6N7K

Cryo-EM structure of tetrameric Ptch1 in complex with ShhNp (form II)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0001658	biological_process	branching involved in ureteric bud morphogenesis
A	0001701	biological_process	in utero embryonic development
A	0001709	biological_process	cell fate determination
A	0001841	biological_process	neural tube formation
A	0001843	biological_process	neural tube closure
A	0003007	biological_process	heart morphogenesis
A	0005113	molecular_function	patched binding
A	0005119	molecular_function	smoothened binding
A	0005515	molecular_function	protein binding
A	0005794	cellular_component	Golgi apparatus
A	0005886	cellular_component	plasma membrane
A	0005901	cellular_component	caveola
A	0005929	cellular_component	cilium
A	0007165	biological_process	signal transduction
A	0007286	biological_process	spermatid development
A	0007346	biological_process	regulation of mitotic cell cycle
A	0007389	biological_process	pattern specification process
A	0007420	biological_process	brain development
A	0008158	molecular_function	hedgehog receptor activity
A	0008201	molecular_function	heparin binding
A	0008285	biological_process	negative regulation of cell population proliferation
A	0008544	biological_process	epidermis development
A	0008589	biological_process	regulation of smoothened signaling pathway
A	0009410	biological_process	response to xenobiotic stimulus
A	0009612	biological_process	response to mechanical stimulus
A	0009887	biological_process	animal organ morphogenesis
A	0009953	biological_process	dorsal/ventral pattern formation
A	0009957	biological_process	epidermal cell fate specification
A	0010157	biological_process	response to chlorate
A	0010839	biological_process	negative regulation of keratinocyte proliferation
A	0010875	biological_process	positive regulation of cholesterol efflux
A	0015485	molecular_function	cholesterol binding
A	0016020	cellular_component	membrane
A	0016485	biological_process	protein processing
A	0021522	biological_process	spinal cord motor neuron differentiation
A	0021532	biological_process	neural tube patterning
A	0021904	biological_process	dorsal/ventral neural tube patterning
A	0021997	biological_process	neural plate axis specification
A	0030326	biological_process	embryonic limb morphogenesis
A	0030332	molecular_function	cyclin binding
A	0030496	cellular_component	midbody
A	0030666	cellular_component	endocytic vesicle membrane
A	0030850	biological_process	prostate gland development
A	0030879	biological_process	mammary gland development
A	0032355	biological_process	response to estradiol
A	0032526	biological_process	response to retinoic acid
A	0032880	biological_process	regulation of protein localization
A	0035108	biological_process	limb morphogenesis
A	0035137	biological_process	hindlimb morphogenesis
A	0040008	biological_process	regulation of growth
A	0040015	biological_process	negative regulation of multicellular organism growth
A	0042127	biological_process	regulation of cell population proliferation
A	0042593	biological_process	glucose homeostasis
A	0043279	biological_process	response to alkaloid
A	0043616	biological_process	keratinocyte proliferation
A	0044294	cellular_component	dendritic growth cone
A	0044295	cellular_component	axonal growth cone
A	0044877	molecular_function	protein-containing complex binding
A	0045177	cellular_component	apical part of cell
A	0045211	cellular_component	postsynaptic membrane
A	0045606	biological_process	positive regulation of epidermal cell differentiation
A	0045668	biological_process	negative regulation of osteoblast differentiation
A	0045879	biological_process	negative regulation of smoothened signaling pathway
A	0045892	biological_process	negative regulation of DNA-templated transcription
A	0045893	biological_process	positive regulation of DNA-templated transcription
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0048568	biological_process	embryonic organ development
A	0048745	biological_process	smooth muscle tissue development
A	0050673	biological_process	epithelial cell proliferation
A	0050680	biological_process	negative regulation of epithelial cell proliferation
A	0051782	biological_process	negative regulation of cell division
A	0060037	biological_process	pharyngeal system development
A	0060170	cellular_component	ciliary membrane
A	0060603	biological_process	mammary gland duct morphogenesis
A	0060644	biological_process	mammary gland epithelial cell differentiation
A	0061005	biological_process	cell differentiation involved in kidney development
A	0061053	biological_process	somite development
A	0071397	biological_process	cellular response to cholesterol
A	0071679	biological_process	commissural neuron axon guidance
A	0072089	biological_process	stem cell proliferation
A	0072203	biological_process	cell proliferation involved in metanephros development
A	0072205	biological_process	metanephric collecting duct development
A	0072659	biological_process	protein localization to plasma membrane
A	0097108	molecular_function	hedgehog family protein binding
A	0097421	biological_process	liver regeneration
A	2000647	biological_process	negative regulation of stem cell proliferation
B	0000122	biological_process	negative regulation of transcription by RNA polymerase II
B	0001658	biological_process	branching involved in ureteric bud morphogenesis
B	0001701	biological_process	in utero embryonic development
B	0001709	biological_process	cell fate determination
B	0001841	biological_process	neural tube formation
B	0001843	biological_process	neural tube closure
B	0003007	biological_process	heart morphogenesis
B	0005113	molecular_function	patched binding
B	0005119	molecular_function	smoothened binding
B	0005515	molecular_function	protein binding
B	0005794	cellular_component	Golgi apparatus
B	0005886	cellular_component	plasma membrane
B	0005901	cellular_component	caveola
B	0005929	cellular_component	cilium
B	0007165	biological_process	signal transduction
B	0007286	biological_process	spermatid development
B	0007346	biological_process	regulation of mitotic cell cycle
B	0007389	biological_process	pattern specification process
B	0007420	biological_process	brain development
B	0008158	molecular_function	hedgehog receptor activity
B	0008201	molecular_function	heparin binding
B	0008285	biological_process	negative regulation of cell population proliferation
B	0008544	biological_process	epidermis development
B	0008589	biological_process	regulation of smoothened signaling pathway
B	0009410	biological_process	response to xenobiotic stimulus
B	0009612	biological_process	response to mechanical stimulus
B	0009887	biological_process	animal organ morphogenesis
B	0009953	biological_process	dorsal/ventral pattern formation
B	0009957	biological_process	epidermal cell fate specification
B	0010157	biological_process	response to chlorate
B	0010839	biological_process	negative regulation of keratinocyte proliferation
B	0010875	biological_process	positive regulation of cholesterol efflux
B	0015485	molecular_function	cholesterol binding
B	0016020	cellular_component	membrane
B	0016485	biological_process	protein processing
B	0021522	biological_process	spinal cord motor neuron differentiation
B	0021532	biological_process	neural tube patterning
B	0021904	biological_process	dorsal/ventral neural tube patterning
B	0021997	biological_process	neural plate axis specification
B	0030326	biological_process	embryonic limb morphogenesis
B	0030332	molecular_function	cyclin binding
B	0030496	cellular_component	midbody
B	0030666	cellular_component	endocytic vesicle membrane
B	0030850	biological_process	prostate gland development
B	0030879	biological_process	mammary gland development
B	0032355	biological_process	response to estradiol
B	0032526	biological_process	response to retinoic acid
B	0032880	biological_process	regulation of protein localization
B	0035108	biological_process	limb morphogenesis
B	0035137	biological_process	hindlimb morphogenesis
B	0040008	biological_process	regulation of growth
B	0040015	biological_process	negative regulation of multicellular organism growth
B	0042127	biological_process	regulation of cell population proliferation
B	0042593	biological_process	glucose homeostasis
B	0043279	biological_process	response to alkaloid
B	0043616	biological_process	keratinocyte proliferation
B	0044294	cellular_component	dendritic growth cone
B	0044295	cellular_component	axonal growth cone
B	0044877	molecular_function	protein-containing complex binding
B	0045177	cellular_component	apical part of cell
B	0045211	cellular_component	postsynaptic membrane
B	0045606	biological_process	positive regulation of epidermal cell differentiation
B	0045668	biological_process	negative regulation of osteoblast differentiation
B	0045879	biological_process	negative regulation of smoothened signaling pathway
B	0045892	biological_process	negative regulation of DNA-templated transcription
B	0045893	biological_process	positive regulation of DNA-templated transcription
B	0048471	cellular_component	perinuclear region of cytoplasm
B	0048568	biological_process	embryonic organ development
B	0048745	biological_process	smooth muscle tissue development
B	0050673	biological_process	epithelial cell proliferation
B	0050680	biological_process	negative regulation of epithelial cell proliferation
B	0051782	biological_process	negative regulation of cell division
B	0060037	biological_process	pharyngeal system development
B	0060170	cellular_component	ciliary membrane
B	0060603	biological_process	mammary gland duct morphogenesis
B	0060644	biological_process	mammary gland epithelial cell differentiation
B	0061005	biological_process	cell differentiation involved in kidney development
B	0061053	biological_process	somite development
B	0071397	biological_process	cellular response to cholesterol
B	0071679	biological_process	commissural neuron axon guidance
B	0072089	biological_process	stem cell proliferation
B	0072203	biological_process	cell proliferation involved in metanephros development
B	0072205	biological_process	metanephric collecting duct development
B	0072659	biological_process	protein localization to plasma membrane
B	0097108	molecular_function	hedgehog family protein binding
B	0097421	biological_process	liver regeneration
B	2000647	biological_process	negative regulation of stem cell proliferation
C	0007267	biological_process	cell-cell signaling
C	0007275	biological_process	multicellular organism development
D	0000122	biological_process	negative regulation of transcription by RNA polymerase II
D	0001658	biological_process	branching involved in ureteric bud morphogenesis
D	0001701	biological_process	in utero embryonic development
D	0001709	biological_process	cell fate determination
D	0001841	biological_process	neural tube formation
D	0001843	biological_process	neural tube closure
D	0003007	biological_process	heart morphogenesis
D	0005113	molecular_function	patched binding
D	0005119	molecular_function	smoothened binding
D	0005515	molecular_function	protein binding
D	0005794	cellular_component	Golgi apparatus
D	0005886	cellular_component	plasma membrane
D	0005901	cellular_component	caveola
D	0005929	cellular_component	cilium
D	0007165	biological_process	signal transduction
D	0007286	biological_process	spermatid development
D	0007346	biological_process	regulation of mitotic cell cycle
D	0007389	biological_process	pattern specification process
D	0007420	biological_process	brain development
D	0008158	molecular_function	hedgehog receptor activity
D	0008201	molecular_function	heparin binding
D	0008285	biological_process	negative regulation of cell population proliferation
D	0008544	biological_process	epidermis development
D	0008589	biological_process	regulation of smoothened signaling pathway
D	0009410	biological_process	response to xenobiotic stimulus
D	0009612	biological_process	response to mechanical stimulus
D	0009887	biological_process	animal organ morphogenesis
D	0009953	biological_process	dorsal/ventral pattern formation
D	0009957	biological_process	epidermal cell fate specification
D	0010157	biological_process	response to chlorate
D	0010839	biological_process	negative regulation of keratinocyte proliferation
D	0010875	biological_process	positive regulation of cholesterol efflux
D	0015485	molecular_function	cholesterol binding
D	0016020	cellular_component	membrane
D	0016485	biological_process	protein processing
D	0021522	biological_process	spinal cord motor neuron differentiation
D	0021532	biological_process	neural tube patterning
D	0021904	biological_process	dorsal/ventral neural tube patterning
D	0021997	biological_process	neural plate axis specification
D	0030326	biological_process	embryonic limb morphogenesis
D	0030332	molecular_function	cyclin binding
D	0030496	cellular_component	midbody
D	0030666	cellular_component	endocytic vesicle membrane
D	0030850	biological_process	prostate gland development
D	0030879	biological_process	mammary gland development
D	0032355	biological_process	response to estradiol
D	0032526	biological_process	response to retinoic acid
D	0032880	biological_process	regulation of protein localization
D	0035108	biological_process	limb morphogenesis
D	0035137	biological_process	hindlimb morphogenesis
D	0040008	biological_process	regulation of growth
D	0040015	biological_process	negative regulation of multicellular organism growth
D	0042127	biological_process	regulation of cell population proliferation
D	0042593	biological_process	glucose homeostasis
D	0043279	biological_process	response to alkaloid
D	0043616	biological_process	keratinocyte proliferation
D	0044294	cellular_component	dendritic growth cone
D	0044295	cellular_component	axonal growth cone
D	0044877	molecular_function	protein-containing complex binding
D	0045177	cellular_component	apical part of cell
D	0045211	cellular_component	postsynaptic membrane
D	0045606	biological_process	positive regulation of epidermal cell differentiation
D	0045668	biological_process	negative regulation of osteoblast differentiation
D	0045879	biological_process	negative regulation of smoothened signaling pathway
D	0045892	biological_process	negative regulation of DNA-templated transcription
D	0045893	biological_process	positive regulation of DNA-templated transcription
D	0048471	cellular_component	perinuclear region of cytoplasm
D	0048568	biological_process	embryonic organ development
D	0048745	biological_process	smooth muscle tissue development
D	0050673	biological_process	epithelial cell proliferation
D	0050680	biological_process	negative regulation of epithelial cell proliferation
D	0051782	biological_process	negative regulation of cell division
D	0060037	biological_process	pharyngeal system development
D	0060170	cellular_component	ciliary membrane
D	0060603	biological_process	mammary gland duct morphogenesis
D	0060644	biological_process	mammary gland epithelial cell differentiation
D	0061005	biological_process	cell differentiation involved in kidney development
D	0061053	biological_process	somite development
D	0071397	biological_process	cellular response to cholesterol
D	0071679	biological_process	commissural neuron axon guidance
D	0072089	biological_process	stem cell proliferation
D	0072203	biological_process	cell proliferation involved in metanephros development
D	0072205	biological_process	metanephric collecting duct development
D	0072659	biological_process	protein localization to plasma membrane
D	0097108	molecular_function	hedgehog family protein binding
D	0097421	biological_process	liver regeneration
D	2000647	biological_process	negative regulation of stem cell proliferation
E	0000122	biological_process	negative regulation of transcription by RNA polymerase II
E	0001658	biological_process	branching involved in ureteric bud morphogenesis
E	0001701	biological_process	in utero embryonic development
E	0001709	biological_process	cell fate determination
E	0001841	biological_process	neural tube formation
E	0001843	biological_process	neural tube closure
E	0003007	biological_process	heart morphogenesis
E	0005113	molecular_function	patched binding
E	0005119	molecular_function	smoothened binding
E	0005515	molecular_function	protein binding
E	0005794	cellular_component	Golgi apparatus
E	0005886	cellular_component	plasma membrane
E	0005901	cellular_component	caveola
E	0005929	cellular_component	cilium
E	0007165	biological_process	signal transduction
E	0007286	biological_process	spermatid development
E	0007346	biological_process	regulation of mitotic cell cycle
E	0007389	biological_process	pattern specification process
E	0007420	biological_process	brain development
E	0008158	molecular_function	hedgehog receptor activity
E	0008201	molecular_function	heparin binding
E	0008285	biological_process	negative regulation of cell population proliferation
E	0008544	biological_process	epidermis development
E	0008589	biological_process	regulation of smoothened signaling pathway
E	0009410	biological_process	response to xenobiotic stimulus
E	0009612	biological_process	response to mechanical stimulus
E	0009887	biological_process	animal organ morphogenesis
E	0009953	biological_process	dorsal/ventral pattern formation
E	0009957	biological_process	epidermal cell fate specification
E	0010157	biological_process	response to chlorate
E	0010839	biological_process	negative regulation of keratinocyte proliferation
E	0010875	biological_process	positive regulation of cholesterol efflux
E	0015485	molecular_function	cholesterol binding
E	0016020	cellular_component	membrane
E	0016485	biological_process	protein processing
E	0021522	biological_process	spinal cord motor neuron differentiation
E	0021532	biological_process	neural tube patterning
E	0021904	biological_process	dorsal/ventral neural tube patterning
E	0021997	biological_process	neural plate axis specification
E	0030326	biological_process	embryonic limb morphogenesis
E	0030332	molecular_function	cyclin binding
E	0030496	cellular_component	midbody
E	0030666	cellular_component	endocytic vesicle membrane
E	0030850	biological_process	prostate gland development
E	0030879	biological_process	mammary gland development
E	0032355	biological_process	response to estradiol
E	0032526	biological_process	response to retinoic acid
E	0032880	biological_process	regulation of protein localization
E	0035108	biological_process	limb morphogenesis
E	0035137	biological_process	hindlimb morphogenesis
E	0040008	biological_process	regulation of growth
E	0040015	biological_process	negative regulation of multicellular organism growth
E	0042127	biological_process	regulation of cell population proliferation
E	0042593	biological_process	glucose homeostasis
E	0043279	biological_process	response to alkaloid
E	0043616	biological_process	keratinocyte proliferation
E	0044294	cellular_component	dendritic growth cone
E	0044295	cellular_component	axonal growth cone
E	0044877	molecular_function	protein-containing complex binding
E	0045177	cellular_component	apical part of cell
E	0045211	cellular_component	postsynaptic membrane
E	0045606	biological_process	positive regulation of epidermal cell differentiation
E	0045668	biological_process	negative regulation of osteoblast differentiation
E	0045879	biological_process	negative regulation of smoothened signaling pathway
E	0045892	biological_process	negative regulation of DNA-templated transcription
E	0045893	biological_process	positive regulation of DNA-templated transcription
E	0048471	cellular_component	perinuclear region of cytoplasm
E	0048568	biological_process	embryonic organ development
E	0048745	biological_process	smooth muscle tissue development
E	0050673	biological_process	epithelial cell proliferation
E	0050680	biological_process	negative regulation of epithelial cell proliferation
E	0051782	biological_process	negative regulation of cell division
E	0060037	biological_process	pharyngeal system development
E	0060170	cellular_component	ciliary membrane
E	0060603	biological_process	mammary gland duct morphogenesis
E	0060644	biological_process	mammary gland epithelial cell differentiation
E	0061005	biological_process	cell differentiation involved in kidney development
E	0061053	biological_process	somite development
E	0071397	biological_process	cellular response to cholesterol
E	0071679	biological_process	commissural neuron axon guidance
E	0072089	biological_process	stem cell proliferation
E	0072203	biological_process	cell proliferation involved in metanephros development
E	0072205	biological_process	metanephric collecting duct development
E	0072659	biological_process	protein localization to plasma membrane
E	0097108	molecular_function	hedgehog family protein binding
E	0097421	biological_process	liver regeneration
E	2000647	biological_process	negative regulation of stem cell proliferation
F	0007267	biological_process	cell-cell signaling
F	0007275	biological_process	multicellular organism development

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	956
Details	Transmembrane: {"description":"Helical","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2416
Details	Topological domain: {"description":"Extracellular","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	240
Details	Topological domain: {"description":"Cytoplasmic","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	640
Details	Domain: {"description":"SSD","evidences":[{"source":"PROSITE-ProRule","id":"PRU00199","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	24
Details	Glycosylation: {"description":"N-linked (GlcNAc...) asparagine","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	12
Details	Motif: {"description":"Cardin-Weintraub","evidences":[{"source":"PubMed","id":"23118222","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	14
Details	Binding site: {"evidences":[{"source":"PubMed","id":"20504762","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3MXW","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	6
Details	Binding site: {"evidences":[{"source":"PubMed","id":"10753901","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20504762","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"3M1N","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3MXW","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Lipidation: {"description":"N-palmitoyl cysteine","evidences":[{"source":"PubMed","id":"18534984","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"31875564","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9593755","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Lipidation: {"description":"Cholesterol glycine ester","evidences":[{"source":"UniProtKB","id":"Q62226","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---