Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6NN8

The structure of human liver pyruvate kinase, hLPYK-S531E

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0001666	biological_process	response to hypoxia
A	0003824	molecular_function	catalytic activity
A	0004743	molecular_function	pyruvate kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006096	biological_process	glycolytic process
A	0007584	biological_process	response to nutrient
A	0008152	biological_process	metabolic process
A	0009749	biological_process	response to glucose
A	0010038	biological_process	response to metal ion
A	0016301	molecular_function	kinase activity
A	0016310	biological_process	phosphorylation
A	0016740	molecular_function	transferase activity
A	0030955	molecular_function	potassium ion binding
A	0032869	biological_process	cellular response to insulin stimulus
A	0033198	biological_process	response to ATP
A	0042866	biological_process	pyruvate biosynthetic process
A	0046872	molecular_function	metal ion binding
A	0048029	molecular_function	monosaccharide binding
A	0051591	biological_process	response to cAMP
A	0070062	cellular_component	extracellular exosome
A	0071872	biological_process	cellular response to epinephrine stimulus
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0001666	biological_process	response to hypoxia
B	0003824	molecular_function	catalytic activity
B	0004743	molecular_function	pyruvate kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006096	biological_process	glycolytic process
B	0007584	biological_process	response to nutrient
B	0008152	biological_process	metabolic process
B	0009749	biological_process	response to glucose
B	0010038	biological_process	response to metal ion
B	0016301	molecular_function	kinase activity
B	0016310	biological_process	phosphorylation
B	0016740	molecular_function	transferase activity
B	0030955	molecular_function	potassium ion binding
B	0032869	biological_process	cellular response to insulin stimulus
B	0033198	biological_process	response to ATP
B	0042866	biological_process	pyruvate biosynthetic process
B	0046872	molecular_function	metal ion binding
B	0048029	molecular_function	monosaccharide binding
B	0051591	biological_process	response to cAMP
B	0070062	cellular_component	extracellular exosome
B	0071872	biological_process	cellular response to epinephrine stimulus
C	0000166	molecular_function	nucleotide binding
C	0000287	molecular_function	magnesium ion binding
C	0001666	biological_process	response to hypoxia
C	0003824	molecular_function	catalytic activity
C	0004743	molecular_function	pyruvate kinase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006096	biological_process	glycolytic process
C	0007584	biological_process	response to nutrient
C	0008152	biological_process	metabolic process
C	0009749	biological_process	response to glucose
C	0010038	biological_process	response to metal ion
C	0016301	molecular_function	kinase activity
C	0016310	biological_process	phosphorylation
C	0016740	molecular_function	transferase activity
C	0030955	molecular_function	potassium ion binding
C	0032869	biological_process	cellular response to insulin stimulus
C	0033198	biological_process	response to ATP
C	0042866	biological_process	pyruvate biosynthetic process
C	0046872	molecular_function	metal ion binding
C	0048029	molecular_function	monosaccharide binding
C	0051591	biological_process	response to cAMP
C	0070062	cellular_component	extracellular exosome
C	0071872	biological_process	cellular response to epinephrine stimulus
D	0000166	molecular_function	nucleotide binding
D	0000287	molecular_function	magnesium ion binding
D	0001666	biological_process	response to hypoxia
D	0003824	molecular_function	catalytic activity
D	0004743	molecular_function	pyruvate kinase activity
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006096	biological_process	glycolytic process
D	0007584	biological_process	response to nutrient
D	0008152	biological_process	metabolic process
D	0009749	biological_process	response to glucose
D	0010038	biological_process	response to metal ion
D	0016301	molecular_function	kinase activity
D	0016310	biological_process	phosphorylation
D	0016740	molecular_function	transferase activity
D	0030955	molecular_function	potassium ion binding
D	0032869	biological_process	cellular response to insulin stimulus
D	0033198	biological_process	response to ATP
D	0042866	biological_process	pyruvate biosynthetic process
D	0046872	molecular_function	metal ion binding
D	0048029	molecular_function	monosaccharide binding
D	0051591	biological_process	response to cAMP
D	0070062	cellular_component	extracellular exosome
D	0071872	biological_process	cellular response to epinephrine stimulus
E	0000166	molecular_function	nucleotide binding
E	0000287	molecular_function	magnesium ion binding
E	0001666	biological_process	response to hypoxia
E	0003824	molecular_function	catalytic activity
E	0004743	molecular_function	pyruvate kinase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006096	biological_process	glycolytic process
E	0007584	biological_process	response to nutrient
E	0008152	biological_process	metabolic process
E	0009749	biological_process	response to glucose
E	0010038	biological_process	response to metal ion
E	0016301	molecular_function	kinase activity
E	0016310	biological_process	phosphorylation
E	0016740	molecular_function	transferase activity
E	0030955	molecular_function	potassium ion binding
E	0032869	biological_process	cellular response to insulin stimulus
E	0033198	biological_process	response to ATP
E	0042866	biological_process	pyruvate biosynthetic process
E	0046872	molecular_function	metal ion binding
E	0048029	molecular_function	monosaccharide binding
E	0051591	biological_process	response to cAMP
E	0070062	cellular_component	extracellular exosome
E	0071872	biological_process	cellular response to epinephrine stimulus
F	0000166	molecular_function	nucleotide binding
F	0000287	molecular_function	magnesium ion binding
F	0001666	biological_process	response to hypoxia
F	0003824	molecular_function	catalytic activity
F	0004743	molecular_function	pyruvate kinase activity
F	0005515	molecular_function	protein binding
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006096	biological_process	glycolytic process
F	0007584	biological_process	response to nutrient
F	0008152	biological_process	metabolic process
F	0009749	biological_process	response to glucose
F	0010038	biological_process	response to metal ion
F	0016301	molecular_function	kinase activity
F	0016310	biological_process	phosphorylation
F	0016740	molecular_function	transferase activity
F	0030955	molecular_function	potassium ion binding
F	0032869	biological_process	cellular response to insulin stimulus
F	0033198	biological_process	response to ATP
F	0042866	biological_process	pyruvate biosynthetic process
F	0046872	molecular_function	metal ion binding
F	0048029	molecular_function	monosaccharide binding
F	0051591	biological_process	response to cAMP
F	0070062	cellular_component	extracellular exosome
F	0071872	biological_process	cellular response to epinephrine stimulus
G	0000166	molecular_function	nucleotide binding
G	0000287	molecular_function	magnesium ion binding
G	0001666	biological_process	response to hypoxia
G	0003824	molecular_function	catalytic activity
G	0004743	molecular_function	pyruvate kinase activity
G	0005515	molecular_function	protein binding
G	0005524	molecular_function	ATP binding
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006096	biological_process	glycolytic process
G	0007584	biological_process	response to nutrient
G	0008152	biological_process	metabolic process
G	0009749	biological_process	response to glucose
G	0010038	biological_process	response to metal ion
G	0016301	molecular_function	kinase activity
G	0016310	biological_process	phosphorylation
G	0016740	molecular_function	transferase activity
G	0030955	molecular_function	potassium ion binding
G	0032869	biological_process	cellular response to insulin stimulus
G	0033198	biological_process	response to ATP
G	0042866	biological_process	pyruvate biosynthetic process
G	0046872	molecular_function	metal ion binding
G	0048029	molecular_function	monosaccharide binding
G	0051591	biological_process	response to cAMP
G	0070062	cellular_component	extracellular exosome
G	0071872	biological_process	cellular response to epinephrine stimulus
H	0000166	molecular_function	nucleotide binding
H	0000287	molecular_function	magnesium ion binding
H	0001666	biological_process	response to hypoxia
H	0003824	molecular_function	catalytic activity
H	0004743	molecular_function	pyruvate kinase activity
H	0005515	molecular_function	protein binding
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006096	biological_process	glycolytic process
H	0007584	biological_process	response to nutrient
H	0008152	biological_process	metabolic process
H	0009749	biological_process	response to glucose
H	0010038	biological_process	response to metal ion
H	0016301	molecular_function	kinase activity
H	0016310	biological_process	phosphorylation
H	0016740	molecular_function	transferase activity
H	0030955	molecular_function	potassium ion binding
H	0032869	biological_process	cellular response to insulin stimulus
H	0033198	biological_process	response to ATP
H	0042866	biological_process	pyruvate biosynthetic process
H	0046872	molecular_function	metal ion binding
H	0048029	molecular_function	monosaccharide binding
H	0051591	biological_process	response to cAMP
H	0070062	cellular_component	extracellular exosome
H	0071872	biological_process	cellular response to epinephrine stimulus

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue EDO A 601

Chain	Residue
A	SER58
A	ASN82
A	HIS476
A	VAL481
A	PHE482
A	HOH742

site_id	AC2
Number of Residues	2
Details	binding site for residue EDO A 602

Chain	Residue
A	HIS93
A	ARG239

site_id	AC3
Number of Residues	4
Details	binding site for residue EDO A 603

Chain	Residue
A	SER69
A	ASN102
A	HOH744
A	ARG68

site_id	AC4
Number of Residues	5
Details	binding site for residue EDO A 604

Chain	Residue
A	VAL107
A	PHE110
A	SER116
A	TYR117
A	ARG118

site_id	AC5
Number of Residues	4
Details	binding site for residue EDO A 605

Chain	Residue
A	ARG85
A	ALA305
A	ASP308
A	HOH754

site_id	AC6
Number of Residues	4
Details	binding site for residue EDO A 606

Chain	Residue
A	LYS278
A	GLN451
A	GLN474
A	HOH740

site_id	AC7
Number of Residues	5
Details	binding site for residue EDO A 607

Chain	Residue
A	VAL245
A	GLY248
A	VAL249
A	GLU273
A	GLN470

site_id	AC8
Number of Residues	7
Details	binding site for residue EDO A 608

Chain	Residue
A	GLY333
A	LYS334
A	PRO335
A	ASP369
A	ARG455
A	ARG457
A	HOH723

site_id	AC9
Number of Residues	4
Details	binding site for residue EDO A 609

Chain	Residue
A	VAL539
A	SER541
D	PRO420
D	ASN535

site_id	AD1
Number of Residues	4
Details	binding site for residue EDO A 610

Chain	Residue
A	PRO65
A	ALA66
A	SER69
A	ARG72

site_id	AD2
Number of Residues	3
Details	binding site for residue EDO B 601

Chain	Residue
B	LYS278
B	GLN451
B	HOH728

site_id	AD3
Number of Residues	4
Details	binding site for residue EDO B 602

Chain	Residue
B	PHE24
B	GLN28
B	CYS436
B	ALA437

site_id	AD4
Number of Residues	7
Details	binding site for residue EDO B 603

Chain	Residue
B	ARG85
B	LYS282
B	GLU284
B	ALA305
B	GLY307
B	THR340
B	HOH708

site_id	AD5
Number of Residues	5
Details	binding site for residue EDO B 604

Chain	Residue
B	GLN28
B	PRO51
B	ALA53
B	ARG395
B	ALA399

site_id	AD6
Number of Residues	7
Details	binding site for residue EDO B 605

Chain	Residue
B	LEU443
B	THR444
B	THR445
B	VAL465
B	THR466
B	ARG467
B	ARG501

site_id	AD7
Number of Residues	5
Details	binding site for residue EDO B 606

Chain	Residue
A	ASP46
B	VAL289
B	LYS290
B	MET324
B	ARG328

site_id	AD8
Number of Residues	2
Details	binding site for residue EDO B 607

Chain	Residue
B	ARG132
B	GLY220

site_id	AD9
Number of Residues	7
Details	binding site for residue EDO C 601

Chain	Residue
C	LEU443
C	THR445
C	TRP494
C	ARG501
C	GLY530
C	GLU531
C	HOH715

site_id	AE1
Number of Residues	5
Details	binding site for residue EDO C 602

Chain	Residue
C	ARG85
C	ALA305
C	GLY307
C	ASP308
C	THR340

site_id	AE2
Number of Residues	6
Details	binding site for residue EDO C 603

Chain	Residue
C	ARG55
C	SER58
C	ASN82
C	HIS476
C	VAL481
C	PHE482

site_id	AE3
Number of Residues	5
Details	binding site for residue EDO C 604

Chain	Residue
C	ASP300
C	LYS334
C	ARG267
C	VAL298
C	SER299

site_id	AE4
Number of Residues	4
Details	binding site for residue EDO C 605

Chain	Residue
C	LYS278
C	GLY447
C	GLN451
C	GLN474

site_id	AE5
Number of Residues	5
Details	binding site for residue EDO C 606

Chain	Residue
C	ASN381
C	PHE382
C	PRO383
C	VAL384
C	GLU385

site_id	AE6
Number of Residues	6
Details	binding site for residue EDO D 601

Chain	Residue
D	ARG85
D	LYS282
D	GLU284
D	GLY307
D	ASP308
D	THR340

site_id	AE7
Number of Residues	3
Details	binding site for residue EDO D 602

Chain	Residue
A	PRO420
D	VAL539
D	SER541

site_id	AE8
Number of Residues	7
Details	binding site for residue EDO D 603

Chain	Residue
D	GLN28
D	ALA53
D	ALA437
D	ALA438
D	ARG459
D	ALA460
D	ALA461

site_id	AE9
Number of Residues	6
Details	binding site for residue EDO D 604

Chain	Residue
D	ALA495
D	VAL498
D	THR525
D	GLY526
D	ILE536
D	ARG538

site_id	AF1
Number of Residues	9
Details	binding site for residue EDO E 601

Chain	Residue
E	GLY333
E	LYS334
E	PRO335
E	ASP369
E	GLN451
E	SER454
E	ARG455
E	ARG457
E	CYS478

site_id	AF2
Number of Residues	6
Details	binding site for residue EDO E 602

Chain	Residue
E	ASN222
E	LEU223
E	ALA226
E	PHE256
E	ASN285
E	GLU312

site_id	AF3
Number of Residues	6
Details	binding site for residue EDO E 603

Chain	Residue
E	SER56
E	SER58
E	ASN82
E	HIS476
E	VAL481
E	PHE482

site_id	AF4
Number of Residues	7
Details	binding site for residue EDO E 604

Chain	Residue
E	ARG85
E	LYS282
E	MET303
E	ALA305
E	GLY307
E	ASP308
E	THR340

site_id	AF5
Number of Residues	2
Details	binding site for residue EDO E 605

Chain	Residue
E	LYS278
E	GLN451

site_id	AF6
Number of Residues	3
Details	binding site for residue EDO E 606

Chain	Residue
E	GLU297
E	SER299
E	LYS334

site_id	AF7
Number of Residues	4
Details	binding site for residue EDO E 607

Chain	Residue
E	ALA33
E	HIS403
E	ARG404
E	ARG459

site_id	AF8
Number of Residues	1
Details	binding site for residue EDO F 601

Chain	Residue
F	ARG459

site_id	AF9
Number of Residues	4
Details	binding site for residue EDO F 602

Chain	Residue
F	ARG85
F	LYS282
F	GLU284
F	ALA305

site_id	AG1
Number of Residues	8
Details	binding site for residue EDO G 601

Chain	Residue
G	GLN28
G	PRO51
G	VAL52
G	ALA53
G	ARG395
G	GLU398
G	ALA399
G	HOH704

site_id	AG2
Number of Residues	2
Details	binding site for residue EDO G 602

Chain	Residue
G	ARG404
G	ARG459

site_id	AG3
Number of Residues	5
Details	binding site for residue EDO H 601

Chain	Residue
H	LYS282
H	ALA305
H	GLY307
H	ASP308
H	THR340

site_id	AG4
Number of Residues	7
Details	binding site for residue EDO H 602

Chain	Residue
H	GLY333
H	PRO335
H	ASP369
H	SER454
H	ARG455
H	ARG457
H	CYS478

site_id	AG5
Number of Residues	1
Details	binding site for residue EDO H 603

Chain	Residue
H	GLU40

Functional Information from PROSITE/UniProt

site_id	PS00110
Number of Residues	13
Details	PYRUVATE_KINASE Pyruvate kinase active site signature. IkIISKIENhEGV

Chain	Residue	Details
A	ILE277-VAL289

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269\|PubMed:11960989, ECO:0007744\|PDB:2VGB

Chain	Residue	Details
E	ARG85
E	SER89
F	ARG85
F	SER89
G	ARG85
G	SER89
H	ARG85
H	SER89
A	ARG85
A	SER89
B	ARG85
C	ARG85
C	SER89
D	ARG85
D	SER89
B	SER89

site_id	SWS_FT_FI2
Number of Residues	96
Details	BINDING: BINDING => ECO:0000269\|PubMed:11960989, ECO:0007744\|PDB:2VGF

Chain	Residue	Details
B	GLU284
B	GLY307
B	ASP308
B	THR340
B	THR444
B	TRP494
B	ARG501
B	ARG528
C	ASN87
C	ASP125
C	THR126
C	LYS282
C	GLU284
C	GLY307
C	ASP308
C	THR340
C	THR444
C	TRP494
C	ARG501
C	ARG528
D	ASN87
D	ASP125
D	THR126
D	LYS282
D	GLU284
D	GLY307
D	ASP308
D	THR340
D	THR444
D	TRP494
D	ARG501
D	ARG528
E	ASN87
E	ASP125
E	THR126
E	LYS282
E	GLU284
E	GLY307
E	ASP308
E	THR340
E	THR444
E	TRP494
E	ARG501
E	ARG528
F	ASN87
F	ASP125
F	THR126
F	LYS282
F	GLU284
F	GLY307
F	ASP308
F	THR340
F	THR444
F	TRP494
F	ARG501
F	ARG528
G	ASN87
G	ASP125
G	THR126
G	LYS282
G	GLU284
G	GLY307
G	ASP308
G	THR340
G	THR444
G	TRP494
G	ARG501
G	ARG528
H	ASN87
H	ASP125
H	THR126
H	LYS282
H	GLU284
H	GLY307
H	ASP308
H	THR340
H	THR444
H	TRP494
H	ARG501
H	ARG528
A	THR126
A	LYS282
A	GLU284
A	GLY307
A	ASP308
A	THR340
A	THR444
A	TRP494
A	ARG501
A	ARG528
B	ASN87
B	ASP125
B	THR126
B	LYS282
A	ASN87
A	ASP125

site_id	SWS_FT_FI3
Number of Residues	16
Details	BINDING: BINDING => ECO:0000250\|UniProtKB:P14618

Chain	Residue	Details
E	ARG132
E	LYS219
F	ARG132
F	LYS219
G	ARG132
G	LYS219
H	ARG132
H	LYS219
A	LYS219
B	ARG132
C	ARG132
C	LYS219
D	ARG132
D	LYS219
A	ARG132
B	LYS219

site_id	SWS_FT_FI4
Number of Residues	8
Details	SITE: Transition state stabilizer => ECO:0000250\|UniProtKB:P00549

Chain	Residue	Details
B	LYS282
C	LYS282
D	LYS282
E	LYS282
F	LYS282
G	LYS282
H	LYS282
A	LYS282

site_id	SWS_FT_FI5
Number of Residues	16
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:24275569

Chain	Residue	Details
B	SER12
B	SER261
C	SER12
C	SER261
E	SER12
E	SER261
F	SER12
F	SER261
G	SER12
G	SER261
H	SER12
H	SER261
A	SER12
A	SER261
D	SER12
D	SER261

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)