6FD6
Crystal Structure of Human APRT-Tyr105Phe variant in complex with Adenine, PRPP and Mg2+, 30 days post crystallization (with AMP and PPi products fully generated)
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0002055 | molecular_function | adenine binding |
| A | 0003999 | molecular_function | adenine phosphoribosyltransferase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005576 | cellular_component | extracellular region |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006166 | biological_process | purine ribonucleoside salvage |
| A | 0006168 | biological_process | adenine salvage |
| A | 0007625 | biological_process | grooming behavior |
| A | 0016208 | molecular_function | AMP binding |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016757 | molecular_function | glycosyltransferase activity |
| A | 0032263 | biological_process | GMP salvage |
| A | 0032264 | biological_process | IMP salvage |
| A | 0034774 | cellular_component | secretory granule lumen |
| A | 0044209 | biological_process | AMP salvage |
| A | 0046083 | biological_process | adenine metabolic process |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 1901363 | molecular_function | heterocyclic compound binding |
| B | 0002055 | molecular_function | adenine binding |
| B | 0003999 | molecular_function | adenine phosphoribosyltransferase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005576 | cellular_component | extracellular region |
| B | 0005654 | cellular_component | nucleoplasm |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0006166 | biological_process | purine ribonucleoside salvage |
| B | 0006168 | biological_process | adenine salvage |
| B | 0007625 | biological_process | grooming behavior |
| B | 0016208 | molecular_function | AMP binding |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016757 | molecular_function | glycosyltransferase activity |
| B | 0032263 | biological_process | GMP salvage |
| B | 0032264 | biological_process | IMP salvage |
| B | 0034774 | cellular_component | secretory granule lumen |
| B | 0044209 | biological_process | AMP salvage |
| B | 0046083 | biological_process | adenine metabolic process |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 1901363 | molecular_function | heterocyclic compound binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 19 |
| Details | binding site for residue AMP A 200 |
| Chain | Residue |
| A | VAL25 |
| A | GLY133 |
| A | GLY134 |
| A | THR135 |
| A | LEU159 |
| A | PPV201 |
| A | HOH301 |
| A | HOH311 |
| A | HOH312 |
| A | HOH340 |
| A | HOH349 |
| A | PHE26 |
| A | ARG27 |
| A | ARG67 |
| A | ASP127 |
| A | ASP128 |
| A | LEU129 |
| A | ALA131 |
| A | THR132 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue PPV A 201 |
| Chain | Residue |
| A | SER66 |
| A | ARG67 |
| A | AMP200 |
| A | HOH315 |
| B | ARG87 |
| B | LYS91 |
| site_id | AC3 |
| Number of Residues | 20 |
| Details | binding site for residue AMP B 201 |
| Chain | Residue |
| B | VAL25 |
| B | PHE26 |
| B | ARG27 |
| B | ARG67 |
| B | ASP127 |
| B | ASP128 |
| B | LEU129 |
| B | ALA131 |
| B | THR132 |
| B | GLY133 |
| B | GLY134 |
| B | THR135 |
| B | LEU159 |
| B | MG202 |
| B | PPV203 |
| B | HOH323 |
| B | HOH333 |
| B | HOH336 |
| B | HOH340 |
| B | HOH343 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue MG B 202 |
| Chain | Residue |
| B | AMP201 |
| B | PPV203 |
| B | HOH304 |
| B | HOH333 |
| site_id | AC5 |
| Number of Residues | 11 |
| Details | binding site for residue PPV B 203 |
| Chain | Residue |
| A | ARG87 |
| A | LYS91 |
| B | SER66 |
| B | ARG67 |
| B | LYS88 |
| B | AMP201 |
| B | MG202 |
| B | HOH303 |
| B | HOH304 |
| B | HOH333 |
| B | HOH344 |
Functional Information from PROSITE/UniProt
| site_id | PS00103 |
| Number of Residues | 13 |
| Details | PUR_PYR_PR_TRANSFER Purine/pyrimidine phosphoribosyl transferases signature. VVVVDDLLATGgT |
| Chain | Residue | Details |
| A | VAL123-THR135 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19369195","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19369195","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"19369195","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19369195","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"19369195","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
