6BYR

Structures of the PKA RI alpha holoenzyme with the FLHCC driver J-PKAc alpha or native PKAc alpha

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000287	molecular_function	magnesium ion binding
A	0001669	cellular_component	acrosomal vesicle
A	0001707	biological_process	mesoderm formation
A	0001843	biological_process	neural tube closure
A	0002027	biological_process	regulation of heart rate
A	0003091	biological_process	renal water homeostasis
A	0004672	molecular_function	protein kinase activity
A	0004674	molecular_function	protein serine/threonine kinase activity
A	0004691	molecular_function	cAMP-dependent protein kinase activity
A	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005759	cellular_component	mitochondrial matrix
A	0005813	cellular_component	centrosome
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005930	cellular_component	axoneme
A	0005952	cellular_component	cAMP-dependent protein kinase complex
A	0006397	biological_process	mRNA processing
A	0006468	biological_process	protein phosphorylation
A	0006611	biological_process	protein export from nucleus
A	0007165	biological_process	signal transduction
A	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
A	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
A	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
A	0016241	biological_process	regulation of macroautophagy
A	0016607	cellular_component	nuclear speck
A	0018105	biological_process	peptidyl-serine phosphorylation
A	0019221	biological_process	cytokine-mediated signaling pathway
A	0019901	molecular_function	protein kinase binding
A	0019904	molecular_function	protein domain specific binding
A	0030145	molecular_function	manganese ion binding
A	0031410	cellular_component	cytoplasmic vesicle
A	0031514	cellular_component	motile cilium
A	0031594	cellular_component	neuromuscular junction
A	0031625	molecular_function	ubiquitin protein ligase binding
A	0032024	biological_process	positive regulation of insulin secretion
A	0032703	biological_process	negative regulation of interleukin-2 production
A	0034237	molecular_function	protein kinase A regulatory subunit binding
A	0034380	biological_process	high-density lipoprotein particle assembly
A	0034605	biological_process	cellular response to heat
A	0034704	cellular_component	calcium channel complex
A	0035694	biological_process	mitochondrial protein catabolic process
A	0036126	cellular_component	sperm flagellum
A	0044853	cellular_component	plasma membrane raft
A	0045542	biological_process	positive regulation of cholesterol biosynthetic process
A	0045667	biological_process	regulation of osteoblast differentiation
A	0045722	biological_process	positive regulation of gluconeogenesis
A	0045879	biological_process	negative regulation of smoothened signaling pathway
A	0046827	biological_process	positive regulation of protein export from nucleus
A	0048240	biological_process	sperm capacitation
A	0048471	cellular_component	perinuclear region of cytoplasm
A	0050804	biological_process	modulation of chemical synaptic transmission
A	0050850	biological_process	positive regulation of calcium-mediated signaling
A	0051726	biological_process	regulation of cell cycle
A	0055117	biological_process	regulation of cardiac muscle contraction
A	0061136	biological_process	regulation of proteasomal protein catabolic process
A	0070062	cellular_component	extracellular exosome
A	0070417	biological_process	cellular response to cold
A	0070613	biological_process	regulation of protein processing
A	0071333	biological_process	cellular response to glucose stimulus
A	0071374	biological_process	cellular response to parathyroid hormone stimulus
A	0071377	biological_process	cellular response to glucagon stimulus
A	0071872	biological_process	cellular response to epinephrine stimulus
A	0086064	biological_process	cell communication by electrical coupling involved in cardiac conduction
A	0097546	cellular_component	ciliary base
A	0098794	cellular_component	postsynapse
A	0098978	cellular_component	glutamatergic synapse
A	0099103	molecular_function	channel activator activity
A	0099170	biological_process	postsynaptic modulation of chemical synaptic transmission
A	0106310	molecular_function	protein serine kinase activity
A	1903779	biological_process	regulation of cardiac conduction
A	1904262	biological_process	negative regulation of TORC1 signaling
A	1904539	biological_process	negative regulation of glycolytic process through fructose-6-phosphate
A	1990044	biological_process	protein localization to lipid droplet
A	2000810	biological_process	regulation of bicellular tight junction assembly
B	0001707	biological_process	mesoderm formation
B	0001772	cellular_component	immunological synapse
B	0001932	biological_process	regulation of protein phosphorylation
B	0004862	molecular_function	cAMP-dependent protein kinase inhibitor activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005771	cellular_component	multivesicular body
B	0005813	cellular_component	centrosome
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0005930	cellular_component	axoneme
B	0005952	cellular_component	cAMP-dependent protein kinase complex
B	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
B	0007507	biological_process	heart development
B	0008603	molecular_function	cAMP-dependent protein kinase regulator activity
B	0010629	biological_process	negative regulation of gene expression
B	0019904	molecular_function	protein domain specific binding
B	0030552	molecular_function	cAMP binding
B	0031588	cellular_component	nucleotide-activated protein kinase complex
B	0031594	cellular_component	neuromuscular junction
B	0031625	molecular_function	ubiquitin protein ligase binding
B	0032024	biological_process	positive regulation of insulin secretion
B	0032991	cellular_component	protein-containing complex
B	0034236	molecular_function	protein kinase A catalytic subunit binding
B	0042802	molecular_function	identical protein binding
B	0044853	cellular_component	plasma membrane raft
B	0045202	cellular_component	synapse
B	0045214	biological_process	sarcomere organization
B	0046007	biological_process	negative regulation of activated T cell proliferation
B	0060038	biological_process	cardiac muscle cell proliferation
B	0071377	biological_process	cellular response to glucagon stimulus
B	0098978	cellular_component	glutamatergic synapse
B	0120212	cellular_component	sperm head-tail coupling apparatus
B	0141162	biological_process	negative regulation of cAMP/PKA signal transduction
C	0000287	molecular_function	magnesium ion binding
C	0001669	cellular_component	acrosomal vesicle
C	0001707	biological_process	mesoderm formation
C	0001843	biological_process	neural tube closure
C	0002027	biological_process	regulation of heart rate
C	0003091	biological_process	renal water homeostasis
C	0004672	molecular_function	protein kinase activity
C	0004674	molecular_function	protein serine/threonine kinase activity
C	0004691	molecular_function	cAMP-dependent protein kinase activity
C	0004712	molecular_function	protein serine/threonine/tyrosine kinase activity
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005739	cellular_component	mitochondrion
C	0005759	cellular_component	mitochondrial matrix
C	0005813	cellular_component	centrosome
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005930	cellular_component	axoneme
C	0005952	cellular_component	cAMP-dependent protein kinase complex
C	0006397	biological_process	mRNA processing
C	0006468	biological_process	protein phosphorylation
C	0006611	biological_process	protein export from nucleus
C	0007165	biological_process	signal transduction
C	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
C	0007193	biological_process	adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway
C	0010881	biological_process	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
C	0016241	biological_process	regulation of macroautophagy
C	0016607	cellular_component	nuclear speck
C	0018105	biological_process	peptidyl-serine phosphorylation
C	0019221	biological_process	cytokine-mediated signaling pathway
C	0019901	molecular_function	protein kinase binding
C	0019904	molecular_function	protein domain specific binding
C	0030145	molecular_function	manganese ion binding
C	0031410	cellular_component	cytoplasmic vesicle
C	0031514	cellular_component	motile cilium
C	0031594	cellular_component	neuromuscular junction
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0032024	biological_process	positive regulation of insulin secretion
C	0032703	biological_process	negative regulation of interleukin-2 production
C	0034237	molecular_function	protein kinase A regulatory subunit binding
C	0034380	biological_process	high-density lipoprotein particle assembly
C	0034605	biological_process	cellular response to heat
C	0034704	cellular_component	calcium channel complex
C	0035694	biological_process	mitochondrial protein catabolic process
C	0036126	cellular_component	sperm flagellum
C	0044853	cellular_component	plasma membrane raft
C	0045542	biological_process	positive regulation of cholesterol biosynthetic process
C	0045667	biological_process	regulation of osteoblast differentiation
C	0045722	biological_process	positive regulation of gluconeogenesis
C	0045879	biological_process	negative regulation of smoothened signaling pathway
C	0046827	biological_process	positive regulation of protein export from nucleus
C	0048240	biological_process	sperm capacitation
C	0048471	cellular_component	perinuclear region of cytoplasm
C	0050804	biological_process	modulation of chemical synaptic transmission
C	0050850	biological_process	positive regulation of calcium-mediated signaling
C	0051726	biological_process	regulation of cell cycle
C	0055117	biological_process	regulation of cardiac muscle contraction
C	0061136	biological_process	regulation of proteasomal protein catabolic process
C	0070062	cellular_component	extracellular exosome
C	0070417	biological_process	cellular response to cold
C	0070613	biological_process	regulation of protein processing
C	0071333	biological_process	cellular response to glucose stimulus
C	0071374	biological_process	cellular response to parathyroid hormone stimulus
C	0071377	biological_process	cellular response to glucagon stimulus
C	0071872	biological_process	cellular response to epinephrine stimulus
C	0086064	biological_process	cell communication by electrical coupling involved in cardiac conduction
C	0097546	cellular_component	ciliary base
C	0098794	cellular_component	postsynapse
C	0098978	cellular_component	glutamatergic synapse
C	0099103	molecular_function	channel activator activity
C	0099170	biological_process	postsynaptic modulation of chemical synaptic transmission
C	0106310	molecular_function	protein serine kinase activity
C	1903779	biological_process	regulation of cardiac conduction
C	1904262	biological_process	negative regulation of TORC1 signaling
C	1904539	biological_process	negative regulation of glycolytic process through fructose-6-phosphate
C	1990044	biological_process	protein localization to lipid droplet
C	2000810	biological_process	regulation of bicellular tight junction assembly
D	0001707	biological_process	mesoderm formation
D	0001772	cellular_component	immunological synapse
D	0001932	biological_process	regulation of protein phosphorylation
D	0004862	molecular_function	cAMP-dependent protein kinase inhibitor activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005771	cellular_component	multivesicular body
D	0005813	cellular_component	centrosome
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0005930	cellular_component	axoneme
D	0005952	cellular_component	cAMP-dependent protein kinase complex
D	0007189	biological_process	adenylate cyclase-activating G protein-coupled receptor signaling pathway
D	0007507	biological_process	heart development
D	0008603	molecular_function	cAMP-dependent protein kinase regulator activity
D	0010629	biological_process	negative regulation of gene expression
D	0019904	molecular_function	protein domain specific binding
D	0030552	molecular_function	cAMP binding
D	0031588	cellular_component	nucleotide-activated protein kinase complex
D	0031594	cellular_component	neuromuscular junction
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0032024	biological_process	positive regulation of insulin secretion
D	0032991	cellular_component	protein-containing complex
D	0034236	molecular_function	protein kinase A catalytic subunit binding
D	0042802	molecular_function	identical protein binding
D	0044853	cellular_component	plasma membrane raft
D	0045202	cellular_component	synapse
D	0045214	biological_process	sarcomere organization
D	0046007	biological_process	negative regulation of activated T cell proliferation
D	0060038	biological_process	cardiac muscle cell proliferation
D	0071377	biological_process	cellular response to glucagon stimulus
D	0098978	cellular_component	glutamatergic synapse
D	0120212	cellular_component	sperm head-tail coupling apparatus
D	0141162	biological_process	negative regulation of cAMP/PKA signal transduction

Functional Information from PDB Data

site_id	AC1
Number of Residues	22
Details	binding site for residue ATP A 501

Chain	Residue
A	GLY105
A	VAL178
A	GLU182
A	LYS223
A	GLU225
A	ASN226
A	LEU228
A	THR238
A	ASP239
A	PHE382
A	MG502
A	GLY107
A	MG503
B	ARG94
B	ALA97
A	SER108
A	PHE109
A	VAL112
A	ALA125
A	LYS127
A	MET175
A	GLU176

---

site_id	AC2
Number of Residues	3
Details	binding site for residue MG A 502

Chain	Residue
A	ASP221
A	ASP239
A	ATP501

---

site_id	AC3
Number of Residues	3
Details	binding site for residue MG A 503

Chain	Residue
A	ASN226
A	ASP239
A	ATP501

---

site_id	AC4
Number of Residues	21
Details	binding site for residue ATP C 501

Chain	Residue
C	GLY105
C	GLY107
C	SER108
C	PHE109
C	GLY110
C	VAL112
C	ALA125
C	LYS127
C	MET175
C	VAL178
C	GLU182
C	LYS223
C	GLU225
C	ASN226
C	LEU228
C	ASP239
C	PHE382
C	MG502
C	MG503
D	ARG94
D	ALA97

---

site_id	AC5
Number of Residues	3
Details	binding site for residue MG C 502

Chain	Residue
C	ASP221
C	ASP239
C	ATP501

---

site_id	AC6
Number of Residues	3
Details	binding site for residue MG C 503

Chain	Residue
C	ASN226
C	ASP239
C	ATP501

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. LGTGSFGRVMlVkhketgnh..........YAMK

Chain	Residue	Details
A	LEU104-LYS127

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. LiYrDLKpeNLLI

Chain	Residue	Details
A	LEU217-ILE229

---

site_id	PS00636
Number of Residues	20
Details	DNAJ_1 Nt-dnaJ domain signature. FKeIAeaYDvLsDprkREIF

Chain	Residue	Details
A	PHE44-PHE63

---

site_id	PS00888
Number of Residues	17
Details	CNMP_BINDING_1 Cyclic nucleotide-binding domain signature 1. VIqQGDeGDnFYVIdqG

Chain	Residue	Details
B	VAL162-GLY178
B	ILE280-GLY296

---

site_id	PS00889
Number of Residues	18
Details	CNMP_BINDING_2 Cyclic nucleotide-binding domain signature 2. FGElALiygtp......RAAtVkA

Chain	Residue	Details
B	PHE198-ALA215
B	PHE322-ALA339

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	BINDING:

Chain	Residue	Details
B	LEU135
D	ILE253
D	GLU324
D	ARG333
B	GLU200
B	ARG209
B	ILE253
B	GLU324
B	ARG333
D	LEU135
D	GLU200
D	ARG209

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: N-acetylalanine; in cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed => ECO:0000269|PubMed:6487597

Chain	Residue	Details
B	ALA1
D	ALA1

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P09456

Chain	Residue	Details
B	SER2
B	SER256
D	SER2
D	SER256
C	GLU176
C	LYS223

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P10644

Chain	Residue	Details
B	SER75
B	SER81
D	SER75
D	SER81

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q9DBC7

Chain	Residue	Details
B	SER99
D	SER99

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:12372837

Chain	Residue	Details
A	THR250
C	THR250

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphothreonine; by PDPK1 => ECO:0000269|PubMed:12372837, ECO:0000269|PubMed:16765046, ECO:0000269|PubMed:20137943, ECO:0000269|PubMed:20481595, ECO:0000269|PubMed:20732331, ECO:0000269|PubMed:21774789, ECO:0000269|Ref.43

Chain	Residue	Details
A	TPO252
C	TPO252

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	MOD_RES: Phosphotyrosine => ECO:0000250|UniProtKB:P05132

Chain	Residue	Details
A	TYR385
C	TYR385

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:16765046, ECO:0000269|PubMed:20137943, ECO:0000269|PubMed:20481595, ECO:0000269|PubMed:20732331, ECO:0000269|PubMed:21774789, ECO:0000269|Ref.43, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:24275569

Chain	Residue	Details
A	SEP393
C	SEP393

---