6MN0
Crystal structure of meta-AAC0038, an environmental aminoglycoside resistance enzyme, H168A mutant in complex with acetyl-CoA
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0008080 | molecular_function | N-acetyltransferase activity |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016746 | molecular_function | acyltransferase activity |
| A | 0046353 | molecular_function | aminoglycoside 3-N-acetyltransferase activity |
| A | 0046677 | biological_process | response to antibiotic |
| B | 0008080 | molecular_function | N-acetyltransferase activity |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016746 | molecular_function | acyltransferase activity |
| B | 0046353 | molecular_function | aminoglycoside 3-N-acetyltransferase activity |
| B | 0046677 | biological_process | response to antibiotic |
| C | 0008080 | molecular_function | N-acetyltransferase activity |
| C | 0016740 | molecular_function | transferase activity |
| C | 0016746 | molecular_function | acyltransferase activity |
| C | 0046353 | molecular_function | aminoglycoside 3-N-acetyltransferase activity |
| C | 0046677 | biological_process | response to antibiotic |
| D | 0008080 | molecular_function | N-acetyltransferase activity |
| D | 0016740 | molecular_function | transferase activity |
| D | 0016746 | molecular_function | acyltransferase activity |
| D | 0046353 | molecular_function | aminoglycoside 3-N-acetyltransferase activity |
| D | 0046677 | biological_process | response to antibiotic |
| E | 0008080 | molecular_function | N-acetyltransferase activity |
| E | 0016740 | molecular_function | transferase activity |
| E | 0016746 | molecular_function | acyltransferase activity |
| E | 0046353 | molecular_function | aminoglycoside 3-N-acetyltransferase activity |
| E | 0046677 | biological_process | response to antibiotic |
| F | 0008080 | molecular_function | N-acetyltransferase activity |
| F | 0016740 | molecular_function | transferase activity |
| F | 0016746 | molecular_function | acyltransferase activity |
| F | 0046353 | molecular_function | aminoglycoside 3-N-acetyltransferase activity |
| F | 0046677 | biological_process | response to antibiotic |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 32 |
| Details | binding site for residue ACO A 301 |
| Chain | Residue |
| A | HIS29 |
| A | PHE97 |
| A | ALA159 |
| A | PRO160 |
| A | THR163 |
| A | THR165 |
| A | HOH416 |
| A | HOH436 |
| A | HOH451 |
| A | HOH453 |
| A | HOH457 |
| A | ALA30 |
| A | HOH465 |
| A | HOH483 |
| A | HOH485 |
| A | HOH507 |
| A | HOH508 |
| A | HOH515 |
| A | HOH527 |
| A | HOH539 |
| A | HOH540 |
| A | HOH544 |
| A | ALA31 |
| B | GLN66 |
| B | ARG93 |
| B | HOH474 |
| A | LEU32 |
| A | ARG33 |
| A | PRO42 |
| A | TYR62 |
| A | ASN95 |
| A | GLY96 |
| site_id | AC2 |
| Number of Residues | 1 |
| Details | binding site for residue CL A 302 |
| Chain | Residue |
| A | ARG257 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 303 |
| Chain | Residue |
| A | TRP65 |
| A | ARG72 |
| A | GLY115 |
| A | TYR184 |
| A | HOH534 |
| A | HOH567 |
| site_id | AC4 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 A 304 |
| Chain | Residue |
| A | GLY158 |
| A | ALA159 |
| A | LEU161 |
| A | ARG231 |
| A | SER238 |
| A | HOH443 |
| site_id | AC5 |
| Number of Residues | 1 |
| Details | binding site for residue SO4 A 305 |
| Chain | Residue |
| A | ARG197 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 A 306 |
| Chain | Residue |
| A | ALA133 |
| A | LEU134 |
| A | ASP135 |
| A | ARG141 |
| site_id | AC7 |
| Number of Residues | 7 |
| Details | binding site for residue PE3 A 307 |
| Chain | Residue |
| A | PRO86 |
| A | ARG110 |
| A | THR129 |
| A | ALA130 |
| A | HOH425 |
| C | ASP135 |
| C | GLU200 |
| site_id | AC8 |
| Number of Residues | 3 |
| Details | binding site for residue PE3 A 308 |
| Chain | Residue |
| A | TRP258 |
| A | THR261 |
| A | HOH440 |
| site_id | AC9 |
| Number of Residues | 4 |
| Details | binding site for residue PE3 A 309 |
| Chain | Residue |
| A | LEU224 |
| A | ALA225 |
| A | HOH478 |
| A | HOH481 |
| site_id | AD1 |
| Number of Residues | 29 |
| Details | binding site for residue ACO B 301 |
| Chain | Residue |
| A | GLN66 |
| A | ARG93 |
| B | HIS29 |
| B | ALA30 |
| B | ALA31 |
| B | LEU32 |
| B | ARG33 |
| B | PRO42 |
| B | ASN95 |
| B | GLY96 |
| B | PHE97 |
| B | ALA159 |
| B | PRO160 |
| B | THR163 |
| B | THR165 |
| B | HOH412 |
| B | HOH413 |
| B | HOH433 |
| B | HOH468 |
| B | HOH475 |
| B | HOH478 |
| B | HOH489 |
| B | HOH496 |
| B | HOH499 |
| B | HOH500 |
| B | HOH511 |
| B | HOH537 |
| B | HOH632 |
| B | HOH661 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 B 302 |
| Chain | Residue |
| B | ALA133 |
| B | LEU134 |
| B | ASP135 |
| B | ARG141 |
| site_id | AD3 |
| Number of Residues | 2 |
| Details | binding site for residue SO4 B 303 |
| Chain | Residue |
| B | ARG257 |
| B | ARG260 |
| site_id | AD4 |
| Number of Residues | 5 |
| Details | binding site for residue PE3 B 304 |
| Chain | Residue |
| B | ASP191 |
| B | HOH401 |
| C | ARG257 |
| C | TRP258 |
| C | THR261 |
| site_id | AD5 |
| Number of Residues | 5 |
| Details | binding site for residue PE3 B 305 |
| Chain | Residue |
| B | PRO86 |
| B | ARG110 |
| B | GLU126 |
| B | THR129 |
| D | GLU200 |
| site_id | AD6 |
| Number of Residues | 30 |
| Details | binding site for residue ACO C 301 |
| Chain | Residue |
| C | HIS29 |
| C | ALA30 |
| C | ALA31 |
| C | LEU32 |
| C | ARG33 |
| C | PRO42 |
| C | TYR62 |
| C | ASN95 |
| C | GLY96 |
| C | PHE97 |
| C | ALA159 |
| C | PRO160 |
| C | ASP162 |
| C | THR163 |
| C | THR165 |
| C | HOH415 |
| C | HOH443 |
| C | HOH447 |
| C | HOH454 |
| C | HOH468 |
| C | HOH473 |
| C | HOH477 |
| C | HOH495 |
| C | HOH521 |
| C | HOH529 |
| C | HOH568 |
| C | HOH624 |
| C | HOH660 |
| F | GLN66 |
| F | ARG93 |
| site_id | AD7 |
| Number of Residues | 2 |
| Details | binding site for residue CL C 302 |
| Chain | Residue |
| C | SER9 |
| F | HIS80 |
| site_id | AD8 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 C 303 |
| Chain | Residue |
| C | ARG197 |
| C | HOH405 |
| C | HOH592 |
| site_id | AD9 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 C 304 |
| Chain | Residue |
| C | ALA112 |
| C | ALA130 |
| C | ASP131 |
| C | HOH420 |
| C | HOH478 |
| site_id | AE1 |
| Number of Residues | 1 |
| Details | binding site for residue SO4 C 305 |
| Chain | Residue |
| C | ARG228 |
| site_id | AE2 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 C 306 |
| Chain | Residue |
| C | LYS230 |
| C | ARG231 |
| C | HOH458 |
| site_id | AE3 |
| Number of Residues | 8 |
| Details | binding site for residue PE3 C 307 |
| Chain | Residue |
| C | ARG183 |
| C | HOH504 |
| D | PRO177 |
| D | TRP258 |
| D | GLY259 |
| D | THR261 |
| D | ALA262 |
| D | HOH565 |
| site_id | AE4 |
| Number of Residues | 26 |
| Details | binding site for residue ACO D 301 |
| Chain | Residue |
| D | HIS29 |
| D | ALA30 |
| D | ALA31 |
| D | LEU32 |
| D | ARG33 |
| D | PRO42 |
| D | ASN95 |
| D | GLY96 |
| D | PHE97 |
| D | ALA159 |
| D | PRO160 |
| D | ASP162 |
| D | THR163 |
| D | THR165 |
| D | HOH428 |
| D | HOH441 |
| D | HOH448 |
| D | HOH462 |
| D | HOH470 |
| D | HOH484 |
| D | HOH503 |
| D | HOH510 |
| D | HOH534 |
| D | HOH600 |
| E | GLN66 |
| E | ARG93 |
| site_id | AE5 |
| Number of Residues | 3 |
| Details | binding site for residue GOL D 302 |
| Chain | Residue |
| D | GLU124 |
| D | TRP127 |
| D | HOH432 |
| site_id | AE6 |
| Number of Residues | 7 |
| Details | binding site for residue GOL D 303 |
| Chain | Residue |
| B | HOH425 |
| D | PRO140 |
| D | ARG141 |
| D | LYS146 |
| D | GLU149 |
| D | HOH406 |
| D | HOH592 |
| site_id | AE7 |
| Number of Residues | 7 |
| Details | binding site for residue GOL D 304 |
| Chain | Residue |
| D | ASP64 |
| D | TRP65 |
| D | GLY115 |
| D | TYR184 |
| D | HOH402 |
| D | HOH426 |
| D | HOH500 |
| site_id | AE8 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 D 305 |
| Chain | Residue |
| D | ASP131 |
| D | HOH407 |
| D | HOH456 |
| site_id | AE9 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 D 306 |
| Chain | Residue |
| D | ARG78 |
| D | TRP196 |
| D | HOH494 |
| site_id | AF1 |
| Number of Residues | 30 |
| Details | binding site for residue ACO E 301 |
| Chain | Residue |
| D | GLN66 |
| D | ARG93 |
| E | HIS29 |
| E | ALA30 |
| E | ALA31 |
| E | LEU32 |
| E | ARG33 |
| E | PRO42 |
| E | TYR62 |
| E | ASN95 |
| E | GLY96 |
| E | PHE97 |
| E | ALA159 |
| E | PRO160 |
| E | THR163 |
| E | THR165 |
| E | HOH408 |
| E | HOH412 |
| E | HOH425 |
| E | HOH430 |
| E | HOH445 |
| E | HOH451 |
| E | HOH452 |
| E | HOH480 |
| E | HOH494 |
| E | HOH502 |
| E | HOH525 |
| E | HOH535 |
| E | HOH554 |
| E | HOH604 |
| site_id | AF2 |
| Number of Residues | 4 |
| Details | binding site for residue GOL E 302 |
| Chain | Residue |
| E | ARG110 |
| E | THR129 |
| E | ALA130 |
| E | SO4303 |
| site_id | AF3 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 E 303 |
| Chain | Residue |
| E | ALA112 |
| E | ALA130 |
| E | ASP131 |
| E | GOL302 |
| E | HOH432 |
| E | HOH457 |
| site_id | AF4 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 E 304 |
| Chain | Residue |
| E | ARG8 |
| E | HOH424 |
| E | HOH437 |
| site_id | AF5 |
| Number of Residues | 2 |
| Details | binding site for residue SO4 E 305 |
| Chain | Residue |
| E | ARG197 |
| E | SO4308 |
| site_id | AF6 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 E 306 |
| Chain | Residue |
| E | PRO140 |
| E | ARG141 |
| E | HOH477 |
| E | HOH519 |
| site_id | AF7 |
| Number of Residues | 3 |
| Details | binding site for residue SO4 E 307 |
| Chain | Residue |
| E | TRP254 |
| E | ARG257 |
| E | HOH402 |
| site_id | AF8 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 E 308 |
| Chain | Residue |
| E | ALA133 |
| E | LEU134 |
| E | ASP135 |
| E | ARG141 |
| E | SO4305 |
| site_id | AF9 |
| Number of Residues | 5 |
| Details | binding site for residue PE3 E 309 |
| Chain | Residue |
| E | ASP64 |
| E | ILE92 |
| E | GLU201 |
| E | HOH409 |
| E | HOH518 |
| site_id | AG1 |
| Number of Residues | 29 |
| Details | binding site for residue ACO F 301 |
| Chain | Residue |
| C | GLN66 |
| C | ARG93 |
| F | HIS29 |
| F | ALA30 |
| F | ALA31 |
| F | LEU32 |
| F | ARG33 |
| F | PRO42 |
| F | TYR62 |
| F | ASN95 |
| F | GLY96 |
| F | PHE97 |
| F | ALA159 |
| F | PRO160 |
| F | ASP162 |
| F | THR163 |
| F | THR165 |
| F | HOH415 |
| F | HOH417 |
| F | HOH433 |
| F | HOH436 |
| F | HOH445 |
| F | HOH449 |
| F | HOH468 |
| F | HOH494 |
| F | HOH496 |
| F | HOH499 |
| F | HOH515 |
| F | HOH543 |
| site_id | AG2 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 F 303 |
| Chain | Residue |
| F | ALA133 |
| F | LEU134 |
| F | ASP135 |
| F | ARG141 |
| F | HOH435 |
| F | HOH455 |
| site_id | AG3 |
| Number of Residues | 2 |
| Details | binding site for residue SO4 F 304 |
| Chain | Residue |
| F | ARG8 |
| F | HOH427 |
| site_id | AG4 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 F 305 |
| Chain | Residue |
| F | ASP131 |
| F | HIS132 |
| F | HOH410 |
| F | HOH458 |
| F | HOH474 |
| F | HOH492 |
| site_id | AG5 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 F 306 |
| Chain | Residue |
| D | SER3 |
| F | ARG78 |
| F | TRP196 |
| F | HOH424 |
| site_id | AG6 |
| Number of Residues | 2 |
| Details | binding site for residue PE3 F 307 |
| Chain | Residue |
| F | PRO55 |
| F | ALA56 |
