Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6BQT

Complex of 14-3-3 theta with an IRSp53 peptide doubly-phosphorylated at T340 and T360

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005925	cellular_component	focal adhesion
A	0006605	biological_process	protein targeting
A	0007165	biological_process	signal transduction
A	0007264	biological_process	small GTPase-mediated signal transduction
A	0008104	biological_process	protein localization
A	0016020	cellular_component	membrane
A	0019904	molecular_function	protein domain specific binding
A	0021762	biological_process	substantia nigra development
A	0032991	cellular_component	protein-containing complex
A	0034766	biological_process	negative regulation of monoatomic ion transmembrane transport
A	0042802	molecular_function	identical protein binding
A	0044325	molecular_function	transmembrane transporter binding
A	0045202	cellular_component	synapse
A	0045892	biological_process	negative regulation of DNA-templated transcription
A	0070062	cellular_component	extracellular exosome
A	0071889	molecular_function	14-3-3 protein binding
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005925	cellular_component	focal adhesion
B	0006605	biological_process	protein targeting
B	0007165	biological_process	signal transduction
B	0007264	biological_process	small GTPase-mediated signal transduction
B	0008104	biological_process	protein localization
B	0016020	cellular_component	membrane
B	0019904	molecular_function	protein domain specific binding
B	0021762	biological_process	substantia nigra development
B	0032991	cellular_component	protein-containing complex
B	0034766	biological_process	negative regulation of monoatomic ion transmembrane transport
B	0042802	molecular_function	identical protein binding
B	0044325	molecular_function	transmembrane transporter binding
B	0045202	cellular_component	synapse
B	0045892	biological_process	negative regulation of DNA-templated transcription
B	0070062	cellular_component	extracellular exosome
B	0071889	molecular_function	14-3-3 protein binding
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005925	cellular_component	focal adhesion
D	0006605	biological_process	protein targeting
D	0007165	biological_process	signal transduction
D	0007264	biological_process	small GTPase-mediated signal transduction
D	0008104	biological_process	protein localization
D	0016020	cellular_component	membrane
D	0019904	molecular_function	protein domain specific binding
D	0021762	biological_process	substantia nigra development
D	0032991	cellular_component	protein-containing complex
D	0034766	biological_process	negative regulation of monoatomic ion transmembrane transport
D	0042802	molecular_function	identical protein binding
D	0044325	molecular_function	transmembrane transporter binding
D	0045202	cellular_component	synapse
D	0045892	biological_process	negative regulation of DNA-templated transcription
D	0070062	cellular_component	extracellular exosome
D	0071889	molecular_function	14-3-3 protein binding
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005925	cellular_component	focal adhesion
E	0006605	biological_process	protein targeting
E	0007165	biological_process	signal transduction
E	0007264	biological_process	small GTPase-mediated signal transduction
E	0008104	biological_process	protein localization
E	0016020	cellular_component	membrane
E	0019904	molecular_function	protein domain specific binding
E	0021762	biological_process	substantia nigra development
E	0032991	cellular_component	protein-containing complex
E	0034766	biological_process	negative regulation of monoatomic ion transmembrane transport
E	0042802	molecular_function	identical protein binding
E	0044325	molecular_function	transmembrane transporter binding
E	0045202	cellular_component	synapse
E	0045892	biological_process	negative regulation of DNA-templated transcription
E	0070062	cellular_component	extracellular exosome
E	0071889	molecular_function	14-3-3 protein binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005925	cellular_component	focal adhesion
G	0006605	biological_process	protein targeting
G	0007165	biological_process	signal transduction
G	0007264	biological_process	small GTPase-mediated signal transduction
G	0008104	biological_process	protein localization
G	0016020	cellular_component	membrane
G	0019904	molecular_function	protein domain specific binding
G	0021762	biological_process	substantia nigra development
G	0032991	cellular_component	protein-containing complex
G	0034766	biological_process	negative regulation of monoatomic ion transmembrane transport
G	0042802	molecular_function	identical protein binding
G	0044325	molecular_function	transmembrane transporter binding
G	0045202	cellular_component	synapse
G	0045892	biological_process	negative regulation of DNA-templated transcription
G	0070062	cellular_component	extracellular exosome
G	0071889	molecular_function	14-3-3 protein binding
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005925	cellular_component	focal adhesion
H	0006605	biological_process	protein targeting
H	0007165	biological_process	signal transduction
H	0007264	biological_process	small GTPase-mediated signal transduction
H	0008104	biological_process	protein localization
H	0016020	cellular_component	membrane
H	0019904	molecular_function	protein domain specific binding
H	0021762	biological_process	substantia nigra development
H	0032991	cellular_component	protein-containing complex
H	0034766	biological_process	negative regulation of monoatomic ion transmembrane transport
H	0042802	molecular_function	identical protein binding
H	0044325	molecular_function	transmembrane transporter binding
H	0045202	cellular_component	synapse
H	0045892	biological_process	negative regulation of DNA-templated transcription
H	0070062	cellular_component	extracellular exosome
H	0071889	molecular_function	14-3-3 protein binding
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005925	cellular_component	focal adhesion
J	0006605	biological_process	protein targeting
J	0007165	biological_process	signal transduction
J	0007264	biological_process	small GTPase-mediated signal transduction
J	0008104	biological_process	protein localization
J	0016020	cellular_component	membrane
J	0019904	molecular_function	protein domain specific binding
J	0021762	biological_process	substantia nigra development
J	0032991	cellular_component	protein-containing complex
J	0034766	biological_process	negative regulation of monoatomic ion transmembrane transport
J	0042802	molecular_function	identical protein binding
J	0044325	molecular_function	transmembrane transporter binding
J	0045202	cellular_component	synapse
J	0045892	biological_process	negative regulation of DNA-templated transcription
J	0070062	cellular_component	extracellular exosome
J	0071889	molecular_function	14-3-3 protein binding
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005737	cellular_component	cytoplasm
K	0005829	cellular_component	cytosol
K	0005925	cellular_component	focal adhesion
K	0006605	biological_process	protein targeting
K	0007165	biological_process	signal transduction
K	0007264	biological_process	small GTPase-mediated signal transduction
K	0008104	biological_process	protein localization
K	0016020	cellular_component	membrane
K	0019904	molecular_function	protein domain specific binding
K	0021762	biological_process	substantia nigra development
K	0032991	cellular_component	protein-containing complex
K	0034766	biological_process	negative regulation of monoatomic ion transmembrane transport
K	0042802	molecular_function	identical protein binding
K	0044325	molecular_function	transmembrane transporter binding
K	0045202	cellular_component	synapse
K	0045892	biological_process	negative regulation of DNA-templated transcription
K	0070062	cellular_component	extracellular exosome
K	0071889	molecular_function	14-3-3 protein binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue PE4 A 301

Chain	Residue
A	LEU98
A	ASP102
A	ILE106
A	LYS122
A	ASN144
A	ALA148
A	GLU151

site_id	AC2
Number of Residues	8
Details	binding site for residue 1PE A 302

Chain	Residue
A	LYS9
A	ASP21
A	THR24
A	CYS25
B	ASP81
B	TYR82
A	MET1
A	GLU5

site_id	AC3
Number of Residues	7
Details	binding site for residue 1PE A 303

Chain	Residue
A	ASP20
A	ALA23
A	THR24
A	LYS27
A	TYR48
A	ARG55
A	GLU89

site_id	AC4
Number of Residues	3
Details	binding site for residue EDO B 301

Chain	Residue
A	GLN8
A	GLN15
B	LYS75

site_id	AC5
Number of Residues	6
Details	binding site for residue 1PE B 302

Chain	Residue
A	LEU78
A	ASP81
A	TYR82
B	LYS9
B	THR24
B	CYS25

site_id	AC6
Number of Residues	6
Details	binding site for residue PG4 B 303

Chain	Residue
B	ASP102
B	ILE106
B	LEU119
B	LYS122
B	ASN144
B	GLU151

site_id	AC7
Number of Residues	3
Details	binding site for residue GOL B 304

Chain	Residue
B	ARG55
B	SER92
B	ILE93

site_id	AC8
Number of Residues	4
Details	binding site for residue PG4 D 300

Chain	Residue
D	MET1
D	LYS9
D	CYS25
E	LYS85

site_id	AC9
Number of Residues	5
Details	binding site for residue EDO D 301

Chain	Residue
D	LYS122
D	ASN144
D	GLY147
D	ALA148
D	GLU151

site_id	AD1
Number of Residues	5
Details	binding site for residue PG4 E 300

Chain	Residue
D	LEU78
D	ASP81
E	MET1
E	LEU6
E	LYS9

site_id	AD2
Number of Residues	5
Details	binding site for residue PGE E 301

Chain	Residue
E	LEU98
E	PHE126
E	LEU129
E	ASP137
E	THR141

site_id	AD3
Number of Residues	2
Details	binding site for residue PEG E 302

Chain	Residue
E	GLU198
E	ALA201

site_id	AD4
Number of Residues	6
Details	binding site for residue 1PE G 300

Chain	Residue
G	ASP102
G	LEU119
G	LYS122
G	ASN144
G	ALA148
G	GLU151

site_id	AD5
Number of Residues	7
Details	binding site for residue 1PE H 300

Chain	Residue
G	LEU78
G	ASP81
G	TYR82
H	MET1
H	LYS9
H	ASP21
H	CYS25

site_id	AD6
Number of Residues	2
Details	binding site for residue GOL I 401

Chain	Residue
I	LEU341
I	PRO342

site_id	AD7
Number of Residues	5
Details	binding site for residue 1PE J 300

Chain	Residue
J	THR24
J	LYS27
J	TYR48
J	ARG55
J	GLU89

site_id	AD8
Number of Residues	5
Details	binding site for residue 1PE J 301

Chain	Residue
J	ARG91
J	LEU98
J	LYS122
J	ASP137
J	THR141

Functional Information from PROSITE/UniProt

site_id	PS00796
Number of Residues	11
Details	1433_1 14-3-3 proteins signature 1. RNLLSVAYKNV

Chain	Residue	Details
A	ARG41-VAL51

site_id	PS00797
Number of Residues	20
Details	1433_2 14-3-3 proteins signature 2. YKDSTLIMQLLRDNLTLWTS

Chain	Residue	Details
A	TYR211-SER230

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
C	SER336
G	ARG127
H	ARG56
H	ARG127
J	ARG56
J	ARG127
K	ARG56
K	ARG127
F	SER336
I	SER336
L	SER336
D	ARG56
D	ARG127
E	ARG56
E	ARG127
G	ARG56

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:20068231, ECO:0007744\|PubMed:23186163

Chain	Residue	Details
C	TPO340
F	TPO340
I	TPO340
L	TPO340
G	MET1
H	MET1
J	MET1
K	MET1

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18669648

Chain	Residue	Details
C	SER346
E	LYS3
E	LYS68
E	LYS115
G	LYS3
G	LYS68
G	LYS115
H	LYS3
H	LYS68
H	LYS115
J	LYS3
F	SER346
J	LYS68
J	LYS115
K	LYS3
K	LYS68
K	LYS115
I	SER346
L	SER346
B	LYS68
B	LYS115
D	LYS3
D	LYS68
D	LYS115

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: Phosphothreonine => ECO:0007744\|PubMed:23186163

Chain	Residue	Details
C	TPO360
F	TPO360
I	TPO360
L	TPO360
G	LYS49
H	LYS49
J	LYS49
K	LYS49

site_id	SWS_FT_FI5
Number of Residues	4
Details	MOD_RES: Phosphoserine => ECO:0007744\|PubMed:18669648, ECO:0007744\|PubMed:23186163, ECO:0007744\|PubMed:24275569

Chain	Residue	Details
C	SER366
G	TYR104
H	TYR82
H	TYR104
J	TYR82
J	TYR104
K	TYR82
K	TYR104
F	SER366
I	SER366
L	SER366
D	TYR82
D	TYR104
E	TYR82
E	TYR104
G	TYR82

site_id	SWS_FT_FI6
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P68254

Chain	Residue	Details
A	SER92
B	SER92
D	SER92
E	SER92
G	SER92
H	SER92
J	SER92
K	SER92

site_id	SWS_FT_FI7
Number of Residues	8
Details	MOD_RES: Phosphoserine => ECO:0000269\|PubMed:9360956, ECO:0007744\|PubMed:18669648

Chain	Residue	Details
A	SER232
B	SER232
D	SER232
E	SER232
G	SER232
H	SER232
J	SER232
K	SER232

site_id	SWS_FT_FI8
Number of Residues	16
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate => ECO:0007744\|PubMed:28112733

Chain	Residue	Details
A	LYS49
H	LYS49
J	LYS49
K	LYS49
B	LYS49
D	LYS49
E	LYS49
G	LYS49

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)