6CIK

Pre-Reaction Complex, RAG1(E962Q)/2-intact/nicked 12/23RSS complex in Mn2+

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004519	molecular_function	endonuclease activity
A	0033151	biological_process	V(D)J recombination
A	0043565	molecular_function	sequence-specific DNA binding
A	0061630	molecular_function	ubiquitin protein ligase activity
B	0003677	molecular_function	DNA binding
B	0005634	cellular_component	nucleus
B	0006310	biological_process	DNA recombination
C	0004519	molecular_function	endonuclease activity
C	0033151	biological_process	V(D)J recombination
C	0043565	molecular_function	sequence-specific DNA binding
C	0061630	molecular_function	ubiquitin protein ligase activity
D	0003677	molecular_function	DNA binding
D	0005634	cellular_component	nucleus
D	0006310	biological_process	DNA recombination
N	0003677	molecular_function	DNA binding
N	0005634	cellular_component	nucleus

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 1101

Chain	Residue
A	CYS727
A	CYS730
A	HIS937
A	HIS942

---

site_id	AC2
Number of Residues	3
Details	binding site for residue MN A 1102

Chain	Residue
A	ASP600
A	GLU662
A	ASP708

---

site_id	AC3
Number of Residues	4
Details	binding site for residue ZN C 1101

Chain	Residue
C	HIS937
C	HIS942
C	CYS727
C	CYS730

---

site_id	AC4
Number of Residues	3
Details	binding site for residue MN C 1102

Chain	Residue
C	ASP600
C	GLU662
C	ASP708

---

Functional Information from PROSITE/UniProt

site_id	PS00353
Number of Residues	12
Details	HMG_BOX_1 HMG box A DNA-binding domain signature. FSKKCsERWKTM

Chain	Residue	Details
N	PHE41-MET52

---

site_id	PS00435
Number of Residues	11
Details	PEROXIDASE_1 Peroxidases proximal heme-ligand signature. DTVYILGGHSL

Chain	Residue	Details
B	ASP214-LEU224

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	Binding site: {"evidences":[{"evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Site: {"description":"Essential for DNA hairpin formation, participates in base-stacking interactions near the cleavage site"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	16
Details	Motif: {"description":"Nuclear localization signal (NLS) 1","evidences":[{"source":"UniProtKB","id":"P63159","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	Modified residue: {"description":"Cysteine sulfonic acid (-SO3H); alternate","evidences":[{"source":"UniProtKB","id":"P63159","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P10103","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21406692","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P63158","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	1
Details	Modified residue: {"description":"Cysteine sulfonic acid (-SO3H)","evidences":[{"source":"UniProtKB","id":"P63159","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	4
Details	Cross-link: {"description":"Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-?)","evidences":[{"source":"PubMed","id":"29618516","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---