Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6SH1

Crystal structure of substrate-free human neprilysin E584D.

This is a non-PDB format compatible entry.

Functional Information from GO Data

Chain	GOid	namespace	contents
AAA	0001786	molecular_function	phosphatidylserine binding
AAA	0001822	biological_process	kidney development
AAA	0001890	biological_process	placenta development
AAA	0004175	molecular_function	endopeptidase activity
AAA	0004222	molecular_function	metalloendopeptidase activity
AAA	0005515	molecular_function	protein binding
AAA	0005737	cellular_component	cytoplasm
AAA	0005769	cellular_component	early endosome
AAA	0005802	cellular_component	trans-Golgi network
AAA	0005886	cellular_component	plasma membrane
AAA	0005903	cellular_component	brush border
AAA	0005925	cellular_component	focal adhesion
AAA	0006508	biological_process	proteolysis
AAA	0006518	biological_process	peptide metabolic process
AAA	0007611	biological_process	learning or memory
AAA	0008021	cellular_component	synaptic vesicle
AAA	0008233	molecular_function	peptidase activity
AAA	0008237	molecular_function	metallopeptidase activity
AAA	0008238	molecular_function	exopeptidase activity
AAA	0008270	molecular_function	zinc ion binding
AAA	0009986	cellular_component	cell surface
AAA	0010814	biological_process	substance P catabolic process
AAA	0010815	biological_process	bradykinin catabolic process
AAA	0016020	cellular_component	membrane
AAA	0016485	biological_process	protein processing
AAA	0016787	molecular_function	hydrolase activity
AAA	0019233	biological_process	sensory perception of pain
AAA	0030163	biological_process	protein catabolic process
AAA	0030324	biological_process	lung development
AAA	0030424	cellular_component	axon
AAA	0030425	cellular_component	dendrite
AAA	0030667	cellular_component	secretory granule membrane
AAA	0031410	cellular_component	cytoplasmic vesicle
AAA	0042277	molecular_function	peptide binding
AAA	0042447	biological_process	hormone catabolic process
AAA	0042803	molecular_function	protein homodimerization activity
AAA	0043025	cellular_component	neuronal cell body
AAA	0043627	biological_process	response to estrogen
AAA	0044306	cellular_component	neuron projection terminus
AAA	0045121	cellular_component	membrane raft
AAA	0045202	cellular_component	synapse
AAA	0046449	biological_process	creatinine metabolic process
AAA	0046872	molecular_function	metal ion binding
AAA	0050435	biological_process	amyloid-beta metabolic process
AAA	0050769	biological_process	positive regulation of neurogenesis
AAA	0061837	biological_process	neuropeptide processing
AAA	0070012	molecular_function	oligopeptidase activity
AAA	0070062	cellular_component	extracellular exosome
AAA	0071345	biological_process	cellular response to cytokine stimulus
AAA	0071492	biological_process	cellular response to UV-A
AAA	0071493	biological_process	cellular response to UV-B
AAA	0090399	biological_process	replicative senescence
AAA	0097242	biological_process	amyloid-beta clearance
AAA	0098793	cellular_component	presynapse
AAA	0150094	biological_process	amyloid-beta clearance by cellular catabolic process
AAA	1900273	biological_process	positive regulation of long-term synaptic potentiation
AAA	1901612	molecular_function	cardiolipin binding
CCC	0001786	molecular_function	phosphatidylserine binding
CCC	0001822	biological_process	kidney development
CCC	0001890	biological_process	placenta development
CCC	0004175	molecular_function	endopeptidase activity
CCC	0004222	molecular_function	metalloendopeptidase activity
CCC	0005515	molecular_function	protein binding
CCC	0005737	cellular_component	cytoplasm
CCC	0005769	cellular_component	early endosome
CCC	0005802	cellular_component	trans-Golgi network
CCC	0005886	cellular_component	plasma membrane
CCC	0005903	cellular_component	brush border
CCC	0005925	cellular_component	focal adhesion
CCC	0006508	biological_process	proteolysis
CCC	0006518	biological_process	peptide metabolic process
CCC	0007611	biological_process	learning or memory
CCC	0008021	cellular_component	synaptic vesicle
CCC	0008233	molecular_function	peptidase activity
CCC	0008237	molecular_function	metallopeptidase activity
CCC	0008238	molecular_function	exopeptidase activity
CCC	0008270	molecular_function	zinc ion binding
CCC	0009986	cellular_component	cell surface
CCC	0010814	biological_process	substance P catabolic process
CCC	0010815	biological_process	bradykinin catabolic process
CCC	0016020	cellular_component	membrane
CCC	0016485	biological_process	protein processing
CCC	0016787	molecular_function	hydrolase activity
CCC	0019233	biological_process	sensory perception of pain
CCC	0030163	biological_process	protein catabolic process
CCC	0030324	biological_process	lung development
CCC	0030424	cellular_component	axon
CCC	0030425	cellular_component	dendrite
CCC	0030667	cellular_component	secretory granule membrane
CCC	0031410	cellular_component	cytoplasmic vesicle
CCC	0042277	molecular_function	peptide binding
CCC	0042447	biological_process	hormone catabolic process
CCC	0042803	molecular_function	protein homodimerization activity
CCC	0043025	cellular_component	neuronal cell body
CCC	0043627	biological_process	response to estrogen
CCC	0044306	cellular_component	neuron projection terminus
CCC	0045121	cellular_component	membrane raft
CCC	0045202	cellular_component	synapse
CCC	0046449	biological_process	creatinine metabolic process
CCC	0046872	molecular_function	metal ion binding
CCC	0050435	biological_process	amyloid-beta metabolic process
CCC	0050769	biological_process	positive regulation of neurogenesis
CCC	0061837	biological_process	neuropeptide processing
CCC	0070012	molecular_function	oligopeptidase activity
CCC	0070062	cellular_component	extracellular exosome
CCC	0071345	biological_process	cellular response to cytokine stimulus
CCC	0071492	biological_process	cellular response to UV-A
CCC	0071493	biological_process	cellular response to UV-B
CCC	0090399	biological_process	replicative senescence
CCC	0097242	biological_process	amyloid-beta clearance
CCC	0098793	cellular_component	presynapse
CCC	0150094	biological_process	amyloid-beta clearance by cellular catabolic process
CCC	1900273	biological_process	positive regulation of long-term synaptic potentiation
CCC	1901612	molecular_function	cardiolipin binding

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: ACT_SITE => ECO:0000255\|PROSITE-ProRule:PRU01233, ECO:0000255\|PROSITE-ProRule:PRU10095

Chain	Residue	Details
AAA	ASP584
CCC	ASP584

site_id	SWS_FT_FI2
Number of Residues	2
Details	ACT_SITE: Proton donor => ECO:0000255\|PROSITE-ProRule:PRU01233, ECO:0000269\|PubMed:8168535

Chain	Residue	Details
AAA	ASP650
CCC	ASP650

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P07861

Chain	Residue	Details
AAA	ARG102
CCC	ARG102

site_id	SWS_FT_FI4
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU01233

Chain	Residue	Details
AAA	HIS583
AAA	HIS587
AAA	GLU646
CCC	HIS583
CCC	HIS587
CCC	GLU646

site_id	SWS_FT_FI5
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:10669592, ECO:0000269\|PubMed:12754519, ECO:0000269\|PubMed:14747736, ECO:0000269\|PubMed:17704566

Chain	Residue	Details
AAA	ASN144
CCC	ASN144

site_id	SWS_FT_FI6
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:12754519

Chain	Residue	Details
AAA	ASN284
CCC	ASN284

site_id	SWS_FT_FI7
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:10669592, ECO:0000269\|PubMed:14747736, ECO:0000269\|PubMed:17704566

Chain	Residue	Details
AAA	ASN324
CCC	ASN324

site_id	SWS_FT_FI8
Number of Residues	2
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269\|PubMed:10669592, ECO:0000269\|PubMed:14747736, ECO:0000269\|PubMed:17704566, ECO:0000269\|PubMed:22766194

Chain	Residue	Details
AAA	ASN627
CCC	ASN627

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	7
Details	M-CSA 623

Chain	Residue	Details
AAA	HIS583	metal ligand
AAA	ASP584	proton shuttle (general acid/base)
AAA	HIS587	metal ligand
AAA	GLU646	metal ligand
AAA	ASP650	electrostatic stabiliser
AAA	HIS711	electrostatic stabiliser
AAA	ARG717	electrostatic stabiliser

site_id	MCSA2
Number of Residues	7
Details	M-CSA 623

Chain	Residue	Details
CCC	HIS583	metal ligand
CCC	ASP584	proton shuttle (general acid/base)
CCC	HIS587	metal ligand
CCC	GLU646	metal ligand
CCC	ASP650	electrostatic stabiliser
CCC	HIS711	electrostatic stabiliser
CCC	ARG717	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)