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6PXV

Cryo-EM structure of full-length insulin receptor bound to 4 insulin. 3D refinement was focused on the extracellular region.

Functional Information from GO Data
ChainGOidnamespacecontents
A0001540molecular_functionamyloid-beta binding
A0001934biological_processpositive regulation of protein phosphorylation
A0002092biological_processpositive regulation of receptor internalization
A0003007biological_processheart morphogenesis
A0004672molecular_functionprotein kinase activity
A0004713molecular_functionprotein tyrosine kinase activity
A0004714molecular_functiontransmembrane receptor protein tyrosine kinase activity
A0005009molecular_functioninsulin receptor activity
A0005159molecular_functioninsulin-like growth factor receptor binding
A0005515molecular_functionprotein binding
A0005524molecular_functionATP binding
A0005525molecular_functionGTP binding
A0005635cellular_componentnuclear envelope
A0005764cellular_componentlysosome
A0005768cellular_componentendosome
A0005770cellular_componentlate endosome
A0005886cellular_componentplasma membrane
A0005899cellular_componentinsulin receptor complex
A0005901cellular_componentcaveola
A0006355biological_processregulation of DNA-templated transcription
A0006468biological_processprotein phosphorylation
A0006898biological_processreceptor-mediated endocytosis
A0007169biological_processcell surface receptor protein tyrosine kinase signaling pathway
A0007186biological_processG protein-coupled receptor signaling pathway
A0007612biological_processlearning
A0007613biological_processmemory
A0008284biological_processpositive regulation of cell population proliferation
A0008286biological_processinsulin receptor signaling pathway
A0008544biological_processepidermis development
A0008584biological_processmale gonad development
A0009887biological_processanimal organ morphogenesis
A0009897cellular_componentexternal side of plasma membrane
A0010008cellular_componentendosome membrane
A0016020cellular_componentmembrane
A0016310biological_processphosphorylation
A0018108biological_processpeptidyl-tyrosine phosphorylation
A0019904molecular_functionprotein domain specific binding
A0030238biological_processmale sex determination
A0030295molecular_functionprotein kinase activator activity
A0030325biological_processadrenal gland development
A0030335biological_processpositive regulation of cell migration
A0030424cellular_componentaxon
A0031017biological_processexocrine pancreas development
A0031623biological_processreceptor internalization
A0031981cellular_componentnuclear lumen
A0031994molecular_functioninsulin-like growth factor I binding
A0031995molecular_functioninsulin-like growth factor II binding
A0032148biological_processactivation of protein kinase B activity
A0032590cellular_componentdendrite membrane
A0032809cellular_componentneuronal cell body membrane
A0032869biological_processcellular response to insulin stimulus
A0038024molecular_functioncargo receptor activity
A0042593biological_processglucose homeostasis
A0043235cellular_componentreceptor complex
A0043243biological_processpositive regulation of protein-containing complex disassembly
A0043406biological_processpositive regulation of MAP kinase activity
A0043410biological_processpositive regulation of MAPK cascade
A0043548molecular_functionphosphatidylinositol 3-kinase binding
A0043559molecular_functioninsulin binding
A0043560molecular_functioninsulin receptor substrate binding
A0044877molecular_functionprotein-containing complex binding
A0045429biological_processpositive regulation of nitric oxide biosynthetic process
A0045725biological_processpositive regulation of glycogen biosynthetic process
A0045821biological_processpositive regulation of glycolytic process
A0045840biological_processpositive regulation of mitotic nuclear division
A0045893biological_processpositive regulation of DNA-templated transcription
A0045995biological_processregulation of embryonic development
A0046326biological_processpositive regulation of D-glucose import
A0046718biological_processsymbiont entry into host cell
A0046777biological_processprotein autophosphorylation
A0048639biological_processpositive regulation of developmental growth
A0051425molecular_functionPTB domain binding
A0051446biological_processpositive regulation of meiotic cell cycle
A0051897biological_processpositive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
A0060267biological_processpositive regulation of respiratory burst
A0070062cellular_componentextracellular exosome
A0071363biological_processcellular response to growth factor stimulus
A0097062biological_processdendritic spine maintenance
A0097242biological_processamyloid-beta clearance
A0150104biological_processtransport across blood-brain barrier
A1990535biological_processneuron projection maintenance
A2000194biological_processregulation of female gonad development
C0001540molecular_functionamyloid-beta binding
C0001934biological_processpositive regulation of protein phosphorylation
C0002092biological_processpositive regulation of receptor internalization
C0003007biological_processheart morphogenesis
C0004672molecular_functionprotein kinase activity
C0004713molecular_functionprotein tyrosine kinase activity
C0004714molecular_functiontransmembrane receptor protein tyrosine kinase activity
C0005009molecular_functioninsulin receptor activity
C0005159molecular_functioninsulin-like growth factor receptor binding
C0005515molecular_functionprotein binding
C0005524molecular_functionATP binding
C0005525molecular_functionGTP binding
C0005635cellular_componentnuclear envelope
C0005764cellular_componentlysosome
C0005768cellular_componentendosome
C0005770cellular_componentlate endosome
C0005886cellular_componentplasma membrane
C0005899cellular_componentinsulin receptor complex
C0005901cellular_componentcaveola
C0006355biological_processregulation of DNA-templated transcription
C0006468biological_processprotein phosphorylation
C0006898biological_processreceptor-mediated endocytosis
C0007169biological_processcell surface receptor protein tyrosine kinase signaling pathway
C0007186biological_processG protein-coupled receptor signaling pathway
C0007612biological_processlearning
C0007613biological_processmemory
C0008284biological_processpositive regulation of cell population proliferation
C0008286biological_processinsulin receptor signaling pathway
C0008544biological_processepidermis development
C0008584biological_processmale gonad development
C0009887biological_processanimal organ morphogenesis
C0009897cellular_componentexternal side of plasma membrane
C0010008cellular_componentendosome membrane
C0016020cellular_componentmembrane
C0016310biological_processphosphorylation
C0018108biological_processpeptidyl-tyrosine phosphorylation
C0019904molecular_functionprotein domain specific binding
C0030238biological_processmale sex determination
C0030295molecular_functionprotein kinase activator activity
C0030325biological_processadrenal gland development
C0030335biological_processpositive regulation of cell migration
C0030424cellular_componentaxon
C0031017biological_processexocrine pancreas development
C0031623biological_processreceptor internalization
C0031981cellular_componentnuclear lumen
C0031994molecular_functioninsulin-like growth factor I binding
C0031995molecular_functioninsulin-like growth factor II binding
C0032148biological_processactivation of protein kinase B activity
C0032590cellular_componentdendrite membrane
C0032809cellular_componentneuronal cell body membrane
C0032869biological_processcellular response to insulin stimulus
C0038024molecular_functioncargo receptor activity
C0042593biological_processglucose homeostasis
C0043235cellular_componentreceptor complex
C0043243biological_processpositive regulation of protein-containing complex disassembly
C0043406biological_processpositive regulation of MAP kinase activity
C0043410biological_processpositive regulation of MAPK cascade
C0043548molecular_functionphosphatidylinositol 3-kinase binding
C0043559molecular_functioninsulin binding
C0043560molecular_functioninsulin receptor substrate binding
C0044877molecular_functionprotein-containing complex binding
C0045429biological_processpositive regulation of nitric oxide biosynthetic process
C0045725biological_processpositive regulation of glycogen biosynthetic process
C0045821biological_processpositive regulation of glycolytic process
C0045840biological_processpositive regulation of mitotic nuclear division
C0045893biological_processpositive regulation of DNA-templated transcription
C0045995biological_processregulation of embryonic development
C0046326biological_processpositive regulation of D-glucose import
C0046718biological_processsymbiont entry into host cell
C0046777biological_processprotein autophosphorylation
C0048639biological_processpositive regulation of developmental growth
C0051425molecular_functionPTB domain binding
C0051446biological_processpositive regulation of meiotic cell cycle
C0051897biological_processpositive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
C0060267biological_processpositive regulation of respiratory burst
C0070062cellular_componentextracellular exosome
C0071363biological_processcellular response to growth factor stimulus
C0097062biological_processdendritic spine maintenance
C0097242biological_processamyloid-beta clearance
C0150104biological_processtransport across blood-brain barrier
C1990535biological_processneuron projection maintenance
C2000194biological_processregulation of female gonad development
D0005179molecular_functionhormone activity
D0005576cellular_componentextracellular region
D0006006biological_processglucose metabolic process
D0007165biological_processsignal transduction
D1901701biological_processcellular response to oxygen-containing compound
E0005179molecular_functionhormone activity
E0005576cellular_componentextracellular region
E0006006biological_processglucose metabolic process
E0007165biological_processsignal transduction
E1901701biological_processcellular response to oxygen-containing compound
F0005179molecular_functionhormone activity
F0005576cellular_componentextracellular region
F0006006biological_processglucose metabolic process
F0007165biological_processsignal transduction
F1901701biological_processcellular response to oxygen-containing compound
G0005179molecular_functionhormone activity
G0005576cellular_componentextracellular region
G0006006biological_processglucose metabolic process
G0007165biological_processsignal transduction
G1901701biological_processcellular response to oxygen-containing compound
Functional Information from PROSITE/UniProt
site_idPS00107
Number of Residues29
DetailsPROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGQGSFGMVYeGnardiikgeaetr.....VAVK
ChainResidueDetails
ALEU990-LYS1018

site_idPS00239
Number of Residues9
DetailsRECEPTOR_TYR_KIN_II Receptor tyrosine kinase class II signature. DIYetdYYR
ChainResidueDetails
AASP1144-ARG1152

site_idPS00262
Number of Residues15
DetailsINSULIN Insulin family signature. CCTSiCSlyqLenyC
ChainResidueDetails
DCYS61-CYS75

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues1846
DetailsTOPO_DOM: Extracellular => ECO:0000305
ChainResidueDetails
AHIS1-VAL731
APRO736-PHE929
CHIS1-VAL731
CPRO736-PHE929

site_idSWS_FT_FI2
Number of Residues44
DetailsTRANSMEM: Helical => ECO:0000255
ChainResidueDetails
ASER930-LEU952
CSER930-LEU952

site_idSWS_FT_FI3
Number of Residues2
DetailsACT_SITE: Proton donor/acceptor => ECO:0000269|PubMed:9312016
ChainResidueDetails
APHE1132
CPHE1132

site_idSWS_FT_FI4
Number of Residues10
DetailsBINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00159, ECO:0000269|PubMed:18278056
ChainResidueDetails
AILE1006
CTYR1150
AILE1030
AARG1077
AILE1136
ATYR1150
CILE1006
CILE1030
CARG1077
CILE1136

site_idSWS_FT_FI5
Number of Residues2
DetailsSITE: Insulin-binding => ECO:0000305
ChainResidueDetails
APHE39
CPHE39

site_idSWS_FT_FI6
Number of Residues4
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648
ChainResidueDetails
ASER373
ASER380
CSER373
CSER380

site_idSWS_FT_FI7
Number of Residues2
DetailsMOD_RES: Phosphotyrosine => ECO:0007744|PubMed:18669648
ChainResidueDetails
ATYR374
CTYR374

site_idSWS_FT_FI8
Number of Residues4
DetailsMOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000305
ChainResidueDetails
AASP965
AILE984
CASP965
CILE984

site_idSWS_FT_FI9
Number of Residues6
DetailsMOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000305|PubMed:3166375
ChainResidueDetails
ATYR972
AGLY1328
AALA1334
CTYR972
CGLY1328
CALA1334

site_idSWS_FT_FI10
Number of Residues2
DetailsMOD_RES: S-nitrosocysteine => ECO:0000269|PubMed:38056462
ChainResidueDetails
ALYS1056
CLYS1056

site_idSWS_FT_FI11
Number of Residues6
DetailsMOD_RES: Phosphotyrosine; by autocatalysis => ECO:0000269|PubMed:14690593, ECO:0000269|PubMed:16246733, ECO:0000269|PubMed:16271887, ECO:0000269|PubMed:18278056, ECO:0000269|PubMed:18767165, ECO:0000269|PubMed:3166375, ECO:0000269|PubMed:9312016
ChainResidueDetails
ALEU1158
AARG1162
ATRP1163
CLEU1158
CARG1162
CTRP1163

site_idSWS_FT_FI12
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16894147, ECO:0000269|PubMed:23302862, ECO:0000269|PubMed:2983222
ChainResidueDetails
AASN16
CASN16

site_idSWS_FT_FI13
Number of Residues6
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16894147, ECO:0000269|PubMed:23302862
ChainResidueDetails
AASN25
AASN111
AASN255
CASN25
CASN111
CASN255

site_idSWS_FT_FI14
Number of Residues14
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255
ChainResidueDetails
AASN78
CASN606
CASN624
CASN671
CGLU755
CTHR906
AASN295
AASN606
AASN624
AASN671
AGLU755
ATHR906
CASN78
CASN295

site_idSWS_FT_FI15
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16894147, ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:23302862
ChainResidueDetails
AASN215
CASN215

site_idSWS_FT_FI16
Number of Residues4
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16894147
ChainResidueDetails
AASN337
AASN397
CASN337
CASN397

site_idSWS_FT_FI17
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:16894147, ECO:0000269|PubMed:19349973
ChainResidueDetails
AASN418
CASN418

site_idSWS_FT_FI18
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:1472036, ECO:0000269|PubMed:19159218
ChainResidueDetails
AASN514
CASN514

site_idSWS_FT_FI19
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:2983222
ChainResidueDetails
APRO742
CPRO742

site_idSWS_FT_FI20
Number of Residues2
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:19349973
ChainResidueDetails
AGLY893
CGLY893

Catalytic Information from CSA
site_idMCSA1
Number of Residues4
DetailsM-CSA 246
ChainResidueDetails
APHE1132increase nucleophilicity, proton acceptor, proton donor, steric role
AILE1136electrostatic stabiliser, increase electrophilicity, promote heterolysis
AGLY1137metal ligand
ATYR1150metal ligand

site_idMCSA2
Number of Residues4
DetailsM-CSA 246
ChainResidueDetails
CPHE1132increase nucleophilicity, proton acceptor, proton donor, steric role
CILE1136electrostatic stabiliser, increase electrophilicity, promote heterolysis
CGLY1137metal ligand
CTYR1150metal ligand

226707

PDB entries from 2024-10-30

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