6PV8

Human alpha3beta4 nicotinic acetylcholine receptor in complex with AT-1001

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004888	molecular_function	transmembrane signaling receptor activity
A	0005216	molecular_function	monoatomic ion channel activity
A	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
A	0005506	molecular_function	iron ion binding
A	0006811	biological_process	monoatomic ion transport
A	0009055	molecular_function	electron transfer activity
A	0016020	cellular_component	membrane
A	0020037	molecular_function	heme binding
A	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
A	0022900	biological_process	electron transport chain
A	0034220	biological_process	monoatomic ion transmembrane transport
A	0042597	cellular_component	periplasmic space
A	0045211	cellular_component	postsynaptic membrane
B	0004888	molecular_function	transmembrane signaling receptor activity
B	0005216	molecular_function	monoatomic ion channel activity
B	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
B	0005506	molecular_function	iron ion binding
B	0006811	biological_process	monoatomic ion transport
B	0009055	molecular_function	electron transfer activity
B	0016020	cellular_component	membrane
B	0020037	molecular_function	heme binding
B	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
B	0022900	biological_process	electron transport chain
B	0034220	biological_process	monoatomic ion transmembrane transport
B	0042597	cellular_component	periplasmic space
B	0045211	cellular_component	postsynaptic membrane
C	0004888	molecular_function	transmembrane signaling receptor activity
C	0005216	molecular_function	monoatomic ion channel activity
C	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
C	0005506	molecular_function	iron ion binding
C	0006811	biological_process	monoatomic ion transport
C	0009055	molecular_function	electron transfer activity
C	0016020	cellular_component	membrane
C	0020037	molecular_function	heme binding
C	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
C	0022900	biological_process	electron transport chain
C	0034220	biological_process	monoatomic ion transmembrane transport
C	0042597	cellular_component	periplasmic space
C	0045211	cellular_component	postsynaptic membrane
D	0004888	molecular_function	transmembrane signaling receptor activity
D	0005216	molecular_function	monoatomic ion channel activity
D	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
D	0005506	molecular_function	iron ion binding
D	0006811	biological_process	monoatomic ion transport
D	0009055	molecular_function	electron transfer activity
D	0016020	cellular_component	membrane
D	0020037	molecular_function	heme binding
D	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
D	0022900	biological_process	electron transport chain
D	0034220	biological_process	monoatomic ion transmembrane transport
D	0042597	cellular_component	periplasmic space
D	0045211	cellular_component	postsynaptic membrane
E	0004888	molecular_function	transmembrane signaling receptor activity
E	0005216	molecular_function	monoatomic ion channel activity
E	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
E	0005506	molecular_function	iron ion binding
E	0006811	biological_process	monoatomic ion transport
E	0009055	molecular_function	electron transfer activity
E	0016020	cellular_component	membrane
E	0020037	molecular_function	heme binding
E	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
E	0022900	biological_process	electron transport chain
E	0034220	biological_process	monoatomic ion transmembrane transport
E	0042597	cellular_component	periplasmic space
E	0045211	cellular_component	postsynaptic membrane

Functional Information from PROSITE/UniProt

site_id	PS00236
Number of Residues	15
Details	NEUROTR_ION_CHANNEL Neurotransmitter-gated ion-channels signature. CkIeVkyFPfDqqnC

Chain	Residue	Details
B	CYS132-CYS146
A	CYS128-CYS142

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site_id	PS00290
Number of Residues	7
Details	IG_MHC Immunoglobulins and major histocompatibility complex proteins signature. YTCEATH

Chain	Residue	Details
G	TYR213-HIS219

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	762
Details	TOPO_DOM: Extracellular => ECO:0000255

Chain	Residue	Details
B	ARG1-LEU218
B	LEU257-GLY275
B	LEU458-ASP477
C	ARG1-LEU218
C	LEU257-GLY275
C	LEU458-ASP477
E	ARG1-LEU218
E	LEU257-GLY275
E	LEU458-ASP477

---

site_id	SWS_FT_FI2
Number of Residues	231
Details	TRANSMEM: Helical => ECO:0000269|PubMed:31488329, ECO:0007744|PDB:6PV7, ECO:0007744|PDB:6PV8

Chain	Residue	Details
B	ILE219-TYR234
E	GLU241-PHE256
E	LYS276-HIS300
E	ALA434-PHE457
B	GLU241-PHE256
B	LYS276-HIS300
B	ALA434-PHE457
C	ILE219-TYR234
C	GLU241-PHE256
C	LYS276-HIS300
C	ALA434-PHE457
E	ILE219-TYR234

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site_id	SWS_FT_FI3
Number of Residues	15
Details	TOPO_DOM: Cytoplasmic => ECO:0000255

Chain	Residue	Details
B	LEU235-GLY240
C	LEU235-GLY240
E	LEU235-GLY240

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site_id	SWS_FT_FI4
Number of Residues	3
Details	BINDING: BINDING => ECO:0000269|PubMed:31488329, ECO:0007744|PDB:6PV8

Chain	Residue	Details
B	GLU241
C	GLU241
E	GLU241
D	ALA331

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site_id	SWS_FT_FI5
Number of Residues	3
Details	SITE: Key residue that facilitates effective access of the conotoxin BuIA to the channel binding site => ECO:0000250|UniProtKB:P12392

Chain	Residue	Details
B	LYS61
C	LYS61
E	LYS61
D	ASN141

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site_id	SWS_FT_FI6
Number of Residues	3
Details	SITE: Key residue for a low dissociation (K(off)) from the conotoxin BuIA => ECO:0000250|UniProtKB:P12392

Chain	Residue	Details
B	ILE113
C	ILE113
E	ILE113

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site_id	SWS_FT_FI7
Number of Residues	12
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255

Chain	Residue	Details
B	ASN15
E	ASN72
E	ASN117
E	ASN145
B	ASN72
B	ASN117
B	ASN145
C	ASN15
C	ASN72
C	ASN117
C	ASN145
E	ASN15

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