6C24

Cryo-EM structure of PRC2 bound to cofactors AEBP2 and JARID2 in the Extended Active State

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000122	biological_process	negative regulation of transcription by RNA polymerase II
A	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
A	0001222	molecular_function	transcription corepressor binding
A	0001739	cellular_component	sex chromatin
A	0003682	molecular_function	chromatin binding
A	0003723	molecular_function	RNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005677	cellular_component	chromatin silencing complex
A	0005730	cellular_component	nucleolus
A	0006325	biological_process	chromatin organization
A	0008047	molecular_function	enzyme activator activity
A	0008283	biological_process	cell population proliferation
A	0008284	biological_process	positive regulation of cell population proliferation
A	0016586	cellular_component	RSC-type complex
A	0016604	cellular_component	nuclear body
A	0031490	molecular_function	chromatin DNA binding
A	0032993	cellular_component	protein-DNA complex
A	0035064	molecular_function	methylated histone binding
A	0035098	cellular_component	ESC/E(Z) complex
A	0042054	molecular_function	histone methyltransferase activity
A	0043565	molecular_function	sequence-specific DNA binding
A	0045596	biological_process	negative regulation of cell differentiation
A	0046872	molecular_function	metal ion binding
A	0048709	biological_process	oligodendrocyte differentiation
A	0060816	biological_process	random inactivation of X chromosome
A	0106222	molecular_function	lncRNA binding
A	0140718	biological_process	facultative heterochromatin formation
A	1990841	molecular_function	promoter-specific chromatin binding
A	1990904	cellular_component	ribonucleoprotein complex
C	0000082	biological_process	G1/S transition of mitotic cell cycle
C	0000122	biological_process	negative regulation of transcription by RNA polymerase II
C	0000781	cellular_component	chromosome, telomeric region
C	0000785	cellular_component	chromatin
C	0000976	molecular_function	transcription cis-regulatory region binding
C	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
C	0000979	molecular_function	RNA polymerase II core promoter sequence-specific DNA binding
C	0001222	molecular_function	transcription corepressor binding
C	0001932	biological_process	regulation of protein phosphorylation
C	0003677	molecular_function	DNA binding
C	0003682	molecular_function	chromatin binding
C	0003714	molecular_function	transcription corepressor activity
C	0003723	molecular_function	RNA binding
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005677	cellular_component	chromatin silencing complex
C	0005694	cellular_component	chromosome
C	0005721	cellular_component	pericentric heterochromatin
C	0006306	biological_process	obsolete DNA methylation
C	0006325	biological_process	chromatin organization
C	0006338	biological_process	chromatin remodeling
C	0006355	biological_process	regulation of DNA-templated transcription
C	0006357	biological_process	regulation of transcription by RNA polymerase II
C	0008168	molecular_function	methyltransferase activity
C	0008284	biological_process	positive regulation of cell population proliferation
C	0009913	biological_process	epidermal cell differentiation
C	0010468	biological_process	regulation of gene expression
C	0010629	biological_process	negative regulation of gene expression
C	0010718	biological_process	positive regulation of epithelial to mesenchymal transition
C	0014013	biological_process	regulation of gliogenesis
C	0014834	biological_process	skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration
C	0014898	biological_process	cardiac muscle hypertrophy in response to stress
C	0016279	molecular_function	protein-lysine N-methyltransferase activity
C	0021695	biological_process	cerebellar cortex development
C	0021766	biological_process	hippocampus development
C	0030097	biological_process	hemopoiesis
C	0030183	biological_process	B cell differentiation
C	0030216	biological_process	keratinocyte differentiation
C	0030335	biological_process	positive regulation of cell migration
C	0031048	biological_process	regulatory ncRNA-mediated heterochromatin formation
C	0031490	molecular_function	chromatin DNA binding
C	0031507	biological_process	heterochromatin formation
C	0031509	biological_process	subtelomeric heterochromatin formation
C	0032259	biological_process	methylation
C	0032355	biological_process	response to estradiol
C	0034244	biological_process	negative regulation of transcription elongation by RNA polymerase II
C	0035098	cellular_component	ESC/E(Z) complex
C	0035984	biological_process	cellular response to trichostatin A
C	0036211	biological_process	protein modification process
C	0036333	biological_process	hepatocyte homeostasis
C	0042054	molecular_function	histone methyltransferase activity
C	0042127	biological_process	regulation of cell population proliferation
C	0042752	biological_process	regulation of circadian rhythm
C	0043021	molecular_function	ribonucleoprotein complex binding
C	0043406	biological_process	positive regulation of MAP kinase activity
C	0043547	biological_process	positive regulation of GTPase activity
C	0043565	molecular_function	sequence-specific DNA binding
C	0045120	cellular_component	pronucleus
C	0045202	cellular_component	synapse
C	0045605	biological_process	negative regulation of epidermal cell differentiation
C	0045617	biological_process	negative regulation of keratinocyte differentiation
C	0045814	biological_process	negative regulation of gene expression, epigenetic
C	0045892	biological_process	negative regulation of DNA-templated transcription
C	0046976	molecular_function	histone H3K27 methyltransferase activity
C	0048387	biological_process	negative regulation of retinoic acid receptor signaling pathway
C	0048511	biological_process	rhythmic process
C	0048863	biological_process	stem cell differentiation
C	0050767	biological_process	regulation of neurogenesis
C	0051154	biological_process	negative regulation of striated muscle cell differentiation
C	0051932	biological_process	synaptic transmission, GABAergic
C	0070301	biological_process	cellular response to hydrogen peroxide
C	0070314	biological_process	G1 to G0 transition
C	0070878	molecular_function	primary miRNA binding
C	0071168	biological_process	protein localization to chromatin
C	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
C	0090183	biological_process	regulation of kidney development
C	0097421	biological_process	liver regeneration
C	0106222	molecular_function	lncRNA binding
C	0140718	biological_process	facultative heterochromatin formation
C	0140938	molecular_function	histone H3 methyltransferase activity
C	0140951	molecular_function	histone H3K27 trimethyltransferase activity
C	1900006	biological_process	positive regulation of dendrite development
C	1900016	biological_process	negative regulation of cytokine production involved in inflammatory response
C	1902808	biological_process	positive regulation of cell cycle G1/S phase transition
C	1904772	biological_process	response to tetrachloromethane
C	1990841	molecular_function	promoter-specific chromatin binding
C	2000134	biological_process	negative regulation of G1/S transition of mitotic cell cycle
C	2000737	biological_process	negative regulation of stem cell differentiation
K	0000082	biological_process	G1/S transition of mitotic cell cycle
K	0000122	biological_process	negative regulation of transcription by RNA polymerase II
K	0000781	cellular_component	chromosome, telomeric region
K	0000785	cellular_component	chromatin
K	0000976	molecular_function	transcription cis-regulatory region binding
K	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
K	0000979	molecular_function	RNA polymerase II core promoter sequence-specific DNA binding
K	0001222	molecular_function	transcription corepressor binding
K	0001932	biological_process	regulation of protein phosphorylation
K	0003677	molecular_function	DNA binding
K	0003682	molecular_function	chromatin binding
K	0003714	molecular_function	transcription corepressor activity
K	0003723	molecular_function	RNA binding
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005677	cellular_component	chromatin silencing complex
K	0005694	cellular_component	chromosome
K	0005721	cellular_component	pericentric heterochromatin
K	0006306	biological_process	obsolete DNA methylation
K	0006325	biological_process	chromatin organization
K	0006338	biological_process	chromatin remodeling
K	0006355	biological_process	regulation of DNA-templated transcription
K	0006357	biological_process	regulation of transcription by RNA polymerase II
K	0008168	molecular_function	methyltransferase activity
K	0008284	biological_process	positive regulation of cell population proliferation
K	0009913	biological_process	epidermal cell differentiation
K	0010468	biological_process	regulation of gene expression
K	0010629	biological_process	negative regulation of gene expression
K	0010718	biological_process	positive regulation of epithelial to mesenchymal transition
K	0014013	biological_process	regulation of gliogenesis
K	0014834	biological_process	skeletal muscle satellite cell maintenance involved in skeletal muscle regeneration
K	0014898	biological_process	cardiac muscle hypertrophy in response to stress
K	0016279	molecular_function	protein-lysine N-methyltransferase activity
K	0021695	biological_process	cerebellar cortex development
K	0021766	biological_process	hippocampus development
K	0030097	biological_process	hemopoiesis
K	0030183	biological_process	B cell differentiation
K	0030216	biological_process	keratinocyte differentiation
K	0030335	biological_process	positive regulation of cell migration
K	0031048	biological_process	regulatory ncRNA-mediated heterochromatin formation
K	0031490	molecular_function	chromatin DNA binding
K	0031507	biological_process	heterochromatin formation
K	0031509	biological_process	subtelomeric heterochromatin formation
K	0032259	biological_process	methylation
K	0032355	biological_process	response to estradiol
K	0034244	biological_process	negative regulation of transcription elongation by RNA polymerase II
K	0035098	cellular_component	ESC/E(Z) complex
K	0035984	biological_process	cellular response to trichostatin A
K	0036211	biological_process	protein modification process
K	0036333	biological_process	hepatocyte homeostasis
K	0042054	molecular_function	histone methyltransferase activity
K	0042127	biological_process	regulation of cell population proliferation
K	0042752	biological_process	regulation of circadian rhythm
K	0043021	molecular_function	ribonucleoprotein complex binding
K	0043406	biological_process	positive regulation of MAP kinase activity
K	0043547	biological_process	positive regulation of GTPase activity
K	0043565	molecular_function	sequence-specific DNA binding
K	0045120	cellular_component	pronucleus
K	0045202	cellular_component	synapse
K	0045605	biological_process	negative regulation of epidermal cell differentiation
K	0045617	biological_process	negative regulation of keratinocyte differentiation
K	0045814	biological_process	negative regulation of gene expression, epigenetic
K	0045892	biological_process	negative regulation of DNA-templated transcription
K	0046976	molecular_function	histone H3K27 methyltransferase activity
K	0048387	biological_process	negative regulation of retinoic acid receptor signaling pathway
K	0048511	biological_process	rhythmic process
K	0048863	biological_process	stem cell differentiation
K	0050767	biological_process	regulation of neurogenesis
K	0051154	biological_process	negative regulation of striated muscle cell differentiation
K	0051932	biological_process	synaptic transmission, GABAergic
K	0070301	biological_process	cellular response to hydrogen peroxide
K	0070314	biological_process	G1 to G0 transition
K	0070878	molecular_function	primary miRNA binding
K	0071168	biological_process	protein localization to chromatin
K	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
K	0090183	biological_process	regulation of kidney development
K	0097421	biological_process	liver regeneration
K	0106222	molecular_function	lncRNA binding
K	0140718	biological_process	facultative heterochromatin formation
K	0140938	molecular_function	histone H3 methyltransferase activity
K	0140951	molecular_function	histone H3K27 trimethyltransferase activity
K	1900006	biological_process	positive regulation of dendrite development
K	1900016	biological_process	negative regulation of cytokine production involved in inflammatory response
K	1902808	biological_process	positive regulation of cell cycle G1/S phase transition
K	1904772	biological_process	response to tetrachloromethane
K	1990841	molecular_function	promoter-specific chromatin binding
K	2000134	biological_process	negative regulation of G1/S transition of mitotic cell cycle
K	2000737	biological_process	negative regulation of stem cell differentiation
L	0000122	biological_process	negative regulation of transcription by RNA polymerase II
L	0001222	molecular_function	transcription corepressor binding
L	0003682	molecular_function	chromatin binding
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005694	cellular_component	chromosome
L	0005829	cellular_component	cytosol
L	0006325	biological_process	chromatin organization
L	0008047	molecular_function	enzyme activator activity
L	0021510	biological_process	spinal cord development
L	0031491	molecular_function	nucleosome binding
L	0031507	biological_process	heterochromatin formation
L	0035098	cellular_component	ESC/E(Z) complex
L	0042054	molecular_function	histone methyltransferase activity
L	0042802	molecular_function	identical protein binding
L	0045892	biological_process	negative regulation of DNA-templated transcription
M	0000122	biological_process	negative regulation of transcription by RNA polymerase II
M	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
M	0001222	molecular_function	transcription corepressor binding
M	0001739	cellular_component	sex chromatin
M	0003682	molecular_function	chromatin binding
M	0003723	molecular_function	RNA binding
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005677	cellular_component	chromatin silencing complex
M	0005730	cellular_component	nucleolus
M	0006325	biological_process	chromatin organization
M	0008047	molecular_function	enzyme activator activity
M	0008283	biological_process	cell population proliferation
M	0008284	biological_process	positive regulation of cell population proliferation
M	0016586	cellular_component	RSC-type complex
M	0016604	cellular_component	nuclear body
M	0031490	molecular_function	chromatin DNA binding
M	0032993	cellular_component	protein-DNA complex
M	0035064	molecular_function	methylated histone binding
M	0035098	cellular_component	ESC/E(Z) complex
M	0042054	molecular_function	histone methyltransferase activity
M	0043565	molecular_function	sequence-specific DNA binding
M	0045596	biological_process	negative regulation of cell differentiation
M	0046872	molecular_function	metal ion binding
M	0048709	biological_process	oligodendrocyte differentiation
M	0060816	biological_process	random inactivation of X chromosome
M	0106222	molecular_function	lncRNA binding
M	0140718	biological_process	facultative heterochromatin formation
M	1990841	molecular_function	promoter-specific chromatin binding
M	1990904	cellular_component	ribonucleoprotein complex
N	0000118	cellular_component	histone deacetylase complex
N	0000122	biological_process	negative regulation of transcription by RNA polymerase II
N	0000781	cellular_component	chromosome, telomeric region
N	0000785	cellular_component	chromatin
N	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005694	cellular_component	chromosome
N	0005829	cellular_component	cytosol
N	0006260	biological_process	DNA replication
N	0006325	biological_process	chromatin organization
N	0006334	biological_process	nucleosome assembly
N	0006335	biological_process	DNA replication-dependent chromatin assembly
N	0006338	biological_process	chromatin remodeling
N	0006355	biological_process	regulation of DNA-templated transcription
N	0007420	biological_process	brain development
N	0008094	molecular_function	ATP-dependent activity, acting on DNA
N	0008285	biological_process	negative regulation of cell population proliferation
N	0016581	cellular_component	NuRD complex
N	0016589	cellular_component	NURF complex
N	0030336	biological_process	negative regulation of cell migration
N	0030512	biological_process	negative regulation of transforming growth factor beta receptor signaling pathway
N	0031492	molecular_function	nucleosomal DNA binding
N	0032991	cellular_component	protein-containing complex
N	0033186	cellular_component	CAF-1 complex
N	0035098	cellular_component	ESC/E(Z) complex
N	0042393	molecular_function	histone binding
N	0042659	biological_process	regulation of cell fate specification
N	0042826	molecular_function	histone deacetylase binding
N	0045892	biological_process	negative regulation of DNA-templated transcription
N	0045893	biological_process	positive regulation of DNA-templated transcription
N	0070822	cellular_component	Sin3-type complex
N	1902455	biological_process	negative regulation of stem cell population maintenance
N	1902459	biological_process	positive regulation of stem cell population maintenance
N	1904949	cellular_component	ATPase complex
N	2000736	biological_process	regulation of stem cell differentiation
Q	0000122	biological_process	negative regulation of transcription by RNA polymerase II
Q	0000978	molecular_function	RNA polymerase II cis-regulatory region sequence-specific DNA binding
Q	0001222	molecular_function	transcription corepressor binding
Q	0001739	cellular_component	sex chromatin
Q	0003682	molecular_function	chromatin binding
Q	0003723	molecular_function	RNA binding
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005654	cellular_component	nucleoplasm
Q	0005677	cellular_component	chromatin silencing complex
Q	0005730	cellular_component	nucleolus
Q	0006325	biological_process	chromatin organization
Q	0008047	molecular_function	enzyme activator activity
Q	0008283	biological_process	cell population proliferation
Q	0008284	biological_process	positive regulation of cell population proliferation
Q	0016586	cellular_component	RSC-type complex
Q	0016604	cellular_component	nuclear body
Q	0031490	molecular_function	chromatin DNA binding
Q	0032993	cellular_component	protein-DNA complex
Q	0035064	molecular_function	methylated histone binding
Q	0035098	cellular_component	ESC/E(Z) complex
Q	0042054	molecular_function	histone methyltransferase activity
Q	0043565	molecular_function	sequence-specific DNA binding
Q	0045596	biological_process	negative regulation of cell differentiation
Q	0046872	molecular_function	metal ion binding
Q	0048709	biological_process	oligodendrocyte differentiation
Q	0060816	biological_process	random inactivation of X chromosome
Q	0106222	molecular_function	lncRNA binding
Q	0140718	biological_process	facultative heterochromatin formation
Q	1990841	molecular_function	promoter-specific chromatin binding
Q	1990904	cellular_component	ribonucleoprotein complex

Functional Information from PROSITE/UniProt

site_id	PS00028
Number of Residues	22
Details	ZINC_FINGER_C2H2_1 Zinc finger C2H2 type domain signature. Cpw..CtlnCrklysllkHlklc.H

Chain	Residue	Details
A	CYS450-HIS471
P	CYS55-HIS78
P	CYS122-HIS144

---

site_id	PS00678
Number of Residues	15
Details	WD_REPEATS_1 Trp-Asp (WD) repeats signature. LLSVskDhALRLWNI

Chain	Residue	Details
L	LEU206-ILE220
N	LEU193-ILE207
N	LEU289-LEU303
N	LEU333-LEU347

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	25
Details	ZN_FING: C2H2-type 1 => ECO:0000255|PROSITE-ProRule:PRU00042

Chain	Residue	Details
P	TYR53-HIS78
C	SER21
Q	LEU448-HIS471

---

site_id	SWS_FT_FI2
Number of Residues	22
Details	ZN_FING: C2H2-type 2; degenerate => ECO:0000255|PROSITE-ProRule:PRU00042

Chain	Residue	Details
P	LYS92-HIS114
K	SER363
C	SER76
C	SER363

---

site_id	SWS_FT_FI3
Number of Residues	24
Details	ZN_FING: C2H2-type 3 => ECO:0000255|PROSITE-ProRule:PRU00042

Chain	Residue	Details
P	PHE120-HIS144
C	THR339
A	SER726
M	SER20
M	SER541
M	SER726
Q	SER20
Q	SER541
Q	SER726

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648

Chain	Residue	Details
P	SER2
P	SER3
L	LYS268
L	LYS284

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692

Chain	Residue	Details
P	SER182
C	SER366
Q	SER583

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternate

Chain	Residue	Details
N	LYS4
C	THR367
M	LYS72
M	LYS73
Q	LYS72
Q	LYS73

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744|PubMed:25755297, ECO:0007744|PubMed:28112733

Chain	Residue	Details
K	THR487
N	LYS160
A	LYS390
M	LYS223
M	LYS390
Q	LYS223
Q	LYS390

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	CARBOHYD: O-linked (GlcNAc) serine => ECO:0000269|PubMed:24474760

Chain	Residue	Details
K	SER75
C	SER75
Q	LYS75

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
K	LYS634
C	LYS634

---