6UKD

Co-complex of S. pyogenes 10782 streptopain bound with a nitrile-based specific covalent inhibitor

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004197	molecular_function	cysteine-type endopeptidase activity
A	0005576	cellular_component	extracellular region
A	0006508	biological_process	proteolysis
A	0008234	molecular_function	cysteine-type peptidase activity
A	0030430	cellular_component	host cell cytoplasm
A	0034050	biological_process	symbiont-induced defense-related programmed cell death
A	0042783	biological_process	evasion of host immune response
A	0043655	cellular_component	host extracellular space
A	0044164	cellular_component	host cell cytosol
A	0052035	biological_process	obsolete positive regulation by symbiont of host inflammatory response
A	0090729	molecular_function	toxin activity
A	0140321	biological_process	symbiont-mediated suppression of host autophagy

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	binding site for residue Q9D A 501

Chain	Residue
A	GLN162
A	GLY333
A	VAL334
A	GLY339
A	HIS340
A	HOH689
A	HOH764
A	HOH835
A	CYS192
A	VAL193
A	ASN208
A	SER280
A	GLY281
A	SER282
A	ALA283
A	GLN332

---

site_id	AC2
Number of Residues	8
Details	binding site for residue NO3 A 502

Chain	Residue
A	ASN164
A	PRO165
A	LYS258
A	PHE367
A	ASN373
A	SER375
A	HOH608
A	HOH702

---

site_id	AC3
Number of Residues	6
Details	binding site for residue NO3 A 503

Chain	Residue
A	GLY335
A	LYS336
A	GLY384
A	GLY385
A	ASN387
A	HOH603

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:19712682, ECO:0000269|PubMed:22645124, ECO:0000305|PubMed:8675287

Chain	Residue	Details
A	CYS192

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	ACT_SITE: Proton acceptor => ECO:0000269|PubMed:19712682

Chain	Residue	Details
A	HIS340

---

site_id	SWS_FT_FI3
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:22645124, ECO:0007744|PDB:4D8E

Chain	Residue	Details
A	GLN162
A	SER282
A	HIS340

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	BINDING: covalent => ECO:0000269|PubMed:19712682, ECO:0000269|PubMed:22645124, ECO:0007744|PDB:2UZJ, ECO:0007744|PDB:4D8E

Chain	Residue	Details
A	CYS192

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	BINDING: BINDING => ECO:0000305|PubMed:22645124, ECO:0007744|PDB:4D8I

Chain	Residue	Details
A	GLY339

---

site_id	SWS_FT_FI6
Number of Residues	5
Details	SITE: Cleavage; by autolysis => ECO:0000269|PubMed:10429198

Chain	Residue	Details
A	LYS53
A	ASN68
A	LYS129
A	ALA139
A	LYS145

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Cysteine methyl disulfide; in zymogen form => ECO:0000269|PubMed:6381494

Chain	Residue	Details
A	CYS192

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	3
Details	M-CSA 598

Chain	Residue	Details
A	CYS192
A	HIS340	proton shuttle (general acid/base)
A	TRP357

---