6UKD

Co-complex of S. pyogenes 10782 streptopain bound with a nitrile-based specific covalent inhibitor

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004197	molecular_function	cysteine-type endopeptidase activity
A	0005576	cellular_component	extracellular region
A	0006508	biological_process	proteolysis
A	0008233	molecular_function	peptidase activity
A	0008234	molecular_function	cysteine-type peptidase activity
A	0016787	molecular_function	hydrolase activity
A	0030430	cellular_component	host cell cytoplasm
A	0034050	biological_process	symbiont-induced defense-related programmed cell death
A	0035893	biological_process	obsolete suppression of platelet aggregation in another organism
A	0042783	biological_process	symbiont-mediated evasion of host immune response
A	0043655	cellular_component	host extracellular space
A	0044164	cellular_component	host cell cytosol
A	0052035	biological_process	obsolete positive regulation by symbiont of host inflammatory response
A	0090729	molecular_function	toxin activity
A	0140321	biological_process	symbiont-mediated suppression of host autophagy

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	binding site for residue Q9D A 501

Chain	Residue
A	GLN162
A	GLY333
A	VAL334
A	GLY339
A	HIS340
A	HOH689
A	HOH764
A	HOH835
A	CYS192
A	VAL193
A	ASN208
A	SER280
A	GLY281
A	SER282
A	ALA283
A	GLN332

---

site_id	AC2
Number of Residues	8
Details	binding site for residue NO3 A 502

Chain	Residue
A	ASN164
A	PRO165
A	LYS258
A	PHE367
A	ASN373
A	SER375
A	HOH608
A	HOH702

---

site_id	AC3
Number of Residues	6
Details	binding site for residue NO3 A 503

Chain	Residue
A	GLY335
A	LYS336
A	GLY384
A	GLY385
A	ASN387
A	HOH603

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	22
Details	Region: {"description":"C-terminal active site loop","evidences":[{"source":"PubMed","id":"22645124","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	Active site: {"description":"Nucleophile","evidences":[{"source":"PubMed","id":"19712682","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22645124","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"8675287","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PubMed","id":"19712682","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	3
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22645124","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"4D8E","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	Binding site: {"description":"covalent","evidences":[{"source":"PubMed","id":"19712682","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22645124","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2UZJ","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4D8E","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22645124","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4D8I","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"Cysteine methyl disulfide; in zymogen form","evidences":[{"source":"PubMed","id":"6381494","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 598

Chain	Residue	Details
A	CYS192
A	HIS340	proton shuttle (general acid/base)
A	TRP357

---