Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6RIE

Structure of Vaccinia Virus DNA-dependent RNA polymerase co-transcriptional capping complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000428	cellular_component	DNA-directed RNA polymerase complex
A	0003677	molecular_function	DNA binding
A	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
A	0006351	biological_process	DNA-templated transcription
A	0016779	molecular_function	nucleotidyltransferase activity
A	0034062	molecular_function	5'-3' RNA polymerase activity
A	0044423	cellular_component	virion component
B	0000428	cellular_component	DNA-directed RNA polymerase complex
B	0001054	molecular_function	RNA polymerase I activity
B	0003677	molecular_function	DNA binding
B	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
B	0006351	biological_process	DNA-templated transcription
B	0006360	biological_process	transcription by RNA polymerase I
B	0016779	molecular_function	nucleotidyltransferase activity
B	0032549	molecular_function	ribonucleoside binding
B	0034062	molecular_function	5'-3' RNA polymerase activity
B	0044423	cellular_component	virion component
B	0046872	molecular_function	metal ion binding
C	0000428	cellular_component	DNA-directed RNA polymerase complex
C	0003677	molecular_function	DNA binding
C	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
C	0016779	molecular_function	nucleotidyltransferase activity
C	0019083	biological_process	viral transcription
C	0034062	molecular_function	5'-3' RNA polymerase activity
C	0044423	cellular_component	virion component
E	0000428	cellular_component	DNA-directed RNA polymerase complex
E	0003677	molecular_function	DNA binding
E	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
E	0016779	molecular_function	nucleotidyltransferase activity
E	0019083	biological_process	viral transcription
E	0034062	molecular_function	5'-3' RNA polymerase activity
E	0039695	biological_process	DNA-templated viral transcription
E	0044423	cellular_component	virion component
F	0000428	cellular_component	DNA-directed RNA polymerase complex
F	0003677	molecular_function	DNA binding
F	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
F	0006351	biological_process	DNA-templated transcription
F	0016779	molecular_function	nucleotidyltransferase activity
F	0034062	molecular_function	5'-3' RNA polymerase activity
F	0044423	cellular_component	virion component
G	0000428	cellular_component	DNA-directed RNA polymerase complex
G	0003677	molecular_function	DNA binding
G	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
G	0016779	molecular_function	nucleotidyltransferase activity
G	0019083	biological_process	viral transcription
G	0034062	molecular_function	5'-3' RNA polymerase activity
G	0044423	cellular_component	virion component
J	0000428	cellular_component	DNA-directed RNA polymerase complex
J	0003677	molecular_function	DNA binding
J	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
J	0006351	biological_process	DNA-templated transcription
J	0016779	molecular_function	nucleotidyltransferase activity
J	0034062	molecular_function	5'-3' RNA polymerase activity
J	0044423	cellular_component	virion component
L	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
L	0005515	molecular_function	protein binding
L	0006353	biological_process	DNA-templated transcription termination
L	0006370	biological_process	7-methylguanosine mRNA capping
L	0044423	cellular_component	virion component
L	0106005	biological_process	RNA 5'-cap (guanine-N7)-methylation
O	0003723	molecular_function	RNA binding
O	0004482	molecular_function	mRNA 5'-cap (guanine-N7-)-methyltransferase activity
O	0004484	molecular_function	mRNA guanylyltransferase activity
O	0004651	molecular_function	polynucleotide 5'-phosphatase activity
O	0005515	molecular_function	protein binding
O	0005525	molecular_function	GTP binding
O	0006353	biological_process	DNA-templated transcription termination
O	0006370	biological_process	7-methylguanosine mRNA capping
O	0008168	molecular_function	methyltransferase activity
O	0016779	molecular_function	nucleotidyltransferase activity
O	0016787	molecular_function	hydrolase activity
O	0032259	biological_process	methylation
O	0044423	cellular_component	virion component
O	0046872	molecular_function	metal ion binding
O	0050355	molecular_function	inorganic triphosphate phosphatase activity
O	0106005	biological_process	RNA 5'-cap (guanine-N7)-methylation
O	0140818	molecular_function	mRNA 5'-triphosphate monophosphatase activity
S	0000428	cellular_component	DNA-directed RNA polymerase complex
S	0003676	molecular_function	nucleic acid binding
S	0003677	molecular_function	DNA binding
S	0003899	molecular_function	DNA-directed 5'-3' RNA polymerase activity
S	0006351	biological_process	DNA-templated transcription
S	0008270	molecular_function	zinc ion binding
S	0016779	molecular_function	nucleotidyltransferase activity
S	0030430	cellular_component	host cell cytoplasm
S	0034062	molecular_function	5'-3' RNA polymerase activity
S	0044423	cellular_component	virion component
S	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue MG A 1301

Chain	Residue
A	ASP415
A	ASP417
A	ASP419
P	G30

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN A 1302

Chain	Residue
A	CYS49
A	CYS52
A	CYS59
A	HIS62

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN A 1303

Chain	Residue
A	CYS93
A	CYS130
A	CYS135
A	CYS90

site_id	AC4
Number of Residues	4
Details	binding site for residue ZN B 1201

Chain	Residue
B	CYS1087
B	CYS1090
B	CYS1103
B	CYS1106

site_id	AC5
Number of Residues	4
Details	binding site for residue ZN J 101

Chain	Residue
J	CYS7
J	CYS10
J	CYS39
J	CYS40

site_id	AC6
Number of Residues	17
Details	binding site for residue SAM O 901

Chain	Residue
O	ARG548
O	LEU549
O	TYR555
O	PHE556
O	LYS573
O	ASP598
O	PHE599
O	GLY600
O	ASP604
O	ASP620
O	PRO621
O	ASP622
O	ARG655
O	GLN678
O	PHE679
O	ALA680
O	TYR683

site_id	AC7
Number of Residues	5
Details	binding site for residue MG O 902

Chain	Residue
O	GLU37
O	GLU39
O	GLU192
O	GLU194
P	GDP1

Functional Information from PROSITE/UniProt

site_id	PS00466
Number of Residues	36
Details	ZF_TFIIS_1 Zinc finger TFIIS-type signature. CpnCksrnttpmmIQTRAaDEPplvrhaCrd...CkqhF

Chain	Residue	Details
S	CYS159-PHE194

site_id	PS01166
Number of Residues	13
Details	RNA_POL_BETA RNA polymerases beta chain signature. GdKFTTrTSQKGT

Chain	Residue	Details
B	GLY880-THR892

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	40
Details	ZN_FING: TFIIS-type => ECO:0000269\|PubMed:31835031, ECO:0007744\|PDB:6RIE

Chain	Residue	Details
S	TYR155-LYS195

site_id	SWS_FT_FI2
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269\|PubMed:31835031, ECO:0007744\|PDB:6RIE

Chain	Residue	Details
S	CYS159
S	CYS162
S	CYS187
S	CYS190

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING:

Chain	Residue	Details
O	LEU549
O	GLN678

site_id	SWS_FT_FI4
Number of Residues	4
Details	BINDING: BINDING => ECO:0000255\|PROSITE-ProRule:PRU00895

Chain	Residue	Details
O	SER569
O	LYS573
O	ASP598
O	ASP620

site_id	SWS_FT_FI5
Number of Residues	5
Details	SITE: Essential for RNA triphosphatase activity

Chain	Residue	Details
O	ARG77
O	LYS107
O	GLU126
O	ASP159
O	LYS161

site_id	SWS_FT_FI6
Number of Residues	5
Details	SITE: mRNA cap binding => ECO:0000255\|PROSITE-ProRule:PRU00895

Chain	Residue	Details
O	LYS607
O	ARG632
O	HIS682
O	GLU763
O	TYR836

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)