Functional Information from GO Data
| Chain | GOid | namespace | contents |
| X | 0004146 | molecular_function | dihydrofolate reductase activity |
| X | 0005829 | cellular_component | cytosol |
| X | 0006730 | biological_process | one-carbon metabolic process |
| X | 0016491 | molecular_function | oxidoreductase activity |
| X | 0046452 | biological_process | dihydrofolate metabolic process |
| X | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| X | 0046655 | biological_process | folic acid metabolic process |
| X | 0050661 | molecular_function | NADP binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 16 |
| Details | binding site for residue MTX X 201 |
| Chain | Residue |
| X | LEU5 |
| X | PHE92 |
| X | THR111 |
| X | NAP202 |
| X | HOH311 |
| X | HOH324 |
| X | HOH329 |
| X | HOH346 |
| X | VAL6 |
| X | ALA7 |
| X | LEU20 |
| X | ASP27 |
| X | LEU28 |
| X | VAL31 |
| X | LYS32 |
| X | ARG57 |
| site_id | AC2 |
| Number of Residues | 29 |
| Details | binding site for residue NAP X 202 |
| Chain | Residue |
| X | VAL6 |
| X | ALA7 |
| X | ILE14 |
| X | GLY15 |
| X | ASN18 |
| X | GLN19 |
| X | LEU20 |
| X | TRP22 |
| X | GLY43 |
| X | ARG44 |
| X | LYS45 |
| X | THR46 |
| X | LEU62 |
| X | THR63 |
| X | SER64 |
| X | ILE79 |
| X | PHE92 |
| X | GLY94 |
| X | GLN95 |
| X | THR96 |
| X | GLU100 |
| X | THR121 |
| X | MTX201 |
| X | HOH308 |
| X | HOH314 |
| X | HOH323 |
| X | HOH325 |
| X | HOH329 |
| X | HOH338 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | binding site for residue GOL X 203 |
| Chain | Residue |
| X | ARG12 |
| X | PRO125 |
| X | TYR126 |
| X | GLU132 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGfenqLPWhlpn.DlkhVkklS |
| Chain | Residue | Details |
| X | VAL13-SER35 | |
Functional Information from SwissProt/UniProt
| Chain | Residue | Details |
| X | LEU5 | |
| X | ASP27 | |
| X | SER49 | |
| X | ARG57 | |
| X | PHE92 | |
| Chain | Residue | Details |
| X | VAL6 | |
| X | ILE14 | |
| X | GLY43 | |
| X | LEU62 | |
| X | GLU100 | |
| X | THR121 | |
