical protein binding
F	0043596	cellular_component	nuclear replication fork
F	0043626	cellular_component	PCNA complex
F	0044849	biological_process	estrous cycle
F	0044877	molecular_function	protein-containing complex binding
F	0045739	biological_process	positive regulation of DNA repair
F	0045740	biological_process	positive regulation of DNA replication
F	0046686	biological_process	response to cadmium ion
F	0070062	cellular_component	extracellular exosome
F	0070182	molecular_function	DNA polymerase binding
F	0070301	biological_process	cellular response to hydrogen peroxide
F	0070557	cellular_component	PCNA-p21 complex
F	0071466	biological_process	cellular response to xenobiotic stimulus
F	0071548	biological_process	response to dexamethasone
F	0097421	biological_process	liver regeneration
F	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
F	1902065	biological_process	response to L-glutamate
F	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication
G	0000122	biological_process	negative regulation of transcription by RNA polymerase II
G	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
G	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
G	0000781	cellular_component	chromosome, telomeric region
G	0000785	cellular_component	chromatin
G	0001673	cellular_component	male germ cell nucleus
G	0003677	molecular_function	DNA binding
G	0003682	molecular_function	chromatin binding
G	0003684	molecular_function	damaged DNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005652	cellular_component	nuclear lamina
G	0005654	cellular_component	nucleoplasm
G	0005657	cellular_component	replication fork
G	0005813	cellular_component	centrosome
G	0006260	biological_process	DNA replication
G	0006272	biological_process	leading strand elongation
G	0006275	biological_process	regulation of DNA replication
G	0006281	biological_process	DNA repair
G	0006287	biological_process	base-excision repair, gap-filling
G	0006298	biological_process	mismatch repair
G	0006979	biological_process	response to oxidative stress
G	0007507	biological_process	heart development
G	0016604	cellular_component	nuclear body
G	0019899	molecular_function	enzyme binding
G	0019985	biological_process	translesion synthesis
G	0030331	molecular_function	nuclear estrogen receptor binding
G	0030337	molecular_function	DNA polymerase processivity factor activity
G	0030855	biological_process	epithelial cell differentiation
G	0030894	cellular_component	replisome
G	0030971	molecular_function	receptor tyrosine kinase binding
G	0031297	biological_process	replication fork processing
G	0032077	biological_process	positive regulation of deoxyribonuclease activity
G	0032139	molecular_function	dinucleotide insertion or deletion binding
G	0032355	biological_process	response to estradiol
G	0032405	molecular_function	MutLalpha complex binding
G	0033993	biological_process	response to lipid
G	0034644	biological_process	cellular response to UV
G	0035035	molecular_function	histone acetyltransferase binding
G	0042802	molecular_function	identical protein binding
G	0043596	cellular_component	nuclear replication fork
G	0043626	cellular_component	PCNA complex
G	0044849	biological_process	estrous cycle
G	0044877	molecular_function	protein-containing complex binding
G	0045739	biological_process	positive regulation of DNA repair
G	0045740	biological_process	positive regulation of DNA replication
G	0046686	biological_process	response to cadmium ion
G	0070062	cellular_component	extracellular exosome
G	0070182	molecular_function	DNA polymerase binding
G	0070301	biological_process	cellular response to hydrogen peroxide
G	0070557	cellular_component	PCNA-p21 complex
G	0071466	biological_process	cellular response to xenobiotic stimulus
G	0071548	biological_process	response to dexamethasone
G	0097421	biological_process	liver regeneration
G	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
G	1902065	biological_process	response to L-glutamate
G	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication
H	0000122	biological_process	negative regulation of transcription by RNA polymerase II
H	0000307	cellular_component	cyclin-dependent protein kinase holoenzyme complex
H	0000701	molecular_function	purine-specific mismatch base pair DNA N-glycosylase activity
H	0000781	cellular_component	chromosome, telomeric region
H	0000785	cellular_component	chromatin
H	0001673	cellular_component	male germ cell nucleus
H	0003677	molecular_function	DNA binding
H	0003682	molecular_function	chromatin binding
H	0003684	molecular_function	damaged DNA binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005652	cellular_component	nuclear lamina
H	0005654	cellular_component	nucleoplasm
H	0005657	cellular_component	replication fork
H	0005813	cellular_component	centrosome
H	0006260	biological_process	DNA replication
H	0006272	biological_process	leading strand elongation
H	0006275	biological_process	regulation of DNA replication
H	0006281	biological_process	DNA repair
H	0006287	biological_process	base-excision repair, gap-filling
H	0006298	biological_process	mismatch repair
H	0006979	biological_process	response to oxidative stress
H	0007507	biological_process	heart development
H	0016604	cellular_component	nuclear body
H	0019899	molecular_function	enzyme binding
H	0019985	biological_process	translesion synthesis
H	0030331	molecular_function	nuclear estrogen receptor binding
H	0030337	molecular_function	DNA polymerase processivity factor activity
H	0030855	biological_process	epithelial cell differentiation
H	0030894	cellular_component	replisome
H	0030971	molecular_function	receptor tyrosine kinase binding
H	0031297	biological_process	replication fork processing
H	0032077	biological_process	positive regulation of deoxyribonuclease activity
H	0032139	molecular_function	dinucleotide insertion or deletion binding
H	0032355	biological_process	response to estradiol
H	0032405	molecular_function	MutLalpha complex binding
H	0033993	biological_process	response to lipid
H	0034644	biological_process	cellular response to UV
H	0035035	molecular_function	histone acetyltransferase binding
H	0042802	molecular_function	identical protein binding
H	0043596	cellular_component	nuclear replication fork
H	0043626	cellular_component	PCNA complex
H	0044849	biological_process	estrous cycle
H	0044877	molecular_function	protein-containing complex binding
H	0045739	biological_process	positive regulation of DNA repair
H	0045740	biological_process	positive regulation of DNA replication
H	0046686	biological_process	response to cadmium ion
H	0070062	cellular_component	extracellular exosome
H	0070182	molecular_function	DNA polymerase binding
H	0070301	biological_process	cellular response to hydrogen peroxide
H	0070557	cellular_component	PCNA-p21 complex
H	0071466	biological_process	cellular response to xenobiotic stimulus
H	0071548	biological_process	response to dexamethasone
H	0097421	biological_process	liver regeneration
H	1900264	biological_process	positive regulation of DNA-directed DNA polymerase activity
H	1902065	biological_process	response to L-glutamate
H	1902990	biological_process	mitotic telomere maintenance via semi-conservative replication

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	binding site for residue MG A 1201

Chain	Residue
A	THR658
A	AGS1202

site_id	AC2
Number of Residues	16
Details	binding site for residue AGS A 1202

Chain	Residue
A	LYS657
A	THR658
A	THR659
A	ASN753
A	ARG812
A	MG1201
B	ARG155
B	GLU159
B	ARG184
A	VAL587
A	ILE598
A	ILE599
A	GLY600
A	GLY654
A	VAL655
A	GLY656

site_id	AC3
Number of Residues	3
Details	binding site for residue MG B 401

Chain	Residue
B	THR83
B	AGS402
C	ARG139

site_id	AC4
Number of Residues	19
Details	binding site for residue AGS B 402

Chain	Residue
B	VAL39
B	TYR42
B	ARG43
B	ILE50
B	VAL51
B	GLY52
B	PRO78
B	GLY79
B	THR80
B	GLY81
B	LYS82
B	THR83
B	THR84
B	ASN172
B	ARG201
B	MET229
B	ARG230
B	MG401
C	ARG139

site_id	AC5
Number of Residues	3
Details	binding site for residue MG C 401

Chain	Residue
C	THR67
C	ASP125
C	AGS402

site_id	AC6
Number of Residues	17
Details	binding site for residue AGS C 402

Chain	Residue
C	VAL23
C	TYR26
C	ARG27
C	PRO28
C	LEU34
C	ILE35
C	GLY63
C	THR64
C	GLY65
C	LYS66
C	THR67
C	SER68
C	ASN156
C	ARG185
C	ARG214
C	MG401
D	ARG165

site_id	AC7
Number of Residues	3
Details	binding site for residue MG D 401

Chain	Residue
D	THR85
D	ASP150
D	AGS402

site_id	AC8
Number of Residues	16
Details	binding site for residue AGS D 402

Chain	Residue
D	VAL41
D	TYR44
D	ARG45
D	VAL52
D	ALA53
D	GLN55
D	GLY81
D	THR82
D	GLY83
D	LYS84
D	THR85
D	SER86
D	ARG210
D	LEU238
D	ARG239
D	MG401

site_id	AC9
Number of Residues	17
Details	binding site for residue ADP E 2000

Chain	Residue
E	VAL5
E	ARG9
E	PRO10
E	ASP17
E	TYR18
E	SER44
E	GLY45
E	ALA46
E	GLY47
E	LYS48
E	LYS49
E	THR50
E	ARG51
E	VAL195
E	LEU223
E	ARG224
E	LEU227

Functional Information from PROSITE/UniProt

site_id	PS00293
Number of Residues	19
Details	PCNA_2 Proliferating cell nuclear antigen signature 2. RCDRnlaMgvnLtSMsKIL

Chain	Residue	Details
F	ARG61-LEU79

site_id	PS01251
Number of Residues	24
Details	PCNA_1 Proliferating cell nuclear antigen signature 1. GVnLqSMDsSHVsLVqLtLrsegF

Chain	Residue	Details
F	GLY34-PHE57

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	57
Details	DNA_BIND: DNA_BIND => ECO:0000255

Chain	Residue	Details
F	ARG61-LYS80
G	ARG61-LYS80
H	ARG61-LYS80

site_id	SWS_FT_FI2
Number of Residues	3
Details	MOD_RES: N6-acetyllysine => ECO:0000269\|PubMed:19419956

Chain	Residue	Details
F	LYS14
G	LYS14
H	LYS14

site_id	SWS_FT_FI3
Number of Residues	9
Details	MOD_RES: N6-acetyllysine => ECO:0007744\|PubMed:19608861

Chain	Residue	Details
F	LYS77
F	LYS80
F	LYS248
G	LYS77
G	LYS80
G	LYS248
H	LYS77
H	LYS80
H	LYS248

site_id	SWS_FT_FI4
Number of Residues	3
Details	MOD_RES: Phosphotyrosine; by EGFR => ECO:0000269\|PubMed:17115032

Chain	Residue	Details
F	TYR211
G	TYR211
H	TYR211

site_id	SWS_FT_FI5
Number of Residues	3
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate => ECO:0000269\|PubMed:17108083, ECO:0000269\|PubMed:17130289

Chain	Residue	Details
F	LYS164
G	LYS164
H	LYS164

site_id	SWS_FT_FI6
Number of Residues	6
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
F	LYS254
G	LYS254
H	LYS254

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)