6N7K
Cryo-EM structure of tetrameric Ptch1 in complex with ShhNp (form II)
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6.5 |
| Software | RELION,Gctf,PHENIX |
| CTF correction | NONE |
