6OFB
Crystal structure of human glutamine-dependent NAD+ synthetase complexed with NaAD+, AMP, pyrophosphate, and Mg2+
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0003952 | molecular_function | NAD+ synthase (glutamine-hydrolyzing) activity |
| A | 0004359 | molecular_function | glutaminase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0009435 | biological_process | NAD+ biosynthetic process |
| A | 0016874 | molecular_function | ligase activity |
| A | 0034354 | biological_process | 'de novo' NAD+ biosynthetic process from L-tryptophan |
| A | 0034355 | biological_process | NAD+ biosynthetic process via the salvage pathway |
| B | 0000166 | molecular_function | nucleotide binding |
| B | 0003952 | molecular_function | NAD+ synthase (glutamine-hydrolyzing) activity |
| B | 0004359 | molecular_function | glutaminase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0009435 | biological_process | NAD+ biosynthetic process |
| B | 0016874 | molecular_function | ligase activity |
| B | 0034354 | biological_process | 'de novo' NAD+ biosynthetic process from L-tryptophan |
| B | 0034355 | biological_process | NAD+ biosynthetic process via the salvage pathway |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 13 |
| Details | binding site for residue DND A 901 |
| Chain | Residue |
| A | ASN483 |
| B | GLN349 |
| B | GLN498 |
| B | LEU510 |
| B | ASP535 |
| A | ARG487 |
| A | LEU526 |
| A | THR527 |
| A | LYS528 |
| A | ASP590 |
| A | HIS648 |
| A | LYS649 |
| A | AMP902 |
| site_id | AC2 |
| Number of Residues | 10 |
| Details | binding site for residue AMP A 902 |
| Chain | Residue |
| A | PRO355 |
| A | LEU356 |
| A | SER357 |
| A | CYS416 |
| A | MET418 |
| A | ARG489 |
| A | CYS531 |
| A | ALA573 |
| A | DND901 |
| A | POP903 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | binding site for residue POP A 903 |
| Chain | Residue |
| A | SER357 |
| A | GLY359 |
| A | ASP361 |
| A | SER362 |
| A | AMP902 |
| A | MG904 |
| A | CL909 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue MG A 904 |
| Chain | Residue |
| A | GLY359 |
| A | VAL360 |
| A | ASP361 |
| A | LYS544 |
| A | POP903 |
| A | CL909 |
| site_id | AC5 |
| Number of Residues | 1 |
| Details | binding site for residue CL A 905 |
| Chain | Residue |
| A | ARG145 |
| site_id | AC6 |
| Number of Residues | 1 |
| Details | binding site for residue CL A 906 |
| Chain | Residue |
| B | ARG93 |
| site_id | AC7 |
| Number of Residues | 1 |
| Details | binding site for residue CL A 907 |
| Chain | Residue |
| A | ARG476 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue CL A 908 |
| Chain | Residue |
| A | VAL360 |
| A | LYS544 |
| A | ARG548 |
| A | ALA571 |
| A | CL909 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | binding site for residue CL A 909 |
| Chain | Residue |
| A | VAL360 |
| A | ALA571 |
| A | POP903 |
| A | MG904 |
| A | CL908 |
| site_id | AD1 |
| Number of Residues | 19 |
| Details | binding site for residue DND B 901 |
| Chain | Residue |
| A | GLN349 |
| A | GLN498 |
| A | LEU510 |
| A | LEU511 |
| A | ASP535 |
| B | LEU479 |
| B | GLN482 |
| B | ASN483 |
| B | ARG487 |
| B | LEU526 |
| B | THR527 |
| B | LYS528 |
| B | ASP530 |
| B | LEU575 |
| B | ASP590 |
| B | HIS648 |
| B | LYS649 |
| B | PHE674 |
| B | AMP902 |
| site_id | AD2 |
| Number of Residues | 10 |
| Details | binding site for residue AMP B 902 |
| Chain | Residue |
| B | PRO355 |
| B | LEU356 |
| B | SER357 |
| B | CYS416 |
| B | MET418 |
| B | ARG489 |
| B | ALA573 |
| B | LEU575 |
| B | DND901 |
| B | POP903 |
| site_id | AD3 |
| Number of Residues | 8 |
| Details | binding site for residue POP B 903 |
| Chain | Residue |
| B | SER357 |
| B | GLY359 |
| B | ASP361 |
| B | SER362 |
| B | LYS544 |
| B | AMP902 |
| B | MG904 |
| B | CL908 |
| site_id | AD4 |
| Number of Residues | 6 |
| Details | binding site for residue MG B 904 |
| Chain | Residue |
| B | GLY359 |
| B | VAL360 |
| B | ASP361 |
| B | LYS544 |
| B | POP903 |
| B | CL908 |
| site_id | AD5 |
| Number of Residues | 1 |
| Details | binding site for residue CL B 905 |
| Chain | Residue |
| B | ARG476 |
| site_id | AD6 |
| Number of Residues | 2 |
| Details | binding site for residue CL B 906 |
| Chain | Residue |
| A | SER475 |
| B | SER475 |
| site_id | AD7 |
| Number of Residues | 3 |
| Details | binding site for residue CL B 907 |
| Chain | Residue |
| B | ARG548 |
| B | ALA571 |
| B | CL908 |
| site_id | AD8 |
| Number of Residues | 5 |
| Details | binding site for residue CL B 908 |
| Chain | Residue |
| B | ALA571 |
| B | POP903 |
| B | MG904 |
| B | CL907 |
| B | VAL360 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 540 |
| Details | Domain: {"description":"CN hydrolase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00054","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Proton acceptor; for glutaminase activity","evidences":[{"source":"UniProtKB","id":"P9WJJ3","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Active site: {"description":"For glutaminase activity","evidences":[{"source":"UniProtKB","id":"P9WJJ3","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Nucleophile; for glutaminase activity","evidences":[{"source":"UniProtKB","id":"P9WJJ3","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Active site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 14 |
| Details | Binding site: {"evidences":[{"evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
