5XTE

Cryo-EM structure of human respiratory complex III (cytochrome bc1 complex)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005739	cellular_component	mitochondrion
A	0005743	cellular_component	mitochondrial inner membrane
A	0005750	cellular_component	mitochondrial respiratory chain complex III
A	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
A	0016020	cellular_component	membrane
A	0021539	biological_process	subthalamus development
A	0021548	biological_process	pons development
A	0021680	biological_process	cerebellar Purkinje cell layer development
A	0021766	biological_process	hippocampus development
A	0021794	biological_process	thalamus development
A	0021854	biological_process	hypothalamus development
A	0021860	biological_process	pyramidal neuron development
A	0030901	biological_process	midbrain development
A	0045333	biological_process	cellular respiration
A	0070469	cellular_component	respirasome
B	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
C	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
C	0016020	cellular_component	membrane
C	0051537	molecular_function	2 iron, 2 sulfur cluster binding
D	0005515	molecular_function	protein binding
D	0005739	cellular_component	mitochondrion
D	0005743	cellular_component	mitochondrial inner membrane
D	0005750	cellular_component	mitochondrial respiratory chain complex III
D	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
D	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
D	0016020	cellular_component	membrane
D	0045333	biological_process	cellular respiration
D	0070469	cellular_component	respirasome
D	1902600	biological_process	proton transmembrane transport
E	0005750	cellular_component	mitochondrial respiratory chain complex III
E	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
F	0005515	molecular_function	protein binding
F	0005739	cellular_component	mitochondrion
F	0005743	cellular_component	mitochondrial inner membrane
F	0005746	cellular_component	mitochondrial respirasome
F	0005750	cellular_component	mitochondrial respiratory chain complex III
F	0006119	biological_process	oxidative phosphorylation
F	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
F	0009060	biological_process	aerobic respiration
F	0016020	cellular_component	membrane
F	0045333	biological_process	cellular respiration
F	0070469	cellular_component	respirasome
G	0005739	cellular_component	mitochondrion
G	0005743	cellular_component	mitochondrial inner membrane
G	0005750	cellular_component	mitochondrial respiratory chain complex III
G	0006091	biological_process	generation of precursor metabolites and energy
G	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
G	0009055	molecular_function	electron transfer activity
G	0016020	cellular_component	membrane
G	0045333	biological_process	cellular respiration
G	0070469	cellular_component	respirasome
H	0009055	molecular_function	electron transfer activity
H	0020037	molecular_function	heme binding
J	0001666	biological_process	response to hypoxia
J	0005739	cellular_component	mitochondrion
J	0005743	cellular_component	mitochondrial inner membrane
J	0005750	cellular_component	mitochondrial respiratory chain complex III
J	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
J	0007584	biological_process	response to nutrient
J	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
J	0009055	molecular_function	electron transfer activity
J	0009410	biological_process	response to xenobiotic stimulus
J	0009636	biological_process	response to toxic substance
J	0009725	biological_process	response to hormone
J	0010243	biological_process	response to organonitrogen compound
J	0014070	biological_process	response to organic cyclic compound
J	0015990	biological_process	electron transport coupled proton transport
J	0016020	cellular_component	membrane
J	0016491	molecular_function	oxidoreductase activity
J	0022904	biological_process	respiratory electron transport chain
J	0031100	biological_process	animal organ regeneration
J	0032991	cellular_component	protein-containing complex
J	0033590	biological_process	response to cobalamin
J	0033762	biological_process	response to glucagon
J	0044877	molecular_function	protein-containing complex binding
J	0045275	cellular_component	respiratory chain complex III
J	0045333	biological_process	cellular respiration
J	0045471	biological_process	response to ethanol
J	0046686	biological_process	response to cadmium ion
J	0046688	biological_process	response to copper ion
J	0046689	biological_process	response to mercury ion
J	0046872	molecular_function	metal ion binding
J	0051592	biological_process	response to calcium ion
J	0055093	biological_process	response to hyperoxia
J	0070469	cellular_component	respirasome
K	0004222	molecular_function	metalloendopeptidase activity
K	0005515	molecular_function	protein binding
K	0005654	cellular_component	nucleoplasm
K	0005739	cellular_component	mitochondrion
K	0005743	cellular_component	mitochondrial inner membrane
K	0005750	cellular_component	mitochondrial respiratory chain complex III
K	0005751	cellular_component	mitochondrial respiratory chain complex IV
K	0006119	biological_process	oxidative phosphorylation
K	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
K	0006508	biological_process	proteolysis
K	0009060	biological_process	aerobic respiration
K	0016020	cellular_component	membrane
K	0045333	biological_process	cellular respiration
K	0046872	molecular_function	metal ion binding
K	0070469	cellular_component	respirasome
L	0005515	molecular_function	protein binding
L	0005739	cellular_component	mitochondrion
L	0005743	cellular_component	mitochondrial inner membrane
L	0005746	cellular_component	mitochondrial respirasome
L	0005750	cellular_component	mitochondrial respiratory chain complex III
L	0006119	biological_process	oxidative phosphorylation
L	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
L	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
L	0009060	biological_process	aerobic respiration
L	0014823	biological_process	response to activity
L	0016020	cellular_component	membrane
L	0031625	molecular_function	ubiquitin protein ligase binding
L	0043279	biological_process	response to alkaloid
L	0044877	molecular_function	protein-containing complex binding
L	0045333	biological_process	cellular respiration
L	0046872	molecular_function	metal ion binding
L	0070469	cellular_component	respirasome
L	1902600	biological_process	proton transmembrane transport
N	0005739	cellular_component	mitochondrion
N	0005743	cellular_component	mitochondrial inner membrane
N	0005750	cellular_component	mitochondrial respiratory chain complex III
N	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
N	0016020	cellular_component	membrane
N	0021539	biological_process	subthalamus development
N	0021548	biological_process	pons development
N	0021680	biological_process	cerebellar Purkinje cell layer development
N	0021766	biological_process	hippocampus development
N	0021794	biological_process	thalamus development
N	0021854	biological_process	hypothalamus development
N	0021860	biological_process	pyramidal neuron development
N	0030901	biological_process	midbrain development
N	0045333	biological_process	cellular respiration
N	0070469	cellular_component	respirasome
O	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
P	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
P	0016020	cellular_component	membrane
P	0051537	molecular_function	2 iron, 2 sulfur cluster binding
Q	0005515	molecular_function	protein binding
Q	0005739	cellular_component	mitochondrion
Q	0005743	cellular_component	mitochondrial inner membrane
Q	0005750	cellular_component	mitochondrial respiratory chain complex III
Q	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
Q	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
Q	0016020	cellular_component	membrane
Q	0045333	biological_process	cellular respiration
Q	0070469	cellular_component	respirasome
Q	1902600	biological_process	proton transmembrane transport
R	0005750	cellular_component	mitochondrial respiratory chain complex III
R	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
S	0005515	molecular_function	protein binding
S	0005739	cellular_component	mitochondrion
S	0005743	cellular_component	mitochondrial inner membrane
S	0005746	cellular_component	mitochondrial respirasome
S	0005750	cellular_component	mitochondrial respiratory chain complex III
S	0006119	biological_process	oxidative phosphorylation
S	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
S	0009060	biological_process	aerobic respiration
S	0016020	cellular_component	membrane
S	0045333	biological_process	cellular respiration
S	0070469	cellular_component	respirasome
T	0005739	cellular_component	mitochondrion
T	0005743	cellular_component	mitochondrial inner membrane
T	0005750	cellular_component	mitochondrial respiratory chain complex III
T	0006091	biological_process	generation of precursor metabolites and energy
T	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
T	0009055	molecular_function	electron transfer activity
T	0016020	cellular_component	membrane
T	0045333	biological_process	cellular respiration
T	0070469	cellular_component	respirasome
U	0009055	molecular_function	electron transfer activity
U	0020037	molecular_function	heme binding
V	0001666	biological_process	response to hypoxia
V	0005739	cellular_component	mitochondrion
V	0005743	cellular_component	mitochondrial inner membrane
V	0005750	cellular_component	mitochondrial respiratory chain complex III
V	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
V	0007584	biological_process	response to nutrient
V	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
V	0009055	molecular_function	electron transfer activity
V	0009410	biological_process	response to xenobiotic stimulus
V	0009636	biological_process	response to toxic substance
V	0009725	biological_process	response to hormone
V	0010243	biological_process	response to organonitrogen compound
V	0014070	biological_process	response to organic cyclic compound
V	0015990	biological_process	electron transport coupled proton transport
V	0016020	cellular_component	membrane
V	0016491	molecular_function	oxidoreductase activity
V	0022904	biological_process	respiratory electron transport chain
V	0031100	biological_process	animal organ regeneration
V	0032991	cellular_component	protein-containing complex
V	0033590	biological_process	response to cobalamin
V	0033762	biological_process	response to glucagon
V	0044877	molecular_function	protein-containing complex binding
V	0045275	cellular_component	respiratory chain complex III
V	0045333	biological_process	cellular respiration
V	0045471	biological_process	response to ethanol
V	0046686	biological_process	response to cadmium ion
V	0046688	biological_process	response to copper ion
V	0046689	biological_process	response to mercury ion
V	0046872	molecular_function	metal ion binding
V	0051592	biological_process	response to calcium ion
V	0055093	biological_process	response to hyperoxia
V	0070469	cellular_component	respirasome
W	0004222	molecular_function	metalloendopeptidase activity
W	0005515	molecular_function	protein binding
W	0005654	cellular_component	nucleoplasm
W	0005739	cellular_component	mitochondrion
W	0005743	cellular_component	mitochondrial inner membrane
W	0005750	cellular_component	mitochondrial respiratory chain complex III
W	0005751	cellular_component	mitochondrial respiratory chain complex IV
W	0006119	biological_process	oxidative phosphorylation
W	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
W	0006508	biological_process	proteolysis
W	0009060	biological_process	aerobic respiration
W	0016020	cellular_component	membrane
W	0045333	biological_process	cellular respiration
W	0046872	molecular_function	metal ion binding
W	0070469	cellular_component	respirasome
Y	0005515	molecular_function	protein binding
Y	0005739	cellular_component	mitochondrion
Y	0005743	cellular_component	mitochondrial inner membrane
Y	0005746	cellular_component	mitochondrial respirasome
Y	0005750	cellular_component	mitochondrial respiratory chain complex III
Y	0006119	biological_process	oxidative phosphorylation
Y	0006122	biological_process	mitochondrial electron transport, ubiquinol to cytochrome c
Y	0008121	molecular_function	ubiquinol-cytochrome-c reductase activity
Y	0009060	biological_process	aerobic respiration
Y	0014823	biological_process	response to activity
Y	0016020	cellular_component	membrane
Y	0031625	molecular_function	ubiquitin protein ligase binding
Y	0043279	biological_process	response to alkaloid
Y	0044877	molecular_function	protein-containing complex binding
Y	0045333	biological_process	cellular respiration
Y	0046872	molecular_function	metal ion binding
Y	0070469	cellular_component	respirasome
Y	1902600	biological_process	proton transmembrane transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	8
Details	binding site for residue CDL A 101

Chain	Residue
A	ARG41
A	ILE42
A	SER45
F	HIS73
H	CDL403
J	ALA29
J	PHE33
J	PEE403

---

site_id	AC2
Number of Residues	6
Details	binding site for residue FES C 301

Chain	Residue
C	HIS219
C	LEU220
C	CYS236
C	HIS239
C	SER241
C	CYS217

---

site_id	AC3
Number of Residues	8
Details	binding site for residue CDL G 101

Chain	Residue
C	VAL137
C	SER141
G	ASP37
G	TRP38
G	ARG39
V	TYR155
V	ILE156
V	TRP163

---

site_id	AC4
Number of Residues	10
Details	binding site for residue PEE H 401

Chain	Residue
C	ASN131
C	ALA132
C	PHE136
D	ASP37
H	HIS284
H	MET288
H	LYS291
J	LEU236
J	MET240
L	PLX501

---

site_id	AC5
Number of Residues	17
Details	binding site for residue HEC H 402

Chain	Residue
H	VAL120
H	CYS121
H	CYS124
H	HIS125
H	ASN189
H	LEU197
H	ILE200
H	ARG204
H	TYR210
H	LEU214
H	PHE237
H	ILE242
H	ALA243
H	MET244
H	PRO247
H	VAL270
P	HIS239

---

site_id	AC6
Number of Residues	14
Details	binding site for residue CDL H 403

Chain	Residue
A	ASN37
A	ARG41
A	CDL101
F	LYS72
F	HIS73
H	TYR304
H	LYS307
H	ARG308
H	LYS315
J	ASN32
J	LYS227
J	LEU230
J	LEU234
J	SER238

---

site_id	AC7
Number of Residues	15
Details	binding site for residue HEM J 401

Chain	Residue
J	LEU41
J	GLN44
J	ILE45
J	GLY48
J	LEU51
J	ALA52
J	ARG80
J	HIS83
J	GLY130
J	LEU133
J	PRO134
J	HIS182
J	PHE183
J	PRO186
J	TYR273

---

site_id	AC8
Number of Residues	16
Details	binding site for residue HEM J 402

Chain	Residue
J	TRP31
J	GLY34
J	SER35
J	LEU37
J	HIS97
J	ILE98
J	ARG100
J	SER106
J	TYR109
J	TRP113
J	GLY116
J	LEU119
J	HIS196
J	LEU200
J	SER205
J	ASN206

---

site_id	AC9
Number of Residues	15
Details	binding site for residue PEE J 403

Chain	Residue
J	PHE95
J	LEU96
J	GLY99
J	ARG100
J	TYR103
J	TYR104
J	MET316
J	TYR325
J	TRP326
J	LEU332
A	GLU44
A	VAL49
A	CDL101
J	TRP30
J	ILE92

---

site_id	AD1
Number of Residues	10
Details	binding site for residue CDL J 404

Chain	Residue
J	THR194
J	LEU195
J	LEU197
J	LEU198
J	HIS201
V	LEU13
V	SER17
V	THR194
V	LEU198
V	HIS201

---

site_id	AD2
Number of Residues	8
Details	binding site for residue CDL J 405

Chain	Residue
J	ASP159
J	LEU160
J	TRP163
P	THR134
P	VAL137
P	SER138
T	THR36
Y	PEE502

---

site_id	AD3
Number of Residues	10
Details	binding site for residue PLX L 501

Chain	Residue
C	TYR115
C	THR118
D	PHE15
D	THR18
D	PHE21
D	ALA22
H	PEE401
J	ALA229
L	PHE476
L	PEE503

---

site_id	AD4
Number of Residues	10
Details	binding site for residue CDL L 502

Chain	Residue
J	ARG5
J	PHE18
J	ILE19
J	HIS221
L	PHE370
L	ARG470
L	TRP477
L	LEU478
L	ARG479
L	PEE503

---

site_id	AD5
Number of Residues	5
Details	binding site for residue PEE L 503

Chain	Residue
J	HIS221
L	SER473
L	PHE476
L	PLX501
L	CDL502

---

site_id	AD6
Number of Residues	7
Details	binding site for residue CDL N 101

Chain	Residue
N	ARG41
N	SER45
S	HIS73
U	CDL403
V	ALA29
V	PHE33
V	PEE403

---

site_id	AD7
Number of Residues	8
Details	binding site for residue FES P 301

Chain	Residue
P	CYS217
P	HIS219
P	LEU220
P	CYS222
P	CYS236
P	CYS238
P	HIS239
P	SER241

---

site_id	AD8
Number of Residues	12
Details	binding site for residue PLX Q 101

Chain	Residue
P	TYR115
P	THR118
P	THR122
P	VAL125
Q	PHE15
Q	ARG16
Q	THR18
Q	PHE21
Q	ALA22
Y	PHE476
Y	CDL501
Y	PEE502

---

site_id	AD9
Number of Residues	3
Details	binding site for residue PLX T 101

Chain	Residue
T	ARG9
T	LEU13
Y	TRP477

---

site_id	AE1
Number of Residues	9
Details	binding site for residue PEE U 401

Chain	Residue
P	TYR127
P	ALA128
P	ASN131
U	MET288
U	LYS291
U	MET295
U	MET296
V	LEU236
V	MET240

---

site_id	AE2
Number of Residues	15
Details	binding site for residue HEC U 402

Chain	Residue
C	HIS239
U	VAL120
U	CYS121
U	CYS124
U	HIS125
U	ILE200
U	ARG204
U	TYR210
U	LEU214
U	LEU215
U	PHE237
U	ILE242
U	MET244
U	PRO247
U	VAL270

---

site_id	AE3
Number of Residues	11
Details	binding site for residue CDL U 403

Chain	Residue
N	VAL30
N	CDL101
U	PRO301
U	TYR304
U	LYS307
U	ARG308
U	LYS315
V	LEU36
V	LYS227
V	LEU230
V	LEU234

---

site_id	AE4
Number of Residues	14
Details	binding site for residue HEM V 401

Chain	Residue
V	LEU41
V	GLN44
V	ILE45
V	GLY48
V	LEU49
V	ARG80
V	HIS83
V	ALA87
V	PHE90
V	GLY130
V	HIS182
V	PHE183
V	PRO186
V	TYR273

---

site_id	AE5
Number of Residues	17
Details	binding site for residue HEM V 402

Chain	Residue
V	TRP31
V	GLY34
V	SER35
V	LEU37
V	GLY38
V	LEU94
V	HIS97
V	ILE98
V	SER106
V	TYR109
V	GLY116
V	LEU119
V	HIS196
V	LEU197
V	LEU200
V	SER205
V	ASN206

---

site_id	AE6
Number of Residues	13
Details	binding site for residue PEE V 403

Chain	Residue
N	VAL49
N	PHE53
N	CDL101
V	TRP30
V	PHE95
V	LEU96
V	TYR103
V	TYR104
V	MET316
V	TYR325
V	TRP326
V	LEU332
V	LEU333

---

site_id	AE7
Number of Residues	9
Details	binding site for residue CDL Y 501

Chain	Residue
Q	PLX101
T	TRP24
V	ARG5
V	HIS221
Y	PHE370
Y	TRP477
Y	LEU478
Y	ARG479
Y	PEE502

---

site_id	AE8
Number of Residues	10
Details	binding site for residue PEE Y 502

Chain	Residue
J	CDL405
P	ALA126
Q	PLX101
T	ALA35
V	LEU10
V	ILE14
V	HIS221
Y	SER473
Y	PHE476
Y	CDL501

---

Functional Information from PROSITE/UniProt

site_id	PS00143
Number of Residues	24
Details	INSULINASE Insulinase family, zinc-binding region signature. GsryedfsnlGtTHLLRLTsSlTT

Chain	Residue	Details
K	GLY68-THR91

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861

Chain	Residue	Details
L	LYS111
V	TRP77-ILE98
V	TRP113-LEU133
V	PHE178-LEU198
V	ILE226-SER246
V	LEU288-HIS308
V	LEU320-GLY340
V	PHE347-PRO367
Y	LYS111
K	LYS250
W	LYS66
W	LYS199
W	LYS250
J	LEU320-GLY340
J	PHE347-PRO367
V	PHE33-MET53

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q9CZ13

Chain	Residue	Details
L	LYS138
L	LYS248
Y	LYS138
Y	LYS248
V	HIS83
V	HIS97
V	HIS182
V	HIS196

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N6-succinyllysine; alternate => ECO:0000250|UniProtKB:Q9CZ13

Chain	Residue	Details
L	LYS163
Y	LYS163

---

site_id	SWS_FT_FI4
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:Q68FY0

Chain	Residue	Details
L	SER212
P	SER241
Y	SER212
U	CYS121
U	CYS124
C	SER241
P	CYS217
P	HIS219
P	CYS236
P	HIS239

---

site_id	SWS_FT_FI5
Number of Residues	2
Details	BINDING: axial binding residue => ECO:0000269|PubMed:28844695, ECO:0007744|PDB:5XTE, ECO:0007744|PDB:5XTH

Chain	Residue	Details
H	HIS125
U	HIS125

---

site_id	SWS_FT_FI6
Number of Residues	2
Details	BINDING: axial binding residue => ECO:0000269|PubMed:28844695, ECO:0007744|PDB:5XTH

Chain	Residue	Details
H	MET244
U	MET244

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163

Chain	Residue	Details
H	SER182
U	SER182

---