6OB3

Crystal structure of G13D-KRAS (GMPPNP-bound) in complex with GAP-related domain (GRD) of neurofibromin (NF1)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003924	molecular_function	GTPase activity
A	0005525	molecular_function	GTP binding
A	0007165	biological_process	signal transduction
A	0016020	cellular_component	membrane
B	0043087	biological_process	regulation of GTPase activity
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
C	0007165	biological_process	signal transduction
C	0016020	cellular_component	membrane
D	0043087	biological_process	regulation of GTPase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	28
Details	binding site for residue GNP A 201

Chain	Residue
A	GLY12
A	ASP30
A	PRO34
A	THR35
A	GLY60
A	GLN61
A	ASN116
A	LYS117
A	ASP119
A	SER145
A	ALA146
A	ASP13
A	LYS147
A	MG202
A	HOH323
A	HOH328
A	HOH329
A	HOH332
A	HOH346
A	HOH364
B	ARG1276
A	VAL14
A	GLY15
A	LYS16
A	SER17
A	ALA18
A	PHE28
A	VAL29

---

site_id	AC2
Number of Residues	5
Details	binding site for residue MG A 202

Chain	Residue
A	SER17
A	THR35
A	GNP201
A	HOH328
A	HOH332

---

site_id	AC3
Number of Residues	4
Details	binding site for residue CL B 1501

Chain	Residue
B	PHE1376
B	PRO1377
B	GLN1378
B	ASN1379

---

site_id	AC4
Number of Residues	1
Details	binding site for residue CL B 1502

Chain	Residue
B	HOH1691

---

site_id	AC5
Number of Residues	2
Details	binding site for residue MES B 1503

Chain	Residue
B	ASP1248
B	HOH1654

---

site_id	AC6
Number of Residues	2
Details	binding site for residue MES B 1504

Chain	Residue
B	TRP1258
D	GLU1333

---

site_id	AC7
Number of Residues	5
Details	binding site for residue MES B 1505

Chain	Residue
B	GLN1336
B	ARG1337
B	LEU1340
B	HOH1604
D	LYS1408

---

site_id	AC8
Number of Residues	28
Details	binding site for residue GNP C 201

Chain	Residue
C	GLY12
C	ASP13
C	VAL14
C	GLY15
C	LYS16
C	SER17
C	ALA18
C	PHE28
C	VAL29
C	ASP30
C	PRO34
C	THR35
C	GLY60
C	ASN116
C	LYS117
C	ASP119
C	LEU120
C	SER145
C	ALA146
C	LYS147
C	MG202
C	HOH306
C	HOH310
C	HOH311
C	HOH313
C	HOH314
C	HOH315
C	HOH327

---

site_id	AC9
Number of Residues	5
Details	binding site for residue MG C 202

Chain	Residue
C	SER17
C	THR35
C	GNP201
C	HOH306
C	HOH314

---

site_id	AD1
Number of Residues	1
Details	binding site for residue CL C 203

Chain	Residue
C	ARG68

---

site_id	AD2
Number of Residues	7
Details	binding site for residue MES D 1501

Chain	Residue
A	GLN22
A	PHE28
A	LYS147
A	THR148
D	ASP1248
D	HIS1251
D	HOH1639

---

site_id	AD3
Number of Residues	4
Details	binding site for residue MES D 1502

Chain	Residue
D	LEU1340
D	PRO1442
D	ASP1445
D	HOH1609

---

Functional Information from PROSITE/UniProt

site_id	PS00509
Number of Residues	15
Details	RAS_GTPASE_ACTIV_1 Ras GTPase-activating proteins domain signature. SaMFLRFINPAIVSP

Chain	Residue	Details
B	SER1386-PRO1400

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	BINDING: BINDING => ECO:0000269|PubMed:22431598, ECO:0000269|PubMed:22566140

Chain	Residue	Details
A	GLY10
A	VAL29
A	ALA59
A	ASN116
C	GLY10
C	VAL29
C	ALA59
C	ASN116

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: N-acetylmethionine; in GTPase KRas; alternate => ECO:0000269|Ref.17

Chain	Residue	Details
A	MET1
C	MET1

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N-acetylthreonine; in GTPase KRas, N-terminally processed => ECO:0000269|Ref.17

Chain	Residue	Details
A	THR2
C	THR2

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:22711838

Chain	Residue	Details
A	LYS104
C	LYS104

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	CARBOHYD: (Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL => ECO:0000269|PubMed:19744486

Chain	Residue	Details
A	THR35
C	THR35

---