6UKT
Cryo-EM structure of mammalian Ric-8A:Galpha(i):nanobody complex
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0001701 | biological_process | in utero embryonic development |
| A | 0001944 | biological_process | vasculature development |
| A | 0001965 | molecular_function | G-protein alpha-subunit binding |
| A | 0005085 | molecular_function | guanyl-nucleotide exchange factor activity |
| A | 0005096 | molecular_function | GTPase activator activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0005938 | cellular_component | cell cortex |
| A | 0006457 | biological_process | protein folding |
| A | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| A | 0007193 | biological_process | adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| A | 0007369 | biological_process | gastrulation |
| A | 0008542 | biological_process | visual learning |
| A | 0009416 | biological_process | response to light stimulus |
| A | 0016020 | cellular_component | membrane |
| A | 0042074 | biological_process | cell migration involved in gastrulation |
| A | 0044183 | molecular_function | protein folding chaperone |
| A | 0070586 | biological_process | cell-cell adhesion involved in gastrulation |
| A | 0071711 | biological_process | basement membrane organization |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0000287 | molecular_function | magnesium ion binding |
| B | 0001664 | molecular_function | G protein-coupled receptor binding |
| B | 0003924 | molecular_function | GTPase activity |
| B | 0003925 | molecular_function | G protein activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005525 | molecular_function | GTP binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005813 | cellular_component | centrosome |
| B | 0005829 | cellular_component | cytosol |
| B | 0005834 | cellular_component | heterotrimeric G-protein complex |
| B | 0005856 | cellular_component | cytoskeleton |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0005938 | cellular_component | cell cortex |
| B | 0007165 | biological_process | signal transduction |
| B | 0007186 | biological_process | G protein-coupled receptor signaling pathway |
| B | 0007188 | biological_process | adenylate cyclase-modulating G protein-coupled receptor signaling pathway |
| B | 0007193 | biological_process | adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| B | 0007198 | biological_process | adenylate cyclase-inhibiting serotonin receptor signaling pathway |
| B | 0010854 | molecular_function | adenylate cyclase regulator activity |
| B | 0010855 | molecular_function | adenylate cyclase inhibitor activity |
| B | 0016020 | cellular_component | membrane |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0019001 | molecular_function | guanyl nucleotide binding |
| B | 0019003 | molecular_function | GDP binding |
| B | 0030496 | cellular_component | midbody |
| B | 0031683 | molecular_function | G-protein beta/gamma-subunit complex binding |
| B | 0031749 | molecular_function | D2 dopamine receptor binding |
| B | 0031821 | molecular_function | G protein-coupled serotonin receptor binding |
| B | 0032794 | molecular_function | GTPase activating protein binding |
| B | 0032991 | cellular_component | protein-containing complex |
| B | 0034695 | biological_process | response to prostaglandin E |
| B | 0045542 | biological_process | positive regulation of cholesterol biosynthetic process |
| B | 0046676 | biological_process | negative regulation of insulin secretion |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0050805 | biological_process | negative regulation of synaptic transmission |
| B | 0051301 | biological_process | cell division |
| B | 0060236 | biological_process | regulation of mitotic spindle organization |
| B | 0072678 | biological_process | T cell migration |
| B | 0098794 | cellular_component | postsynapse |
| B | 0098978 | cellular_component | glutamatergic synapse |
| B | 0099645 | biological_process | neurotransmitter receptor localization to postsynaptic specialization membrane |
| B | 1904322 | biological_process | cellular response to forskolin |
| B | 1904778 | biological_process | positive regulation of protein localization to cell cortex |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine; by CK2","evidences":[{"source":"PubMed","id":"29844055","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32103024","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32126208","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphothreonine; by CK2","evidences":[{"source":"PubMed","id":"29844055","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32103024","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"32126208","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"UniProtKB","id":"Q3TIR3","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 9 |
| Details | Region: {"description":"G3 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 7 |
| Details | Region: {"description":"G4 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 5 |
| Details | Region: {"description":"G5 motif","evidences":[{"source":"PROSITE-ProRule","id":"PRU01230","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 12 |
| Details | Binding site: {"evidences":[{"source":"PDB","id":"1AS0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FFA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N0D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAQ","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"25037222","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9705312","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PDB","id":"1AS0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N0D","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"19703466","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"25037222","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9705312","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1BH2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI11 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"PDB","id":"1AS0","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"1GIL","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3FFA","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N0D","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAO","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4PAQ","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 4 |
| Details | M-CSA 533 |
| Chain | Residue | Details |
| B | GLU43 | electrostatic stabiliser |
| B | THR48 | electrostatic stabiliser |
| B | ASP200 | electrostatic stabiliser |
| B | GLN204 | electrostatic stabiliser |
