6CIK
Pre-Reaction Complex, RAG1(E962Q)/2-intact/nicked 12/23RSS complex in Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-02-23 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 156.423, 123.149, 186.327 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 37.842 - 3.150 |
| R-factor | 0.2109 |
| Rwork | 0.209 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wwx |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.120 | 1.039 |
| Rmeas | 0.141 | 1.208 |
| Rpim | 0.072 | 0.614 |
| Number of reflections | 58996 | 5870 |
| <I/σ(I)> | 8.94 | 1.19 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.8 | 3.9 |
| CC(1/2) | 0.394 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 277 | 12-16% PEG 3350, 200 mM NaNO3, 50 mM HEPES, pH 6.9 |
