Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6FL5

Structure of human SHMT1-H135N-R137A-E168N mutant at 3.6 Ang. resolution

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
A	0004372	molecular_function	glycine hydroxymethyltransferase activity
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005829	cellular_component	cytosol
A	0006231	biological_process	dTMP biosynthetic process
A	0006544	biological_process	glycine metabolic process
A	0006563	biological_process	L-serine metabolic process
A	0006565	biological_process	L-serine catabolic process
A	0006730	biological_process	one-carbon metabolic process
A	0009113	biological_process	purine nucleobase biosynthetic process
A	0016740	molecular_function	transferase activity
A	0016832	molecular_function	aldehyde-lyase activity
A	0017148	biological_process	negative regulation of translation
A	0019264	biological_process	glycine biosynthetic process from serine
A	0030170	molecular_function	pyridoxal phosphate binding
A	0035999	biological_process	tetrahydrofolate interconversion
A	0036094	molecular_function	small molecule binding
A	0042802	molecular_function	identical protein binding
A	0042803	molecular_function	protein homodimerization activity
A	0045329	biological_process	carnitine biosynthetic process
A	0046653	biological_process	tetrahydrofolate metabolic process
A	0046655	biological_process	folic acid metabolic process
A	0048027	molecular_function	mRNA 5'-UTR binding
A	0051289	biological_process	protein homotetramerization
A	0070062	cellular_component	extracellular exosome
A	0070905	molecular_function	serine binding
A	1904482	biological_process	cellular response to tetrahydrofolate
A	1990830	biological_process	cellular response to leukemia inhibitory factor
D	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
D	0004372	molecular_function	glycine hydroxymethyltransferase activity
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005829	cellular_component	cytosol
D	0006231	biological_process	dTMP biosynthetic process
D	0006544	biological_process	glycine metabolic process
D	0006563	biological_process	L-serine metabolic process
D	0006565	biological_process	L-serine catabolic process
D	0006730	biological_process	one-carbon metabolic process
D	0009113	biological_process	purine nucleobase biosynthetic process
D	0016740	molecular_function	transferase activity
D	0016832	molecular_function	aldehyde-lyase activity
D	0017148	biological_process	negative regulation of translation
D	0019264	biological_process	glycine biosynthetic process from serine
D	0030170	molecular_function	pyridoxal phosphate binding
D	0035999	biological_process	tetrahydrofolate interconversion
D	0036094	molecular_function	small molecule binding
D	0042802	molecular_function	identical protein binding
D	0042803	molecular_function	protein homodimerization activity
D	0045329	biological_process	carnitine biosynthetic process
D	0046653	biological_process	tetrahydrofolate metabolic process
D	0046655	biological_process	folic acid metabolic process
D	0048027	molecular_function	mRNA 5'-UTR binding
D	0051289	biological_process	protein homotetramerization
D	0070062	cellular_component	extracellular exosome
D	0070905	molecular_function	serine binding
D	1904482	biological_process	cellular response to tetrahydrofolate
D	1990830	biological_process	cellular response to leukemia inhibitory factor
G	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
G	0004372	molecular_function	glycine hydroxymethyltransferase activity
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005739	cellular_component	mitochondrion
G	0005829	cellular_component	cytosol
G	0006231	biological_process	dTMP biosynthetic process
G	0006544	biological_process	glycine metabolic process
G	0006563	biological_process	L-serine metabolic process
G	0006565	biological_process	L-serine catabolic process
G	0006730	biological_process	one-carbon metabolic process
G	0009113	biological_process	purine nucleobase biosynthetic process
G	0016740	molecular_function	transferase activity
G	0016832	molecular_function	aldehyde-lyase activity
G	0017148	biological_process	negative regulation of translation
G	0019264	biological_process	glycine biosynthetic process from serine
G	0030170	molecular_function	pyridoxal phosphate binding
G	0035999	biological_process	tetrahydrofolate interconversion
G	0036094	molecular_function	small molecule binding
G	0042802	molecular_function	identical protein binding
G	0042803	molecular_function	protein homodimerization activity
G	0045329	biological_process	carnitine biosynthetic process
G	0046653	biological_process	tetrahydrofolate metabolic process
G	0046655	biological_process	folic acid metabolic process
G	0048027	molecular_function	mRNA 5'-UTR binding
G	0051289	biological_process	protein homotetramerization
G	0070062	cellular_component	extracellular exosome
G	0070905	molecular_function	serine binding
G	1904482	biological_process	cellular response to tetrahydrofolate
G	1990830	biological_process	cellular response to leukemia inhibitory factor
J	0000900	molecular_function	mRNA regulatory element binding translation repressor activity
J	0004372	molecular_function	glycine hydroxymethyltransferase activity
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005737	cellular_component	cytoplasm
J	0005739	cellular_component	mitochondrion
J	0005829	cellular_component	cytosol
J	0006231	biological_process	dTMP biosynthetic process
J	0006544	biological_process	glycine metabolic process
J	0006563	biological_process	L-serine metabolic process
J	0006565	biological_process	L-serine catabolic process
J	0006730	biological_process	one-carbon metabolic process
J	0009113	biological_process	purine nucleobase biosynthetic process
J	0016740	molecular_function	transferase activity
J	0016832	molecular_function	aldehyde-lyase activity
J	0017148	biological_process	negative regulation of translation
J	0019264	biological_process	glycine biosynthetic process from serine
J	0030170	molecular_function	pyridoxal phosphate binding
J	0035999	biological_process	tetrahydrofolate interconversion
J	0036094	molecular_function	small molecule binding
J	0042802	molecular_function	identical protein binding
J	0042803	molecular_function	protein homodimerization activity
J	0045329	biological_process	carnitine biosynthetic process
J	0046653	biological_process	tetrahydrofolate metabolic process
J	0046655	biological_process	folic acid metabolic process
J	0048027	molecular_function	mRNA 5'-UTR binding
J	0051289	biological_process	protein homotetramerization
J	0070062	cellular_component	extracellular exosome
J	0070905	molecular_function	serine binding
J	1904482	biological_process	cellular response to tetrahydrofolate
J	1990830	biological_process	cellular response to leukemia inhibitory factor

Functional Information from PDB Data

site_id	AC1
Number of Residues	16
Details	binding site for residue PLP A 501

Chain	Residue
A	SER119
A	HIS231
A	HIS256
A	LYS257
D	TYR73
D	TYR118
D	GLY302
D	GLY303
A	GLY120
A	SER121
A	HIS148
A	HIS151
A	THR202
A	SER203
A	ASP228
A	ALA230

site_id	AC2
Number of Residues	2
Details	binding site for residue CL A 502

Chain	Residue
A	SER203
D	TYR83

site_id	AC3
Number of Residues	2
Details	binding site for residue CL A 503

Chain	Residue
A	TYR83
D	SER203

site_id	AC4
Number of Residues	3
Details	binding site for residue CL G 502

Chain	Residue
G	TYR83
J	SER53
J	SER203

site_id	AC5
Number of Residues	3
Details	binding site for residue CL J 502

Chain	Residue
G	SER53
G	SER203
J	TYR83

site_id	AC6
Number of Residues	18
Details	binding site for Di-peptide PLP D 500 and LYS D 257

Chain	Residue
A	TYR73
A	GLY302
A	GLY303
D	SER53
D	ASN55
D	SER119
D	GLY120
D	SER121
D	HIS148
D	SER203
D	ASP228
D	ALA230
D	HIS231
D	THR254
D	HIS256
D	THR258
D	LEU259
D	ARG260

site_id	AC7
Number of Residues	18
Details	binding site for Di-peptide PLP G 501 and LYS G 257

Chain	Residue
G	SER53
G	ASN55
G	SER119
G	GLY120
G	SER121
G	HIS148
G	HIS151
G	SER203
G	ASP228
G	ALA230
G	HIS231
G	THR254
G	HIS256
G	THR258
G	LEU259
G	ARG260
J	TYR73
J	GLY303

site_id	AC8
Number of Residues	17
Details	binding site for Di-peptide PLP J 501 and LYS J 257

Chain	Residue
G	TYR73
G	GLY303
J	SER53
J	ASN55
J	SER119
J	GLY120
J	SER121
J	HIS148
J	SER203
J	ASP228
J	ALA230
J	HIS231
J	THR254
J	HIS256
J	THR258
J	LEU259
J	ARG260

Functional Information from PROSITE/UniProt

site_id	PS00096
Number of Residues	17
Details	SHMT Serine hydroxymethyltransferase pyridoxal-phosphate attachment site. HVvTTTTHKTLrGCRAG

Chain	Residue	Details
A	HIS249-GLY265

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	MOD_RES: N6-(pyridoxal phosphate)lysine => ECO:0000269\|PubMed:9753690, ECO:0007744\|PDB:1BJ4

Chain	Residue	Details
A	LYS257
D	LYS257
G	LYS257
J	LYS257

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)