6EPD

Substrate processing state 26S proteasome (SPS1)

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0000502	cellular_component	proteasome complex
1	0004175	molecular_function	endopeptidase activity
1	0004298	molecular_function	threonine-type endopeptidase activity
1	0005634	cellular_component	nucleus
1	0005737	cellular_component	cytoplasm
1	0005829	cellular_component	cytosol
1	0005839	cellular_component	proteasome core complex
1	0006508	biological_process	proteolysis
1	0008233	molecular_function	peptidase activity
1	0016787	molecular_function	hydrolase activity
1	0019774	cellular_component	proteasome core complex, beta-subunit complex
1	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
1	0051603	biological_process	proteolysis involved in protein catabolic process
2	0000502	cellular_component	proteasome complex
2	0004175	molecular_function	endopeptidase activity
2	0004298	molecular_function	threonine-type endopeptidase activity
2	0005634	cellular_component	nucleus
2	0005737	cellular_component	cytoplasm
2	0005829	cellular_component	cytosol
2	0005839	cellular_component	proteasome core complex
2	0006508	biological_process	proteolysis
2	0008233	molecular_function	peptidase activity
2	0016787	molecular_function	hydrolase activity
2	0019774	cellular_component	proteasome core complex, beta-subunit complex
2	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
2	0051603	biological_process	proteolysis involved in protein catabolic process
3	0000502	cellular_component	proteasome complex
3	0005634	cellular_component	nucleus
3	0005737	cellular_component	cytoplasm
3	0005829	cellular_component	cytosol
3	0005839	cellular_component	proteasome core complex
3	0019774	cellular_component	proteasome core complex, beta-subunit complex
3	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
3	0051603	biological_process	proteolysis involved in protein catabolic process
4	0000502	cellular_component	proteasome complex
4	0005634	cellular_component	nucleus
4	0005654	cellular_component	nucleoplasm
4	0005737	cellular_component	cytoplasm
4	0005829	cellular_component	cytosol
4	0005839	cellular_component	proteasome core complex
4	0010243	biological_process	response to organonitrogen compound
4	0010498	biological_process	proteasomal protein catabolic process
4	0014070	biological_process	response to organic cyclic compound
4	0019774	cellular_component	proteasome core complex, beta-subunit complex
4	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
4	0051603	biological_process	proteolysis involved in protein catabolic process
5	0000502	cellular_component	proteasome complex
5	0004175	molecular_function	endopeptidase activity
5	0004298	molecular_function	threonine-type endopeptidase activity
5	0005634	cellular_component	nucleus
5	0005737	cellular_component	cytoplasm
5	0005829	cellular_component	cytosol
5	0005839	cellular_component	proteasome core complex
5	0006508	biological_process	proteolysis
5	0006979	biological_process	response to oxidative stress
5	0008233	molecular_function	peptidase activity
5	0016787	molecular_function	hydrolase activity
5	0019774	cellular_component	proteasome core complex, beta-subunit complex
5	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
5	0051603	biological_process	proteolysis involved in protein catabolic process
6	0000502	cellular_component	proteasome complex
6	0005634	cellular_component	nucleus
6	0005737	cellular_component	cytoplasm
6	0005839	cellular_component	proteasome core complex
6	0019774	cellular_component	proteasome core complex, beta-subunit complex
6	0051603	biological_process	proteolysis involved in protein catabolic process
7	0000502	cellular_component	proteasome complex
7	0001530	molecular_function	lipopolysaccharide binding
7	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
7	0005634	cellular_component	nucleus
7	0005737	cellular_component	cytoplasm
7	0005829	cellular_component	cytosol
7	0005839	cellular_component	proteasome core complex
7	0019774	cellular_component	proteasome core complex, beta-subunit complex
7	0036064	cellular_component	ciliary basal body
7	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
7	0051603	biological_process	proteolysis involved in protein catabolic process
A	0000502	cellular_component	proteasome complex
A	0000932	cellular_component	P-body
A	0003723	molecular_function	RNA binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005839	cellular_component	proteasome core complex
A	0005840	cellular_component	ribosome
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0010498	biological_process	proteasomal protein catabolic process
A	0016363	cellular_component	nuclear matrix
A	0019773	cellular_component	proteasome core complex, alpha-subunit complex
A	0030016	cellular_component	myofibril
A	0030017	cellular_component	sarcomere
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0051059	molecular_function	NF-kappaB binding
A	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
A	0051603	biological_process	proteolysis involved in protein catabolic process
B	0000502	cellular_component	proteasome complex
B	0000932	cellular_component	P-body
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005839	cellular_component	proteasome core complex
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0009615	biological_process	response to virus
B	0010498	biological_process	proteasomal protein catabolic process
B	0019773	cellular_component	proteasome core complex, alpha-subunit complex
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0051603	biological_process	proteolysis involved in protein catabolic process
C	0000502	cellular_component	proteasome complex
C	0000932	cellular_component	P-body
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005839	cellular_component	proteasome core complex
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0010498	biological_process	proteasomal protein catabolic process
C	0019773	cellular_component	proteasome core complex, alpha-subunit complex
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0043231	cellular_component	intracellular membrane-bounded organelle
C	0051603	biological_process	proteolysis involved in protein catabolic process
D	0000502	cellular_component	proteasome complex
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005839	cellular_component	proteasome core complex
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0010498	biological_process	proteasomal protein catabolic process
D	0019773	cellular_component	proteasome core complex, alpha-subunit complex
D	0042802	molecular_function	identical protein binding
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0051603	biological_process	proteolysis involved in protein catabolic process
D	0098794	cellular_component	postsynapse
E	0000502	cellular_component	proteasome complex
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005839	cellular_component	proteasome core complex
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0010498	biological_process	proteasomal protein catabolic process
E	0019773	cellular_component	proteasome core complex, alpha-subunit complex
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0051603	biological_process	proteolysis involved in protein catabolic process
F	0000502	cellular_component	proteasome complex
F	0001530	molecular_function	lipopolysaccharide binding
F	0002376	biological_process	immune system process
F	0002862	biological_process	negative regulation of inflammatory response to antigenic stimulus
F	0005634	cellular_component	nucleus
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005813	cellular_component	centrosome
F	0005839	cellular_component	proteasome core complex
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0010498	biological_process	proteasomal protein catabolic process
F	0019773	cellular_component	proteasome core complex, alpha-subunit complex
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0051603	biological_process	proteolysis involved in protein catabolic process
G	0000502	cellular_component	proteasome complex
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005839	cellular_component	proteasome core complex
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0010498	biological_process	proteasomal protein catabolic process
G	0019773	cellular_component	proteasome core complex, alpha-subunit complex
G	0031625	molecular_function	ubiquitin protein ligase binding
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0045202	cellular_component	synapse
G	0051603	biological_process	proteolysis involved in protein catabolic process
H	0000166	molecular_function	nucleotide binding
H	0000502	cellular_component	proteasome complex
H	0000932	cellular_component	P-body
H	0005524	molecular_function	ATP binding
H	0005737	cellular_component	cytoplasm
H	0006511	biological_process	ubiquitin-dependent protein catabolic process
H	0008540	cellular_component	proteasome regulatory particle, base subcomplex
H	0016887	molecular_function	ATP hydrolysis activity
H	0017025	molecular_function	TBP-class protein binding
H	0022624	cellular_component	proteasome accessory complex
H	0036402	molecular_function	proteasome-activating activity
H	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
H	0043197	cellular_component	dendritic spine
H	0140296	molecular_function	general transcription initiation factor binding
H	1901800	biological_process	positive regulation of proteasomal protein catabolic process
I	0000166	molecular_function	nucleotide binding
I	0000502	cellular_component	proteasome complex
I	0005524	molecular_function	ATP binding
I	0005634	cellular_component	nucleus
I	0005737	cellular_component	cytoplasm
I	0005838	cellular_component	proteasome regulatory particle
I	0008150	biological_process	biological_process
I	0008540	cellular_component	proteasome regulatory particle, base subcomplex
I	0016020	cellular_component	membrane
I	0016887	molecular_function	ATP hydrolysis activity
I	0017025	molecular_function	TBP-class protein binding
I	0022624	cellular_component	proteasome accessory complex
I	0036402	molecular_function	proteasome-activating activity
I	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
I	1901800	biological_process	positive regulation of proteasomal protein catabolic process
J	0000166	molecular_function	nucleotide binding
J	0000502	cellular_component	proteasome complex
J	0005102	molecular_function	signaling receptor binding
J	0005524	molecular_function	ATP binding
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005838	cellular_component	proteasome regulatory particle
J	0006357	biological_process	regulation of transcription by RNA polymerase II
J	0008540	cellular_component	proteasome regulatory particle, base subcomplex
J	0016234	cellular_component	inclusion body
J	0016887	molecular_function	ATP hydrolysis activity
J	0017025	molecular_function	TBP-class protein binding
J	0022624	cellular_component	proteasome accessory complex
J	0031410	cellular_component	cytoplasmic vesicle
J	0031531	molecular_function	thyrotropin-releasing hormone receptor binding
J	0031595	cellular_component	nuclear proteasome complex
J	0031597	cellular_component	cytosolic proteasome complex
J	0036402	molecular_function	proteasome-activating activity
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0045892	biological_process	negative regulation of DNA-templated transcription
J	0050804	biological_process	modulation of chemical synaptic transmission
J	0090261	biological_process	positive regulation of inclusion body assembly
J	0098794	cellular_component	postsynapse
J	0099576	biological_process	regulation of protein catabolic process at postsynapse, modulating synaptic transmission
J	0140296	molecular_function	general transcription initiation factor binding
J	0140297	molecular_function	DNA-binding transcription factor binding
J	1901800	biological_process	positive regulation of proteasomal protein catabolic process
K	0000166	molecular_function	nucleotide binding
K	0000502	cellular_component	proteasome complex
K	0001824	biological_process	blastocyst development
K	0005524	molecular_function	ATP binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0008540	cellular_component	proteasome regulatory particle, base subcomplex
K	0016234	cellular_component	inclusion body
K	0016887	molecular_function	ATP hydrolysis activity
K	0022624	cellular_component	proteasome accessory complex
K	0031597	cellular_component	cytosolic proteasome complex
K	0036402	molecular_function	proteasome-activating activity
K	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
K	0045202	cellular_component	synapse
K	1901800	biological_process	positive regulation of proteasomal protein catabolic process
L	0000166	molecular_function	nucleotide binding
L	0000502	cellular_component	proteasome complex
L	0005524	molecular_function	ATP binding
L	0005634	cellular_component	nucleus
L	0008540	cellular_component	proteasome regulatory particle, base subcomplex
L	0016234	cellular_component	inclusion body
L	0016887	molecular_function	ATP hydrolysis activity
L	0022624	cellular_component	proteasome accessory complex
L	0031597	cellular_component	cytosolic proteasome complex
L	0036402	molecular_function	proteasome-activating activity
L	0036503	biological_process	ERAD pathway
L	0042802	molecular_function	identical protein binding
L	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
L	0045899	biological_process	positive regulation of RNA polymerase II transcription preinitiation complex assembly
L	0090261	biological_process	positive regulation of inclusion body assembly
L	1901800	biological_process	positive regulation of proteasomal protein catabolic process
M	0000166	molecular_function	nucleotide binding
M	0000502	cellular_component	proteasome complex
M	0000932	cellular_component	P-body
M	0001824	biological_process	blastocyst development
M	0005515	molecular_function	protein binding
M	0005524	molecular_function	ATP binding
M	0005634	cellular_component	nucleus
M	0005737	cellular_component	cytoplasm
M	0008540	cellular_component	proteasome regulatory particle, base subcomplex
M	0016887	molecular_function	ATP hydrolysis activity
M	0022624	cellular_component	proteasome accessory complex
M	0036402	molecular_function	proteasome-activating activity
M	0042802	molecular_function	identical protein binding
M	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
M	0043921	biological_process	modulation by host of viral transcription
M	0045944	biological_process	positive regulation of transcription by RNA polymerase II
M	0048471	cellular_component	perinuclear region of cytoplasm
M	1901800	biological_process	positive regulation of proteasomal protein catabolic process
N	0000502	cellular_component	proteasome complex
N	0005634	cellular_component	nucleus
N	0008540	cellular_component	proteasome regulatory particle, base subcomplex
N	0022624	cellular_component	proteasome accessory complex
N	0030234	molecular_function	enzyme regulator activity
N	0031625	molecular_function	ubiquitin protein ligase binding
N	0034515	cellular_component	proteasome storage granule
N	0042176	biological_process	regulation of protein catabolic process
N	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
O	0000502	cellular_component	proteasome complex
O	0005198	molecular_function	structural molecule activity
O	0005634	cellular_component	nucleus
O	0005829	cellular_component	cytosol
O	0005838	cellular_component	proteasome regulatory particle
O	0006511	biological_process	ubiquitin-dependent protein catabolic process
O	0007127	biological_process	meiosis I
O	0008541	cellular_component	proteasome regulatory particle, lid subcomplex
O	0022624	cellular_component	proteasome accessory complex
P	0000502	cellular_component	proteasome complex
P	0005737	cellular_component	cytoplasm
P	0005838	cellular_component	proteasome regulatory particle
P	0008541	cellular_component	proteasome regulatory particle, lid subcomplex
P	0022624	cellular_component	proteasome accessory complex
Q	0000502	cellular_component	proteasome complex
Q	0005198	molecular_function	structural molecule activity
Q	0005634	cellular_component	nucleus
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005838	cellular_component	proteasome regulatory particle
Q	0006511	biological_process	ubiquitin-dependent protein catabolic process
Q	0008541	cellular_component	proteasome regulatory particle, lid subcomplex
Q	0022624	cellular_component	proteasome accessory complex
Q	0043248	biological_process	proteasome assembly
Q	0048863	biological_process	stem cell differentiation
R	0000502	cellular_component	proteasome complex
R	0005838	cellular_component	proteasome regulatory particle
R	0022624	cellular_component	proteasome accessory complex
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
S	0000502	cellular_component	proteasome complex
S	0005838	cellular_component	proteasome regulatory particle
S	0006511	biological_process	ubiquitin-dependent protein catabolic process
S	0008541	cellular_component	proteasome regulatory particle, lid subcomplex
S	0022624	cellular_component	proteasome accessory complex
S	0030234	molecular_function	enzyme regulator activity
S	0042176	biological_process	regulation of protein catabolic process
T	0000502	cellular_component	proteasome complex
T	0005634	cellular_component	nucleus
T	0005829	cellular_component	cytosol
T	0005838	cellular_component	proteasome regulatory particle
T	0006508	biological_process	proteolysis
T	0008541	cellular_component	proteasome regulatory particle, lid subcomplex
T	0022624	cellular_component	proteasome accessory complex
T	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
U	0005838	cellular_component	proteasome regulatory particle
U	0008233	molecular_function	peptidase activity
U	0008237	molecular_function	metallopeptidase activity
V	0000502	cellular_component	proteasome complex
V	0000724	biological_process	double-strand break repair via homologous recombination
V	0005634	cellular_component	nucleus
V	0006303	biological_process	double-strand break repair via nonhomologous end joining
V	0008233	molecular_function	peptidase activity
V	0008237	molecular_function	metallopeptidase activity
V	0008541	cellular_component	proteasome regulatory particle, lid subcomplex
V	0022624	cellular_component	proteasome accessory complex
V	0031597	cellular_component	cytosolic proteasome complex
V	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
V	0045471	biological_process	response to ethanol
V	0061133	molecular_function	endopeptidase activator activity
V	0061136	biological_process	regulation of proteasomal protein catabolic process
V	0070628	molecular_function	proteasome binding
V	0140492	molecular_function	metal-dependent deubiquitinase activity
W	0000502	cellular_component	proteasome complex
W	0005515	molecular_function	protein binding
W	0005634	cellular_component	nucleus
W	0005829	cellular_component	cytosol
W	0008540	cellular_component	proteasome regulatory particle, base subcomplex
W	0022624	cellular_component	proteasome accessory complex
W	0031593	molecular_function	polyubiquitin modification-dependent protein binding
W	0042044	biological_process	fluid transport
W	0042802	molecular_function	identical protein binding
W	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
W	0060090	molecular_function	molecular adaptor activity
Y	0000502	cellular_component	proteasome complex
Y	0000724	biological_process	double-strand break repair via homologous recombination
Y	0005634	cellular_component	nucleus
Y	0006406	biological_process	mRNA export from nucleus
Y	0008541	cellular_component	proteasome regulatory particle, lid subcomplex
Y	0032039	cellular_component	integrator complex
Y	0032991	cellular_component	protein-containing complex
Y	0043248	biological_process	proteasome assembly
Z	0000502	cellular_component	proteasome complex
Z	0005634	cellular_component	nucleus
Z	0005838	cellular_component	proteasome regulatory particle
Z	0008540	cellular_component	proteasome regulatory particle, base subcomplex
Z	0022624	cellular_component	proteasome accessory complex
Z	0030234	molecular_function	enzyme regulator activity
Z	0034515	cellular_component	proteasome storage granule
Z	0042176	biological_process	regulation of protein catabolic process
Z	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process

Functional Information from PROSITE/UniProt

site_id	PS00388
Number of Residues	23
Details	PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdlsaStFSPdGRvfQVEYAmkA

Chain	Residue	Details
G	TYR8-ALA30
B	TYR6-ALA28
D	TYR3-ALA25
E	TYR8-GLY30
A	PHE9-ALA31
C	TYR5-ALA27
F	TYR6-ALA28

---

site_id	PS00012
Number of Residues	16
Details	PHOSPHOPANTETHEINE Phosphopantetheine attachment site. TTGVSSSGPAATSGGI

Chain	Residue	Details
T	THR75-ILE90

---

site_id	PS00854
Number of Residues	48
Details	PROTEASOME_BETA_1 Proteasome beta-type subunits signature. LAFkFqhGVIVAADsratagpyiasqtvk.KvieinpyllgtmaGgaAD

Chain	Residue	Details
5	LEU63-ASP110
7	LEU56-ASP103
2	ALA47-ASP94
C	LEU35-ASP82
3	MET12-ASP59
6	LEU40-ASP87
4	ILE5-ASP52
1	MET37-ASP84

---

site_id	PS00674
Number of Residues	19
Details	AAA AAA-protein family signature. IkVLmATNrpdtLDpALm.R

Chain	Residue	Details
H	ILE315-ARG333
I	VAL325-ARG343
K	VAL305-ARG323
J	ILE289-ARG307
L	VAL287-ARG305
M	VAL329-ARG347

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	BINDING: BINDING => ECO:0000255

Chain	Residue	Details
M	GLY230
C	SER75
C	SER173

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: N-acetylmethionine => ECO:0000250|UniProtKB:P17980

Chain	Residue	Details
M	MET4
H	LYS422
6	SER161

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P17980

Chain	Residue	Details
M	SER12
M	SER379
Z	SER363

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P62195

Chain	Residue	Details
J	LYS222
B	TYR76

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:P43686

Chain	Residue	Details
K	LYS397
K	LYS401
F	LYS208

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	LIPID: N-myristoyl glycine => ECO:0000250|UniProtKB:P62191

Chain	Residue	Details
I	GLY2

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000250|UniProtKB:P62191

Chain	Residue	Details
I	LYS237

---