6V65

Crystal structure of KRAS(GMPPNP)-NF1(GRD)-SPRED1 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
B	0043087	biological_process	regulation of GTPase activity
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
C	0007165	biological_process	signal transduction
C	0016020	cellular_component	membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	binding site for residue ZN A 201

Chain	Residue
A	HIS49
A	CYS55

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site_id	AC2
Number of Residues	2
Details	binding site for residue FMT B 1601

Chain	Residue
B	ARG1441
B	ASN1444

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site_id	AC3
Number of Residues	20
Details	binding site for residue GNP C 501

Chain	Residue
C	PHE28
C	VAL29
C	ASP30
C	GLU31
C	PRO34
C	THR35
C	GLY60
C	ASN116
C	LYS117
C	ASP119
C	LEU120
C	SER145
C	ALA146
C	LYS147
C	MG502
C	GLY13
C	GLY15
C	LYS16
C	SER17
C	ALA18

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site_id	AC4
Number of Residues	4
Details	binding site for residue MG C 502

Chain	Residue
C	SER17
C	THR35
C	ASP57
C	GNP501

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Functional Information from PROSITE/UniProt

site_id	PS00509
Number of Residues	15
Details	RAS_GTPASE_ACTIV_1 Ras GTPase-activating proteins domain signature. SaMFLRFINPAIVSP

Chain	Residue	Details
B	SER1386-PRO1400

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:22431598, ECO:0000269|PubMed:22566140

Chain	Residue	Details
C	GLY10
C	VAL29
C	ALA59
C	ASN116

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site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N-acetylmethionine; in GTPase KRas; alternate => ECO:0000269|Ref.17

Chain	Residue	Details
C	MET1

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site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: N-acetylthreonine; in GTPase KRas, N-terminally processed => ECO:0000269|Ref.17

Chain	Residue	Details
C	THR2

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:22711838

Chain	Residue	Details
C	LYS104

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site_id	SWS_FT_FI5
Number of Residues	1
Details	CARBOHYD: (Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL => ECO:0000269|PubMed:19744486

Chain	Residue	Details
C	THR35

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