6NT2

type 1 PRMT in complex with the inhibitor GSK3368715

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001701	biological_process	in utero embryonic development
A	0003723	molecular_function	RNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005764	cellular_component	lysosome
A	0005765	cellular_component	lysosomal membrane
A	0005829	cellular_component	cytosol
A	0006338	biological_process	chromatin remodeling
A	0006479	biological_process	protein methylation
A	0006974	biological_process	DNA damage response
A	0007166	biological_process	cell surface receptor signaling pathway
A	0008168	molecular_function	methyltransferase activity
A	0008170	molecular_function	N-methyltransferase activity
A	0008276	molecular_function	protein methyltransferase activity
A	0008284	biological_process	positive regulation of cell population proliferation
A	0008327	molecular_function	methyl-CpG binding
A	0008380	biological_process	RNA splicing
A	0016020	cellular_component	membrane
A	0016274	molecular_function	protein-arginine N-methyltransferase activity
A	0018216	biological_process	peptidyl-arginine methylation
A	0019082	biological_process	viral protein processing
A	0019899	molecular_function	enzyme binding
A	0030510	biological_process	regulation of BMP signaling pathway
A	0031175	biological_process	neuron projection development
A	0032259	biological_process	methylation
A	0034709	cellular_component	methylosome
A	0035241	molecular_function	protein-arginine omega-N monomethyltransferase activity
A	0035242	molecular_function	protein-arginine omega-N asymmetric methyltransferase activity
A	0042054	molecular_function	histone methyltransferase activity
A	0042802	molecular_function	identical protein binding
A	0044020	molecular_function	histone H4R3 methyltransferase activity
A	0045648	biological_process	positive regulation of erythrocyte differentiation
A	0045652	biological_process	regulation of megakaryocyte differentiation
A	0045653	biological_process	negative regulation of megakaryocyte differentiation
A	0045727	biological_process	positive regulation of translation
A	0046329	biological_process	negative regulation of JNK cascade
A	0046985	biological_process	positive regulation of hemoglobin biosynthetic process
A	0048273	molecular_function	mitogen-activated protein kinase p38 binding
A	0048738	biological_process	cardiac muscle tissue development
A	0051260	biological_process	protein homooligomerization
A	0061431	biological_process	cellular response to methionine
A	0106080	molecular_function	GATOR1 complex binding
A	1900745	biological_process	positive regulation of p38MAPK cascade
A	1904047	molecular_function	S-adenosyl-L-methionine binding
A	1904263	biological_process	positive regulation of TORC1 signaling
A	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination
B	0001701	biological_process	in utero embryonic development
B	0003723	molecular_function	RNA binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005764	cellular_component	lysosome
B	0005765	cellular_component	lysosomal membrane
B	0005829	cellular_component	cytosol
B	0006338	biological_process	chromatin remodeling
B	0006479	biological_process	protein methylation
B	0006974	biological_process	DNA damage response
B	0007166	biological_process	cell surface receptor signaling pathway
B	0008168	molecular_function	methyltransferase activity
B	0008170	molecular_function	N-methyltransferase activity
B	0008276	molecular_function	protein methyltransferase activity
B	0008284	biological_process	positive regulation of cell population proliferation
B	0008327	molecular_function	methyl-CpG binding
B	0008380	biological_process	RNA splicing
B	0016020	cellular_component	membrane
B	0016274	molecular_function	protein-arginine N-methyltransferase activity
B	0018216	biological_process	peptidyl-arginine methylation
B	0019082	biological_process	viral protein processing
B	0019899	molecular_function	enzyme binding
B	0030510	biological_process	regulation of BMP signaling pathway
B	0031175	biological_process	neuron projection development
B	0032259	biological_process	methylation
B	0034709	cellular_component	methylosome
B	0035241	molecular_function	protein-arginine omega-N monomethyltransferase activity
B	0035242	molecular_function	protein-arginine omega-N asymmetric methyltransferase activity
B	0042054	molecular_function	histone methyltransferase activity
B	0042802	molecular_function	identical protein binding
B	0044020	molecular_function	histone H4R3 methyltransferase activity
B	0045648	biological_process	positive regulation of erythrocyte differentiation
B	0045652	biological_process	regulation of megakaryocyte differentiation
B	0045653	biological_process	negative regulation of megakaryocyte differentiation
B	0045727	biological_process	positive regulation of translation
B	0046329	biological_process	negative regulation of JNK cascade
B	0046985	biological_process	positive regulation of hemoglobin biosynthetic process
B	0048273	molecular_function	mitogen-activated protein kinase p38 binding
B	0048738	biological_process	cardiac muscle tissue development
B	0051260	biological_process	protein homooligomerization
B	0061431	biological_process	cellular response to methionine
B	0106080	molecular_function	GATOR1 complex binding
B	1900745	biological_process	positive regulation of p38MAPK cascade
B	1904047	molecular_function	S-adenosyl-L-methionine binding
B	1904263	biological_process	positive regulation of TORC1 signaling
B	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination
C	0001701	biological_process	in utero embryonic development
C	0003723	molecular_function	RNA binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005764	cellular_component	lysosome
C	0005765	cellular_component	lysosomal membrane
C	0005829	cellular_component	cytosol
C	0006338	biological_process	chromatin remodeling
C	0006479	biological_process	protein methylation
C	0006974	biological_process	DNA damage response
C	0007166	biological_process	cell surface receptor signaling pathway
C	0008168	molecular_function	methyltransferase activity
C	0008170	molecular_function	N-methyltransferase activity
C	0008276	molecular_function	protein methyltransferase activity
C	0008284	biological_process	positive regulation of cell population proliferation
C	0008327	molecular_function	methyl-CpG binding
C	0008380	biological_process	RNA splicing
C	0016020	cellular_component	membrane
C	0016274	molecular_function	protein-arginine N-methyltransferase activity
C	0018216	biological_process	peptidyl-arginine methylation
C	0019082	biological_process	viral protein processing
C	0019899	molecular_function	enzyme binding
C	0030510	biological_process	regulation of BMP signaling pathway
C	0031175	biological_process	neuron projection development
C	0032259	biological_process	methylation
C	0034709	cellular_component	methylosome
C	0035241	molecular_function	protein-arginine omega-N monomethyltransferase activity
C	0035242	molecular_function	protein-arginine omega-N asymmetric methyltransferase activity
C	0042054	molecular_function	histone methyltransferase activity
C	0042802	molecular_function	identical protein binding
C	0044020	molecular_function	histone H4R3 methyltransferase activity
C	0045648	biological_process	positive regulation of erythrocyte differentiation
C	0045652	biological_process	regulation of megakaryocyte differentiation
C	0045653	biological_process	negative regulation of megakaryocyte differentiation
C	0045727	biological_process	positive regulation of translation
C	0046329	biological_process	negative regulation of JNK cascade
C	0046985	biological_process	positive regulation of hemoglobin biosynthetic process
C	0048273	molecular_function	mitogen-activated protein kinase p38 binding
C	0048738	biological_process	cardiac muscle tissue development
C	0051260	biological_process	protein homooligomerization
C	0061431	biological_process	cellular response to methionine
C	0106080	molecular_function	GATOR1 complex binding
C	1900745	biological_process	positive regulation of p38MAPK cascade
C	1904047	molecular_function	S-adenosyl-L-methionine binding
C	1904263	biological_process	positive regulation of TORC1 signaling
C	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination
D	0001701	biological_process	in utero embryonic development
D	0003723	molecular_function	RNA binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005764	cellular_component	lysosome
D	0005765	cellular_component	lysosomal membrane
D	0005829	cellular_component	cytosol
D	0006338	biological_process	chromatin remodeling
D	0006479	biological_process	protein methylation
D	0006974	biological_process	DNA damage response
D	0007166	biological_process	cell surface receptor signaling pathway
D	0008168	molecular_function	methyltransferase activity
D	0008170	molecular_function	N-methyltransferase activity
D	0008276	molecular_function	protein methyltransferase activity
D	0008284	biological_process	positive regulation of cell population proliferation
D	0008327	molecular_function	methyl-CpG binding
D	0008380	biological_process	RNA splicing
D	0016020	cellular_component	membrane
D	0016274	molecular_function	protein-arginine N-methyltransferase activity
D	0018216	biological_process	peptidyl-arginine methylation
D	0019082	biological_process	viral protein processing
D	0019899	molecular_function	enzyme binding
D	0030510	biological_process	regulation of BMP signaling pathway
D	0031175	biological_process	neuron projection development
D	0032259	biological_process	methylation
D	0034709	cellular_component	methylosome
D	0035241	molecular_function	protein-arginine omega-N monomethyltransferase activity
D	0035242	molecular_function	protein-arginine omega-N asymmetric methyltransferase activity
D	0042054	molecular_function	histone methyltransferase activity
D	0042802	molecular_function	identical protein binding
D	0044020	molecular_function	histone H4R3 methyltransferase activity
D	0045648	biological_process	positive regulation of erythrocyte differentiation
D	0045652	biological_process	regulation of megakaryocyte differentiation
D	0045653	biological_process	negative regulation of megakaryocyte differentiation
D	0045727	biological_process	positive regulation of translation
D	0046329	biological_process	negative regulation of JNK cascade
D	0046985	biological_process	positive regulation of hemoglobin biosynthetic process
D	0048273	molecular_function	mitogen-activated protein kinase p38 binding
D	0048738	biological_process	cardiac muscle tissue development
D	0051260	biological_process	protein homooligomerization
D	0061431	biological_process	cellular response to methionine
D	0106080	molecular_function	GATOR1 complex binding
D	1900745	biological_process	positive regulation of p38MAPK cascade
D	1904047	molecular_function	S-adenosyl-L-methionine binding
D	1904263	biological_process	positive regulation of TORC1 signaling
D	1905168	biological_process	positive regulation of double-strand break repair via homologous recombination

Functional Information from PDB Data

site_id	AC1
Number of Residues	22
Details	binding site for residue SAH A 401

Chain	Residue
A	TYR53
A	LEU102
A	GLU118
A	CYS119
A	GLY144
A	LYS145
A	VAL146
A	GLU147
A	MET173
A	THR176
A	HOH504
A	PHE54
A	HOH515
A	HOH563
A	HOH583
A	TYR57
A	HIS63
A	MET66
A	ARG72
A	GLY96
A	SER97
A	ILE101

---

site_id	AC2
Number of Residues	11
Details	binding site for residue KZS A 402

Chain	Residue
A	TYR57
A	GLU65
A	GLU162
A	TRP163
A	MET164
A	TYR166
A	GLU171
A	HIS311
A	HOH538
A	HOH552
D	TYR53

---

site_id	AC3
Number of Residues	4
Details	binding site for residue GOL A 403

Chain	Residue
A	TRP215
A	ASN218
A	TYR220
A	HOH543

---

site_id	AC4
Number of Residues	7
Details	binding site for residue GOL A 404

Chain	Residue
A	PHE169
A	SER172
A	ASN175
A	LEU265
A	LEU347
A	HOH584
A	HOH598

---

site_id	AC5
Number of Residues	6
Details	binding site for residue GOL A 405

Chain	Residue
A	TYR166
A	VAL237
A	LYS313
A	GOL408
A	HOH513
A	HOH549

---

site_id	AC6
Number of Residues	5
Details	binding site for residue GOL A 406

Chain	Residue
A	ASP205
A	TYR208
A	ASP238
A	VAL239
A	GLN244

---

site_id	AC7
Number of Residues	5
Details	binding site for residue GOL A 407

Chain	Residue
A	GLU204
A	ASP205
A	ARG206
A	GLN207
A	TYR208

---

site_id	AC8
Number of Residues	7
Details	binding site for residue GOL A 408

Chain	Residue
A	PRO235
A	VAL237
A	MET370
A	ARG371
A	GOL405
A	HOH508
A	HOH538

---

site_id	AC9
Number of Residues	6
Details	binding site for residue GOL A 409

Chain	Residue
A	ASN289
A	ILE290
A	GLU291
A	ARG298
A	GLY300
A	SER302

---

site_id	AD1
Number of Residues	23
Details	binding site for residue SAH D 401

Chain	Residue
D	TYR53
D	PHE54
D	TYR57
D	HIS63
D	MET66
D	ARG72
D	GLY96
D	SER97
D	ILE101
D	LEU102
D	GLU118
D	CYS119
D	GLY144
D	LYS145
D	VAL146
D	GLU147
D	GLU162
D	MET173
D	THR176
D	HOH501
D	HOH502
D	HOH533
D	HOH563

---

site_id	AD2
Number of Residues	17
Details	binding site for residue KZS D 402

Chain	Residue
D	SER56
D	TYR57
D	ILE62
D	GLU65
D	GLU162
D	TRP163
D	MET164
D	TYR166
D	TYR170
D	GLU171
D	HIS311
D	HOH518
D	HOH535
D	HOH543
A	SER49
A	TYR53
D	TYR53

---

site_id	AD3
Number of Residues	9
Details	binding site for residue GOL D 403

Chain	Residue
A	LEU67
A	LYS68
A	GLU70
A	HOH539
D	TRP215
D	ASN218
D	VAL219
D	TYR220
D	HOH541

---

site_id	AD4
Number of Residues	8
Details	binding site for residue GOL D 404

Chain	Residue
D	PHE169
D	SER172
D	ASN175
D	LEU265
D	PRO339
D	LEU347
D	HOH569
D	HOH587

---

site_id	AD5
Number of Residues	4
Details	binding site for residue GOL D 405

Chain	Residue
D	TYR166
D	LYS313
D	GOL408
D	HOH507

---

site_id	AD6
Number of Residues	6
Details	binding site for residue GOL D 406

Chain	Residue
D	ASP205
D	TYR208
D	VAL239
D	VAL240
D	ASP241
D	GLN244

---

site_id	AD7
Number of Residues	4
Details	binding site for residue GOL D 407

Chain	Residue
D	GLU204
D	ARG206
D	GLN207
D	HOH566

---

site_id	AD8
Number of Residues	6
Details	binding site for residue GOL D 408

Chain	Residue
D	TYR170
D	PRO235
D	ARG371
D	GOL405
D	HOH518
D	HOH520

---

site_id	AD9
Number of Residues	23
Details	binding site for residue SAH B 401

Chain	Residue
B	TYR53
B	PHE54
B	TYR57
B	HIS63
B	MET66
B	ARG72
B	GLY96
B	SER97
B	ILE101
B	LEU102
B	GLU118
B	CYS119
B	GLY144
B	LYS145
B	VAL146
B	GLU147
B	GLU162
B	MET173
B	THR176
B	HOH503
B	HOH542
B	HOH545
B	HOH575

---

site_id	AE1
Number of Residues	14
Details	binding site for residue KZS B 402

Chain	Residue
B	SER56
B	TYR57
B	ILE62
B	GLU65
B	GLU162
B	TRP163
B	MET164
B	TYR166
B	TYR170
B	GLU171
B	HIS311
B	HOH538
B	HOH561
C	SER49

---

site_id	AE2
Number of Residues	4
Details	binding site for residue GOL B 403

Chain	Residue
B	TRP215
B	ASN218
B	TYR220
B	HOH584

---

site_id	AE3
Number of Residues	7
Details	binding site for residue GOL B 404

Chain	Residue
B	PHE169
B	SER172
B	ASN175
B	LEU265
B	PRO339
B	HOH591
B	HOH608

---

site_id	AE4
Number of Residues	6
Details	binding site for residue GOL B 405

Chain	Residue
B	TYR166
B	VAL237
B	LYS313
B	GOL408
B	HOH505
B	HOH582

---

site_id	AE5
Number of Residues	7
Details	binding site for residue GOL B 406

Chain	Residue
B	ASP205
B	TYR208
B	ASP238
B	VAL239
B	GLN244
B	HOH553
B	HOH588

---

site_id	AE6
Number of Residues	6
Details	binding site for residue GOL B 407

Chain	Residue
B	GLU204
B	ASP205
B	ARG206
B	GLN207
B	TYR208
B	GLN244

---

site_id	AE7
Number of Residues	5
Details	binding site for residue GOL B 408

Chain	Residue
B	VAL237
B	ARG371
B	GOL405
B	HOH538
B	HOH630

---

site_id	AE8
Number of Residues	5
Details	binding site for residue DTT B 409

Chain	Residue
B	PHE81
B	HIS82
B	HIS296
B	HOH593
B	HOH614

---

site_id	AE9
Number of Residues	21
Details	binding site for residue SAH C 401

Chain	Residue
C	TYR53
C	TYR57
C	HIS63
C	MET66
C	ARG72
C	GLY96
C	SER97
C	ILE101
C	LEU102
C	GLU118
C	CYS119
C	GLY144
C	LYS145
C	VAL146
C	GLU147
C	MET173
C	THR176
C	HOH501
C	HOH504
C	HOH563
C	HOH564

---

site_id	AF1
Number of Residues	13
Details	binding site for residue KZS C 402

Chain	Residue
B	SER49
C	SER56
C	TYR57
C	ILE62
C	GLU65
C	GLU162
C	TRP163
C	MET164
C	TYR166
C	TYR170
C	GLU171
C	HIS311
C	HOH544

---

site_id	AF2
Number of Residues	5
Details	binding site for residue GOL C 403

Chain	Residue
B	GLU70
B	HOH507
C	TRP215
C	ASN218
C	TYR220

---

site_id	AF3
Number of Residues	8
Details	binding site for residue GOL C 404

Chain	Residue
C	PHE169
C	SER172
C	ASN175
C	LEU265
C	PRO339
C	LEU347
C	HOH539
C	HOH575

---

site_id	AF4
Number of Residues	5
Details	binding site for residue GOL C 405

Chain	Residue
C	ASP205
C	TYR208
C	VAL239
C	ASP241
C	GLN244

---

site_id	AF5
Number of Residues	6
Details	binding site for residue GOL C 406

Chain	Residue
C	GLU204
C	ASP205
C	ARG206
C	GLN207
C	TYR208
C	GLN244

---

site_id	AF6
Number of Residues	22
Details	binding site for Di-peptide TYR B 53 and TYR C 53

Chain	Residue
B	SER49
B	LYS50
B	ASP51
B	TYR52
B	PHE54
B	ASP55
B	SER56
B	TYR57
B	GLU171
B	ARG345
B	SAH401
C	SER49
C	LYS50
C	ASP51
C	TYR52
C	PHE54
C	ASP55
C	SER56
C	TYR57
C	GLU171
C	ARG345
C	SAH401

---

site_id	AF7
Number of Residues	22
Details	binding site for Di-peptide TYR B 53 and TYR C 53

Chain	Residue
B	SER49
B	LYS50
B	ASP51
B	TYR52
B	PHE54
B	ASP55
B	SER56
B	TYR57
B	GLU171
B	ARG345
B	SAH401
C	SER49
C	LYS50
C	ASP51
C	TYR52
C	PHE54
C	ASP55
C	SER56
C	TYR57
C	GLU171
C	ARG345
C	SAH401

---

site_id	AF8
Number of Residues	22
Details	binding site for Di-peptide TYR B 53 and TYR C 53

Chain	Residue
B	SER49
B	LYS50
B	ASP51
B	TYR52
B	PHE54
B	ASP55
B	SER56
B	TYR57
B	GLU171
B	ARG345
B	SAH401
C	SER49
C	LYS50
C	ASP51
C	TYR52
C	PHE54
C	ASP55
C	SER56
C	TYR57
C	GLU171
C	ARG345
C	SAH401

---

site_id	AF9
Number of Residues	22
Details	binding site for Di-peptide TYR B 53 and TYR C 53

Chain	Residue
B	SER49
B	LYS50
B	ASP51
B	TYR52
B	PHE54
B	ASP55
B	SER56
B	TYR57
B	GLU171
B	ARG345
B	SAH401
C	SER49
C	LYS50
C	ASP51
C	TYR52
C	PHE54
C	ASP55
C	SER56
C	TYR57
C	GLU171
C	ARG345
C	SAH401

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	ACT_SITE: ACT_SITE => ECO:0000250

Chain	Residue	Details
A	GLU162
A	GLU171
D	GLU162
D	GLU171
B	GLU162
B	GLU171
C	GLU162
C	GLU171

---

site_id	SWS_FT_FI2
Number of Residues	20
Details	BINDING: BINDING => ECO:0000250|UniProtKB:Q63009

Chain	Residue	Details
A	HIS63
D	GLU147
B	HIS63
B	ARG72
B	GLY96
B	GLU118
B	GLU147
C	HIS63
C	ARG72
C	GLY96
C	GLU118
A	ARG72
C	GLU147
A	GLY96
A	GLU118
A	GLU147
D	HIS63
D	ARG72
D	GLY96
D	GLU118

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	BINDING: BINDING => ECO:0000269|PubMed:31257072, ECO:0007744|PDB:6NT2

Chain	Residue	Details
A	VAL146
D	VAL146
B	VAL146
C	VAL146

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-succinyllysine => ECO:0000250|UniProtKB:Q9JIF0

Chain	Residue	Details
A	LYS134
D	LYS134
B	LYS134
C	LYS134

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q9JIF0

Chain	Residue	Details
A	LYS228
A	LYS233
D	LYS228
D	LYS233
B	LYS228
B	LYS233
C	LYS228
C	LYS233

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER304
A	SER307
D	SER304
D	SER307
B	SER304
B	SER307
C	SER304
C	SER307

---

site_id	SWS_FT_FI7
Number of Residues	8
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:Q9JIF0

Chain	Residue	Details
A	LYS145
D	LYS145
B	LYS145
C	LYS145

---