Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6NMI

Cryo-EM structure of the human TFIIH core complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003678	molecular_function	DNA helicase activity
A	0005524	molecular_function	ATP binding
A	0006289	biological_process	nucleotide-excision repair
A	0006367	biological_process	transcription initiation at RNA polymerase II promoter
A	0016787	molecular_function	hydrolase activity
B	0000166	molecular_function	nucleotide binding
B	0000439	cellular_component	transcription factor TFIIH core complex
B	0003676	molecular_function	nucleic acid binding
B	0003677	molecular_function	DNA binding
B	0003678	molecular_function	DNA helicase activity
B	0003684	molecular_function	damaged DNA binding
B	0004386	molecular_function	helicase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005669	cellular_component	transcription factor TFIID complex
B	0005675	cellular_component	transcription factor TFIIH holo complex
B	0005737	cellular_component	cytoplasm
B	0005819	cellular_component	spindle
B	0006139	biological_process	nucleobase-containing compound metabolic process
B	0006281	biological_process	DNA repair
B	0006283	biological_process	transcription-coupled nucleotide-excision repair
B	0006289	biological_process	nucleotide-excision repair
B	0006351	biological_process	DNA-templated transcription
B	0006357	biological_process	regulation of transcription by RNA polymerase II
B	0006366	biological_process	transcription by RNA polymerase II
B	0006367	biological_process	transcription initiation at RNA polymerase II promoter
B	0006915	biological_process	apoptotic process
B	0006974	biological_process	DNA damage response
B	0006979	biological_process	response to oxidative stress
B	0007059	biological_process	chromosome segregation
B	0016787	molecular_function	hydrolase activity
B	0016818	molecular_function	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
B	0016853	molecular_function	isomerase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0030674	molecular_function	protein-macromolecule adaptor activity
B	0035315	biological_process	hair cell differentiation
B	0043139	molecular_function	5'-3' DNA helicase activity
B	0045951	biological_process	positive regulation of mitotic recombination
B	0046872	molecular_function	metal ion binding
B	0051536	molecular_function	iron-sulfur cluster binding
B	0051539	molecular_function	4 iron, 4 sulfur cluster binding
B	0070516	cellular_component	CAK-ERCC2 complex
B	0071817	cellular_component	MMXD complex
B	1901990	biological_process	regulation of mitotic cell cycle phase transition
C	0000079	biological_process	regulation of cyclin-dependent protein serine/threonine kinase activity
C	0000439	cellular_component	transcription factor TFIIH core complex
C	0003682	molecular_function	chromatin binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005675	cellular_component	transcription factor TFIIH holo complex
C	0006281	biological_process	DNA repair
C	0006289	biological_process	nucleotide-excision repair
C	0006351	biological_process	DNA-templated transcription
C	0006360	biological_process	transcription by RNA polymerase I
C	0006366	biological_process	transcription by RNA polymerase II
C	0006367	biological_process	transcription initiation at RNA polymerase II promoter
C	0006974	biological_process	DNA damage response
C	0009755	biological_process	hormone-mediated signaling pathway
C	0045893	biological_process	positive regulation of DNA-templated transcription
C	0046966	molecular_function	nuclear thyroid hormone receptor binding
D	0000438	cellular_component	core TFIIH complex portion of holo TFIIH complex
D	0000439	cellular_component	transcription factor TFIIH core complex
D	0001671	molecular_function	ATPase activator activity
D	0003690	molecular_function	double-stranded DNA binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005669	cellular_component	transcription factor TFIID complex
D	0005675	cellular_component	transcription factor TFIIH holo complex
D	0006281	biological_process	DNA repair
D	0006289	biological_process	nucleotide-excision repair
D	0006351	biological_process	DNA-templated transcription
D	0006366	biological_process	transcription by RNA polymerase II
D	0006367	biological_process	transcription initiation at RNA polymerase II promoter
D	0006974	biological_process	DNA damage response
D	0016251	molecular_function	RNA polymerase II general transcription initiation factor activity
D	0016607	cellular_component	nuclear speck
E	0000439	cellular_component	transcription factor TFIIH core complex
E	0006281	biological_process	DNA repair
E	0006289	biological_process	nucleotide-excision repair
E	0006351	biological_process	DNA-templated transcription
E	0008270	molecular_function	zinc ion binding
F	0000438	cellular_component	core TFIIH complex portion of holo TFIIH complex
F	0000439	cellular_component	transcription factor TFIIH core complex
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005669	cellular_component	transcription factor TFIID complex
F	0005675	cellular_component	transcription factor TFIIH holo complex
F	0006281	biological_process	DNA repair
F	0006289	biological_process	nucleotide-excision repair
F	0006351	biological_process	DNA-templated transcription
F	0006355	biological_process	regulation of DNA-templated transcription
F	0006366	biological_process	transcription by RNA polymerase II
F	0006367	biological_process	transcription initiation at RNA polymerase II promoter
F	0006974	biological_process	DNA damage response
F	0008270	molecular_function	zinc ion binding
F	0016251	molecular_function	RNA polymerase II general transcription initiation factor activity
F	0046872	molecular_function	metal ion binding
F	0097550	cellular_component	transcription preinitiation complex
G	0000439	cellular_component	transcription factor TFIIH core complex
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005669	cellular_component	transcription factor TFIID complex
G	0005675	cellular_component	transcription factor TFIIH holo complex
G	0005730	cellular_component	nucleolus
G	0005737	cellular_component	cytoplasm
G	0006281	biological_process	DNA repair
G	0006289	biological_process	nucleotide-excision repair
G	0006294	biological_process	nucleotide-excision repair, preincision complex assembly
G	0006351	biological_process	DNA-templated transcription
G	0006366	biological_process	transcription by RNA polymerase II
G	0006367	biological_process	transcription initiation at RNA polymerase II promoter
G	0006974	biological_process	DNA damage response
H	0000082	biological_process	G1/S transition of mitotic cell cycle
H	0000439	cellular_component	transcription factor TFIIH core complex
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005654	cellular_component	nucleoplasm
H	0005675	cellular_component	transcription factor TFIIH holo complex
H	0006281	biological_process	DNA repair
H	0006289	biological_process	nucleotide-excision repair
H	0006351	biological_process	DNA-templated transcription
H	0006357	biological_process	regulation of transcription by RNA polymerase II
H	0006367	biological_process	transcription initiation at RNA polymerase II promoter
H	0008270	molecular_function	zinc ion binding
H	0043066	biological_process	negative regulation of apoptotic process
H	0045737	biological_process	positive regulation of cyclin-dependent protein serine/threonine kinase activity
H	0046872	molecular_function	metal ion binding
H	0048661	biological_process	positive regulation of smooth muscle cell proliferation
H	0061575	molecular_function	cyclin-dependent protein serine/threonine kinase activator activity
H	0070516	cellular_component	CAK-ERCC2 complex
H	0070985	cellular_component	transcription factor TFIIK complex
H	2000045	biological_process	regulation of G1/S transition of mitotic cell cycle

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue SF4 B 1000

Chain	Residue
B	CYS116
B	VAL121
B	CYS134
B	CYS155
B	PHE157
B	CYS190

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN E 401

Chain	Residue
E	CYS371
E	CYS345
E	CYS348
E	CYS368

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN E 402

Chain	Residue
E	CYS360
E	CYS363
E	CYS382
E	CYS385

site_id	AC4
Number of Residues	5
Details	binding site for residue ZN E 403

Chain	Residue
E	CYS291
E	GLN293
E	CYS294
E	CYS305
E	CYS308

site_id	AC5
Number of Residues	5
Details	binding site for residue ZN F 401

Chain	Residue
F	CYS268
F	CYS271
F	CYS282
F	CYS285
F	THR287

site_id	AC6
Number of Residues	4
Details	binding site for residue ZN H 400

Chain	Residue
H	CYS6
H	CYS9
H	CYS31
H	CYS34

site_id	AC7
Number of Residues	4
Details	binding site for residue ZN H 401

Chain	Residue
H	CYS26
H	HIS28
H	CYS46
H	CYS49

Functional Information from PROSITE/UniProt

site_id	PS00028
Number of Residues	21
Details	ZINC_FINGER_C2H2_1 Zinc finger C2H2 type domain signature. Cav..CqnvFcvdcdvfvHdsl..H

Chain	Residue	Details
E	CYS360-HIS380

site_id	PS00518
Number of Residues	10
Details	ZF_RING_1 Zinc finger RING-type signature. CgHtLCesCV

Chain	Residue	Details
H	CYS26-VAL35

site_id	PS00690
Number of Residues	10
Details	DEAH_ATP_HELICASE DEAH-box subfamily ATP-dependent helicases signature. AvVVFDEAHN

Chain	Residue	Details
B	ALA229-ASN238

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	437
Details	Domain: {"description":"Helicase ATP-binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	3
Details	Motif: {"description":"DEVH box"}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"33902107","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7NVV","evidenceCode":"ECO:0000312"},{"source":"PDB","id":"7NVX","evidenceCode":"ECO:0000312"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"33902107","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"7NVV","evidenceCode":"ECO:0000312"},{"source":"PDB","id":"7NVX","evidenceCode":"ECO:0000312"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	199
Details	Region: {"description":"Mediates interaction with MMS19"}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	3
Details	Motif: {"description":"DEAH box"}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	7
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"31253769","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"6RO4","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	176
Details	Domain: {"description":"VWFA","evidences":[{"source":"PROSITE-ProRule","id":"PRU00219","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	1
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"A0JN27","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	17
Details	Zinc finger: {"description":"C4-type"}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	19
Details	Domain: {"description":"UIM","evidences":[{"source":"PROSITE-ProRule","id":"PRU00213","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	44
Details	Zinc finger: {"description":"RING-type","evidences":[{"source":"PROSITE-ProRule","id":"PRU00175","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	1
Details	Modified residue: {"description":"N-acetylmethionine","evidences":[{"source":"PubMed","id":"19413330","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	1
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18691976","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)