6QXS
Crystal structure of Enteroccocus faecalis thymidylate synthase (EfTS) in complex with FdUMP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006235 | biological_process | dTTP biosynthetic process |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0032259 | biological_process | methylation |
| B | 0004799 | molecular_function | thymidylate synthase activity |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006231 | biological_process | dTMP biosynthetic process |
| B | 0006235 | biological_process | dTTP biosynthetic process |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0009165 | biological_process | nucleotide biosynthetic process |
| B | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| B | 0032259 | biological_process | methylation |
| C | 0004799 | molecular_function | thymidylate synthase activity |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0006231 | biological_process | dTMP biosynthetic process |
| C | 0006235 | biological_process | dTTP biosynthetic process |
| C | 0008168 | molecular_function | methyltransferase activity |
| C | 0009165 | biological_process | nucleotide biosynthetic process |
| C | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| C | 0032259 | biological_process | methylation |
| D | 0004799 | molecular_function | thymidylate synthase activity |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0006231 | biological_process | dTMP biosynthetic process |
| D | 0006235 | biological_process | dTTP biosynthetic process |
| D | 0008168 | molecular_function | methyltransferase activity |
| D | 0009165 | biological_process | nucleotide biosynthetic process |
| D | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| D | 0032259 | biological_process | methylation |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 14 |
| Details | binding site for residue UFP B 401 |
| Chain | Residue |
| A | ARG177 |
| B | ASP220 |
| B | ASN228 |
| B | HIS258 |
| B | TYR260 |
| B | FFO403 |
| A | ARG178 |
| B | ARG22 |
| B | TYR145 |
| B | CYS197 |
| B | HIS198 |
| B | GLN216 |
| B | ARG217 |
| B | SER218 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 B 402 |
| Chain | Residue |
| A | ARG217 |
| A | SER218 |
| B | ARG177 |
| B | ARG178 |
| site_id | AC3 |
| Number of Residues | 16 |
| Details | binding site for residue FFO B 403 |
| Chain | Residue |
| B | ILE80 |
| B | TRP81 |
| B | TRP84 |
| B | LEU194 |
| B | ASP220 |
| B | LEU223 |
| B | GLY224 |
| B | PHE227 |
| B | TYR260 |
| B | ILE313 |
| B | ALA314 |
| B | UFP401 |
| B | HOH501 |
| B | HOH509 |
| B | HOH521 |
| B | HOH531 |
| site_id | AC4 |
| Number of Residues | 15 |
| Details | binding site for residue UFP D 401 |
| Chain | Residue |
| C | ARG177 |
| C | ARG178 |
| D | ARG22 |
| D | TYR145 |
| D | CYS197 |
| D | HIS198 |
| D | GLN216 |
| D | ARG217 |
| D | SER218 |
| D | ASP220 |
| D | ASN228 |
| D | HIS258 |
| D | TYR260 |
| D | FFO403 |
| D | HOH530 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 D 402 |
| Chain | Residue |
| C | ARG217 |
| C | SER218 |
| D | ARG177 |
| D | ARG178 |
| site_id | AC6 |
| Number of Residues | 15 |
| Details | binding site for residue FFO D 403 |
| Chain | Residue |
| D | PRO52 |
| D | ILE80 |
| D | TRP81 |
| D | TRP84 |
| D | LEU194 |
| D | ASP220 |
| D | LEU223 |
| D | GLY224 |
| D | ASN228 |
| D | ILE313 |
| D | ALA314 |
| D | UFP401 |
| D | HOH513 |
| D | HOH519 |
| D | HOH527 |
Functional Information from PROSITE/UniProt
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RrlIvsaWNpedvpsma.....LpPCHtmfQFyV |
| Chain | Residue | Details |
| A | ARG177-VAL205 | |
| B | ARG177-VAL205 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 3 |
| Details | ACT_SITE: Nucleophile => ECO:0000255|HAMAP-Rule:MF_00008 |
| Chain | Residue | Details |
| B | CYS197 | |
| C | CYS197 | |
| D | CYS197 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: in other chain => ECO:0000255|HAMAP-Rule:MF_00008 |
| Chain | Residue | Details |
| B | ARG22 | |
| D | ARG217 | |
| D | ASN228 | |
| D | HIS258 | |
| B | ARG217 | |
| B | ASN228 | |
| B | HIS258 | |
| C | ARG22 | |
| C | ARG217 | |
| C | ASN228 | |
| C | HIS258 | |
| D | ARG22 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 9 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_00008 |
| Chain | Residue | Details |
| B | ARG177 | |
| B | ASP220 | |
| B | ALA314 | |
| C | ARG177 | |
| C | ASP220 | |
| C | ALA314 | |
| D | ARG177 | |
| D | ASP220 | |
| D | ALA314 |
